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The map label for this gene is betT [C]

Identifier: 99081965

GI number: 99081965

Start: 2230638

End: 2232287

Strand: Reverse

Name: betT [C]

Synonym: TM1040_2125

Alternate gene names: 99081965

Gene position: 2232287-2230638 (Counterclockwise)

Preceding gene: 99081966

Following gene: 99081963

Centisome position: 69.74

GC content: 59.58

Gene sequence:

>1650_bases
ATGACCGACCAAACGACGGAAACGCCCGACGGCATCCCAAGCCCGGACGGCGCAGCACATATCATTGATACGGATTATGA
AATCGGGCAGGACAACGTCGAGGGGTCCGTTGGCCCCTTTGGCTTTGACATCCACAACCCGGTGTTTGCCATCTCCGGGA
TCGCGGTTGTGGCCTTCGTGTTCTACACGCTCGCACTGCCCGAGCAGGCCGGCAACGTGTTCTCCTGGCTCTTTAGCGCA
GTGACCAAGGGCTTTGACTGGTTCTTCCTCGGTGCCGCAAATATCTTTGTGATCTTCTGCCTGTTCCTGATCGTGACGCC
CTTTGGCAATGTGAGGCTCGGCGGCACGGAGGCAGAACCCGACTATAGCTACATTGGCTGGTTTGCGATGCTGTTTGCAG
CCGGTATGGGCATCGGTTTGATGTTCTATGGGGTTTCCGAACCTCTGAGCCATTTTGCCTCTTCCATCGGGGGCACCGCA
TCCGAGGGCGGCGTGCGTACCGACTGGGCACCACTTGGGGCCGCAGGTGGTAATGAAGCCGAGGCCGTGCGCCTCGGGAT
GGCGGCGACGATCTTTCACTGGGGGCTTCACCCTTGGGCGATCTATGCCGTTGTGGCGCTTGCGCTGGCCTTGTTCAGCT
ACAACAAGGGCCTGCCGCTCACGATCCGGTCTGCTTTCTATCCGATTTTTGGCGATGCCGTCTGGGGTTGGGTTGGCCAT
GTGATCGACATCCTTGCTGTCTTTGCGACCCTCTTTGGCCTGGCAACTTCGCTCGGATTTGGCGCAACGCAAGCCAATGC
GGGCCTCAATGAGCTGTTTGGCATCTCGATCGGGTCCACCACTGAGGTCATCCTGATCTCGGCCATCACCGCAATCGCAC
TGGTTTCTGTGCTGCGCGGCCTTGATGGCGGTGTGAAGATCCTGTCGGAGATCAATATGGGTCTGGCCTTTGTGCTGCTG
GTCTTTGTGCTGCTGGTGGGGCCGACAGTGTTCCTGCTGTCGCTGTTCTGGGACTCGCTGATGGCCTATTTCCAGTATCT
CCCGGCGCTCTCGAACCCGGTCGGACGTGAGGATGTCAACTTCATGCAGGGCTGGACGTCGTTCTACTGGGCGTGGTGGA
TTTCCTGGTCGCCGTTCGTGGGCATGTTCATCGCCCGCGTCAGCCGTGGCCGGACCGTGCGTGAATTCATCATCTGCGTG
CTGTTGATCCCGTCGATGGTCTGCGTCCTTTGGATGAGCGTATTCGGCGGCACTGCGATCCATCAGGTTCTCTCTGATGG
CTACACCGTAGCGCAGGATGCGGCGCTGGAGCTGAAGCTCTTCAAGATGCTGGATGTGATGCCGCTTGCCGGGATCACCT
CGCTGGTCGGCATCGTGCTGGTGATCGTGTTCTTCGTGACCTCCTCGGACTCGGGGTCGCTGGTGATCGACACCATCACC
GCTGGCGGCAAGGTAGATGCGCCGGTACCGCAGCGGGCCTTCTGGTGCGTCTTTGAAGGTGCGGTTGCGATCGTTCTCCT
CCTGAGCGCCGGCGGCCTGCAATCGTTGCAGTCCATGGTGATCTCTACGGGCCTGCCGTTCACGGTTGTGCTGTTGGTGA
TGTGCTATGCAATCCTGCGCGGCCTGATGAGCGAGCGAAAGGCGACCTAA

Upstream 100 bases:

>100_bases
GTCACGGGGCGCATGTTGCCGCCCATAGCGCCGCATCGGTGCTCTTGGTGCGTGATTAACGAATAACAGAACTGAAACTC
CGAAACGAAAGGCGGTAGTT

Downstream 100 bases:

>100_bases
GTGAGGGCCGCTGAGGCCCTCAGATCACGGAAAACAGAAACGGCGCCTCCCATGCGGGGGCGCCGTTTTGCATTTGGGTT
TGCGGATAGGGTCAGTCTGG

Product: choline/carnitine/betaine transport

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MTDQTTETPDGIPSPDGAAHIIDTDYEIGQDNVEGSVGPFGFDIHNPVFAISGIAVVAFVFYTLALPEQAGNVFSWLFSA
VTKGFDWFFLGAANIFVIFCLFLIVTPFGNVRLGGTEAEPDYSYIGWFAMLFAAGMGIGLMFYGVSEPLSHFASSIGGTA
SEGGVRTDWAPLGAAGGNEAEAVRLGMAATIFHWGLHPWAIYAVVALALALFSYNKGLPLTIRSAFYPIFGDAVWGWVGH
VIDILAVFATLFGLATSLGFGATQANAGLNELFGISIGSTTEVILISAITAIALVSVLRGLDGGVKILSEINMGLAFVLL
VFVLLVGPTVFLLSLFWDSLMAYFQYLPALSNPVGREDVNFMQGWTSFYWAWWISWSPFVGMFIARVSRGRTVREFIICV
LLIPSMVCVLWMSVFGGTAIHQVLSDGYTVAQDAALELKLFKMLDVMPLAGITSLVGIVLVIVFFVTSSDSGSLVIDTIT
AGGKVDAPVPQRAFWCVFEGAVAIVLLLSAGGLQSLQSMVISTGLPFTVVLLVMCYAILRGLMSERKAT

Sequences:

>Translated_549_residues
MTDQTTETPDGIPSPDGAAHIIDTDYEIGQDNVEGSVGPFGFDIHNPVFAISGIAVVAFVFYTLALPEQAGNVFSWLFSA
VTKGFDWFFLGAANIFVIFCLFLIVTPFGNVRLGGTEAEPDYSYIGWFAMLFAAGMGIGLMFYGVSEPLSHFASSIGGTA
SEGGVRTDWAPLGAAGGNEAEAVRLGMAATIFHWGLHPWAIYAVVALALALFSYNKGLPLTIRSAFYPIFGDAVWGWVGH
VIDILAVFATLFGLATSLGFGATQANAGLNELFGISIGSTTEVILISAITAIALVSVLRGLDGGVKILSEINMGLAFVLL
VFVLLVGPTVFLLSLFWDSLMAYFQYLPALSNPVGREDVNFMQGWTSFYWAWWISWSPFVGMFIARVSRGRTVREFIICV
LLIPSMVCVLWMSVFGGTAIHQVLSDGYTVAQDAALELKLFKMLDVMPLAGITSLVGIVLVIVFFVTSSDSGSLVIDTIT
AGGKVDAPVPQRAFWCVFEGAVAIVLLLSAGGLQSLQSMVISTGLPFTVVLLVMCYAILRGLMSERKAT
>Mature_548_residues
TDQTTETPDGIPSPDGAAHIIDTDYEIGQDNVEGSVGPFGFDIHNPVFAISGIAVVAFVFYTLALPEQAGNVFSWLFSAV
TKGFDWFFLGAANIFVIFCLFLIVTPFGNVRLGGTEAEPDYSYIGWFAMLFAAGMGIGLMFYGVSEPLSHFASSIGGTAS
EGGVRTDWAPLGAAGGNEAEAVRLGMAATIFHWGLHPWAIYAVVALALALFSYNKGLPLTIRSAFYPIFGDAVWGWVGHV
IDILAVFATLFGLATSLGFGATQANAGLNELFGISIGSTTEVILISAITAIALVSVLRGLDGGVKILSEINMGLAFVLLV
FVLLVGPTVFLLSLFWDSLMAYFQYLPALSNPVGREDVNFMQGWTSFYWAWWISWSPFVGMFIARVSRGRTVREFIICVL
LIPSMVCVLWMSVFGGTAIHQVLSDGYTVAQDAALELKLFKMLDVMPLAGITSLVGIVLVIVFFVTSSDSGSLVIDTITA
GGKVDAPVPQRAFWCVFEGAVAIVLLLSAGGLQSLQSMVISTGLPFTVVLLVMCYAILRGLMSERKAT

Specific function: High-Affinity Uptake Of Choline Driven By A Proton- Motive Force. [C]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=482, Percent_Identity=38.7966804979253, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1788102, Length=458, Percent_Identity=28.1659388646288, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786224, Length=493, Percent_Identity=27.1805273833671, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 58761; Mature: 58630

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQTTETPDGIPSPDGAAHIIDTDYEIGQDNVEGSVGPFGFDIHNPVFAISGIAVVAFV
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FYTLALPEQAGNVFSWLFSAVTKGFDWFFLGAANIFVIFCLFLIVTPFGNVRLGGTEAEP
HHHHHCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
DYSYIGWFAMLFAAGMGIGLMFYGVSEPLSHFASSIGGTASEGGVRTDWAPLGAAGGNEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
EAVRLGMAATIFHWGLHPWAIYAVVALALALFSYNKGLPLTIRSAFYPIFGDAVWGWVGH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHH
VIDILAVFATLFGLATSLGFGATQANAGLNELFGISIGSTTEVILISAITAIALVSVLRG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
LDGGVKILSEINMGLAFVLLVFVLLVGPTVFLLSLFWDSLMAYFQYLPALSNPVGREDVN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
FMQGWTSFYWAWWISWSPFVGMFIARVSRGRTVREFIICVLLIPSMVCVLWMSVFGGTAI
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
HQVLSDGYTVAQDAALELKLFKMLDVMPLAGITSLVGIVLVIVFFVTSSDSGSLVIDTIT
HHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
AGGKVDAPVPQRAFWCVFEGAVAIVLLLSAGGLQSLQSMVISTGLPFTVVLLVMCYAILR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GLMSERKAT
HHHHHCCCC
>Mature Secondary Structure 
TDQTTETPDGIPSPDGAAHIIDTDYEIGQDNVEGSVGPFGFDIHNPVFAISGIAVVAFV
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FYTLALPEQAGNVFSWLFSAVTKGFDWFFLGAANIFVIFCLFLIVTPFGNVRLGGTEAEP
HHHHHCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
DYSYIGWFAMLFAAGMGIGLMFYGVSEPLSHFASSIGGTASEGGVRTDWAPLGAAGGNEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
EAVRLGMAATIFHWGLHPWAIYAVVALALALFSYNKGLPLTIRSAFYPIFGDAVWGWVGH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHH
VIDILAVFATLFGLATSLGFGATQANAGLNELFGISIGSTTEVILISAITAIALVSVLRG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
LDGGVKILSEINMGLAFVLLVFVLLVGPTVFLLSLFWDSLMAYFQYLPALSNPVGREDVN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
FMQGWTSFYWAWWISWSPFVGMFIARVSRGRTVREFIICVLLIPSMVCVLWMSVFGGTAI
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
HQVLSDGYTVAQDAALELKLFKMLDVMPLAGITSLVGIVLVIVFFVTSSDSGSLVIDTIT
HHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
AGGKVDAPVPQRAFWCVFEGAVAIVLLLSAGGLQSLQSMVISTGLPFTVVLLVMCYAILR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GLMSERKAT
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]