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The map label for this gene is pyrG [H]

Identifier: 99081813

GI number: 99081813

Start: 2077611

End: 2079254

Strand: Direct

Name: pyrG [H]

Synonym: TM1040_1973

Alternate gene names: 99081813

Gene position: 2077611-2079254 (Clockwise)

Preceding gene: 99081807

Following gene: 99081814

Centisome position: 64.91

GC content: 60.64

Gene sequence:

>1644_bases
ATGGCGCGTTTCATCTTTATTACTGGCGGTGTCGTCTCTTCTCTGGGCAAGGGACTGGCCTCTGCTGCGCTGGGTGCGCT
GCTGCAGGCACGGGGCTACTCCGTGCGCCTGCGCAAACTCGACCCCTATCTGAACGTCGATCCGGGCACCATGAGCCCGT
TTGAACACGGCGAGGTCTTTGTGACCGACGACGGGGCAGAAACCGACCTCGATCTGGGCCACTACGAGCGCTTCACCGGC
GTGCCAGCGCGTAAGACCGACTCGATCTCCTCCGGGCGGATCTACACCAACGTGCTCGAGAAAGAGCGCCGCGGCGACTA
CCTCGGCAAAACGATCCAGGTGATTCCGCATGTCACCAACGAGATCAAGGACTTCATCTCCATCGGAGAGGATGAGGTCG
ATTTCATGCTCTGCGAGATCGGCGGCACCGTGGGTGACATCGAGGGGCTGCCCTTCTTTGAGGCCATCCGCCAGTTCAGC
CAGGACAAGCCGCGCGGTCAGTGTATCTTCATGCACCTGACGCTGCTTCCCTACATCAAGGCCTCTGGCGAGTTGAAAAC
CAAACCGACCCAGCACTCCGTGAAGGAGCTGCGTTCCATCGGTCTGGCCCCCGATATTCTGGTCTGCCGCTCCGAGGGTC
CGATCCCGGTGAAAGAACGCGAAAAGCTGGCGCTCTTCTGCAACGTGCGCGCCGACAGCGTGATTGCGGCGCAGGATCTG
AAATCCATCTACGAAGCGCCGCTGGCCTATCACCGCGAAGGACTCGATCAGGCGGTTCTGGACGCCTTTGGCATCGCCCC
TGCCCCGCGCCCGACGCTGGACACCTGGGAAGACGTGGCCGACCGCATCTACAACCCCGAGGGCGAAGTCAAAGTCGCCA
TCGTCGGCAAATACACCCAGCTGGAAGACGCCTATAAATCCATCGCCGAGGCGCTCACCCATGGTGGCATGGCGAACCGG
GTAAAGGTGCGTATCGAATGGGTCGATGCAGAGCTCTTCGACAAGGAAGACGCAACGCCGTATCTGCAGGGTTTTCACGC
GATCCTCGTGCCCGGCGGCTTTGGCGAGCGCGGCACCGAAGGCAAGATCAAGGCGGCGCAATATGCCCGCGAGCACAAGG
TGCCCTACCTCGGTATCTGCCTCGGTATGCAGATGGCCGTGATCGAAGCTGCCCGCAACGTGGCCGGCATCGAGGAAGCA
GGCTCCGAAGAGTTCGACCATGAAGCGGGCAAAAAGCGTTTTGAGCCCGTTGTTTACCACCTCAAGGAATGGGTGCAGGG
CAACCACAAGGTGAGCCGCAGCGCCGATGACGACAAAGGCGGCACCATGCGTCTTGGCGCCTATGATGCGGCGCTTACCG
AAGGTTCCAAAGTGGCCGAGGTCTATGGCTCCAACGCGATTGAGGAGCGTCACCGCCACCGCTATGAGGTGGACATCAAG
TACCGCGAGCAACTCGAAGCCTGTGGTCTGAAGTTCACTGGCATGAGCCCGGATGGCCGCCTGCCCGAGATCGTGGAATG
GACCGATCATCCGTGGTTTATCGGCGTGCAGTTCCACCCGGAACTCAAGTCCAAGCCCTTTGATCCGCACCCGCTGTTCA
AGGATTTTGTACGCGCGGCCAAGGACACATCCCGCTTGGTCTAA

Upstream 100 bases:

>100_bases
CGCTGATCTTACCGCCGGGCAGCAAGAGATGCGGGGTCTTCCCCGGATCACGACTGAACCGATTTGGACCTGACACGATC
TCACGGGGGCAGCCGAAAAC

Downstream 100 bases:

>100_bases
GCCCTGCGCATATCACCCAAAAAATCAGGCGCGTCCCTCGGGGCGCGCCTGTTTCGTTAGCAATTTATCAGCCGGTTTCT
CTTATGCTCCTGAGTCGAAG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MARFIFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTG
VPARKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTNEIKDFISIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFS
QDKPRGQCIFMHLTLLPYIKASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPVKEREKLALFCNVRADSVIAAQDL
KSIYEAPLAYHREGLDQAVLDAFGIAPAPRPTLDTWEDVADRIYNPEGEVKVAIVGKYTQLEDAYKSIAEALTHGGMANR
VKVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTEGKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEA
GSEEFDHEAGKKRFEPVVYHLKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIK
YREQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFVRAAKDTSRLV

Sequences:

>Translated_547_residues
MARFIFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTG
VPARKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTNEIKDFISIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFS
QDKPRGQCIFMHLTLLPYIKASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPVKEREKLALFCNVRADSVIAAQDL
KSIYEAPLAYHREGLDQAVLDAFGIAPAPRPTLDTWEDVADRIYNPEGEVKVAIVGKYTQLEDAYKSIAEALTHGGMANR
VKVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTEGKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEA
GSEEFDHEAGKKRFEPVVYHLKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIK
YREQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFVRAAKDTSRLV
>Mature_546_residues
ARFIFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGV
PARKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTNEIKDFISIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFSQ
DKPRGQCIFMHLTLLPYIKASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPVKEREKLALFCNVRADSVIAAQDLK
SIYEAPLAYHREGLDQAVLDAFGIAPAPRPTLDTWEDVADRIYNPEGEVKVAIVGKYTQLEDAYKSIAEALTHGGMANRV
KVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTEGKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEAG
SEEFDHEAGKKRFEPVVYHLKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIKY
REQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFVRAAKDTSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=43.0357142857143, Blast_Score=464, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=564, Percent_Identity=41.6666666666667, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI28559083, Length=564, Percent_Identity=41.6666666666667, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI221316689, Length=564, Percent_Identity=41.6666666666667, Blast_Score=453, Evalue=1e-127,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=54.4280442804428, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI25148299, Length=611, Percent_Identity=36.4975450081833, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=40.7732864674868, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319432, Length=572, Percent_Identity=40.5594405594406, Blast_Score=422, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=562, Percent_Identity=43.2384341637011, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI21357815, Length=508, Percent_Identity=42.5196850393701, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60781; Mature: 60649

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFIFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVPARKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
EIKDFISIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFSQDKPRGQCIFMHLTLLPYIK
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHH
ASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPVKEREKLALFCNVRADSVIAAQDL
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
KSIYEAPLAYHREGLDQAVLDAFGIAPAPRPTLDTWEDVADRIYNPEGEVKVAIVGKYTQ
HHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCHH
LEDAYKSIAEALTHGGMANRVKVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTE
HHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHCCCCCCCHHHCCCEEEEECCCCCCCCCC
GKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEAGSEEFDHEAGKKRFEPVVYH
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
LKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIK
HHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCHHHHHHCCCHHHHHHCCCEEEEEH
YREQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFVRAA
HHHHHHHCCCEEECCCCCCCCCHHHCCCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHH
KDTSRLV
HHHHCCC
>Mature Secondary Structure 
ARFIFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVPARKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
EIKDFISIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFSQDKPRGQCIFMHLTLLPYIK
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHH
ASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPVKEREKLALFCNVRADSVIAAQDL
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
KSIYEAPLAYHREGLDQAVLDAFGIAPAPRPTLDTWEDVADRIYNPEGEVKVAIVGKYTQ
HHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCHH
LEDAYKSIAEALTHGGMANRVKVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTE
HHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHCCCCCCCHHHCCCEEEEECCCCCCCCCC
GKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEAGSEEFDHEAGKKRFEPVVYH
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
LKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIK
HHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCHHHHHHCCCHHHHHHCCCEEEEEH
YREQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFVRAA
HHHHHHHCCCEEECCCCCCCCCHHHCCCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHH
KDTSRLV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA