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The map label for this gene is gcvA [H]

Identifier: 99082032

GI number: 99082032

Start: 2302522

End: 2303400

Strand: Reverse

Name: gcvA [H]

Synonym: TM1040_2192

Alternate gene names: 99082032

Gene position: 2303400-2302522 (Counterclockwise)

Preceding gene: 99082035

Following gene: 99082030

Centisome position: 71.96

GC content: 57.0

Gene sequence:

>879_bases
ATGACCGAACTTCCCCCGCTCAACAGTTTGCGTGCTTTTGATGCAGCAGGCCGCCGCCTCAGTTTCCGTGCAGCCGCTGA
TGAGTTGGGTGTCACGCAAGGGGCCGTTGCGCAGCAGGTGCGACAACTCGAAGCGCATTTTGATGTGGTCTTGTTTGAAC
GGGTGCCGAAGGGCCTGGCTTTTACGCCAGTCGGGCGCAGCTATCACAACCGGATTGCCGGAGTGTTCGCAGACCTTCGA
GCCGCAACTGCCGAGTTGAAGCCAGAGCCGAATAAAGTTCTCATCAGTGTCACGCCAACCTTTGCTGCGAAATGGCTGAT
TCCCAATCTTCCCGACTTCGCTACAGCACATCCGAACATCGATTTGCGGATCATGGCGACCGAAAAAGTTTCCAGCTTCC
ATAGTGACGGGATAGACCTTGCAGTGCGCCAAGGATCGCCGCCGTTTGGCGCATCACTGGACGTAACCCTTTTATTCAAG
CAATCGCTAGTCGCGGTCGCCGCGCAAAAGCTTGCGGACAAGGGAGGCACTCCTCTTGATCCAGAAACCCTTGCCAAACA
GCCGAAGGTCCATGACGCCCATGACCTATGGCCCAGCTATCTGGCGCATTTGGGCGTACAGGACCAGTCTGCTCGGCACC
TTCGTCTGAGCCAGACGTCTCTTGCGGTAGATGCAGCTATCGCAGGGCAAGGAGTGGCCTTGGTAAGCCGGTTTCTGGTT
GCTCATGACCTTGCAGCCGGACGTTTGGTCGAGGTTGGCGAAGCCTGGGCCCCAGACAGAAATGACTTCTATGTCCTCAT
GCCGCGAACCGCCAAAAACAATGCGCCGACGACCAACGTCTTGACGTGGCTCAAGTCGCGCTCCGCCAAAGATACCTGA

Upstream 100 bases:

>100_bases
ATATCGATCATCCTTTCTTGCTTGAGGCATAAATCGTGCATTTTTTGGCAAACTTGGATTCAGAATTGATGTTATATGAC
AGTAGAATTACTATTACAGA

Downstream 100 bases:

>100_bases
TGCCTTGAAACAAAGCGGCCATTTAGATCACTCGGACGAACGGAAACAAGGTCCCAATCTCAGACAGACAGGTTTGCCTC
GCGTTTGACAGTTGGGCACT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTELPPLNSLRAFDAAGRRLSFRAAADELGVTQGAVAQQVRQLEAHFDVVLFERVPKGLAFTPVGRSYHNRIAGVFADLR
AATAELKPEPNKVLISVTPTFAAKWLIPNLPDFATAHPNIDLRIMATEKVSSFHSDGIDLAVRQGSPPFGASLDVTLLFK
QSLVAVAAQKLADKGGTPLDPETLAKQPKVHDAHDLWPSYLAHLGVQDQSARHLRLSQTSLAVDAAIAGQGVALVSRFLV
AHDLAAGRLVEVGEAWAPDRNDFYVLMPRTAKNNAPTTNVLTWLKSRSAKDT

Sequences:

>Translated_292_residues
MTELPPLNSLRAFDAAGRRLSFRAAADELGVTQGAVAQQVRQLEAHFDVVLFERVPKGLAFTPVGRSYHNRIAGVFADLR
AATAELKPEPNKVLISVTPTFAAKWLIPNLPDFATAHPNIDLRIMATEKVSSFHSDGIDLAVRQGSPPFGASLDVTLLFK
QSLVAVAAQKLADKGGTPLDPETLAKQPKVHDAHDLWPSYLAHLGVQDQSARHLRLSQTSLAVDAAIAGQGVALVSRFLV
AHDLAAGRLVEVGEAWAPDRNDFYVLMPRTAKNNAPTTNVLTWLKSRSAKDT
>Mature_291_residues
TELPPLNSLRAFDAAGRRLSFRAAADELGVTQGAVAQQVRQLEAHFDVVLFERVPKGLAFTPVGRSYHNRIAGVFADLRA
ATAELKPEPNKVLISVTPTFAAKWLIPNLPDFATAHPNIDLRIMATEKVSSFHSDGIDLAVRQGSPPFGASLDVTLLFKQ
SLVAVAAQKLADKGGTPLDPETLAKQPKVHDAHDLWPSYLAHLGVQDQSARHLRLSQTSLAVDAAIAGQGVALVSRFLVA
HDLAAGRLVEVGEAWAPDRNDFYVLMPRTAKNNAPTTNVLTWLKSRSAKDT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=35.1535836177474, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786448, Length=247, Percent_Identity=31.1740890688259, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=23.6486486486486, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788706, Length=254, Percent_Identity=26.3779527559055, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786401, Length=247, Percent_Identity=22.6720647773279, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787128, Length=306, Percent_Identity=21.8954248366013, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87081978, Length=282, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=164, Percent_Identity=32.3170731707317, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31558; Mature: 31427

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELPPLNSLRAFDAAGRRLSFRAAADELGVTQGAVAQQVRQLEAHFDVVLFERVPKGLA
CCCCCCCCHHHHHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHCCCCCE
FTPVGRSYHNRIAGVFADLRAATAELKPEPNKVLISVTPTFAAKWLIPNLPDFATAHPNI
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCC
DLRIMATEKVSSFHSDGIDLAVRQGSPPFGASLDVTLLFKQSLVAVAAQKLADKGGTPLD
EEEEEEHHHHHHHCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCC
PETLAKQPKVHDAHDLWPSYLAHLGVQDQSARHLRLSQTSLAVDAAIAGQGVALVSRFLV
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHH
AHDLAAGRLVEVGEAWAPDRNDFYVLMPRTAKNNAPTTNVLTWLKSRSAKDT
HHHHHCCCEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TELPPLNSLRAFDAAGRRLSFRAAADELGVTQGAVAQQVRQLEAHFDVVLFERVPKGLA
CCCCCCCHHHHHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHCCCCCE
FTPVGRSYHNRIAGVFADLRAATAELKPEPNKVLISVTPTFAAKWLIPNLPDFATAHPNI
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCC
DLRIMATEKVSSFHSDGIDLAVRQGSPPFGASLDVTLLFKQSLVAVAAQKLADKGGTPLD
EEEEEEHHHHHHHCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCC
PETLAKQPKVHDAHDLWPSYLAHLGVQDQSARHLRLSQTSLAVDAAIAGQGVALVSRFLV
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHH
AHDLAAGRLVEVGEAWAPDRNDFYVLMPRTAKNNAPTTNVLTWLKSRSAKDT
HHHHHCCCEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]