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The map label for this gene is gltX

Identifier: 99082115

GI number: 99082115

Start: 2390226

End: 2391551

Strand: Reverse

Name: gltX

Synonym: TM1040_2275

Alternate gene names: 99082115

Gene position: 2391551-2390226 (Counterclockwise)

Preceding gene: 99082117

Following gene: 99082112

Centisome position: 74.71

GC content: 63.12

Gene sequence:

>1326_bases
ATGACCACCACCCGTTTTGCGCCGTCCCCCACCGGCTATATCCATGTGGGCAACCTGCGCACTGCTTTGATGAACTACCT
GATCGCTCGCAAGGCGGGCGGCACCTTCATTCTGCGCATCGACGACACCGACCCCGAGCGCTCCAAGGAGGAATACGTCG
ACGCGATCAAGCAGGATCTCGAATGGCTCGGCATCACCTGGGACAAGGTGGAGCGCCAGTCCGAGCGGCTCGACCGCTAT
GCCGAGGCCGCCGACAAGCTGCGCGAGATCGGCCGGTTCTACGAGGCCTTCGAGACCCCGACCGAACTCGATCTCAAACG
CAAAAAGCAGCTCAACATGGGCAAGCCGCCGGTCTATGACCGCGCCGCACTGGCGCTGTCGGATGCGGAAAAAGAGTCCC
TGCGCGCCGAGCGCGGCAATGGTGTGTGGCGCTTCAAGCTCGATCAGGAACGCATCGAGTGGACCGATGGCATCCTTGGT
GACATCTCCATCGACGCGGCGTCTGTCTCGGACCCGGTGCTGATCCGCGGCGACGGGCAGGTCCTTTATACCATCGCCTC
TGTGGTGGACGACACCGACATGGGCGTGACCCATGTTGTGCGCGGCTCTGACCATGTCACCAATACCGCGACGCAAATTC
AGATCATGGCTGCGCTCGGCCATGGCCATCCCGAGTTTGCCCACCACTCGCTGCTGACCGGCCCGCAGGGCGAGGCGCTC
TCCAAGCGTCTTGGCACGTTGGCGCTGCGCGATCTGCGCGAGGCGGGCGTCAAACCGATGGCGCTTCTGTCGCTGATGGC
GCGTCTAGGGTCCTCTGATCCGGTGGAATTGCGCACCGATATGGCCGAGCTGGTTGACGGTTTTGACATCAACCGTTTCG
GCTCTGCGCCGACCAAGTTCGACGCCGAGGATCTCTATCCGCTGACCGCGCGCTACCTGCAGACCCTGCCGGTTGCGACA
GTCAAATCCGAGCTCGACGCCATTGGCGTGCCCGCAGACACCCAAGAGGCCTTTTGGGCGGTGGCCAAGGAAAACATCAC
CACGCTCAAGGATCTCGAGGGCTGGTGGATCTTGTGCCGCGACGGAGCGGAGCCGCTGATTGCCGACGAGGACAAGGAGT
TTATCGCCGAGGCGATGACCCTTCTGCCCGAAGGCCCCTACGACTCTGAGAGCTGGGGCAAATGGACCGCAGCAGTGAAG
GAAAAGACCGGCCGCAAAGGCAAGGGGCTCTTTATGCCGCTGCGCAAGGCCGTCACCGGCATGGAGCGTGGCCCGGATAT
GTCCGCACTTCTGGCCTTGATGCAGACCGTGCGCGCCCGCGGCTGA

Upstream 100 bases:

>100_bases
GAGGCCACGCCCCGCAGGGGCGCACGCAGAGGGGCACACCATGCGAAAACTGGACAATTCCGCGCGCCTGTGACAGAGCA
GGCGCAACAGGAGATTTGCG

Downstream 100 bases:

>100_bases
TTGAAACCACCGCGCCAGGCGCGGCGTGGATCACGTCACAATTTATGAACGGGCAGGGCCAAGGCGCTGCCCGTATTTTT
ATGAATTCTCAAAGTAGTCC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTTTRFAPSPTGYIHVGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDAIKQDLEWLGITWDKVERQSERLDRY
AEAADKLREIGRFYEAFETPTELDLKRKKQLNMGKPPVYDRAALALSDAEKESLRAERGNGVWRFKLDQERIEWTDGILG
DISIDAASVSDPVLIRGDGQVLYTIASVVDDTDMGVTHVVRGSDHVTNTATQIQIMAALGHGHPEFAHHSLLTGPQGEAL
SKRLGTLALRDLREAGVKPMALLSLMARLGSSDPVELRTDMAELVDGFDINRFGSAPTKFDAEDLYPLTARYLQTLPVAT
VKSELDAIGVPADTQEAFWAVAKENITTLKDLEGWWILCRDGAEPLIADEDKEFIAEAMTLLPEGPYDSESWGKWTAAVK
EKTGRKGKGLFMPLRKAVTGMERGPDMSALLALMQTVRARG

Sequences:

>Translated_441_residues
MTTTRFAPSPTGYIHVGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDAIKQDLEWLGITWDKVERQSERLDRY
AEAADKLREIGRFYEAFETPTELDLKRKKQLNMGKPPVYDRAALALSDAEKESLRAERGNGVWRFKLDQERIEWTDGILG
DISIDAASVSDPVLIRGDGQVLYTIASVVDDTDMGVTHVVRGSDHVTNTATQIQIMAALGHGHPEFAHHSLLTGPQGEAL
SKRLGTLALRDLREAGVKPMALLSLMARLGSSDPVELRTDMAELVDGFDINRFGSAPTKFDAEDLYPLTARYLQTLPVAT
VKSELDAIGVPADTQEAFWAVAKENITTLKDLEGWWILCRDGAEPLIADEDKEFIAEAMTLLPEGPYDSESWGKWTAAVK
EKTGRKGKGLFMPLRKAVTGMERGPDMSALLALMQTVRARG
>Mature_440_residues
TTTRFAPSPTGYIHVGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDAIKQDLEWLGITWDKVERQSERLDRYA
EAADKLREIGRFYEAFETPTELDLKRKKQLNMGKPPVYDRAALALSDAEKESLRAERGNGVWRFKLDQERIEWTDGILGD
ISIDAASVSDPVLIRGDGQVLYTIASVVDDTDMGVTHVVRGSDHVTNTATQIQIMAALGHGHPEFAHHSLLTGPQGEALS
KRLGTLALRDLREAGVKPMALLSLMARLGSSDPVELRTDMAELVDGFDINRFGSAPTKFDAEDLYPLTARYLQTLPVATV
KSELDAIGVPADTQEAFWAVAKENITTLKDLEGWWILCRDGAEPLIADEDKEFIAEAMTLLPEGPYDSESWGKWTAAVKE
KTGRKGKGLFMPLRKAVTGMERGPDMSALLALMQTVRARG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=324, Percent_Identity=36.1111111111111, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI62241042, Length=373, Percent_Identity=24.9329758713137, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1788743, Length=347, Percent_Identity=36.5994236311239, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=34.412955465587, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786895, Length=211, Percent_Identity=29.8578199052133, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71993394, Length=466, Percent_Identity=30.2575107296137, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=343, Percent_Identity=34.9854227405248, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321192, Length=101, Percent_Identity=37.6237623762376, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=129, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=474, Percent_Identity=30.5907172995781, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_SILST (Q1GEA9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614269.1
- ProteinModelPortal:   Q1GEA9
- SMR:   Q1GEA9
- STRING:   Q1GEA9
- GeneID:   4078459
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2275
- NMPDR:   fig|292414.1.peg.637
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BioCyc:   SSP292414:TM1040_2275-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 48893; Mature: 48762

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTRFAPSPTGYIHVGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDAIKQDL
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
EWLGITWDKVERQSERLDRYAEAADKLREIGRFYEAFETPTELDLKRKKQLNMGKPPVYD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
RAALALSDAEKESLRAERGNGVWRFKLDQERIEWTDGILGDISIDAASVSDPVLIRGDGQ
HHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCEEECCCCCCCCEEEECCCC
VLYTIASVVDDTDMGVTHVVRGSDHVTNTATQIQIMAALGHGHPEFAHHSLLTGPQGEAL
EEHHHHHHHCCCCCCCEEEECCCCCCCCHHHHEEEEEHHCCCCHHHHHHHHCCCCCCHHH
SKRLGTLALRDLREAGVKPMALLSLMARLGSSDPVELRTDMAELVDGFDINRFGSAPTKF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DAEDLYPLTARYLQTLPVATVKSELDAIGVPADTQEAFWAVAKENITTLKDLEGWWILCR
CHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEEEC
DGAEPLIADEDKEFIAEAMTLLPEGPYDSESWGKWTAAVKEKTGRKGKGLFMPLRKAVTG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
MERGPDMSALLALMQTVRARG
CCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTTRFAPSPTGYIHVGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDAIKQDL
CCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
EWLGITWDKVERQSERLDRYAEAADKLREIGRFYEAFETPTELDLKRKKQLNMGKPPVYD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
RAALALSDAEKESLRAERGNGVWRFKLDQERIEWTDGILGDISIDAASVSDPVLIRGDGQ
HHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCEEECCCCCCCCEEEECCCC
VLYTIASVVDDTDMGVTHVVRGSDHVTNTATQIQIMAALGHGHPEFAHHSLLTGPQGEAL
EEHHHHHHHCCCCCCCEEEECCCCCCCCHHHHEEEEEHHCCCCHHHHHHHHCCCCCCHHH
SKRLGTLALRDLREAGVKPMALLSLMARLGSSDPVELRTDMAELVDGFDINRFGSAPTKF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DAEDLYPLTARYLQTLPVATVKSELDAIGVPADTQEAFWAVAKENITTLKDLEGWWILCR
CHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEEEC
DGAEPLIADEDKEFIAEAMTLLPEGPYDSESWGKWTAAVKEKTGRKGKGLFMPLRKAVTG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
MERGPDMSALLALMQTVRARG
CCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA