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The map label for this gene is carA [H]

Identifier: 99082134

GI number: 99082134

Start: 2412716

End: 2413876

Strand: Direct

Name: carA [H]

Synonym: TM1040_2294

Alternate gene names: 99082134

Gene position: 2412716-2413876 (Clockwise)

Preceding gene: 99082132

Following gene: 99082135

Centisome position: 75.38

GC content: 64.86

Gene sequence:

>1161_bases
ATGGTCGAAACCGTTTCGCCGAAGCCAACCGCCTGCCTGGCCCTTGCAGATGGCACCGTGTTCTATGGACACGGGTTTGG
CGCCACCGGGCGATGCGTGGCCGAGCTGTGCTTTAACACCGCAATGACCGGCTATCAGGAAATCATGACCGACCCTTCCT
ATGCAGGTCAGGTCGTCACCTTCACCTTCCCCCACATCGGCAACACCGGCGTCACCCCCGAGGATGACGAAACCGCCGAC
CCGGTGGCCGCAGGCATGGTGGTGAAATGGGATCCGACGCTGTCCTCCAACTGGCGCGCCACCGAAGAGCTGAAGTCCTG
GCTCACCCGCACGGGCCGCATCGCCATCGGCGGCGTGGACACCCGCCGTCTGACTCGCGCGATCCGCCAGCAGGGCGCGC
CGCATGTGGCGATGGAGCATAACCCGGACGGGAATTTCGATCTTGAGGCGCTGGTCGCCGCCGCCCGCGCCTGGCCCGGC
CTTGAGGGCATGGACCTCGCCAAGGACGTGACCTGCGCGCAGTCCTACCGCTGGGATGAGATGCGTTGGGCCTGGCCCGA
GGGCTACACCCGTCAGGAAGAGCCCAAGCACAAGGTGGTCGCCATCGACTATGGTGCCAAGCGCAACATCCTGCGCTGCC
TCGCCTCGGCGGGCTGCGATGTCACCGTGCTGCCGGCCACCGCAACCTCGGAAGAGGTACTGGCCCATGGCCCTGATGGT
GTGTTCCTCTCCAATGGCCCCGGCGACCCGGCCGCAACCGGCGCATACGCTGTGCCGATGATCAAGGAAATCCTGGATAA
GACCGACTTGCCGGTCTTTGGGATCTGTCTGGGCCACCAGATGCTCGCACTCGCTTTGGGGGCCAAGACCACCAAGATGA
ACCACGGCCACCACGGCGCCAACCACCCGGTCAAGGAACACGGCACCGGCAAGGTGGAGATCACGTCGATGAACCACGGC
TTTGCAGTGGATGCTCAAACCCTGCCCGAGGGCGTCGAAGAGACCCATGTCTCGCTGTTTGACGGCTCCAACTGCGGCAT
TCGCATGACCGATCGCCCGGTCTACTCCGTGCAGCACCACCCCGAGGCCAGCCCCGGCCCGCAGGACAGTTTCTATCTGT
TCGAGCGCTTTGCAGAGGCGATGGCCGCGCGCAAGGCCTGA

Upstream 100 bases:

>100_bases
TTGTGCGAGACGCAGGGCCGGTATCGGCCTATCGGTGCTTGACCCCGCGCGGCGCGGGACTTATTGATGCGCGAATTTTG
CCCGCATGGAGAGTCGAGTC

Downstream 100 bases:

>100_bases
TCGAGCCGCCGTTTCAGTTCTGACACCGCCCGGTTGCCATGCAATCGGGCGGTGTCTTTTTGCGGCGGCTTTTCATCTAT
TGCGGGTGTTTAACTACGGT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MVETVSPKPTACLALADGTVFYGHGFGATGRCVAELCFNTAMTGYQEIMTDPSYAGQVVTFTFPHIGNTGVTPEDDETAD
PVAAGMVVKWDPTLSSNWRATEELKSWLTRTGRIAIGGVDTRRLTRAIRQQGAPHVAMEHNPDGNFDLEALVAAARAWPG
LEGMDLAKDVTCAQSYRWDEMRWAWPEGYTRQEEPKHKVVAIDYGAKRNILRCLASAGCDVTVLPATATSEEVLAHGPDG
VFLSNGPGDPAATGAYAVPMIKEILDKTDLPVFGICLGHQMLALALGAKTTKMNHGHHGANHPVKEHGTGKVEITSMNHG
FAVDAQTLPEGVEETHVSLFDGSNCGIRMTDRPVYSVQHHPEASPGPQDSFYLFERFAEAMAARKA

Sequences:

>Translated_386_residues
MVETVSPKPTACLALADGTVFYGHGFGATGRCVAELCFNTAMTGYQEIMTDPSYAGQVVTFTFPHIGNTGVTPEDDETAD
PVAAGMVVKWDPTLSSNWRATEELKSWLTRTGRIAIGGVDTRRLTRAIRQQGAPHVAMEHNPDGNFDLEALVAAARAWPG
LEGMDLAKDVTCAQSYRWDEMRWAWPEGYTRQEEPKHKVVAIDYGAKRNILRCLASAGCDVTVLPATATSEEVLAHGPDG
VFLSNGPGDPAATGAYAVPMIKEILDKTDLPVFGICLGHQMLALALGAKTTKMNHGHHGANHPVKEHGTGKVEITSMNHG
FAVDAQTLPEGVEETHVSLFDGSNCGIRMTDRPVYSVQHHPEASPGPQDSFYLFERFAEAMAARKA
>Mature_386_residues
MVETVSPKPTACLALADGTVFYGHGFGATGRCVAELCFNTAMTGYQEIMTDPSYAGQVVTFTFPHIGNTGVTPEDDETAD
PVAAGMVVKWDPTLSSNWRATEELKSWLTRTGRIAIGGVDTRRLTRAIRQQGAPHVAMEHNPDGNFDLEALVAAARAWPG
LEGMDLAKDVTCAQSYRWDEMRWAWPEGYTRQEEPKHKVVAIDYGAKRNILRCLASAGCDVTVLPATATSEEVLAHGPDG
VFLSNGPGDPAATGAYAVPMIKEILDKTDLPVFGICLGHQMLALALGAKTTKMNHGHHGANHPVKEHGTGKVEITSMNHG
FAVDAQTLPEGVEETHVSLFDGSNCGIRMTDRPVYSVQHHPEASPGPQDSFYLFERFAEAMAARKA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=37.597911227154, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI169790915, Length=394, Percent_Identity=33.248730964467, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI21361331, Length=394, Percent_Identity=33.248730964467, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=53.4391534391534, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI193204318, Length=394, Percent_Identity=34.010152284264, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324878, Length=390, Percent_Identity=37.6923076923077, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=399, Percent_Identity=34.8370927318296, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI45555749, Length=396, Percent_Identity=36.1111111111111, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24642586, Length=396, Percent_Identity=36.1111111111111, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41645; Mature: 41645

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVETVSPKPTACLALADGTVFYGHGFGATGRCVAELCFNTAMTGYQEIMTDPSYAGQVVT
CCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
FTFPHIGNTGVTPEDDETADPVAAGMVVKWDPTLSSNWRATEELKSWLTRTGRIAIGGVD
EEECCCCCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCC
TRRLTRAIRQQGAPHVAMEHNPDGNFDLEALVAAARAWPGLEGMDLAKDVTCAQSYRWDE
HHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
MRWAWPEGYTRQEEPKHKVVAIDYGAKRNILRCLASAGCDVTVLPATATSEEVLAHGPDG
CEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCC
VFLSNGPGDPAATGAYAVPMIKEILDKTDLPVFGICLGHQMLALALGAKTTKMNHGHHGA
EEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCEECCCCCCCC
NHPVKEHGTGKVEITSMNHGFAVDAQTLPEGVEETHVSLFDGSNCGIRMTDRPVYSVQHH
CCCHHHCCCCEEEEEECCCCEEEEHHHCCCCHHHHEEEEEECCCCCEEECCCCEEEECCC
PEASPGPQDSFYLFERFAEAMAARKA
CCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVETVSPKPTACLALADGTVFYGHGFGATGRCVAELCFNTAMTGYQEIMTDPSYAGQVVT
CCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
FTFPHIGNTGVTPEDDETADPVAAGMVVKWDPTLSSNWRATEELKSWLTRTGRIAIGGVD
EEECCCCCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCC
TRRLTRAIRQQGAPHVAMEHNPDGNFDLEALVAAARAWPGLEGMDLAKDVTCAQSYRWDE
HHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
MRWAWPEGYTRQEEPKHKVVAIDYGAKRNILRCLASAGCDVTVLPATATSEEVLAHGPDG
CEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCC
VFLSNGPGDPAATGAYAVPMIKEILDKTDLPVFGICLGHQMLALALGAKTTKMNHGHHGA
EEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCEECCCCCCCC
NHPVKEHGTGKVEITSMNHGFAVDAQTLPEGVEETHVSLFDGSNCGIRMTDRPVYSVQHH
CCCHHHCCCCEEEEEECCCCEEEEHHHCCCCHHHHEEEEEECCCCCEEECCCCEEEECCC
PEASPGPQDSFYLFERFAEAMAARKA
CCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA