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The map label for this gene is dacF [H]

Identifier: 99082376

GI number: 99082376

Start: 2676295

End: 2677785

Strand: Direct

Name: dacF [H]

Synonym: TM1040_2536

Alternate gene names: 99082376

Gene position: 2676295-2677785 (Clockwise)

Preceding gene: 99082375

Following gene: 99082386

Centisome position: 83.61

GC content: 61.17

Gene sequence:

>1491_bases
ATGGTCATGGATGCGCGTACCGGCGAAGTGCTGCATTCGCGCAATGCGGATACTCGGCTGCACCCCGCCTCGCTGACCAA
GATGATGACCCTATACATCGCCTTTGAGGCGGTGCGGAACGGGGAAATCACCCTCGACACCCAGATCACGGTTTCGCGCA
ATGCCGAGGCAGAACCTCCTTCTGAGTTGGGCCTGCGCCGCGGGCAAAAAATCGCACTACGGTATCTGATCCGGGCCGCA
GCGGTAAAATCCGCCAATGATGCGGCCACGGCCATTGGTGAGGCGATCTCCGGGTCTGAGGCGGCCTTTGCGCGGCGAAT
GAATCGCACCGCCAAGGCACTGGGAATGACCCGCACGACCTTCAAGAACGCGCATGGGCTGACTGCCAATGGTCACATGT
CGACGGCACGCGACATGACAATCCTCGGACGTCATATGCTTTATGACTACCCTGAGTATTATAATCTCTTTTCGCGCACC
TCGACCAACGCGGGCACCAAGGTTGTCTACAACACCAACCGCCGCTTTCTCGCGGCCTACCGCGGTGCGGATGGCATCAA
GACCGGCTATACGCGCGCAGCCGGGTTCAACCTCGTCTCCTCTGCAAAACGGGGCGATGAGCGCATCATTGCAACCGTAT
TTGGCGGGAAATCCACCGCGGCACGCAACGCCAAGATCGCAGAACTCCTGGACCTCGGCTTCCGGCGCGCTCCGGCCTAT
GCCAAACTGCAAAAGCCCCCTATGCCGCCCTACCTCGGCAACAGCGGTGTGCGCCTGGCAGAGGATGACACGCATGGCAT
GGCCGGAAAGACGATCCGCGTCAGCGGGGTGATTTCAGAGACCCTGCGCCCACGGCCCCGTCCGCTGCCAGAGCCTGCCC
CGGAAACGCTACTTGCAGAGGCTGAAACAGAGGTCGAGACCGGTACTGTTGCCGCACCTGAAGAGGTGCAGCTCGCCGCG
CTGGATAGCGATGCCCTGAAATCCGGCATCAACGACGCTCTTTTGGAAGTCACCGAAGTCTCCGATGTCTCGGCAATCCA
ACCTCAGGCGCGGCCCGAGGCTCTTCAGGCAGAGGTGGAAGGTGAGCAAGCCCTGCAGGCGGAAATCCGAACAGCGGTCG
CGCGCGCCGTTGAGGCCCCGACGCAACCGCGCTACACCGGGCCACGTCCACAGGCGCGCCCAGCAAAATTCAACACCGAA
GTTGCTTCGCTCGAGGAACCAGTCGTGGTGACACGCCTGTCGACCTCTGGCGGCCGCTACTGGGGGATCAATGTCGGACG
CTACACCAACCGTTTTCAGGCCGAAAAAGTCTTGTTGCGCACGGCTTTGGCCGAAATGGAGACGCTCGAAGGCTCTCTGC
GCAAGGTGGTGCAGAGTCCGCGCGGATTTGACGCCAATTTCCTTGGGATGACCAAGGAAAGCGCCGAGCTCGCATGCAAG
CGCCTCAACTCTCGCAATGTCACCTGCGAGACACTTGGCCCCTCCTCCTAA

Upstream 100 bases:

>100_bases
GTTCAGGGCGTTGTGCAGGCGCAGTGCGCAGGCGCTGACCCTCGCACTTGGCGTGGCGTCCATTGCGCCGGAGCGCGCAA
CCGCTGCCCCCTACGCGGCG

Downstream 100 bases:

>100_bases
GCCCTGACGTGCAAGTCAAAAGCCCCGTACCAAAGCCATTGGTGCGGGGGTTTTTCCATTCTTCCAACCAGAAATATCCC
GGGGGTGAATTGCCCGGGAC

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MVMDARTGEVLHSRNADTRLHPASLTKMMTLYIAFEAVRNGEITLDTQITVSRNAEAEPPSELGLRRGQKIALRYLIRAA
AVKSANDAATAIGEAISGSEAAFARRMNRTAKALGMTRTTFKNAHGLTANGHMSTARDMTILGRHMLYDYPEYYNLFSRT
STNAGTKVVYNTNRRFLAAYRGADGIKTGYTRAAGFNLVSSAKRGDERIIATVFGGKSTAARNAKIAELLDLGFRRAPAY
AKLQKPPMPPYLGNSGVRLAEDDTHGMAGKTIRVSGVISETLRPRPRPLPEPAPETLLAEAETEVETGTVAAPEEVQLAA
LDSDALKSGINDALLEVTEVSDVSAIQPQARPEALQAEVEGEQALQAEIRTAVARAVEAPTQPRYTGPRPQARPAKFNTE
VASLEEPVVVTRLSTSGGRYWGINVGRYTNRFQAEKVLLRTALAEMETLEGSLRKVVQSPRGFDANFLGMTKESAELACK
RLNSRNVTCETLGPSS

Sequences:

>Translated_496_residues
MVMDARTGEVLHSRNADTRLHPASLTKMMTLYIAFEAVRNGEITLDTQITVSRNAEAEPPSELGLRRGQKIALRYLIRAA
AVKSANDAATAIGEAISGSEAAFARRMNRTAKALGMTRTTFKNAHGLTANGHMSTARDMTILGRHMLYDYPEYYNLFSRT
STNAGTKVVYNTNRRFLAAYRGADGIKTGYTRAAGFNLVSSAKRGDERIIATVFGGKSTAARNAKIAELLDLGFRRAPAY
AKLQKPPMPPYLGNSGVRLAEDDTHGMAGKTIRVSGVISETLRPRPRPLPEPAPETLLAEAETEVETGTVAAPEEVQLAA
LDSDALKSGINDALLEVTEVSDVSAIQPQARPEALQAEVEGEQALQAEIRTAVARAVEAPTQPRYTGPRPQARPAKFNTE
VASLEEPVVVTRLSTSGGRYWGINVGRYTNRFQAEKVLLRTALAEMETLEGSLRKVVQSPRGFDANFLGMTKESAELACK
RLNSRNVTCETLGPSS
>Mature_496_residues
MVMDARTGEVLHSRNADTRLHPASLTKMMTLYIAFEAVRNGEITLDTQITVSRNAEAEPPSELGLRRGQKIALRYLIRAA
AVKSANDAATAIGEAISGSEAAFARRMNRTAKALGMTRTTFKNAHGLTANGHMSTARDMTILGRHMLYDYPEYYNLFSRT
STNAGTKVVYNTNRRFLAAYRGADGIKTGYTRAAGFNLVSSAKRGDERIIATVFGGKSTAARNAKIAELLDLGFRRAPAY
AKLQKPPMPPYLGNSGVRLAEDDTHGMAGKTIRVSGVISETLRPRPRPLPEPAPETLLAEAETEVETGTVAAPEEVQLAA
LDSDALKSGINDALLEVTEVSDVSAIQPQARPEALQAEVEGEQALQAEIRTAVARAVEAPTQPRYTGPRPQARPAKFNTE
VASLEEPVVVTRLSTSGGRYWGINVGRYTNRFQAEKVLLRTALAEMETLEGSLRKVVQSPRGFDANFLGMTKESAELACK
RLNSRNVTCETLGPSS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=241, Percent_Identity=38.5892116182573, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1786851, Length=241, Percent_Identity=36.5145228215768, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI87082022, Length=239, Percent_Identity=35.9832635983264, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87082054, Length=213, Percent_Identity=29.5774647887324, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53739; Mature: 53739

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMDARTGEVLHSRNADTRLHPASLTKMMTLYIAFEAVRNGEITLDTQITVSRNAEAEPP
CCCCCCCCHHHHCCCCCCEECHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
SELGLRRGQKIALRYLIRAAAVKSANDAATAIGEAISGSEAAFARRMNRTAKALGMTRTT
HHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHH
FKNAHGLTANGHMSTARDMTILGRHMLYDYPEYYNLFSRTSTNAGTKVVYNTNRRFLAAY
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCEEEEEE
RGADGIKTGYTRAAGFNLVSSAKRGDERIIATVFGGKSTAARNAKIAELLDLGFRRAPAY
CCCCCCCCCCCHHHCCHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCH
AKLQKPPMPPYLGNSGVRLAEDDTHGMAGKTIRVSGVISETLRPRPRPLPEPAPETLLAE
HHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCHHHHHH
AETEVETGTVAAPEEVQLAALDSDALKSGINDALLEVTEVSDVSAIQPQARPEALQAEVE
HHCCCCCCCCCCCCCEEEEECCHHHHHCCCCHHHHHHHHCCCCHHCCCCCCHHHHHHHCC
GEQALQAEIRTAVARAVEAPTQPRYTGPRPQARPAKFNTEVASLEEPVVVTRLSTSGGRY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCEE
WGINVGRYTNRFQAEKVLLRTALAEMETLEGSLRKVVQSPRGFDANFLGMTKESAELACK
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
RLNSRNVTCETLGPSS
HHCCCCCEEECCCCCC
>Mature Secondary Structure
MVMDARTGEVLHSRNADTRLHPASLTKMMTLYIAFEAVRNGEITLDTQITVSRNAEAEPP
CCCCCCCCHHHHCCCCCCEECHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
SELGLRRGQKIALRYLIRAAAVKSANDAATAIGEAISGSEAAFARRMNRTAKALGMTRTT
HHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHH
FKNAHGLTANGHMSTARDMTILGRHMLYDYPEYYNLFSRTSTNAGTKVVYNTNRRFLAAY
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCEEEEEE
RGADGIKTGYTRAAGFNLVSSAKRGDERIIATVFGGKSTAARNAKIAELLDLGFRRAPAY
CCCCCCCCCCCHHHCCHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCH
AKLQKPPMPPYLGNSGVRLAEDDTHGMAGKTIRVSGVISETLRPRPRPLPEPAPETLLAE
HHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCHHHHHH
AETEVETGTVAAPEEVQLAALDSDALKSGINDALLEVTEVSDVSAIQPQARPEALQAEVE
HHCCCCCCCCCCCCCEEEEECCHHHHHCCCCHHHHHHHHCCCCHHCCCCCCHHHHHHHCC
GEQALQAEIRTAVARAVEAPTQPRYTGPRPQARPAKFNTEVASLEEPVVVTRLSTSGGRY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCEE
WGINVGRYTNRFQAEKVLLRTALAEMETLEGSLRKVVQSPRGFDANFLGMTKESAELACK
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
RLNSRNVTCETLGPSS
HHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]