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The map label for this gene is glyS

Identifier: 99082150

GI number: 99082150

Start: 2430406

End: 2432469

Strand: Reverse

Name: glyS

Synonym: TM1040_2310

Alternate gene names: 99082150

Gene position: 2432469-2430406 (Counterclockwise)

Preceding gene: 99082151

Following gene: 99082149

Centisome position: 75.99

GC content: 62.02

Gene sequence:

>2064_bases
ATGGCTGATCTTCTGATCGAACTTTTCTCCGAGGAAATCCCCGCCCGCATGCAGGCGCGCGCGGGCGAAGATTTGAAGAA
GCGGATGACCGACGGTCTGGTCGAGGCGGGGCTGACCTATGCCGGGGCTCATGCGCTGACGACGCCGCGCCGCCTGACGC
TGGCCATCGAGGGTCTCCTGGATCATTCCCCCACCGTGCGCGAAGAGCGCAAAGGCCCCAAGGTCGGCGCGCCCGAGAAG
GCCATCGAGGGCTTTTTGCGCGGTGCGGGGCTGACACGCGACCAGCTTGAGGAACGCGACACCCCCAAAGGTGCGGTCTA
CTTTGCCACCATCGAAAAGCCGGGCCGCCCGGCGGCCGAGATCATTGCAGAGGTGCTCGAAGACACCATTCGCAATTTCC
CTTGGCCCAAGTCGATGCGCTGGGGGGCAGGCGCTTTGCGGTGGGTGCGGCCGTTGCACTCCATCCTGTGCCTTCTGACC
CGCGAAGAGGGCAGCGAGGTGGTCCCGCTGGAGATCGACGGTGTCACATCGGGCGACAGCACCGCGGGTCATCGGTTCAT
GGCGCCCGCGCGCTTTGCCGTGACGGGATTTGAGGACTACGCCGCCAAGCTCAAACGCGCCTTCGTGGTGCTCGACCCCA
AGGAGCGCGAGGCGGCGATCTGGCAGGATGCCACAAATCAGGCCTTTGCCTCGGGTCTTGAGGTTGTCGATGACAAGGGT
CTTCTGGCCGAGGTTGCAGGGCTGGTGGAATGGCCGGTGGTTCTGATGGGCTCGATTGATGCGGAGTTCCTCGACCTGCC
GCCGGAGGTGCTGCAGACCTCTATGAAAGAGCACCAGAAGTTCTTCTCGGTAAAGAATCCCAAGACCGGGCGGATTGAGA
AATTCATCACCGTGGCCAACCGCGAAACCGCAGACAATGGCGCGACCATTCTGGCGGGCAACCAAAAGGTGCTGAGCGCG
CGTCTGGCAGATGCGAAGTTCTTCTGGGAGAACGACCTGCGCATCGCAAAATCCGAGGTCGGCGTGGAAGGCTGGCTCGA
GAGCCTTGGCAATGTGACCTTCCACAACAAGCTTGGCACTCAGGCCGCGCGCATCGACCGGATTGCGGCGCTGGCACGCG
AGATTGCCCCCGTTGTGGGTGCGGATGCGGATCTGGCGGAACAGGCGGCGAAGGTCGCCAAGGCCGACCTCAGCTCCGAG
ATGGTTTATGAGTTTCCTGAACTGCAGGGCCTGATGGGCCGCTATTACGCTCAGGCTGCCGGGCTGCCGCAAGAGGTCGC
CAATGCCTGTGAGCTGCATTATTCGCCCCTGGGCCCCAGCGATGATGTGCCGACAGAGCCCGTTTCGGTCGCCGTGGCGC
TTGCGGATAAGATCGACACGCTCACCGGGTTCTGGGCGATTGATGAAAAGCCCACCGGGTCCAAAGACCCCTACGCGCTG
CGCCGTGCGGCACTCGGGGTTATTCGGTTGGTGCTCTCGAACGATGTGCGAGTGGGTTTGAAAGAAGTCTTTGCAAAAGC
CTATGAGGGCGCGGATGGGTCGGATCTTCTGTCGTTCTTCCACGACCGCCTCAAGGTCTTTCTGCGCGATCAGGGGATTC
GCCATGATGTGATCGACGCCTGCATCGCGATGGAGGGCAGTGACGATCTGACACTTTTGGTGAAACGCGCCCGCGCGCTT
GAGGATTTTATGAAATCCGAGGATGGCGAGAACCTGCTGCAAGGGTTCAAACGCGCCAACAACATATTGTCGCAGGCCGA
GGAAAAAGACGGCGTCGAATATTCCTTTGGCGCGGACAAGAAATTCGTCGAGGATCCTGCCGAGGCGGCGCTCTTTGAAG
CGCTGGCTGCAAACGAGGCAGCGATCTCCACGGCCATCGAGGCCGAGGATTTCGCGGCCGCCATGGGCGGCATGGCCGCA
TTGCGTGCGCCGGTGGATGCCTTCTTTGAGGCGGTGCAGGTCAATTCCGACAATCCGGTAGTGCGCCGCAACCGGCTCAA
TCTCCTCAGCCAGATCCGCAAGGTCTGCGGTCAGGTGGCGGACCTGAGCCGCATCGAGGGCTGA

Upstream 100 bases:

>100_bases
CCAAGCGGCCGCAAAGCAACTGATCCGGCGCAGCTCTGCCTGTCGCCACATCCCTTTATCGCCGTGAGCGCACGGCAGAC
CGAACCGTTGAGGACACGTC

Downstream 100 bases:

>100_bases
TCGCGCAGACATATTTTGCACGCTTCGACCCTCGCCAGATATGGCGGGGGTCGTTTTTGTAGCAGGGGCGACGTGCTTTT
CCTTGCGGGACGGCCTGCGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MADLLIELFSEEIPARMQARAGEDLKKRMTDGLVEAGLTYAGAHALTTPRRLTLAIEGLLDHSPTVREERKGPKVGAPEK
AIEGFLRGAGLTRDQLEERDTPKGAVYFATIEKPGRPAAEIIAEVLEDTIRNFPWPKSMRWGAGALRWVRPLHSILCLLT
REEGSEVVPLEIDGVTSGDSTAGHRFMAPARFAVTGFEDYAAKLKRAFVVLDPKEREAAIWQDATNQAFASGLEVVDDKG
LLAEVAGLVEWPVVLMGSIDAEFLDLPPEVLQTSMKEHQKFFSVKNPKTGRIEKFITVANRETADNGATILAGNQKVLSA
RLADAKFFWENDLRIAKSEVGVEGWLESLGNVTFHNKLGTQAARIDRIAALAREIAPVVGADADLAEQAAKVAKADLSSE
MVYEFPELQGLMGRYYAQAAGLPQEVANACELHYSPLGPSDDVPTEPVSVAVALADKIDTLTGFWAIDEKPTGSKDPYAL
RRAALGVIRLVLSNDVRVGLKEVFAKAYEGADGSDLLSFFHDRLKVFLRDQGIRHDVIDACIAMEGSDDLTLLVKRARAL
EDFMKSEDGENLLQGFKRANNILSQAEEKDGVEYSFGADKKFVEDPAEAALFEALAANEAAISTAIEAEDFAAAMGGMAA
LRAPVDAFFEAVQVNSDNPVVRRNRLNLLSQIRKVCGQVADLSRIEG

Sequences:

>Translated_687_residues
MADLLIELFSEEIPARMQARAGEDLKKRMTDGLVEAGLTYAGAHALTTPRRLTLAIEGLLDHSPTVREERKGPKVGAPEK
AIEGFLRGAGLTRDQLEERDTPKGAVYFATIEKPGRPAAEIIAEVLEDTIRNFPWPKSMRWGAGALRWVRPLHSILCLLT
REEGSEVVPLEIDGVTSGDSTAGHRFMAPARFAVTGFEDYAAKLKRAFVVLDPKEREAAIWQDATNQAFASGLEVVDDKG
LLAEVAGLVEWPVVLMGSIDAEFLDLPPEVLQTSMKEHQKFFSVKNPKTGRIEKFITVANRETADNGATILAGNQKVLSA
RLADAKFFWENDLRIAKSEVGVEGWLESLGNVTFHNKLGTQAARIDRIAALAREIAPVVGADADLAEQAAKVAKADLSSE
MVYEFPELQGLMGRYYAQAAGLPQEVANACELHYSPLGPSDDVPTEPVSVAVALADKIDTLTGFWAIDEKPTGSKDPYAL
RRAALGVIRLVLSNDVRVGLKEVFAKAYEGADGSDLLSFFHDRLKVFLRDQGIRHDVIDACIAMEGSDDLTLLVKRARAL
EDFMKSEDGENLLQGFKRANNILSQAEEKDGVEYSFGADKKFVEDPAEAALFEALAANEAAISTAIEAEDFAAAMGGMAA
LRAPVDAFFEAVQVNSDNPVVRRNRLNLLSQIRKVCGQVADLSRIEG
>Mature_686_residues
ADLLIELFSEEIPARMQARAGEDLKKRMTDGLVEAGLTYAGAHALTTPRRLTLAIEGLLDHSPTVREERKGPKVGAPEKA
IEGFLRGAGLTRDQLEERDTPKGAVYFATIEKPGRPAAEIIAEVLEDTIRNFPWPKSMRWGAGALRWVRPLHSILCLLTR
EEGSEVVPLEIDGVTSGDSTAGHRFMAPARFAVTGFEDYAAKLKRAFVVLDPKEREAAIWQDATNQAFASGLEVVDDKGL
LAEVAGLVEWPVVLMGSIDAEFLDLPPEVLQTSMKEHQKFFSVKNPKTGRIEKFITVANRETADNGATILAGNQKVLSAR
LADAKFFWENDLRIAKSEVGVEGWLESLGNVTFHNKLGTQAARIDRIAALAREIAPVVGADADLAEQAAKVAKADLSSEM
VYEFPELQGLMGRYYAQAAGLPQEVANACELHYSPLGPSDDVPTEPVSVAVALADKIDTLTGFWAIDEKPTGSKDPYALR
RAALGVIRLVLSNDVRVGLKEVFAKAYEGADGSDLLSFFHDRLKVFLRDQGIRHDVIDACIAMEGSDDLTLLVKRARALE
DFMKSEDGENLLQGFKRANNILSQAEEKDGVEYSFGADKKFVEDPAEAALFEALAANEAAISTAIEAEDFAAAMGGMAAL
RAPVDAFFEAVQVNSDNPVVRRNRLNLLSQIRKVCGQVADLSRIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=699, Percent_Identity=34.1917024320458, Blast_Score=364, Evalue=1e-101,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_SILST (Q1GE74)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614304.1
- ProteinModelPortal:   Q1GE74
- STRING:   Q1GE74
- GeneID:   4078300
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2310
- NMPDR:   fig|292414.1.peg.672
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- PhylomeDB:   Q1GE74
- ProtClustDB:   PRK01233
- BioCyc:   SSP292414:TM1040_2310-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74816; Mature: 74685

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLLIELFSEEIPARMQARAGEDLKKRMTDGLVEAGLTYAGAHALTTPRRLTLAIEGLL
CHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCHHHCCHHHCCCHHHHHHHHHHH
DHSPTVREERKGPKVGAPEKAIEGFLRGAGLTRDQLEERDTPKGAVYFATIEKPGRPAAE
CCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEEECCCCCCHHH
IIAEVLEDTIRNFPWPKSMRWGAGALRWVRPLHSILCLLTREEGSEVVPLEIDGVTSGDS
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
TAGHRFMAPARFAVTGFEDYAAKLKRAFVVLDPKEREAAIWQDATNQAFASGLEVVDDKG
CCCCCCCCCCHHEECCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCHHHCCCC
LLAEVAGLVEWPVVLMGSIDAEFLDLPPEVLQTSMKEHQKFFSVKNPKTGRIEKFITVAN
HHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEECC
RETADNGATILAGNQKVLSARLADAKFFWENDLRIAKSEVGVEGWLESLGNVTFHNKLGT
CCCCCCCCEEEECCHHHHHHHHHHHHEEECCCCHHHHHHCCHHHHHHHHCCEEEEHHHCH
QAARIDRIAALAREIAPVVGADADLAEQAAKVAKADLSSEMVYEFPELQGLMGRYYAQAA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GLPQEVANACELHYSPLGPSDDVPTEPVSVAVALADKIDTLTGFWAIDEKPTGSKDPYAL
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHH
RRAALGVIRLVLSNDVRVGLKEVFAKAYEGADGSDLLSFFHDRLKVFLRDQGIRHDVIDA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
CIAMEGSDDLTLLVKRARALEDFMKSEDGENLLQGFKRANNILSQAEEKDGVEYSFGADK
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
KFVEDPAEAALFEALAANEAAISTAIEAEDFAAAMGGMAALRAPVDAFFEAVQVNSDNPV
HHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VRRNRLNLLSQIRKVCGQVADLSRIEG
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADLLIELFSEEIPARMQARAGEDLKKRMTDGLVEAGLTYAGAHALTTPRRLTLAIEGLL
HHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCHHHCCHHHCCCHHHHHHHHHHH
DHSPTVREERKGPKVGAPEKAIEGFLRGAGLTRDQLEERDTPKGAVYFATIEKPGRPAAE
CCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEEECCCCCCHHH
IIAEVLEDTIRNFPWPKSMRWGAGALRWVRPLHSILCLLTREEGSEVVPLEIDGVTSGDS
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
TAGHRFMAPARFAVTGFEDYAAKLKRAFVVLDPKEREAAIWQDATNQAFASGLEVVDDKG
CCCCCCCCCCHHEECCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCHHHCCCC
LLAEVAGLVEWPVVLMGSIDAEFLDLPPEVLQTSMKEHQKFFSVKNPKTGRIEKFITVAN
HHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEECC
RETADNGATILAGNQKVLSARLADAKFFWENDLRIAKSEVGVEGWLESLGNVTFHNKLGT
CCCCCCCCEEEECCHHHHHHHHHHHHEEECCCCHHHHHHCCHHHHHHHHCCEEEEHHHCH
QAARIDRIAALAREIAPVVGADADLAEQAAKVAKADLSSEMVYEFPELQGLMGRYYAQAA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GLPQEVANACELHYSPLGPSDDVPTEPVSVAVALADKIDTLTGFWAIDEKPTGSKDPYAL
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHH
RRAALGVIRLVLSNDVRVGLKEVFAKAYEGADGSDLLSFFHDRLKVFLRDQGIRHDVIDA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
CIAMEGSDDLTLLVKRARALEDFMKSEDGENLLQGFKRANNILSQAEEKDGVEYSFGADK
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
KFVEDPAEAALFEALAANEAAISTAIEAEDFAAAMGGMAALRAPVDAFFEAVQVNSDNPV
HHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VRRNRLNLLSQIRKVCGQVADLSRIEG
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA