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The map label for this gene is gcvH [H]

Identifier: 99082232

GI number: 99082232

Start: 2515630

End: 2515989

Strand: Reverse

Name: gcvH [H]

Synonym: TM1040_2392

Alternate gene names: 99082232

Gene position: 2515989-2515630 (Counterclockwise)

Preceding gene: 99082233

Following gene: 99082231

Centisome position: 78.6

GC content: 56.94

Gene sequence:

>360_bases
ATGAAATACACCGAAGAACACGAATGGCTGCAGGTCGAAGGCGACGTTGTGACTGTTGGCATCACCTCCCACGCGGCCGA
GCAGCTGGGCGATGTGGTCTTTGTTGAGCTTCCCGAAGTGGGCAGCGAAGTCTCCAAGGACGACGAGATCGTGGTCATCG
AATCCGTTAAGGCAGCGTCGGACATTCTCGCGCCGCTCGACGGTGAAGTGGTCGAAGTCAACGAGGCCATCGTGGACGCG
CCGTCCAAGGTCAATGAGGACCCGGAAGGCGAAGCTTGGTTCTTCAAACTCAAAGTCTCCGACATGTCGCCGATGGACGA
CTACATGGACGAGGCTGGCTACAAAGACTTCATTGGCTGA

Upstream 100 bases:

>100_bases
AGCGCGTGCCCGTCACTGTTGCAAAGCTGCCCTTCGTGGCGGCCAACTTCAAACGCTAAAACCGGTCCCCGCTAAAGCGG
CCAACACAGGAAGAGAACCC

Downstream 100 bases:

>100_bases
TGCGTTTGGGCCCGTTGCCGTGTGGTGCGGGCCCCTCTTTTACCTGCAAGGGCGTAACCGGGTTTGCCCGACCCAATGGT
GCACGCCTTTGATCCCGAGA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MKYTEEHEWLQVEGDVVTVGITSHAAEQLGDVVFVELPEVGSEVSKDDEIVVIESVKAASDILAPLDGEVVEVNEAIVDA
PSKVNEDPEGEAWFFKLKVSDMSPMDDYMDEAGYKDFIG

Sequences:

>Translated_119_residues
MKYTEEHEWLQVEGDVVTVGITSHAAEQLGDVVFVELPEVGSEVSKDDEIVVIESVKAASDILAPLDGEVVEVNEAIVDA
PSKVNEDPEGEAWFFKLKVSDMSPMDDYMDEAGYKDFIG
>Mature_119_residues
MKYTEEHEWLQVEGDVVTVGITSHAAEQLGDVVFVELPEVGSEVSKDDEIVVIESVKAASDILAPLDGEVVEVNEAIVDA
PSKVNEDPEGEAWFFKLKVSDMSPMDDYMDEAGYKDFIG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=46.1538461538462, Blast_Score=118, Evalue=9e-28,
Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=46.1538461538462, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=52.9411764705882, Blast_Score=127, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=45.2173913043478, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=46.9565217391304, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=102, Percent_Identity=47.0588235294118, Blast_Score=100, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=43.2203389830509, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13065; Mature: 13065

Theoretical pI: Translated: 3.67; Mature: 3.67

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTEEHEWLQVEGDVVTVGITSHAAEQLGDVVFVELPEVGSEVSKDDEIVVIESVKAAS
CCCCCCCCEEEECCCEEEEEECHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEECCCCHH
DILAPLDGEVVEVNEAIVDAPSKVNEDPEGEAWFFKLKVSDMSPMDDYMDEAGYKDFIG
HHCCCCCCCEEEECHHHHCCCHHCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKYTEEHEWLQVEGDVVTVGITSHAAEQLGDVVFVELPEVGSEVSKDDEIVVIESVKAAS
CCCCCCCCEEEECCCEEEEEECHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEECCCCHH
DILAPLDGEVVEVNEAIVDAPSKVNEDPEGEAWFFKLKVSDMSPMDDYMDEAGYKDFIG
HHCCCCCCCEEEECHHHHCCCHHCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]