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The map label for this gene is gcvA [H]

Identifier: 99081844

GI number: 99081844

Start: 2109545

End: 2110426

Strand: Direct

Name: gcvA [H]

Synonym: TM1040_2004

Alternate gene names: 99081844

Gene position: 2109545-2110426 (Clockwise)

Preceding gene: 99081843

Following gene: 99081846

Centisome position: 65.9

GC content: 64.29

Gene sequence:

>882_bases
ATGCGCAGGCTGCCCCATGTCACCTGGCTTCGCTCCTTCGAGGCCGCCGCCCGTCACAGTTCCTTCAGCGCCGCAGCCGA
AGAGTTGCACCTGACCCCCGCCGCAGTGAGCCAGCAAATCCGGCAGCTCGAACACCACCTCGGGTTTCAGCTCTTCAAAC
GCCTGCCCAAAGGGGTAGAGCTGACCGACATTGGCCAGGCCTATGCGATCCCGGTGCGCAAGTCGTTTGTGCAGATGCAA
GAAGCCACGGATGGGCTGTTTCGGGTTCAGAGCAAATCCGTGCTGCGCGTGCGCTGCTCGATCAGCTTTGCGGCGCTGAT
CCTGGCACCGCTCCTGCGCGACTTCGCCGAAATCCATCCCGACATCGACGTCGAACTCTCGACCGCAGTTTGGTCGGATC
GGATGGAGGGCGCCGGGGTGGATCTCGATATTCGCTACGGCGCCGCCTGCTGGCCGGAGCAAAACGTCCACCCGCTTCCG
GTCGAAGAGGCCACGTTGGTCTGCCACCCGGACTATGCCGCGCAGCTTGGCAGCGCGCTTGATCCGGCATGGCTCGGGGC
AGCGGACATTGTGCAGATCGTGGGCTCCGAGACCGATTGGGACCGCTATCTGCGGCTCTGCGGGATCGATACGCCGCGCC
CGCGACACTGGATGAAGGCCGATTCCTCGCTGATCGCCCTGCAGATCCTGTCGGCGGGGCGCGGCTGCGCCCTGATTTCG
CGCAGTTTCGCCCGTCAGGCGCTCAGCGAGGGACGCCTGGTTGCGCCTTTTGATCTGAGCCTGCCGATGCAGGAGAGCTT
TGTTCTGGTCGAGCGCGACGACCTGAGGGAAAACCCGGGTCTGCGCAAACTTGTGGACTGGCTGCTGACGCAGAGGCTCT
AG

Upstream 100 bases:

>100_bases
CCTTGTGCCAGTCGACCGAGTTGATTTCTGTTGTCTCATTTGGGCTTGTCGCGTGTCAGGCGCGCGCCCATGATGGGCGC
AAACAACAACCGGAGCCCCC

Downstream 100 bases:

>100_bases
ACGGTCGCGGCCGCCTTGCGGACCCAATCGACAAACAGAAGCGTGTCCTGCGAGGGCGTCGTGGGCATGGTGACAAAATA
GCTCGTCCCGAGATCCAGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRRLPHVTWLRSFEAAARHSSFSAAAEELHLTPAAVSQQIRQLEHHLGFQLFKRLPKGVELTDIGQAYAIPVRKSFVQMQ
EATDGLFRVQSKSVLRVRCSISFAALILAPLLRDFAEIHPDIDVELSTAVWSDRMEGAGVDLDIRYGAACWPEQNVHPLP
VEEATLVCHPDYAAQLGSALDPAWLGAADIVQIVGSETDWDRYLRLCGIDTPRPRHWMKADSSLIALQILSAGRGCALIS
RSFARQALSEGRLVAPFDLSLPMQESFVLVERDDLRENPGLRKLVDWLLTQRL

Sequences:

>Translated_293_residues
MRRLPHVTWLRSFEAAARHSSFSAAAEELHLTPAAVSQQIRQLEHHLGFQLFKRLPKGVELTDIGQAYAIPVRKSFVQMQ
EATDGLFRVQSKSVLRVRCSISFAALILAPLLRDFAEIHPDIDVELSTAVWSDRMEGAGVDLDIRYGAACWPEQNVHPLP
VEEATLVCHPDYAAQLGSALDPAWLGAADIVQIVGSETDWDRYLRLCGIDTPRPRHWMKADSSLIALQILSAGRGCALIS
RSFARQALSEGRLVAPFDLSLPMQESFVLVERDDLRENPGLRKLVDWLLTQRL
>Mature_293_residues
MRRLPHVTWLRSFEAAARHSSFSAAAEELHLTPAAVSQQIRQLEHHLGFQLFKRLPKGVELTDIGQAYAIPVRKSFVQMQ
EATDGLFRVQSKSVLRVRCSISFAALILAPLLRDFAEIHPDIDVELSTAVWSDRMEGAGVDLDIRYGAACWPEQNVHPLP
VEEATLVCHPDYAAQLGSALDPAWLGAADIVQIVGSETDWDRYLRLCGIDTPRPRHWMKADSSLIALQILSAGRGCALIS
RSFARQALSEGRLVAPFDLSLPMQESFVLVERDDLRENPGLRKLVDWLLTQRL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=34.4827586206897, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786448, Length=251, Percent_Identity=32.2709163346614, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=28.3737024221453, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=31.1188811188811, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787128, Length=176, Percent_Identity=30.6818181818182, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=173, Percent_Identity=30.635838150289, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=22.8668941979522, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789639, Length=138, Percent_Identity=28.2608695652174, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87081978, Length=181, Percent_Identity=30.3867403314917, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=254, Percent_Identity=25.9842519685039, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789440, Length=155, Percent_Identity=28.3870967741935, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32766; Mature: 32766

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLPHVTWLRSFEAAARHSSFSAAAEELHLTPAAVSQQIRQLEHHLGFQLFKRLPKGVE
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LTDIGQAYAIPVRKSFVQMQEATDGLFRVQSKSVLRVRCSISFAALILAPLLRDFAEIHP
EEECCCCEEHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
DIDVELSTAVWSDRMEGAGVDLDIRYGAACWPEQNVHPLPVEEATLVCHPDYAAQLGSAL
CCCEEEHHHHHHHCCCCCCCEEEECCCCEECCCCCCCCCCCCCCEEEECCCHHHHHCCCC
DPAWLGAADIVQIVGSETDWDRYLRLCGIDTPRPRHWMKADSSLIALQILSAGRGCALIS
CHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCCCCEEEHHHHHCCCCHHHHH
RSFARQALSEGRLVAPFDLSLPMQESFVLVERDDLRENPGLRKLVDWLLTQRL
HHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRRLPHVTWLRSFEAAARHSSFSAAAEELHLTPAAVSQQIRQLEHHLGFQLFKRLPKGVE
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LTDIGQAYAIPVRKSFVQMQEATDGLFRVQSKSVLRVRCSISFAALILAPLLRDFAEIHP
EEECCCCEEHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
DIDVELSTAVWSDRMEGAGVDLDIRYGAACWPEQNVHPLPVEEATLVCHPDYAAQLGSAL
CCCEEEHHHHHHHCCCCCCCEEEECCCCEECCCCCCCCCCCCCCEEEECCCHHHHHCCCC
DPAWLGAADIVQIVGSETDWDRYLRLCGIDTPRPRHWMKADSSLIALQILSAGRGCALIS
CHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCCCCEEEHHHHHCCCCHHHHH
RSFARQALSEGRLVAPFDLSLPMQESFVLVERDDLRENPGLRKLVDWLLTQRL
HHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]