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The map label for this gene is tolB

Identifier: 99082205

GI number: 99082205

Start: 2486763

End: 2488088

Strand: Reverse

Name: tolB

Synonym: TM1040_2365

Alternate gene names: 99082205

Gene position: 2488088-2486763 (Counterclockwise)

Preceding gene: 99082206

Following gene: 99082204

Centisome position: 77.73

GC content: 61.09

Gene sequence:

>1326_bases
ATGAGAAACGCAATTGCGACAGTACTCCTTGGCCTTGCAATGCTGCTGCCCGTTGGGGCGGTGCAGGCACAGGATGGGCC
GCTCCGGATCGAGATCACGGATGGTGTGATCGAACCGTTGCCCTTCGCGGTGCCCGATTTCATCGCCGACACCCCGGCAG
CGGCGCAGTATGCGACGGACATTGCCCGTGTGATCGCCCAGGATCTTGTGGGCTCCGGGCTGTTTCGCGAAATTCCCAAG
GTCGCCTATATTTCGCCCTACACCGATTTTGACGCTGAGGTGAATTTCACCGACTGGAAGGCGATCAACGCGCAGGCGTT
GATTGCAGGCGCGGTGAGCCTGTCGGGCGATCGCATCACCGTGCGCTTTCGCGGCTATGACGTGTTCGCCGGGCAAGAGC
TTGGCGCGGGGCTTCAGCTTTCGGGCACGACCGACGGCTGGCGGCGTATCGCGCATAAGGTGGCCGATACGATCTATTCT
CGCCTGACCGGCGAAGGTGGCTATTTTGACAGCCGCGTCGTGTTTGTTTCGGAAACCGGCCCCAAGAATGACCGCAAGAA
GCGCCTTGCGATCATGGATTACGATGGGGCCAATGTGCAGTATCTGACCAACTCGGACGCCATTGTGCTCGCGCCCCGGT
TCTCGCCCACCGGCGACCGCGTGCTTTATACCAGCTATGAGAGCGGCTTTCCGCAAATCCATGTGCTGGATGTCGGCCGC
GTGCAGCGGCGGGTATTGTCGGCGGGCAATGGCTCCATGAGCTTTGCGCCGCGTTTTGCCCCCAACGGCCAGACGATTGT
CTACTCGCAGTCTCAAGGCGGCAATACCGACCTCTTTTCCATGGACATCAACTCTGGCAACCCGCAGCGCCTGACCAGTG
CGCCGTCGATTGAAACCGCCCCGTCATTCTCGCCCGATGGCAGCCAGATCGTGTTTGAGAGCGACCGCTCCGGGACATCG
CAGCTCTATGTGATGTCTGCGTCGGGTGGAGAGGCCAAGCGGATCAGTTTTGGTCAGGGGCGCTATGGCACCCCAGTCTG
GTCGCCGCGTGGCGACTACATCGCCTTCACCAAGCAGAACGCGGGCCGGTTTCATATTGGCGTGATGCGCACAGATGGTA
GCGAGGAACGCCTGCTGACGGCGTCCTTTCTTGACGAGGGTCCCACATGGTCGCCCAATGGTCGGGTGATCATGTTCACG
CGCGAAACCCAGGGGGCCAGCGGTCAGGCGCGGCTCTATTCGGTGGATATTTCAGGTCGCAATCTGAAGCCGGTCAAGAC
GCCGGATGGGGCGTCTGACCCCTCCTGGTCCCCTCTGCAGAACTGA

Upstream 100 bases:

>100_bases
TCATCCGCTGTGGATCAAGAGGATTTGATCTTCCGGCTGACAAATACGCTCAGTGGCGTGATATTGAGATGACATTTAAC
CCCGAAGGAATGCGGATCAG

Downstream 100 bases:

>100_bases
TGAACCGCCCGGCCAAGAACCACGCGTGCAAGCCCGTGGCGTGGCCGGGTACAATATGCTAACACTTCGGTAAGAAATGT
CCGAGCACACGAGGCGATTT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MRNAIATVLLGLAMLLPVGAVQAQDGPLRIEITDGVIEPLPFAVPDFIADTPAAAQYATDIARVIAQDLVGSGLFREIPK
VAYISPYTDFDAEVNFTDWKAINAQALIAGAVSLSGDRITVRFRGYDVFAGQELGAGLQLSGTTDGWRRIAHKVADTIYS
RLTGEGGYFDSRVVFVSETGPKNDRKKRLAIMDYDGANVQYLTNSDAIVLAPRFSPTGDRVLYTSYESGFPQIHVLDVGR
VQRRVLSAGNGSMSFAPRFAPNGQTIVYSQSQGGNTDLFSMDINSGNPQRLTSAPSIETAPSFSPDGSQIVFESDRSGTS
QLYVMSASGGEAKRISFGQGRYGTPVWSPRGDYIAFTKQNAGRFHIGVMRTDGSEERLLTASFLDEGPTWSPNGRVIMFT
RETQGASGQARLYSVDISGRNLKPVKTPDGASDPSWSPLQN

Sequences:

>Translated_441_residues
MRNAIATVLLGLAMLLPVGAVQAQDGPLRIEITDGVIEPLPFAVPDFIADTPAAAQYATDIARVIAQDLVGSGLFREIPK
VAYISPYTDFDAEVNFTDWKAINAQALIAGAVSLSGDRITVRFRGYDVFAGQELGAGLQLSGTTDGWRRIAHKVADTIYS
RLTGEGGYFDSRVVFVSETGPKNDRKKRLAIMDYDGANVQYLTNSDAIVLAPRFSPTGDRVLYTSYESGFPQIHVLDVGR
VQRRVLSAGNGSMSFAPRFAPNGQTIVYSQSQGGNTDLFSMDINSGNPQRLTSAPSIETAPSFSPDGSQIVFESDRSGTS
QLYVMSASGGEAKRISFGQGRYGTPVWSPRGDYIAFTKQNAGRFHIGVMRTDGSEERLLTASFLDEGPTWSPNGRVIMFT
RETQGASGQARLYSVDISGRNLKPVKTPDGASDPSWSPLQN
>Mature_441_residues
MRNAIATVLLGLAMLLPVGAVQAQDGPLRIEITDGVIEPLPFAVPDFIADTPAAAQYATDIARVIAQDLVGSGLFREIPK
VAYISPYTDFDAEVNFTDWKAINAQALIAGAVSLSGDRITVRFRGYDVFAGQELGAGLQLSGTTDGWRRIAHKVADTIYS
RLTGEGGYFDSRVVFVSETGPKNDRKKRLAIMDYDGANVQYLTNSDAIVLAPRFSPTGDRVLYTSYESGFPQIHVLDVGR
VQRRVLSAGNGSMSFAPRFAPNGQTIVYSQSQGGNTDLFSMDINSGNPQRLTSAPSIETAPSFSPDGSQIVFESDRSGTS
QLYVMSASGGEAKRISFGQGRYGTPVWSPRGDYIAFTKQNAGRFHIGVMRTDGSEERLLTASFLDEGPTWSPNGRVIMFT
RETQGASGQARLYSVDISGRNLKPVKTPDGASDPSWSPLQN

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=418, Percent_Identity=29.4258373205742, Blast_Score=148, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_SILST (Q1GE19)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614359.1
- ProteinModelPortal:   Q1GE19
- SMR:   Q1GE19
- STRING:   Q1GE19
- GeneID:   4076484
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2365
- NMPDR:   fig|292414.1.peg.911
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q1GE19
- ProtClustDB:   PRK05137
- BioCyc:   SSP292414:TM1040_2365-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 47595; Mature: 47595

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNAIATVLLGLAMLLPVGAVQAQDGPLRIEITDGVIEPLPFAVPDFIADTPAAAQYATD
CCHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCCCHHHHCCCCCHHHHHHH
IARVIAQDLVGSGLFREIPKVAYISPYTDFDAEVNFTDWKAINAQALIAGAVSLSGDRIT
HHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCEECCCCCEECCCEEEEEEEEEECCCEEE
VRFRGYDVFAGQELGAGLQLSGTTDGWRRIAHKVADTIYSRLTGEGGYFDSRVVFVSETG
EEEECEEEECCHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
PKNDRKKRLAIMDYDGANVQYLTNSDAIVLAPRFSPTGDRVLYTSYESGFPQIHVLDVGR
CCCCCCCEEEEEECCCCCEEEEECCCEEEEEECCCCCCCEEEEEECCCCCCEEEEEECHH
VQRRVLSAGNGSMSFAPRFAPNGQTIVYSQSQGGNTDLFSMDINSGNPQRLTSAPSIETA
HHHHHHHCCCCCCEECCEECCCCCEEEEECCCCCCCEEEEEECCCCCCCEECCCCCCCCC
PSFSPDGSQIVFESDRSGTSQLYVMSASGGEAKRISFGQGRYGTPVWSPRGDYIAFTKQN
CCCCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECC
AGRFHIGVMRTDGSEERLLTASFLDEGPTWSPNGRVIMFTRETQGASGQARLYSVDISGR
CCEEEEEEEECCCCCCEEEEEEHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCC
NLKPVKTPDGASDPSWSPLQN
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNAIATVLLGLAMLLPVGAVQAQDGPLRIEITDGVIEPLPFAVPDFIADTPAAAQYATD
CCHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCCCHHHHCCCCCHHHHHHH
IARVIAQDLVGSGLFREIPKVAYISPYTDFDAEVNFTDWKAINAQALIAGAVSLSGDRIT
HHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCEECCCCCEECCCEEEEEEEEEECCCEEE
VRFRGYDVFAGQELGAGLQLSGTTDGWRRIAHKVADTIYSRLTGEGGYFDSRVVFVSETG
EEEECEEEECCHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
PKNDRKKRLAIMDYDGANVQYLTNSDAIVLAPRFSPTGDRVLYTSYESGFPQIHVLDVGR
CCCCCCCEEEEEECCCCCEEEEECCCEEEEEECCCCCCCEEEEEECCCCCCEEEEEECHH
VQRRVLSAGNGSMSFAPRFAPNGQTIVYSQSQGGNTDLFSMDINSGNPQRLTSAPSIETA
HHHHHHHCCCCCCEECCEECCCCCEEEEECCCCCCCEEEEEECCCCCCCEECCCCCCCCC
PSFSPDGSQIVFESDRSGTSQLYVMSASGGEAKRISFGQGRYGTPVWSPRGDYIAFTKQN
CCCCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECC
AGRFHIGVMRTDGSEERLLTASFLDEGPTWSPNGRVIMFTRETQGASGQARLYSVDISGR
CCEEEEEEEECCCCCCEEEEEEHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCC
NLKPVKTPDGASDPSWSPLQN
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA