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The map label for this gene is camA [H]

Identifier: 99082311

GI number: 99082311

Start: 2611032

End: 2612243

Strand: Reverse

Name: camA [H]

Synonym: TM1040_2471

Alternate gene names: 99082311

Gene position: 2612243-2611032 (Counterclockwise)

Preceding gene: 99082314

Following gene: 99082310

Centisome position: 81.61

GC content: 58.83

Gene sequence:

>1212_bases
ATGACTCATATCGCTGTGATTGGCGCAGGTCAGGCAGGGTCCTCCCTGGTTGCCAAGCTGCGCAAATGCGGATTTGACGG
TGAGATCACCCTGATCGGTGCCGAGAAGGTGCTCCCGTATCAACGCCCTCCGCTGTCCAAGGCCTATTTGCTGGGTGAAA
TGGAACTTGAGCGCCTGTTTCTGCGGCCCGAGAGTTTTTATGCCGAGAACAACATCACCCTGCGTCTTGGTACCAAGGTG
GATGCGATCGACGCGGCTGCAAAGACTTTGCAGATCGGCGACGAAACGCTGTCCTATGATCAGCTTGTGCTCACGACTGG
GTCGCACCCACGTCACCTGCCTGCTGCCATCGGTGGCGATTTGGGCGGTGTGCATGTTGTGCGCGACCTTAAGGATGTAG
ACGCGATGGCCCCCGCGGTTACGGATGGCGCGCGCGCATTGATTGTCGGCGGCGGTTATATCGGCCTCGAGGCGGCCGCG
GTCTGCGCCAAACGGGGTGTATCAGTCACGTTGGTTGAAATGGCGGACCGCATTTTGCAACGAGTCGCCGCGCCAGAGAC
TTCCGATTACTTCCGCGCGCTGCACAGCGCGCAAGGTGTAGACATCCGCGAGGGCGTGGGCCTGTCGCATCTGGAGGGAG
ACGCAGGCAAGGTGACCTGCGCGGTGCTGGCGGATGGCACCCGTCTCGATGTGGATTTTGTGGTCGTTGGTGTCGGGATC
ACCCCAGCCTCTGAACTGGCCGCAGACGCGGGGCTCGAGATCGAAAACGGCATTCGCACGGATGAGCTGGGCCGCACCTC
GGATCCGGCAATCTGGGCCGCAGGCGATTGCGCATCCTTTCCGTATAAGGGCCAGCGAATCCGCTTGGAAAGCGTGCCAA
ACGCAATTGATCAGGCCGAAGTGGTGGCGGAAAATCTCCTAGGGGCAGAAAAAGCCTATGTGGCTACGCCCTGGTTCTGG
TCGGATCAATACGATGTGAAACTGCAAATTGCGGGTCTGAATTCAGGCTATGACAATGTTGTGACGCGTCAGGGCGCAGA
TGGCTCCATGTCCTTCTGGTATTACACCGGGGATCAACTGGTGGCTGTGGATGCGATGAATGATCCACGCGCTTACATGG
TAGCAAAACGCTTGATCGAAGCCGGAAAAACGGCGGACAAGGCGATTGTGGTCGATCCCGAAGCGGATCTGAAGCCGCTC
CTCAAGGCATGA

Upstream 100 bases:

>100_bases
TGGGGCGCCCTGGCGATTTTGTGCAATTGCATCCCGGCGCGCCCTCCATGTAGGGTTCGCTCGAACGGTATATCAACTGC
ATCAGGAGACAAGAGCCCAG

Downstream 100 bases:

>100_bases
GGATCATTGCCGGCGCGTTTCGGGGCCGCGCCCTCGCCTCCGTGGGCAAGGGCGATGCGGGCGCGCATTTGCGCCCGACC
ACCGACCGGGTCCGCGAAAG

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTHIAVIGAGQAGSSLVAKLRKCGFDGEITLIGAEKVLPYQRPPLSKAYLLGEMELERLFLRPESFYAENNITLRLGTKV
DAIDAAAKTLQIGDETLSYDQLVLTTGSHPRHLPAAIGGDLGGVHVVRDLKDVDAMAPAVTDGARALIVGGGYIGLEAAA
VCAKRGVSVTLVEMADRILQRVAAPETSDYFRALHSAQGVDIREGVGLSHLEGDAGKVTCAVLADGTRLDVDFVVVGVGI
TPASELAADAGLEIENGIRTDELGRTSDPAIWAAGDCASFPYKGQRIRLESVPNAIDQAEVVAENLLGAEKAYVATPWFW
SDQYDVKLQIAGLNSGYDNVVTRQGADGSMSFWYYTGDQLVAVDAMNDPRAYMVAKRLIEAGKTADKAIVVDPEADLKPL
LKA

Sequences:

>Translated_403_residues
MTHIAVIGAGQAGSSLVAKLRKCGFDGEITLIGAEKVLPYQRPPLSKAYLLGEMELERLFLRPESFYAENNITLRLGTKV
DAIDAAAKTLQIGDETLSYDQLVLTTGSHPRHLPAAIGGDLGGVHVVRDLKDVDAMAPAVTDGARALIVGGGYIGLEAAA
VCAKRGVSVTLVEMADRILQRVAAPETSDYFRALHSAQGVDIREGVGLSHLEGDAGKVTCAVLADGTRLDVDFVVVGVGI
TPASELAADAGLEIENGIRTDELGRTSDPAIWAAGDCASFPYKGQRIRLESVPNAIDQAEVVAENLLGAEKAYVATPWFW
SDQYDVKLQIAGLNSGYDNVVTRQGADGSMSFWYYTGDQLVAVDAMNDPRAYMVAKRLIEAGKTADKAIVVDPEADLKPL
LKA
>Mature_402_residues
THIAVIGAGQAGSSLVAKLRKCGFDGEITLIGAEKVLPYQRPPLSKAYLLGEMELERLFLRPESFYAENNITLRLGTKVD
AIDAAAKTLQIGDETLSYDQLVLTTGSHPRHLPAAIGGDLGGVHVVRDLKDVDAMAPAVTDGARALIVGGGYIGLEAAAV
CAKRGVSVTLVEMADRILQRVAAPETSDYFRALHSAQGVDIREGVGLSHLEGDAGKVTCAVLADGTRLDVDFVVVGVGIT
PASELAADAGLEIENGIRTDELGRTSDPAIWAAGDCASFPYKGQRIRLESVPNAIDQAEVVAENLLGAEKAYVATPWFWS
DQYDVKLQIAGLNSGYDNVVTRQGADGSMSFWYYTGDQLVAVDAMNDPRAYMVAKRLIEAGKTADKAIVVDPEADLKPLL
KA

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=386, Percent_Identity=29.2746113989637, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI226437568, Length=386, Percent_Identity=29.2746113989637, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI65787454, Length=386, Percent_Identity=29.2746113989637, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4757732, Length=333, Percent_Identity=26.4264264264264, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI22202629, Length=333, Percent_Identity=26.4264264264264, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1788892, Length=404, Percent_Identity=33.6633663366337, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789065, Length=311, Percent_Identity=32.1543408360129, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789765, Length=317, Percent_Identity=29.0220820189274, Blast_Score=98, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=371, Percent_Identity=26.1455525606469, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24639257, Length=402, Percent_Identity=29.1044776119403, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI281359715, Length=402, Percent_Identity=29.1044776119403, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI281359713, Length=402, Percent_Identity=29.1044776119403, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24639250, Length=402, Percent_Identity=29.1044776119403, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI18543267, Length=402, Percent_Identity=29.1044776119403, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24639252, Length=402, Percent_Identity=29.1044776119403, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24585130, Length=411, Percent_Identity=26.0340632603406, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=28.2913165266106, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=28.2913165266106, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42796; Mature: 42665

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHIAVIGAGQAGSSLVAKLRKCGFDGEITLIGAEKVLPYQRPPLSKAYLLGEMELERLF
CCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCHHEEECHHHHHHHH
LRPESFYAENNITLRLGTKVDAIDAAAKTLQIGDETLSYDQLVLTTGSHPRHLPAAIGGD
CCCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHCCC
LGGVHVVRDLKDVDAMAPAVTDGARALIVGGGYIGLEAAAVCAKRGVSVTLVEMADRILQ
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEHHHHHHHHHH
RVAAPETSDYFRALHSAQGVDIREGVGLSHLEGDAGKVTCAVLADGTRLDVDFVVVGVGI
HHHCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEEEECC
TPASELAADAGLEIENGIRTDELGRTSDPAIWAAGDCASFPYKGQRIRLESVPNAIDQAE
CCHHHHHHHCCCEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEHHCCCHHHHHH
VVAENLLGAEKAYVATPWFWSDQYDVKLQIAGLNSGYDNVVTRQGADGSMSFWYYTGDQL
HHHHHHCCCCCCEEECCEEECCCEEEEEEEEECCCCCCHHEEECCCCCCEEEEEEECCEE
VAVDAMNDPRAYMVAKRLIEAGKTADKAIVVDPEADLKPLLKA
EEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHCC
>Mature Secondary Structure 
THIAVIGAGQAGSSLVAKLRKCGFDGEITLIGAEKVLPYQRPPLSKAYLLGEMELERLF
CEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCHHEEECHHHHHHHH
LRPESFYAENNITLRLGTKVDAIDAAAKTLQIGDETLSYDQLVLTTGSHPRHLPAAIGGD
CCCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHCCC
LGGVHVVRDLKDVDAMAPAVTDGARALIVGGGYIGLEAAAVCAKRGVSVTLVEMADRILQ
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEHHHHHHHHHH
RVAAPETSDYFRALHSAQGVDIREGVGLSHLEGDAGKVTCAVLADGTRLDVDFVVVGVGI
HHHCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEEEECC
TPASELAADAGLEIENGIRTDELGRTSDPAIWAAGDCASFPYKGQRIRLESVPNAIDQAE
CCHHHHHHHCCCEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEHHCCCHHHHHH
VVAENLLGAEKAYVATPWFWSDQYDVKLQIAGLNSGYDNVVTRQGADGSMSFWYYTGDQL
HHHHHHCCCCCCEEECCEEECCCEEEEEEEEECCCCCCHHEEECCCCCCEEEEEEECCEE
VAVDAMNDPRAYMVAKRLIEAGKTADKAIVVDPEADLKPLLKA
EEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]