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The map label for this gene is thiC

Identifier: 99081894

GI number: 99081894

Start: 2156958

End: 2158784

Strand: Direct

Name: thiC

Synonym: TM1040_2054

Alternate gene names: 99081894

Gene position: 2156958-2158784 (Clockwise)

Preceding gene: 99081892

Following gene: 99081898

Centisome position: 67.39

GC content: 61.85

Gene sequence:

>1827_bases
ATGAACACCCCAACCCGACACCCGACCCCCAACGTCACAACGGGCGCCCTGCCCGCCTCCCGTAAGATCTATGTGGCAGG
CGAGATGCATGAAGGCATCCGCGTGCCGATGCGCGAAATCGCCACCCACCCGACTGCGGGCGAGGCGCCGCTACCGGTTT
ATGACAGCTCCGGCCCCTATACCGACCCCGATGTGTCAACCGACATCCGCGAGGGATTGGCCCCGTTGCGCGCGGCGTGG
ATCAAGGCCCGCTGCGATGTTGAGGCCTATAGTGGCCGCGATGTGACCGCTGCAGACAATGGCTTTGTCGAAGGCGATCG
GCTGGTGCCTGAGTTTCCTGTAAAACCGGCGCCCCTGCGCGGGAGGCAGCGGGCCGCTCCGACTCAGCTGGCCTATGCGC
GCGCAGGAATCATTACGCCCGAGATGGAATTCGTGGCGATCCGAGAAAACCAATTACGCGATCTGTCGCCCTGTCATTGT
GCGCGCGACGGGGAGGATTTTGGCGCCAATATCCCCGACTACGTGACGCCAGAGTTTGTCCGCGCTGAAATCGCCGCAGG
GCGCGCCATCATCCCCGCCAATATCAACCACCCGGAGCTGGAGCCGATGATCATCGGCCGCAATTTCAAGGTGAAGATCA
ACGCCAATATGGGCACCTCTTCGGTGACCTCCTCGATGGAGGAGGAAGTGGACAAGCTGGTCTGGGCGATCCGCTGGGGC
GCAGATACGGTGATGGATCTCTCGACGGGCCGCAACATCCACAACACCCGCGAATGGATCATCCGCAACAGCCCGGTGCC
CATCGGCACCGTGCCGATCTATCAGGCGCTGGAGAAGGTGAACGGCATCGCCGAGGATCTGACATGGGAGGTGTTTCGCG
ACACGCTGATCGAGCAGGCGGAACAGGGCGTCGATTACTTTACCATTCATGCGGGCGTCCGGCTGCATATGGTGCCGATG
ACCGTGGAACGCGTGACGGGGATCGTGTCGCGCGGTGGGTCGATCATGGCGAAATGGTGCCTGCATCACCATCGCGAGAG
CTTCCTCTATGAGCACTTCGAGGAGATCTGCGACATCTGTCGGCAGTATGACGTGAGCTTCTCGCTGGGTGATGGCTTGC
GTCCCGGGTCGATTGCCGATGCCAATGACGCCGCGCAATTTGCCGAGCTGGAAACGCTGGGCGAGCTCACAAAAATCGCC
TGGGCCAAGGACTGTCAGGTGATGATCGAGGGGCCGGGCCATGTGGCGATGCACAAGATCAAGGAGAACATGGACAAGCA
GCTGGAGTGCTGCCACGAGGCGCCGTTTTACACGCTCGGCCCGCTCACCACGGATATTGCGCCGGGGTACGATCACATCA
CCTCCGGGATTGGCGCGGCGATGATCGGCTGGTTTGGCTGCGCGATGCTGTGTTACGTGACGCCCAAGGAACATCTGGGC
CTGCCGGACCGCGATGACGTTAAAACCGGGGTGATCACCTACAAGATCGCCGCCCATGCCGCTGATCTGGCCAAGGGTCT
GCCCGGCGCGCAGCGGCGCGATGATGCGCTGAGCCGCGCGCGGTTTGAATTCCGCTGGGAGGATCAGTTCAACCTCTCGC
TCGACCCGGAGACCGCGCAGAGCTTCCACGACGAGACCCTGCCCAAGGAGGCCCACAAGGTCGCGCATTTCTGTTCCATG
TGCGGGCCAAAGTTCTGCTCCATGCGGATCAGCCACGACATCCGCGCCGAAGCCCAGAAAGAAGGGTTTGAGGCGATGGC
GGCAAAATTCCGCGAAGGCGGTGAACTCTATGTACCACTGAAGGACACCGCACCAGAAGAGGCCTGA

Upstream 100 bases:

>100_bases
TTCGCGCTGATCTGAGACGGCAGGATCTGAACACCCTGCCCCGTTTTCCTGAGGCCCGGCTCAATTGGTGTTTTTGAGAA
TGGACTCGAGGAGCACCAAG

Downstream 100 bases:

>100_bases
GCGCAGCCGTGGCCACCCGCTGCGCTGCCTGTGCGCCTCTTTAGGCGCGCGGGCAGCGCCCGGCCCAACGGGAGGTCGGG
TCCCACCTTGAAAAGGTGGG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MNTPTRHPTPNVTTGALPASRKIYVAGEMHEGIRVPMREIATHPTAGEAPLPVYDSSGPYTDPDVSTDIREGLAPLRAAW
IKARCDVEAYSGRDVTAADNGFVEGDRLVPEFPVKPAPLRGRQRAAPTQLAYARAGIITPEMEFVAIRENQLRDLSPCHC
ARDGEDFGANIPDYVTPEFVRAEIAAGRAIIPANINHPELEPMIIGRNFKVKINANMGTSSVTSSMEEEVDKLVWAIRWG
ADTVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLHMVPM
TVERVTGIVSRGGSIMAKWCLHHHRESFLYEHFEEICDICRQYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIA
WAKDCQVMIEGPGHVAMHKIKENMDKQLECCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLG
LPDRDDVKTGVITYKIAAHAADLAKGLPGAQRRDDALSRARFEFRWEDQFNLSLDPETAQSFHDETLPKEAHKVAHFCSM
CGPKFCSMRISHDIRAEAQKEGFEAMAAKFREGGELYVPLKDTAPEEA

Sequences:

>Translated_608_residues
MNTPTRHPTPNVTTGALPASRKIYVAGEMHEGIRVPMREIATHPTAGEAPLPVYDSSGPYTDPDVSTDIREGLAPLRAAW
IKARCDVEAYSGRDVTAADNGFVEGDRLVPEFPVKPAPLRGRQRAAPTQLAYARAGIITPEMEFVAIRENQLRDLSPCHC
ARDGEDFGANIPDYVTPEFVRAEIAAGRAIIPANINHPELEPMIIGRNFKVKINANMGTSSVTSSMEEEVDKLVWAIRWG
ADTVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLHMVPM
TVERVTGIVSRGGSIMAKWCLHHHRESFLYEHFEEICDICRQYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIA
WAKDCQVMIEGPGHVAMHKIKENMDKQLECCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLG
LPDRDDVKTGVITYKIAAHAADLAKGLPGAQRRDDALSRARFEFRWEDQFNLSLDPETAQSFHDETLPKEAHKVAHFCSM
CGPKFCSMRISHDIRAEAQKEGFEAMAAKFREGGELYVPLKDTAPEEA
>Mature_608_residues
MNTPTRHPTPNVTTGALPASRKIYVAGEMHEGIRVPMREIATHPTAGEAPLPVYDSSGPYTDPDVSTDIREGLAPLRAAW
IKARCDVEAYSGRDVTAADNGFVEGDRLVPEFPVKPAPLRGRQRAAPTQLAYARAGIITPEMEFVAIRENQLRDLSPCHC
ARDGEDFGANIPDYVTPEFVRAEIAAGRAIIPANINHPELEPMIIGRNFKVKINANMGTSSVTSSMEEEVDKLVWAIRWG
ADTVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLHMVPM
TVERVTGIVSRGGSIMAKWCLHHHRESFLYEHFEEICDICRQYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIA
WAKDCQVMIEGPGHVAMHKIKENMDKQLECCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLG
LPDRDDVKTGVITYKIAAHAADLAKGLPGAQRRDDALSRARFEFRWEDQFNLSLDPETAQSFHDETLPKEAHKVAHFCSM
CGPKFCSMRISHDIRAEAQKEGFEAMAAKFREGGELYVPLKDTAPEEA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=607, Percent_Identity=68.5337726523888, Blast_Score=833, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_SILST (Q1GEY0)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614048.1
- STRING:   Q1GEY0
- GeneID:   4077981
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2054
- NMPDR:   fig|292414.1.peg.425
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   HLRFEAG
- PhylomeDB:   Q1GEY0
- ProtClustDB:   PRK09284
- BioCyc:   SSP292414:TM1040_2054-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 67291; Mature: 67291

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: BINDING 216-216 BINDING 245-245 BINDING 274-274 BINDING 310-310 BINDING 410-410 BINDING 437-437

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPTRHPTPNVTTGALPASRKIYVAGEMHEGIRVPMREIATHPTAGEAPLPVYDSSGPY
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCEECCCCCC
TDPDVSTDIREGLAPLRAAWIKARCDVEAYSGRDVTAADNGFVEGDRLVPEFPVKPAPLR
CCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
GRQRAAPTQLAYARAGIITPEMEFVAIRENQLRDLSPCHCARDGEDFGANIPDYVTPEFV
CCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHH
RAEIAAGRAIIPANINHPELEPMIIGRNFKVKINANMGTSSVTSSMEEEVDKLVWAIRWG
HHHHHCCCEEEECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
ADTVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLIEQA
CCCEEECCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGVDYFTIHAGVRLHMVPMTVERVTGIVSRGGSIMAKWCLHHHRESFLYEHFEEICDIC
HCCCCEEEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKDCQVMIEGPGHVAMHKI
HHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
KENMDKQLECCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLG
HHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHCC
LPDRDDVKTGVITYKIAAHAADLAKGLPGAQRRDDALSRARFEFRWEDQFNLSLDPETAQ
CCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCEEEEEECCCCCEEECCHHHH
SFHDETLPKEAHKVAHFCSMCGPKFCSMRISHDIRAEAQKEGFEAMAAKFREGGELYVPL
HHHHHCCHHHHHHHHHHHHHCCHHHHHEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
KDTAPEEA
CCCCCCCC
>Mature Secondary Structure
MNTPTRHPTPNVTTGALPASRKIYVAGEMHEGIRVPMREIATHPTAGEAPLPVYDSSGPY
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCEECCCCCC
TDPDVSTDIREGLAPLRAAWIKARCDVEAYSGRDVTAADNGFVEGDRLVPEFPVKPAPLR
CCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
GRQRAAPTQLAYARAGIITPEMEFVAIRENQLRDLSPCHCARDGEDFGANIPDYVTPEFV
CCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHH
RAEIAAGRAIIPANINHPELEPMIIGRNFKVKINANMGTSSVTSSMEEEVDKLVWAIRWG
HHHHHCCCEEEECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
ADTVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLIEQA
CCCEEECCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGVDYFTIHAGVRLHMVPMTVERVTGIVSRGGSIMAKWCLHHHRESFLYEHFEEICDIC
HCCCCEEEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKDCQVMIEGPGHVAMHKI
HHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
KENMDKQLECCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLG
HHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHCC
LPDRDDVKTGVITYKIAAHAADLAKGLPGAQRRDDALSRARFEFRWEDQFNLSLDPETAQ
CCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCEEEEEECCCCCEEECCHHHH
SFHDETLPKEAHKVAHFCSMCGPKFCSMRISHDIRAEAQKEGFEAMAAKFREGGELYVPL
HHHHHCCHHHHHHHHHHHHHCCHHHHHEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
KDTAPEEA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA