Click here to switch to the map view.

The map label for this gene is leuC

Identifier: 99082348

GI number: 99082348

Start: 2649508

End: 2650911

Strand: Direct

Name: leuC

Synonym: TM1040_2508

Alternate gene names: 99082348

Gene position: 2649508-2650911 (Clockwise)

Preceding gene: 99082344

Following gene: 99082349

Centisome position: 82.77

GC content: 62.89

Gene sequence:

>1404_bases
ATGTCCCCCAAAACACTCTATGACAAGATCTGGGATGCGCATGTCGCGCAAGAAGCCGAAGACGGCACCTGTCTGCTTTA
TATCGACCGTCACCTCGTTCACGAGGTGACAAGCCCGCAAGCCTTTGAAGGGCTGCGCATGGCAGGTCGCAAGGTGCACG
CGCCAGAGAAAACCATCGCGGTGCCAGATCACAACGTCCCCACCACTGCAGGACGCGAAAACCCCGACCAGATGCCGGAG
GACAGCCGCATCCAGGTAGCCGCCCTTGACACCAACGCGCGTGAGTTCGGCGTGCATTACTATCCGGTGACCGACATCCG
CCAGGGTATCGTGCACATCGTCGGCCCCGAGCAAGGCTGGACCCTGCCCGGTATGACCGTGGTCTGTGGCGACAGCCACA
CCGCGACCCACGGTGCCTTTGGCGCGCTGGCGCATGGCATTGGCACCTCGGAGGTGGAGCATGTGCTTGCCACCCAGACG
CTGATCCAGAAGAAGTCCAAGAACATGAAGGTCGAGATCACCGGCAAGCTGTCGCCGGGCGTGACCGCCAAGGACATCGT
TCTGACCATCATTGGCGAAACCGGCACCGGTGGCGGCACTGGCTATGTCATCGAGTATTGCGGTGAAGCGATCCGCGATC
TGTCGATGGAAGGCCGCATGACAATCTGCAACATGGCCATTGAGGGCGGCGCACGCGCCGGCCTGATCGCGCCCGACGAG
ACCACCTTTGAATACGTCAAAGGCCGCCCGCATGCCCCCAAAGGCGCGCAGTGGGAAGCCGCCGTGAACTGGTGGAAAAC
GCTCTACTCTGACGACGACGCCCATTGGGACAAGATTGTGACCATCCGCGGCGAAGACATCGCGCCGACCGTCACATGGG
GCACCAGCCCCGAAGACGCGCTGCCGATCACCGCAACCGTCCCCGCCCCCGAGGATTTCACTGGTGGCAAAGTCGAGGCC
GCGCGCCGCGCGCTTGACTACATGGGCCTCACCCCCGGGATGAAGCTGTCGGACATCGAGATCGACACCGTGTTCATCGG
CTCCTGCACCAACGGCCGCATCGAAGATCTGCGCGCCGCTGCGGATGTAGTCAAAGGCAAGAAGATCAAGGACGGCATGC
GCGCCATGGTGGTTCCGGGTTCCGGCCTCGTGCGCGCCCAGGCAGAAGAAGAGGGCCTTGCCGAGATCTTCAAGGATGCC
GGTTTCGAATGGCGTCTGGCGGGCTGCTCCATGTGTCTGGCAATGAACCCCGACCAGCTGAGCGAGGGCGAGCGCTGCGC
CTCCACCTCCAACCGGAACTTTGAAGGCCGTCAGGGCTTTAAGGGACGCACGCACCTGGTGTCGCCAGCCATGGCCGCCG
CTGCTGCCGTTACCGGCAAACTCACCGACGTTCGCGAGCTCTGA

Upstream 100 bases:

>100_bases
AACGGTTCTTTGGTGCCGCCCGCACGGTTCTTGCGCGCCTATAAGCTGCGCAGCCAGACCAGTTTCGCCAGCACATTTAC
CCACATACGGGAGAGGACCG

Downstream 100 bases:

>100_bases
AGGACAACACCCATGGAAAAGTTCGAAACCTTCACCGGGATTGCGGCGCCAATGCCGCTGGTCAACATCGACACCGATAT
GATCATCCCCAAGCAGTTCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSPKTLYDKIWDAHVAQEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRKVHAPEKTIAVPDHNVPTTAGRENPDQMPE
DSRIQVAALDTNAREFGVHYYPVTDIRQGIVHIVGPEQGWTLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQT
LIQKKSKNMKVEITGKLSPGVTAKDIVLTIIGETGTGGGTGYVIEYCGEAIRDLSMEGRMTICNMAIEGGARAGLIAPDE
TTFEYVKGRPHAPKGAQWEAAVNWWKTLYSDDDAHWDKIVTIRGEDIAPTVTWGTSPEDALPITATVPAPEDFTGGKVEA
ARRALDYMGLTPGMKLSDIEIDTVFIGSCTNGRIEDLRAAADVVKGKKIKDGMRAMVVPGSGLVRAQAEEEGLAEIFKDA
GFEWRLAGCSMCLAMNPDQLSEGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAVTGKLTDVREL

Sequences:

>Translated_467_residues
MSPKTLYDKIWDAHVAQEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRKVHAPEKTIAVPDHNVPTTAGRENPDQMPE
DSRIQVAALDTNAREFGVHYYPVTDIRQGIVHIVGPEQGWTLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQT
LIQKKSKNMKVEITGKLSPGVTAKDIVLTIIGETGTGGGTGYVIEYCGEAIRDLSMEGRMTICNMAIEGGARAGLIAPDE
TTFEYVKGRPHAPKGAQWEAAVNWWKTLYSDDDAHWDKIVTIRGEDIAPTVTWGTSPEDALPITATVPAPEDFTGGKVEA
ARRALDYMGLTPGMKLSDIEIDTVFIGSCTNGRIEDLRAAADVVKGKKIKDGMRAMVVPGSGLVRAQAEEEGLAEIFKDA
GFEWRLAGCSMCLAMNPDQLSEGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAVTGKLTDVREL
>Mature_466_residues
SPKTLYDKIWDAHVAQEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRKVHAPEKTIAVPDHNVPTTAGRENPDQMPED
SRIQVAALDTNAREFGVHYYPVTDIRQGIVHIVGPEQGWTLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQTL
IQKKSKNMKVEITGKLSPGVTAKDIVLTIIGETGTGGGTGYVIEYCGEAIRDLSMEGRMTICNMAIEGGARAGLIAPDET
TFEYVKGRPHAPKGAQWEAAVNWWKTLYSDDDAHWDKIVTIRGEDIAPTVTWGTSPEDALPITATVPAPEDFTGGKVEAA
RRALDYMGLTPGMKLSDIEIDTVFIGSCTNGRIEDLRAAADVVKGKKIKDGMRAMVVPGSGLVRAQAEEEGLAEIFKDAG
FEWRLAGCSMCLAMNPDQLSEGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAVTGKLTDVREL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=477, Percent_Identity=27.8825995807128, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI8659555, Length=439, Percent_Identity=24.373576309795, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI41352693, Length=385, Percent_Identity=25.974025974026, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=65.6652360515021, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.0387811634349, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081781, Length=356, Percent_Identity=25, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI2367097, Length=351, Percent_Identity=25.6410256410256, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=477, Percent_Identity=28.0922431865828, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=384, Percent_Identity=28.90625, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=29.0849673202614, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=375, Percent_Identity=26.9333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=64.1949152542373, Blast_Score=612, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6320440, Length=430, Percent_Identity=26.5116279069767, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=470, Percent_Identity=27.6595744680851, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=382, Percent_Identity=28.7958115183246, Blast_Score=143, Evalue=6e-35,
Organism=Drosophila melanogaster, GI281365315, Length=474, Percent_Identity=26.5822784810127, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17864292, Length=474, Percent_Identity=26.5822784810127, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161076999, Length=361, Percent_Identity=28.2548476454294, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28571643, Length=474, Percent_Identity=25.5274261603376, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=28.1081081081081, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17137564, Length=372, Percent_Identity=27.6881720430108, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SILST (Q1GDM6)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614502.1
- ProteinModelPortal:   Q1GDM6
- SMR:   Q1GDM6
- STRING:   Q1GDM6
- GeneID:   4076510
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2508
- NMPDR:   fig|292414.1.peg.773
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q1GDM6
- ProtClustDB:   PRK05478
- BioCyc:   SSP292414:TM1040_2508-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50265; Mature: 50134

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKTLYDKIWDAHVAQEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRKVHAPEKTIA
CCCHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCEECCCCCEEE
VPDHNVPTTAGRENPDQMPEDSRIQVAALDTNAREFGVHYYPVTDIRQGIVHIVGPEQGW
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHCEEEEEHHHHCCCEEEEECCCCCC
TLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQTLIQKKSKNMKVEITGKLSPG
CCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
VTAKDIVLTIIGETGTGGGTGYVIEYCGEAIRDLSMEGRMTICNMAIEGGARAGLIAPDE
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCC
TTFEYVKGRPHAPKGAQWEAAVNWWKTLYSDDDAHWDKIVTIRGEDIAPTVTWGTSPEDA
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCCCCCC
LPITATVPAPEDFTGGKVEAARRALDYMGLTPGMKLSDIEIDTVFIGSCTNGRIEDLRAA
CCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECEEEEEEEEECCCCCHHHHHHHH
ADVVKGKKIKDGMRAMVVPGSGLVRAQAEEEGLAEIFKDAGFEWRLAGCSMCLAMNPDQL
HHHHCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHCCCEEEEECCEEEEEECCHHC
SEGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAVTGKLTDVREL
CCCCHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
SPKTLYDKIWDAHVAQEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRKVHAPEKTIA
CCHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCEECCCCCEEE
VPDHNVPTTAGRENPDQMPEDSRIQVAALDTNAREFGVHYYPVTDIRQGIVHIVGPEQGW
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHCEEEEEHHHHCCCEEEEECCCCCC
TLPGMTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQTLIQKKSKNMKVEITGKLSPG
CCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
VTAKDIVLTIIGETGTGGGTGYVIEYCGEAIRDLSMEGRMTICNMAIEGGARAGLIAPDE
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCC
TTFEYVKGRPHAPKGAQWEAAVNWWKTLYSDDDAHWDKIVTIRGEDIAPTVTWGTSPEDA
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCCCCCC
LPITATVPAPEDFTGGKVEAARRALDYMGLTPGMKLSDIEIDTVFIGSCTNGRIEDLRAA
CCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECEEEEEEEEECCCCCHHHHHHHH
ADVVKGKKIKDGMRAMVVPGSGLVRAQAEEEGLAEIFKDAGFEWRLAGCSMCLAMNPDQL
HHHHCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHCCCEEEEECCEEEEEECCHHC
SEGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAVTGKLTDVREL
CCCCHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA