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The map label for this gene is ruvA [H]

Identifier: 99082212

GI number: 99082212

Start: 2492868

End: 2493557

Strand: Reverse

Name: ruvA [H]

Synonym: TM1040_2372

Alternate gene names: 99082212

Gene position: 2493557-2492868 (Counterclockwise)

Preceding gene: 99082213

Following gene: 99082211

Centisome position: 77.9

GC content: 63.62

Gene sequence:

>690_bases
ATGATCGGCAAATTGACCGGGCGTCTGGATTACAAGACTCCGGACCATGTGATGATCGACGTGCGCGGCGTCGGTTATAT
CGTCTACTGCTCGGATCGCACCTTGGCGAGCCTGCCTGCTGTAGGCGAGGCGGTTCAGCTTTATACCGAGCTTTTGGTGC
GCGAAGATCTCATGCAGCTCTTTGGGTTCACGTCCTTGGTGGAAAAGGAATGGCACCGATTGCTGACCTCGGTGCAGGGG
GTAGGGGCCAAAGTGTCGCTTGCCATCCTGAGTGCGCTGGGGCCCGAAGGTGTCAGCCGTGCGATCGCCCTTGGCGATTG
GGCGGCGGTGAAAGCCGCCAAAGGGGTTGGCCCCAAGACCGCGCAGCGCATCGTGCTCGACCTTAAAGATAAGGCACCCT
CCGTGATGGCGATGGGGGGCACGGTTACAGAGGCGCTAGAGGGGCCGGAATTGGACGCAGTGGTCGAACCCGCAGCTGAG
GCGCCGGCAAAGCCTGCGCGCAAAGCCGCCCCAAAGGCCCCCACCGGCGCGGCAGCCGCTTCGGCGGGGGCACTGTCGGC
ATTGGGCAACCTGGGTTACGGGCCTTCCGATGCCGCAGGTGCGGTCGCAGAGGCCGCCGCCAACAATCCAGACGCAGGCG
AAGCGGAATTGATCCGTGCCGCGCTTCGGCTCTTGGCACCCAAAGGGTGA

Upstream 100 bases:

>100_bases
GTGCGCCCAGGGGCGCGGATGCGGCTGATGCTCTGGCGATTGCGATCTGTCACGCCTATTACGGAGGCAGCAACCAGCGG
CAACTCAAAGAGGTGGGCGC

Downstream 100 bases:

>100_bases
GGACGCTTTGAATGATTGATGCAGACCCCACCCTGCGCCCGGAGCCTCTGCCCGAAGACAATGATCGCGCCCTGCGCCCG
CAGGGCCTGGGTGAGTTTAT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MIGKLTGRLDYKTPDHVMIDVRGVGYIVYCSDRTLASLPAVGEAVQLYTELLVREDLMQLFGFTSLVEKEWHRLLTSVQG
VGAKVSLAILSALGPEGVSRAIALGDWAAVKAAKGVGPKTAQRIVLDLKDKAPSVMAMGGTVTEALEGPELDAVVEPAAE
APAKPARKAAPKAPTGAAAASAGALSALGNLGYGPSDAAGAVAEAAANNPDAGEAELIRAALRLLAPKG

Sequences:

>Translated_229_residues
MIGKLTGRLDYKTPDHVMIDVRGVGYIVYCSDRTLASLPAVGEAVQLYTELLVREDLMQLFGFTSLVEKEWHRLLTSVQG
VGAKVSLAILSALGPEGVSRAIALGDWAAVKAAKGVGPKTAQRIVLDLKDKAPSVMAMGGTVTEALEGPELDAVVEPAAE
APAKPARKAAPKAPTGAAAASAGALSALGNLGYGPSDAAGAVAEAAANNPDAGEAELIRAALRLLAPKG
>Mature_229_residues
MIGKLTGRLDYKTPDHVMIDVRGVGYIVYCSDRTLASLPAVGEAVQLYTELLVREDLMQLFGFTSLVEKEWHRLLTSVQG
VGAKVSLAILSALGPEGVSRAIALGDWAAVKAAKGVGPKTAQRIVLDLKDKAPSVMAMGGTVTEALEGPELDAVVEPAAE
APAKPARKAAPKAPTGAAAASAGALSALGNLGYGPSDAAGAVAEAAANNPDAGEAELIRAALRLLAPKG

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family [H]

Homologues:

Organism=Escherichia coli, GI1788168, Length=223, Percent_Identity=32.7354260089686, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994 [H]

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 23346; Mature: 23346

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKLTGRLDYKTPDHVMIDVRGVGYIVYCSDRTLASLPAVGEAVQLYTELLVREDLMQL
CCCCCCCCCCCCCCCEEEEEEECCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
FGFTSLVEKEWHRLLTSVQGVGAKVSLAILSALGPEGVSRAIALGDWAAVKAAKGVGPKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHCCCCHHH
AQRIVLDLKDKAPSVMAMGGTVTEALEGPELDAVVEPAAEAPAKPARKAAPKAPTGAAAA
HHHHHHHHCCCCCCEEEECCHHHHHHCCCCCHHHHCCHHCCCCCCHHHCCCCCCCCCHHH
SAGALSALGNLGYGPSDAAGAVAEAAANNPDAGEAELIRAALRLLAPKG
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIGKLTGRLDYKTPDHVMIDVRGVGYIVYCSDRTLASLPAVGEAVQLYTELLVREDLMQL
CCCCCCCCCCCCCCCEEEEEEECCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
FGFTSLVEKEWHRLLTSVQGVGAKVSLAILSALGPEGVSRAIALGDWAAVKAAKGVGPKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHCCCCHHH
AQRIVLDLKDKAPSVMAMGGTVTEALEGPELDAVVEPAAEAPAKPARKAAPKAPTGAAAA
HHHHHHHHCCCCCCEEEECCHHHHHHCCCCCHHHHCCHHCCCCCCHHHCCCCCCCCCHHH
SAGALSALGNLGYGPSDAAGAVAEAAANNPDAGEAELIRAALRLLAPKG
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA