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The map label for this gene is ispH

Identifier: 99082409

GI number: 99082409

Start: 2711132

End: 2712082

Strand: Direct

Name: ispH

Synonym: TM1040_2569

Alternate gene names: 99082409

Gene position: 2711132-2712082 (Clockwise)

Preceding gene: 99082408

Following gene: 99082410

Centisome position: 84.7

GC content: 63.3

Gene sequence:

>951_bases
ATGGCCGCCAAACCACCCCTCACCCTCTATCTTGCAGCTCCGCGTGGCTTTTGCGCAGGTGTCGACCGGGCCATCAAGAT
CGTGGAGATGGCGCTCGAGAAATGGGGCGCTCCGGTCTATGTGCGCCATGAGATCGTTCACAACAAGTTTGTGGTCGACG
GGCTGCGGGACAAGGGCGCGGTCTTTGTCGAGGAACTGGAGGAATGCCCGGACGACCGGCCGGTGATTTTCTCTGCCCAT
GGCGTGCCGAAATCCGTGCCGGCCGCCGCCGAGGCGCGCCAGATGGTCTATGTCGACGCCACCTGCCCGCTTGTCTCAAA
GGTTCATATCGAGGCCCAGCGCCACGCCGAAGCCGGGCTTCAAATGATCATGATCGGTCACAAAGGGCACCCGGAGACGG
TTGGCACCATGGGTCAACTGCCCGAAGGAGAGGTCTTGCTGGTCGAAACCGTCGAGGATGTGGCCCAGGTGGAGGTCCGC
GACCCGGAGCGGCTCGCCTTTGTCACTCAGACCACCCTCTCGGTGGATGACACCAAAGACATCGTGGCGGCGCTGCAAGC
GCGTTTCCCGGCCATCGTCGGCCCCCACAAGGAAGACATCTGCTACGCCACCACCAATCGGCAAGAAGCCGTGAAAGAAG
TGGCCCCCAAAGCAGACGCACTCTTGGTGGTCGGTGCGCCGAACTCGTCGAACTCGCGCCGACTGGTCGAGGTCGGGGCC
AAGAATGGCTGCAGCTATGCGCAGCTGGTACAGCGGGCCGAAAACATCGACTGGCGCGCGCTGGAGGGCATCCAGAGCAT
CGCCATCACTGCGGGCGCCTCTGCGCCCGAGCTATTGGTGAACGAGGTGATCGACGCCTTTCGCGACCGCTATGAGGTGA
CGGTGGAGCTGGTTGAGACCGCCGTGGAGCGGGTGGAGTTCAAAGTGCCGCGTGTCCTGCGCACGGCCTGA

Upstream 100 bases:

>100_bases
TTTCTTTTGCAGCCCTGCCCGACCGGCACTGGACCTTGAACGGTGCAAGGCTTACCTCTGATCCAAGACGCTCACATCTG
ATTTGCGGAGACCCGCGCCC

Downstream 100 bases:

>100_bases
GGCCACCTCAAGCTCCAAGCAAGACCTACCTCAGACCCCCATGAGACCAGACTGGCAATGCGCCACTCTCGGGGCGCGAA
GGGTGCTTGCAACTTCACCA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAAKPPLTLYLAAPRGFCAGVDRAIKIVEMALEKWGAPVYVRHEIVHNKFVVDGLRDKGAVFVEELEECPDDRPVIFSAH
GVPKSVPAAAEARQMVYVDATCPLVSKVHIEAQRHAEAGLQMIMIGHKGHPETVGTMGQLPEGEVLLVETVEDVAQVEVR
DPERLAFVTQTTLSVDDTKDIVAALQARFPAIVGPHKEDICYATTNRQEAVKEVAPKADALLVVGAPNSSNSRRLVEVGA
KNGCSYAQLVQRAENIDWRALEGIQSIAITAGASAPELLVNEVIDAFRDRYEVTVELVETAVERVEFKVPRVLRTA

Sequences:

>Translated_316_residues
MAAKPPLTLYLAAPRGFCAGVDRAIKIVEMALEKWGAPVYVRHEIVHNKFVVDGLRDKGAVFVEELEECPDDRPVIFSAH
GVPKSVPAAAEARQMVYVDATCPLVSKVHIEAQRHAEAGLQMIMIGHKGHPETVGTMGQLPEGEVLLVETVEDVAQVEVR
DPERLAFVTQTTLSVDDTKDIVAALQARFPAIVGPHKEDICYATTNRQEAVKEVAPKADALLVVGAPNSSNSRRLVEVGA
KNGCSYAQLVQRAENIDWRALEGIQSIAITAGASAPELLVNEVIDAFRDRYEVTVELVETAVERVEFKVPRVLRTA
>Mature_315_residues
AAKPPLTLYLAAPRGFCAGVDRAIKIVEMALEKWGAPVYVRHEIVHNKFVVDGLRDKGAVFVEELEECPDDRPVIFSAHG
VPKSVPAAAEARQMVYVDATCPLVSKVHIEAQRHAEAGLQMIMIGHKGHPETVGTMGQLPEGEVLLVETVEDVAQVEVRD
PERLAFVTQTTLSVDDTKDIVAALQARFPAIVGPHKEDICYATTNRQEAVKEVAPKADALLVVGAPNSSNSRRLVEVGAK
NGCSYAQLVQRAENIDWRALEGIQSIAITAGASAPELLVNEVIDAFRDRYEVTVELVETAVERVEFKVPRVLRTA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=51.6129032258064, Blast_Score=320, Evalue=8e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_SILST (Q1GDG5)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614563.1
- STRING:   Q1GDG5
- GeneID:   4076700
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2569
- NMPDR:   fig|292414.1.peg.715
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   WGAPVYV
- PhylomeDB:   Q1GDG5
- ProtClustDB:   PRK01045
- BioCyc:   SSP292414:TM1040_2569-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34428; Mature: 34297

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKPPLTLYLAAPRGFCAGVDRAIKIVEMALEKWGAPVYVRHEIVHNKFVVDGLRDKGA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHEEECCCCCCH
VFVEELEECPDDRPVIFSAHGVPKSVPAAAEARQMVYVDATCPLVSKVHIEAQRHAEAGL
HHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCC
QMIMIGHKGHPETVGTMGQLPEGEVLLVETVEDVAQVEVRDPERLAFVTQTTLSVDDTKD
EEEEEECCCCCCHHCCCCCCCCCCEEEEEEHHHHHHEECCCCHHEEEEEEEEECCCCHHH
IVAALQARFPAIVGPHKEDICYATTNRQEAVKEVAPKADALLVVGAPNSSNSRRLVEVGA
HHHHHHHHCCHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECC
KNGCSYAQLVQRAENIDWRALEGIQSIAITAGASAPELLVNEVIDAFRDRYEVTVELVET
CCCCCHHHHHHHHHCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AVERVEFKVPRVLRTA
HHHHHHHHCCHHHHCC
>Mature Secondary Structure 
AAKPPLTLYLAAPRGFCAGVDRAIKIVEMALEKWGAPVYVRHEIVHNKFVVDGLRDKGA
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHEEECCCCCCH
VFVEELEECPDDRPVIFSAHGVPKSVPAAAEARQMVYVDATCPLVSKVHIEAQRHAEAGL
HHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCC
QMIMIGHKGHPETVGTMGQLPEGEVLLVETVEDVAQVEVRDPERLAFVTQTTLSVDDTKD
EEEEEECCCCCCHHCCCCCCCCCCEEEEEEHHHHHHEECCCCHHEEEEEEEEECCCCHHH
IVAALQARFPAIVGPHKEDICYATTNRQEAVKEVAPKADALLVVGAPNSSNSRRLVEVGA
HHHHHHHHCCHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECC
KNGCSYAQLVQRAENIDWRALEGIQSIAITAGASAPELLVNEVIDAFRDRYEVTVELVET
CCCCCHHHHHHHHHCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AVERVEFKVPRVLRTA
HHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA