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The map label for this gene is rlmB [H]

Identifier: 99081889

GI number: 99081889

Start: 2153152

End: 2153940

Strand: Direct

Name: rlmB [H]

Synonym: TM1040_2049

Alternate gene names: 99081889

Gene position: 2153152-2153940 (Clockwise)

Preceding gene: 99081888

Following gene: 99081890

Centisome position: 67.27

GC content: 59.19

Gene sequence:

>789_bases
ATGAACAAGAAATCCAACAAGAAACCCGCTTGGGTCGTGGAGAAGGAAAAGGCCAAAAAGGCGGCGGCATCCGAGACCGT
CTGGCTCTTTGGTCTGCATGCGGTGCGGGATGCGCTTTTGAACCCCAAACGTGAGAAGCTACGCCTTGTGGTGACGCTCA
ATGCGCAGAACAAACTGGAGGATGCCATTGCGACCTCTGGCATCACGCCCGAGGTGGTGGACCCGCGCAAATTCTCGGTA
CCGATTGATCCGGGCTCTGTACATCAGGGCGCTGCACTCGAGGTCAAGCCTCTGAACTGGGGCGGGCTTGAGGACAATTG
TATCGGGGCCGAAGTGCCGCGCGTGATCCTTCTGGACCGGGTAACTGATCCGCACAATGTCGGCGCGATCCTGCGCTCTG
CAGAGGTGCTGGGTGCATCGGCGGTGATCGGCACCCGGCATCACTCCGCACCGGAAACCGGTGCGCTCGCCAAGACGGCA
TCGGGCGCGCTTGAACGTCAGCCCTATCTCAGAATGCGCAATCTCTCCGATACCATCGTGGCACTGCAGCAGATGGGGTA
TCTGGTGCTTGGCCTTGATGGCGAGGCAGAAGAGACCATCGAGGAGGCGGTCGCCGGGCGCACCAAAGAGCCGATCGCGC
TGGTCCTGGGCGCCGAAGGTCCCGGTCTGCGACAAAAAACCAAGGAAACCGTGGACAAGCTTGTCAAAATCGATGCCGCG
CGCGGTTTTGGCTCCTTGAATGTTTCAAATGCCGCTGCACTCTCCCTATATGCATCTTTGAAGGGTTAG

Upstream 100 bases:

>100_bases
ACCTGACGTGGAGGCGTGATTACTAGCGCACCAAACGGGCGTCAACCGGAAAAACGCAACTTTTTTCCGCTGCCTTGCAT
TTTGTTTTCCGGAGCATGCC

Downstream 100 bases:

>100_bases
GACGCAGAGGAGGCTCCCATCGCACATCACAGTATTCCCGGAGGCAAGCTGATTGTCTGTTGTTATTGTGGCGTGCAATC
CGCCTTTGTGCCGGACGCCA

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNKKSNKKPAWVVEKEKAKKAAASETVWLFGLHAVRDALLNPKREKLRLVVTLNAQNKLEDAIATSGITPEVVDPRKFSV
PIDPGSVHQGAALEVKPLNWGGLEDNCIGAEVPRVILLDRVTDPHNVGAILRSAEVLGASAVIGTRHHSAPETGALAKTA
SGALERQPYLRMRNLSDTIVALQQMGYLVLGLDGEAEETIEEAVAGRTKEPIALVLGAEGPGLRQKTKETVDKLVKIDAA
RGFGSLNVSNAAALSLYASLKG

Sequences:

>Translated_262_residues
MNKKSNKKPAWVVEKEKAKKAAASETVWLFGLHAVRDALLNPKREKLRLVVTLNAQNKLEDAIATSGITPEVVDPRKFSV
PIDPGSVHQGAALEVKPLNWGGLEDNCIGAEVPRVILLDRVTDPHNVGAILRSAEVLGASAVIGTRHHSAPETGALAKTA
SGALERQPYLRMRNLSDTIVALQQMGYLVLGLDGEAEETIEEAVAGRTKEPIALVLGAEGPGLRQKTKETVDKLVKIDAA
RGFGSLNVSNAAALSLYASLKG
>Mature_262_residues
MNKKSNKKPAWVVEKEKAKKAAASETVWLFGLHAVRDALLNPKREKLRLVVTLNAQNKLEDAIATSGITPEVVDPRKFSV
PIDPGSVHQGAALEVKPLNWGGLEDNCIGAEVPRVILLDRVTDPHNVGAILRSAEVLGASAVIGTRHHSAPETGALAKTA
SGALERQPYLRMRNLSDTIVALQQMGYLVLGLDGEAEETIEEAVAGRTKEPIALVLGAEGPGLRQKTKETVDKLVKIDAA
RGFGSLNVSNAAALSLYASLKG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=188, Percent_Identity=34.0425531914894, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=35.3909465020576, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17555908, Length=270, Percent_Identity=28.5185185185185, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=284, Percent_Identity=29.2253521126761, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27912; Mature: 27912

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSNKKPAWVVEKEKAKKAAASETVWLFGLHAVRDALLNPKREKLRLVVTLNAQNKLE
CCCCCCCCCCEEEEHHHHHHHHCCCEEHEHHHHHHHHHHCCCCCCEEEEEEEECCCHHHH
DAIATSGITPEVVDPRKFSVPIDPGSVHQGAALEVKPLNWGGLEDNCIGAEVPRVILLDR
HHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEC
VTDPHNVGAILRSAEVLGASAVIGTRHHSAPETGALAKTASGALERQPYLRMRNLSDTIV
CCCCHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCHHHHHCCHHHCCCHHHHHCCHHHHH
ALQQMGYLVLGLDGEAEETIEEAVAGRTKEPIALVLGAEGPGLRQKTKETVDKLVKIDAA
HHHHCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
RGFGSLNVSNAAALSLYASLKG
CCCCCCCCCCHHHEEEEEECCC
>Mature Secondary Structure
MNKKSNKKPAWVVEKEKAKKAAASETVWLFGLHAVRDALLNPKREKLRLVVTLNAQNKLE
CCCCCCCCCCEEEEHHHHHHHHCCCEEHEHHHHHHHHHHCCCCCCEEEEEEEECCCHHHH
DAIATSGITPEVVDPRKFSVPIDPGSVHQGAALEVKPLNWGGLEDNCIGAEVPRVILLDR
HHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEC
VTDPHNVGAILRSAEVLGASAVIGTRHHSAPETGALAKTASGALERQPYLRMRNLSDTIV
CCCCHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCHHHHHCCHHHCCCHHHHHCCHHHHH
ALQQMGYLVLGLDGEAEETIEEAVAGRTKEPIALVLGAEGPGLRQKTKETVDKLVKIDAA
HHHHCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
RGFGSLNVSNAAALSLYASLKG
CCCCCCCCCCHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]