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The map label for this gene is rsh [H]

Identifier: 99082405

GI number: 99082405

Start: 2707049

End: 2709181

Strand: Reverse

Name: rsh [H]

Synonym: TM1040_2565

Alternate gene names: 99082405

Gene position: 2709181-2707049 (Counterclockwise)

Preceding gene: 99082406

Following gene: 99082404

Centisome position: 84.64

GC content: 60.38

Gene sequence:

>2133_bases
ATGACATCTGCTGATGACCTGATTTCCCTGGTCCGCGACTACAACCCCAAAACGAATGAGCAGCGCCTGCGCGACGCCTA
CGCCTTTGGCGAGGAAATGCATGACGGGCAGTTTCGCCATTCCGGGGAACCCTATTTCACACACCCTGTCGCAGTCGCCG
CAATCCTGACCGAACAGCGGCTCGATGATGCAACCATCATCACGGCCCTGCTGCACGACACGATCGAGGACACCAAGGCC
TCTTACGAGGAAGTGTCGTTGCGTTTTGGCGAAGAAGTGGCGATGCTGGTCGATGGTGTCACGAAGCTGACGAACCTGCA
GCTCTCGTCGCGCGAAACCAAACAGGCGGAGAACTTTCGCAAGCTGTTTATGGCGATGTCGAAGGATCTTCGGGTCATTC
TCGTCAAGCTGGCCGACCGACTGCACAACATGCGCACCATTCGTGCCATGCGCCCGGAAAAACAGGCGCAAAAAGCCCGC
GAAACCATGGACATCTACGCCCCCCTCGCGGGGCGGATGGGGATGCAGTGGATGCGCGAGGAACTGGAAGACCTGGCCTT
CAAGGTGCTGAACCCCGAAGGTCGCCAGTCGATTATCCGCCGCTTTGTGACCCTGCAGCGCGAGACCGGGGACGTGATCC
ATCGGATTACCGGCGACATGAGGCTGGAGTTGGAGAAGGCGGGCGTTGAGGCCGAAGTCTTTGGCCGTGCAAAGAAACCC
TATTCGATCTGGCGCAAGATGCAGGAAAAGGATCAGGGGTTCTCGCGTCTCTCGGACATTTACGGCTTTCGCGTGATCAC
GCGCTCCGAAGAGGACTGCTATCGCACGCTTGGCACGATCCACCAGCGCTGGCGGGCGGTGCCGGGGCGGTTCAAGGATT
ATATCAGCCAGCCCAAATCCAACGGCTATCGCTCGATCCACACCACGGTGTCGGGACGCGACGGCAAGCGGGTCGAGGTG
CAGATCCGCACCCGGCAGATGCATGATGTTGCGGAATCGGGTGTGGCGGCGCATTGGTCCTATCGCGATGGTGTGCGCAC
CGAGAACCCCTTTGCCGTTGATCCCGCCAAGTGGATCGCCTCGCTCACTGAACAGTTTGATGGTGAAGACGATCACGACG
AATTCCTCGAAGCGGTGAAGCTCGAGATGTATTCGGATCAGGTGTTCTGCTTCACGCCCAAGGGCGACGTGATCAAGCTG
CCCAAGGGTGCCACACCGATTGATTTTGCCTATGCGATCCACACGCGTATCGGCCACGCCTGTGTCGGCGCCAAGGTAGA
CGGCATCCGGGTGCCGCTCTGGACCCGGCTCAGAAATGGTCAGTCGGTGGAAGTGATCACCGCCGAAGGCCAGACTCCGC
AGGTCAGCTGGCTGGAAATTGCCACCACTGGCAAGGCGCGCACGGCTATTCGCCGGGCCCTACGGGAGGCGGACCGGGAA
CGGTTTGTAAAACTCGGCCACGAGCTGGCGCGTTCCGCGTTTGAGCACATGAACAAGAAGGCCACCGACAAGGCGCTGGA
AACTGCGGCCCGCGCGTTGCGGGTCAGTGATGTGACCGAATTGCTGGCGCGCCTCGGGGCTGCGGTGATCACCGCGCATG
ACGTGGTGCAGGCCGTCTACCCCGAGCTTACCGATAAGGACGGCGAGGAAGTCTCGCCCCGGCGCGCCGTGATCGGCCTG
GAACCGGGCCAGAAGTTCGAACGCGCTTCCTGCTGCGAGCCGCTGCCGGGAGAGCGCATCATCGGGATCACTTCGCGTGG
GCAGGGTGTCGTGGTACACTCGGCGGACTGTGACCGGCTCGGAGAGTTCGAGGACCAGATGGACCGGTGGGTCGATGTGC
ATTGGCACTCGGGCACCCATCCCGCTGTCTATGGCGTCACACTTGAATTGACGATCGGGAACGACGCAGGCGTTCTGGGG
CGCATCTGTACGTTGATCGGCGAAAAGAAAGCCAACATTTCGAACCTTGAATTCAAGGATCGGAAACCTGACTTTTACCG
CTTGATGATCAATGTGGAGCTGCGCGATGTGGAACAGCTGCATTCGCTGATACTGATGTTGGAAGCTGAAAGCGACGTCG
CCGCGGTCGCGCGCTACCGGGAAACGCCTGGAACGCAAGCCCGCACGATCTAG

Upstream 100 bases:

>100_bases
AAGACGACATGAGCGAAGAAAAGCTCCTGCGCGCCTTGATGGAGGCCCAGGGTCAGGGCTGATCCGGGCTGCTTGAAACA
AGAGAGGTTGCGGACCGGCA

Downstream 100 bases:

>100_bases
CCTCGTTAGACGAAGAATAGGGACGATTCTTTGGTATTCAGGCGCCGAGACAGACGCCCGCCGCTACGGGCGGCTGCGGA
GTTTCTCTGGCCTCGCGGGG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MTSADDLISLVRDYNPKTNEQRLRDAYAFGEEMHDGQFRHSGEPYFTHPVAVAAILTEQRLDDATIITALLHDTIEDTKA
SYEEVSLRFGEEVAMLVDGVTKLTNLQLSSRETKQAENFRKLFMAMSKDLRVILVKLADRLHNMRTIRAMRPEKQAQKAR
ETMDIYAPLAGRMGMQWMREELEDLAFKVLNPEGRQSIIRRFVTLQRETGDVIHRITGDMRLELEKAGVEAEVFGRAKKP
YSIWRKMQEKDQGFSRLSDIYGFRVITRSEEDCYRTLGTIHQRWRAVPGRFKDYISQPKSNGYRSIHTTVSGRDGKRVEV
QIRTRQMHDVAESGVAAHWSYRDGVRTENPFAVDPAKWIASLTEQFDGEDDHDEFLEAVKLEMYSDQVFCFTPKGDVIKL
PKGATPIDFAYAIHTRIGHACVGAKVDGIRVPLWTRLRNGQSVEVITAEGQTPQVSWLEIATTGKARTAIRRALREADRE
RFVKLGHELARSAFEHMNKKATDKALETAARALRVSDVTELLARLGAAVITAHDVVQAVYPELTDKDGEEVSPRRAVIGL
EPGQKFERASCCEPLPGERIIGITSRGQGVVVHSADCDRLGEFEDQMDRWVDVHWHSGTHPAVYGVTLELTIGNDAGVLG
RICTLIGEKKANISNLEFKDRKPDFYRLMINVELRDVEQLHSLILMLEAESDVAAVARYRETPGTQARTI

Sequences:

>Translated_710_residues
MTSADDLISLVRDYNPKTNEQRLRDAYAFGEEMHDGQFRHSGEPYFTHPVAVAAILTEQRLDDATIITALLHDTIEDTKA
SYEEVSLRFGEEVAMLVDGVTKLTNLQLSSRETKQAENFRKLFMAMSKDLRVILVKLADRLHNMRTIRAMRPEKQAQKAR
ETMDIYAPLAGRMGMQWMREELEDLAFKVLNPEGRQSIIRRFVTLQRETGDVIHRITGDMRLELEKAGVEAEVFGRAKKP
YSIWRKMQEKDQGFSRLSDIYGFRVITRSEEDCYRTLGTIHQRWRAVPGRFKDYISQPKSNGYRSIHTTVSGRDGKRVEV
QIRTRQMHDVAESGVAAHWSYRDGVRTENPFAVDPAKWIASLTEQFDGEDDHDEFLEAVKLEMYSDQVFCFTPKGDVIKL
PKGATPIDFAYAIHTRIGHACVGAKVDGIRVPLWTRLRNGQSVEVITAEGQTPQVSWLEIATTGKARTAIRRALREADRE
RFVKLGHELARSAFEHMNKKATDKALETAARALRVSDVTELLARLGAAVITAHDVVQAVYPELTDKDGEEVSPRRAVIGL
EPGQKFERASCCEPLPGERIIGITSRGQGVVVHSADCDRLGEFEDQMDRWVDVHWHSGTHPAVYGVTLELTIGNDAGVLG
RICTLIGEKKANISNLEFKDRKPDFYRLMINVELRDVEQLHSLILMLEAESDVAAVARYRETPGTQARTI
>Mature_709_residues
TSADDLISLVRDYNPKTNEQRLRDAYAFGEEMHDGQFRHSGEPYFTHPVAVAAILTEQRLDDATIITALLHDTIEDTKAS
YEEVSLRFGEEVAMLVDGVTKLTNLQLSSRETKQAENFRKLFMAMSKDLRVILVKLADRLHNMRTIRAMRPEKQAQKARE
TMDIYAPLAGRMGMQWMREELEDLAFKVLNPEGRQSIIRRFVTLQRETGDVIHRITGDMRLELEKAGVEAEVFGRAKKPY
SIWRKMQEKDQGFSRLSDIYGFRVITRSEEDCYRTLGTIHQRWRAVPGRFKDYISQPKSNGYRSIHTTVSGRDGKRVEVQ
IRTRQMHDVAESGVAAHWSYRDGVRTENPFAVDPAKWIASLTEQFDGEDDHDEFLEAVKLEMYSDQVFCFTPKGDVIKLP
KGATPIDFAYAIHTRIGHACVGAKVDGIRVPLWTRLRNGQSVEVITAEGQTPQVSWLEIATTGKARTAIRRALREADRER
FVKLGHELARSAFEHMNKKATDKALETAARALRVSDVTELLARLGAAVITAHDVVQAVYPELTDKDGEEVSPRRAVIGLE
PGQKFERASCCEPLPGERIIGITSRGQGVVVHSADCDRLGEFEDQMDRWVDVHWHSGTHPAVYGVTLELTIGNDAGVLGR
ICTLIGEKKANISNLEFKDRKPDFYRLMINVELRDVEQLHSLILMLEAESDVAAVARYRETPGTQARTI

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=140, Percent_Identity=38.5714285714286, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790082, Length=706, Percent_Identity=39.8016997167139, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1789147, Length=678, Percent_Identity=32.4483775811209, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25143118, Length=160, Percent_Identity=35, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24650996, Length=149, Percent_Identity=32.8859060402685, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 80421; Mature: 80290

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSADDLISLVRDYNPKTNEQRLRDAYAFGEEMHDGQFRHSGEPYFTHPVAVAAILTEQR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHC
LDDATIITALLHDTIEDTKASYEEVSLRFGEEVAMLVDGVTKLTNLQLSSRETKQAENFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KLFMAMSKDLRVILVKLADRLHNMRTIRAMRPEKQAQKARETMDIYAPLAGRMGMQWMRE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ELEDLAFKVLNPEGRQSIIRRFVTLQRETGDVIHRITGDMRLELEKAGVEAEVFGRAKKP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCHHHHCCCCCC
YSIWRKMQEKDQGFSRLSDIYGFRVITRSEEDCYRTLGTIHQRWRAVPGRFKDYISQPKS
HHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
NGYRSIHTTVSGRDGKRVEVQIRTRQMHDVAESGVAAHWSYRDGVRTENPFAVDPAKWIA
CCCEEEEEEECCCCCCEEEEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHH
SLTEQFDGEDDHDEFLEAVKLEMYSDQVFCFTPKGDVIKLPKGATPIDFAYAIHTRIGHA
HHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHH
CVGAKVDGIRVPLWTRLRNGQSVEVITAEGQTPQVSWLEIATTGKARTAIRRALREADRE
HHCCCCCCEECCHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
RFVKLGHELARSAFEHMNKKATDKALETAARALRVSDVTELLARLGAAVITAHDVVQAVY
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PELTDKDGEEVSPRRAVIGLEPGQKFERASCCEPLPGERIIGITSRGQGVVVHSADCDRL
HHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCHHH
GEFEDQMDRWVDVHWHSGTHPAVYGVTLELTIGNDAGVLGRICTLIGEKKANISNLEFKD
CHHHHHHHHEEEEEECCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCC
RKPDFYRLMINVELRDVEQLHSLILMLEAESDVAAVARYRETPGTQARTI
CCCCEEEEEEEEEHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TSADDLISLVRDYNPKTNEQRLRDAYAFGEEMHDGQFRHSGEPYFTHPVAVAAILTEQR
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHC
LDDATIITALLHDTIEDTKASYEEVSLRFGEEVAMLVDGVTKLTNLQLSSRETKQAENFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KLFMAMSKDLRVILVKLADRLHNMRTIRAMRPEKQAQKARETMDIYAPLAGRMGMQWMRE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ELEDLAFKVLNPEGRQSIIRRFVTLQRETGDVIHRITGDMRLELEKAGVEAEVFGRAKKP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCHHHHCCCCCC
YSIWRKMQEKDQGFSRLSDIYGFRVITRSEEDCYRTLGTIHQRWRAVPGRFKDYISQPKS
HHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
NGYRSIHTTVSGRDGKRVEVQIRTRQMHDVAESGVAAHWSYRDGVRTENPFAVDPAKWIA
CCCEEEEEEECCCCCCEEEEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHH
SLTEQFDGEDDHDEFLEAVKLEMYSDQVFCFTPKGDVIKLPKGATPIDFAYAIHTRIGHA
HHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHH
CVGAKVDGIRVPLWTRLRNGQSVEVITAEGQTPQVSWLEIATTGKARTAIRRALREADRE
HHCCCCCCEECCHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
RFVKLGHELARSAFEHMNKKATDKALETAARALRVSDVTELLARLGAAVITAHDVVQAVY
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PELTDKDGEEVSPRRAVIGLEPGQKFERASCCEPLPGERIIGITSRGQGVVVHSADCDRL
HHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCHHH
GEFEDQMDRWVDVHWHSGTHPAVYGVTLELTIGNDAGVLGRICTLIGEKKANISNLEFKD
CHHHHHHHHEEEEEECCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCC
RKPDFYRLMINVELRDVEQLHSLILMLEAESDVAAVARYRETPGTQARTI
CCCCEEEEEEEEEHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA