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The map label for this gene is ppdK [H]

Identifier: 99082149

GI number: 99082149

Start: 2427681

End: 2430245

Strand: Reverse

Name: ppdK [H]

Synonym: TM1040_2309

Alternate gene names: 99082149

Gene position: 2430245-2427681 (Counterclockwise)

Preceding gene: 99082150

Following gene: 99082148

Centisome position: 75.92

GC content: 64.29

Gene sequence:

>2565_bases
ATGGACACCACCCCGAAGTCCACTTTGATCACCGCGACCGCACCGATCGCCAATCAGACCCATGGCGGGCGGGCCAAGTG
CCTGCAGCGGCTGGTTCGCTTGGAGCTACCGGTGCCGCAGACCGTGGCGCTCTCGTTTGATGCGGTGCATGAGATCGCCG
AGGGCTACATGGTGGATGTGAACGCGATCCTTGCGGATCTGCGACCCGGCGCGCTTTTGTGTGTGCGGCCCTCCTCCGAG
GATCCCGACTGGGGCGGTCCCGGAGCGGTTTTGAACATCGGCATGAATGACGCGCGCTACGTGGAGTTGAGCGACACGCT
GGGACCGGCGGCGGCGGCGGCGCTCTATTTGCGGTTTGTGCAGTCCTATGCGGTGCATGTGGCGCGGCTGGACCCGGATG
TGTTTGAGGACGTCGAGGACGGCGGCCCCGATGCGCTGAGCGAAATGCTGCAGGCCTATGAGGCTGAGGCTGAAGAGCCG
TTCCCCCAAGATCCGGGCGAGCAGCTGGCGGCGGTTCTGCGCTCCATGGCGCGCGCGTGGGAGGGCACGTCCGCGCGGCT
GTTGCGCGCCGCGAAAGGCGCGCCCGAAGACGCGGGGCTGGGTCTCATTGTGCAAGAGATGGTGCCGGGGCTGGGGCAGG
GTGAATGCGGTTCAGGCGTGTTGCAGCTTGTGAATTCGCAGACCGGGCTGCCGCAGATCACCGGACGCTATCTGAGCCAA
AGCCAGGGGCGCGACGCGCTCGGGGTTGATGCGGAGGCAATCTATCTCGAGCAGGACGAACGCGGCCCCTCGCTTGAGGA
GCTGGCGCCCGAGGCCTTTGCCGAGTTGAAGGCCCATGCGGCGCTGATGCGTAGAAAACTGCGTGCCGAAATGCAGGTGG
AATTCGTCATCGAAAATGGCCGTGTGCATATTCTCGACGGGGTGCGGGTACCACGTTCTTCGCGCGCGGCGGTGCGGATT
GCGGTGCGGCTGGCAGAGGACGGGATCATTCCCGCCGAAGAGGCGCTGATGCGGGTGGATGCCTATTCGCTCAACGAATT
GCTGCACCGCCAGGTGGACCCGAATGCCAAACGGGACGTGATTGGCAGCGGCATTGCCGCAAGTCCGGGCGCGGCCACCG
GGCGTCTGGTTTTCACCGCGACCGAGGCGCAGGCCAGCAAGTCGCGTGGCGAACCCTGCATTCTGGCGCGACGTGAGACT
TCGCCAGAGGATGTGCGGGGCATGCATGCGGCTGCGGGTGTACTGACGGAAAAAGGCGGAATGACCAGCCATGCGGCGGT
GATTGGTCGCGGGCTGGGGCTGCCCTGTATCGTGGGCGCGTCGCGCCTGCGGTTCGACAGTCGGCGCAAACAGCTGACGG
CCCCGGATGGGCGAGTTTTCAAGGCAGGCGAGATCATCACCGTGGATGGCACCTCGGGCGAGGTGCTTGCTGGAGAACCC
GCCATGGTCGAGGCCGCCGAGGATGATGCGCTGGTGACGTTGATGGCCTGGGCGGATGCGGCGCGCGACATTGCGATCCG
CGCCAATGCCGACACGCCTGCCGATGCGCAGACCGCGCGCAATTTCAACGCCGAGGGTATTGGTCTGTGCCGGACCGAGC
ACATGTTCTTTGATCCGGGTCGCCTTACGGTGATGCGCGAGATGATCTTTGCCGAGACCTCTTCCGGGCGGGCGGCGGTT
CTGGCGCGTCTCCTGCCGATGCAGCGCAAGGATTTTCGCGAGCTTTTCCGTATCATGGAAGGTCTGCCGGTGTGCATCCG
GCTGTTTGACCCGCCATTGCATGAATTCCTGCCCACCACCACTGCCGGACAGCGCGAACTCTCCGAGGCTCTGGGCCTGC
CGGTGAGCGACGTGACACGCCGGGTTGCGGAAATGGGGGAGTACAACCCCATGCTGGGTCTGCGCGGCGTGCGTCTCGGG
GTGACGGTGCCCGAGATCTACGACATGCAGGCGCGCGCGATCTTTGAAGCCACGCTCGAGGCCTCGCGCGATGGGGCGCC
AGTGGTGCCTGAGATCATGATCCCGTTGGTGTCTGCCAAACGCGAAGTGGAACTGGTCAAGGCGCGTATCGATGCGGTGG
CCGCAGCCGTGAAATCCGAGCGCGGCGCCGATTTCACCTATCGGCTCGGTGTGATGGTCGAGACCCCACGGGCGGCCCTC
CGGGCCGATGAGATTGCCGCGCATACGGCCTTCATGAGCTTTGGCACCAACGATCTCACGCAGATGACCTATGGTCTGTC
GCGCGATGATGCGGGACGGTTCATGTCGGCCTATGTGCAGCAGGGCGTCTTTCCCGAAGATCCCTTCCATGTGCTGGATG
TGGACGGCGTCGGAGAGCTGTTGCAACTCGGGGTGCAGCGGGGGCGGATGGCGAAGCCCGAAATCACCCTGTCGATCTGT
GGTGAACACGGTGGCAATCCCGAATCAATCGCGTTTTGCCGCGAGGCGGGCTTTGATTACGTGTCTTGCTCGCCCTTCCG
GGTGCCTGTCGCGCGGCTTGCGGCAGCACAATTGGCGATCGCGGATAAAACCGGCCAAATTGCGCCGACATACATAAAAA
ATTAA

Upstream 100 bases:

>100_bases
AGCAGGGGCGACGTGCTTTTCCTTGCGGGACGGCCTGCGCGCATTATGCTCAGGGGCAGGGAGGATCGCTGCGTTGCAGA
AACAGACAGGGGACGAACCG

Downstream 100 bases:

>100_bases
CGATTTCAGGGTTCTTCGTCGGCAAAAGACACATCGCCGCATCAAGATGTACCTTGGGGCATAACGGGTATACGTTCGCT
TGGGTGGACGTTTTGATACA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 854; Mature: 854

Protein sequence:

>854_residues
MDTTPKSTLITATAPIANQTHGGRAKCLQRLVRLELPVPQTVALSFDAVHEIAEGYMVDVNAILADLRPGALLCVRPSSE
DPDWGGPGAVLNIGMNDARYVELSDTLGPAAAAALYLRFVQSYAVHVARLDPDVFEDVEDGGPDALSEMLQAYEAEAEEP
FPQDPGEQLAAVLRSMARAWEGTSARLLRAAKGAPEDAGLGLIVQEMVPGLGQGECGSGVLQLVNSQTGLPQITGRYLSQ
SQGRDALGVDAEAIYLEQDERGPSLEELAPEAFAELKAHAALMRRKLRAEMQVEFVIENGRVHILDGVRVPRSSRAAVRI
AVRLAEDGIIPAEEALMRVDAYSLNELLHRQVDPNAKRDVIGSGIAASPGAATGRLVFTATEAQASKSRGEPCILARRET
SPEDVRGMHAAAGVLTEKGGMTSHAAVIGRGLGLPCIVGASRLRFDSRRKQLTAPDGRVFKAGEIITVDGTSGEVLAGEP
AMVEAAEDDALVTLMAWADAARDIAIRANADTPADAQTARNFNAEGIGLCRTEHMFFDPGRLTVMREMIFAETSSGRAAV
LARLLPMQRKDFRELFRIMEGLPVCIRLFDPPLHEFLPTTTAGQRELSEALGLPVSDVTRRVAEMGEYNPMLGLRGVRLG
VTVPEIYDMQARAIFEATLEASRDGAPVVPEIMIPLVSAKREVELVKARIDAVAAAVKSERGADFTYRLGVMVETPRAAL
RADEIAAHTAFMSFGTNDLTQMTYGLSRDDAGRFMSAYVQQGVFPEDPFHVLDVDGVGELLQLGVQRGRMAKPEITLSIC
GEHGGNPESIAFCREAGFDYVSCSPFRVPVARLAAAQLAIADKTGQIAPTYIKN

Sequences:

>Translated_854_residues
MDTTPKSTLITATAPIANQTHGGRAKCLQRLVRLELPVPQTVALSFDAVHEIAEGYMVDVNAILADLRPGALLCVRPSSE
DPDWGGPGAVLNIGMNDARYVELSDTLGPAAAAALYLRFVQSYAVHVARLDPDVFEDVEDGGPDALSEMLQAYEAEAEEP
FPQDPGEQLAAVLRSMARAWEGTSARLLRAAKGAPEDAGLGLIVQEMVPGLGQGECGSGVLQLVNSQTGLPQITGRYLSQ
SQGRDALGVDAEAIYLEQDERGPSLEELAPEAFAELKAHAALMRRKLRAEMQVEFVIENGRVHILDGVRVPRSSRAAVRI
AVRLAEDGIIPAEEALMRVDAYSLNELLHRQVDPNAKRDVIGSGIAASPGAATGRLVFTATEAQASKSRGEPCILARRET
SPEDVRGMHAAAGVLTEKGGMTSHAAVIGRGLGLPCIVGASRLRFDSRRKQLTAPDGRVFKAGEIITVDGTSGEVLAGEP
AMVEAAEDDALVTLMAWADAARDIAIRANADTPADAQTARNFNAEGIGLCRTEHMFFDPGRLTVMREMIFAETSSGRAAV
LARLLPMQRKDFRELFRIMEGLPVCIRLFDPPLHEFLPTTTAGQRELSEALGLPVSDVTRRVAEMGEYNPMLGLRGVRLG
VTVPEIYDMQARAIFEATLEASRDGAPVVPEIMIPLVSAKREVELVKARIDAVAAAVKSERGADFTYRLGVMVETPRAAL
RADEIAAHTAFMSFGTNDLTQMTYGLSRDDAGRFMSAYVQQGVFPEDPFHVLDVDGVGELLQLGVQRGRMAKPEITLSIC
GEHGGNPESIAFCREAGFDYVSCSPFRVPVARLAAAQLAIADKTGQIAPTYIKN
>Mature_854_residues
MDTTPKSTLITATAPIANQTHGGRAKCLQRLVRLELPVPQTVALSFDAVHEIAEGYMVDVNAILADLRPGALLCVRPSSE
DPDWGGPGAVLNIGMNDARYVELSDTLGPAAAAALYLRFVQSYAVHVARLDPDVFEDVEDGGPDALSEMLQAYEAEAEEP
FPQDPGEQLAAVLRSMARAWEGTSARLLRAAKGAPEDAGLGLIVQEMVPGLGQGECGSGVLQLVNSQTGLPQITGRYLSQ
SQGRDALGVDAEAIYLEQDERGPSLEELAPEAFAELKAHAALMRRKLRAEMQVEFVIENGRVHILDGVRVPRSSRAAVRI
AVRLAEDGIIPAEEALMRVDAYSLNELLHRQVDPNAKRDVIGSGIAASPGAATGRLVFTATEAQASKSRGEPCILARRET
SPEDVRGMHAAAGVLTEKGGMTSHAAVIGRGLGLPCIVGASRLRFDSRRKQLTAPDGRVFKAGEIITVDGTSGEVLAGEP
AMVEAAEDDALVTLMAWADAARDIAIRANADTPADAQTARNFNAEGIGLCRTEHMFFDPGRLTVMREMIFAETSSGRAAV
LARLLPMQRKDFRELFRIMEGLPVCIRLFDPPLHEFLPTTTAGQRELSEALGLPVSDVTRRVAEMGEYNPMLGLRGVRLG
VTVPEIYDMQARAIFEATLEASRDGAPVVPEIMIPLVSAKREVELVKARIDAVAAAVKSERGADFTYRLGVMVETPRAAL
RADEIAAHTAFMSFGTNDLTQMTYGLSRDDAGRFMSAYVQQGVFPEDPFHVLDVDGVGELLQLGVQRGRMAKPEITLSIC
GEHGGNPESIAFCREAGFDYVSCSPFRVPVARLAAAQLAIADKTGQIAPTYIKN

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=432, Percent_Identity=27.0833333333333, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789193, Length=428, Percent_Identity=25, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI48994992, Length=455, Percent_Identity=25.0549450549451, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788726, Length=466, Percent_Identity=24.4635193133047, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 91717; Mature: 91717

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTPKSTLITATAPIANQTHGGRAKCLQRLVRLELPVPQTVALSFDAVHEIAEGYMVDV
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCEEEEH
NAILADLRPGALLCVRPSSEDPDWGGPGAVLNIGMNDARYVELSDTLGPAAAAALYLRFV
HHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHH
QSYAVHVARLDPDVFEDVEDGGPDALSEMLQAYEAEAEEPFPQDPGEQLAAVLRSMARAW
HHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
EGTSARLLRAAKGAPEDAGLGLIVQEMVPGLGQGECGSGVLQLVNSQTGLPQITGRYLSQ
CCCHHHHHHHHCCCCCCCCCCEEHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
SQGRDALGVDAEAIYLEQDERGPSLEELAPEAFAELKAHAALMRRKLRAEMQVEFVIENG
CCCCCCCCCCCEEEEEECCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHCEEEEEEEECC
RVHILDGVRVPRSSRAAVRIAVRLAEDGIIPAEEALMRVDAYSLNELLHRQVDPNAKRDV
CEEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IGSGIAASPGAATGRLVFTATEAQASKSRGEPCILARRETSPEDVRGMHAAAGVLTEKGG
HCCCCCCCCCCCCCEEEEEECHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
MTSHAAVIGRGLGLPCIVGASRLRFDSRRKQLTAPDGRVFKAGEIITVDGTSGEVLAGEP
CCHHHHHHHCCCCCHHHHCHHHHHHHHHHHCCCCCCCCEEECCCEEEEECCCCCEEECCC
AMVEAAEDDALVTLMAWADAARDIAIRANADTPADAQTARNFNAEGIGLCRTEHMFFDPG
CEEEECCCCCEEEEEEHHCCCCCEEEEECCCCCCCHHHHCCCCCCCCEEEEECCEEECCC
RLTVMREMIFAETSSGRAAVLARLLPMQRKDFRELFRIMEGLPVCIRLFDPPLHEFLPTT
HHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCC
TAGQRELSEALGLPVSDVTRRVAEMGEYNPMLGLRGVRLGVTVPEIYDMQARAIFEATLE
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
ASRDGAPVVPEIMIPLVSAKREVELVKARIDAVAAAVKSERGADFTYRLGVMVETPRAAL
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHH
RADEIAAHTAFMSFGTNDLTQMTYGLSRDDAGRFMSAYVQQGVFPEDPFHVLDVDGVGEL
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCHHHH
LQLGVQRGRMAKPEITLSICGEHGGNPESIAFCREAGFDYVSCSPFRVPVARLAAAQLAI
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHE
ADKTGQIAPTYIKN
ECCCCCCCCHHCCC
>Mature Secondary Structure
MDTTPKSTLITATAPIANQTHGGRAKCLQRLVRLELPVPQTVALSFDAVHEIAEGYMVDV
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCEEEEH
NAILADLRPGALLCVRPSSEDPDWGGPGAVLNIGMNDARYVELSDTLGPAAAAALYLRFV
HHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHH
QSYAVHVARLDPDVFEDVEDGGPDALSEMLQAYEAEAEEPFPQDPGEQLAAVLRSMARAW
HHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
EGTSARLLRAAKGAPEDAGLGLIVQEMVPGLGQGECGSGVLQLVNSQTGLPQITGRYLSQ
CCCHHHHHHHHCCCCCCCCCCEEHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
SQGRDALGVDAEAIYLEQDERGPSLEELAPEAFAELKAHAALMRRKLRAEMQVEFVIENG
CCCCCCCCCCCEEEEEECCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHCEEEEEEEECC
RVHILDGVRVPRSSRAAVRIAVRLAEDGIIPAEEALMRVDAYSLNELLHRQVDPNAKRDV
CEEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IGSGIAASPGAATGRLVFTATEAQASKSRGEPCILARRETSPEDVRGMHAAAGVLTEKGG
HCCCCCCCCCCCCCEEEEEECHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
MTSHAAVIGRGLGLPCIVGASRLRFDSRRKQLTAPDGRVFKAGEIITVDGTSGEVLAGEP
CCHHHHHHHCCCCCHHHHCHHHHHHHHHHHCCCCCCCCEEECCCEEEEECCCCCEEECCC
AMVEAAEDDALVTLMAWADAARDIAIRANADTPADAQTARNFNAEGIGLCRTEHMFFDPG
CEEEECCCCCEEEEEEHHCCCCCEEEEECCCCCCCHHHHCCCCCCCCEEEEECCEEECCC
RLTVMREMIFAETSSGRAAVLARLLPMQRKDFRELFRIMEGLPVCIRLFDPPLHEFLPTT
HHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCC
TAGQRELSEALGLPVSDVTRRVAEMGEYNPMLGLRGVRLGVTVPEIYDMQARAIFEATLE
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
ASRDGAPVVPEIMIPLVSAKREVELVKARIDAVAAAVKSERGADFTYRLGVMVETPRAAL
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHH
RADEIAAHTAFMSFGTNDLTQMTYGLSRDDAGRFMSAYVQQGVFPEDPFHVLDVDGVGEL
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCHHHH
LQLGVQRGRMAKPEITLSICGEHGGNPESIAFCREAGFDYVSCSPFRVPVARLAAAQLAI
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHE
ADKTGQIAPTYIKN
ECCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]