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The map label for this gene is leuE [H]

Identifier: 99082410

GI number: 99082410

Start: 2712248

End: 2712865

Strand: Direct

Name: leuE [H]

Synonym: TM1040_2570

Alternate gene names: 99082410

Gene position: 2712248-2712865 (Clockwise)

Preceding gene: 99082409

Following gene: 99082411

Centisome position: 84.73

GC content: 60.52

Gene sequence:

>618_bases
TTGATTTCCCTGCAATTTCTCCTGACCGCATTTGTCGTGGTCCTCGCGCCGGGTACCGGTGTGATCTACACTCTGGCTTT
GGGTCTGGGGCGCGGACGCCGGGCCGCCCTCTGGGCCGCAGTAGGCTGTACTTTCGGTATCCTGCCGCATCTGATGGCCG
CCACACTGGGGCTTGCGGCGGTGCTGCATTCCTCGGCCGTGCTGTTCCAAGGCATCAAGATCGCAGGTGTTCTCTACTTG
CTCTACCTCGCTTGGCAGATGCTGAAATCCGATGGCGCGCTCTCGGTCTCTGCGGCACAGCCTGCCTCACAGCCGGGGAT
TACGATCGCCAAACGTGGCGCCCTGATCAACATTCTGAATCCCAAGCTCTCGATCTTCTTTCTGGCACTGCTGCCGCCGT
TCCTCTCCGGCAATACAGCCACCGCCACGCAGGAAATGGTCGCACTTGGTGGGATCTTCATGGCGATGACCTTTGCCGTC
TTTGCGCTCTACGGTCTTTTTGCCGCGCAGGCCCGCAGCCTCATTCTTGGCTCCGAGCGCGTAGTGACATGGCTCAATCG
CAGCTTTGCCGCCATCTTTGCAGCTCTCGCGCTGCGCTTGGCAATGGAGCGCAGCTGA

Upstream 100 bases:

>100_bases
CTCTCGGGGCGCGAAGGGTGCTTGCAACTTCACCACGCGTGGCTACCTTGAAAGACACGCACCTAGGGCGTCACACGTCG
TTTTCAGAGGAACAGCTGCT

Downstream 100 bases:

>100_bases
TGTTTCGCGGCATTCCCGCAGCGCCGCTCGCGCTTGGCCTCGCTGGCCTCATTCCTTTTGTGTGGGGCGCCCTGACGCTG
CTGGTGCCTGCATTGCAGTC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MISLQFLLTAFVVVLAPGTGVIYTLALGLGRGRRAALWAAVGCTFGILPHLMAATLGLAAVLHSSAVLFQGIKIAGVLYL
LYLAWQMLKSDGALSVSAAQPASQPGITIAKRGALINILNPKLSIFFLALLPPFLSGNTATATQEMVALGGIFMAMTFAV
FALYGLFAAQARSLILGSERVVTWLNRSFAAIFAALALRLAMERS

Sequences:

>Translated_205_residues
MISLQFLLTAFVVVLAPGTGVIYTLALGLGRGRRAALWAAVGCTFGILPHLMAATLGLAAVLHSSAVLFQGIKIAGVLYL
LYLAWQMLKSDGALSVSAAQPASQPGITIAKRGALINILNPKLSIFFLALLPPFLSGNTATATQEMVALGGIFMAMTFAV
FALYGLFAAQARSLILGSERVVTWLNRSFAAIFAALALRLAMERS
>Mature_205_residues
MISLQFLLTAFVVVLAPGTGVIYTLALGLGRGRRAALWAAVGCTFGILPHLMAATLGLAAVLHSSAVLFQGIKIAGVLYL
LYLAWQMLKSDGALSVSAAQPASQPGITIAKRGALINILNPKLSIFFLALLPPFLSGNTATATQEMVALGGIFMAMTFAV
FALYGLFAAQARSLILGSERVVTWLNRSFAAIFAALALRLAMERS

Specific function: Exporter of leucine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=199, Percent_Identity=28.1407035175879, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994978, Length=215, Percent_Identity=27.4418604651163, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1786522, Length=193, Percent_Identity=25.9067357512953, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21556; Mature: 21556

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLQFLLTAFVVVLAPGTGVIYTLALGLGRGRRAALWAAVGCTFGILPHLMAATLGLAA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLHSSAVLFQGIKIAGVLYLLYLAWQMLKSDGALSVSAAQPASQPGITIAKRGALINILN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCEEEECC
PKLSIFFLALLPPFLSGNTATATQEMVALGGIFMAMTFAVFALYGLFAAQARSLILGSER
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VVTWLNRSFAAIFAALALRLAMERS
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISLQFLLTAFVVVLAPGTGVIYTLALGLGRGRRAALWAAVGCTFGILPHLMAATLGLAA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLHSSAVLFQGIKIAGVLYLLYLAWQMLKSDGALSVSAAQPASQPGITIAKRGALINILN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCEEEECC
PKLSIFFLALLPPFLSGNTATATQEMVALGGIFMAMTFAVFALYGLFAAQARSLILGSER
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VVTWLNRSFAAIFAALALRLAMERS
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA