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The map label for this gene is purA

Identifier: 99081809

GI number: 99081809

Start: 2073570

End: 2074883

Strand: Reverse

Name: purA

Synonym: TM1040_1969

Alternate gene names: 99081809

Gene position: 2074883-2073570 (Counterclockwise)

Preceding gene: 99081810

Following gene: 99081808

Centisome position: 64.82

GC content: 63.09

Gene sequence:

>1314_bases
ATGGCCAATGTCGTGGTAGTCGGCGCCCAGTGGGGCGATGAAGGTAAAGGCAAGATCGTGGATTGGCTCAGCGAGCGCGC
CGATGTCATCGCGCGCTTCCAAGGCGGCCACAACGCCGGCCATACGCTTGTCATCGACAAGAAGGTCTACAAGCTGCACG
CGCTACCCTCCGGCGTTGTGCGCGGGGGCAAGCTCTCCGTGATTGGCAACGGTGTGGTGCTGGACCCCTGGCACCTGATG
AAAGAGATCGAGACCGTGCGCGCACAGGGTGTGGAGATCACGCCCGAGACGCTGATGATCGCGGAAAACACGCCGCTGAT
CATGCCGTTCCACGGTGAACTGGACCGTGCCCGTGAAGAGGCCGCCTCCAAGGGCACCAAGATCGGCACCACCGGTCGCG
GCATTGGCCCGGCCTATGAGGACAAAGTGGGTCGTCGCGCCATCCGTGTTGCGGATCTCGCTGATGACGCCACGCTTGAG
GCGCGCGTCGACCGCGCGCTGCAGCATCACGATCCGCTGCGCAAAGGTCTCGGCGTGGAGCCGATCGACCGCGACGCGCT
GGTGGCACAGCTGAAAGAGATCGCCTCCGAGATCCTGCCCTTCGCAGCACCCGTCTGGAAGGTGATGAACGAGAAGCGCA
AAGCAGGCAAACGCATCCTCTTTGAGGGCGCACAGGGCGCGCTTCTGGACATTGATTTTGGCACCTATCCCTTTGTGACC
TCGTCCAATGTGATCGCAGGCCAAGCCGCCACCGGTGTTGGCATTGGCCCCAACTCGATCGACTATGTGCTCGGCATCGT
CAAAGCCTATACCACCCGCGTGGGTGAGGGCCCGTTCCCGACGGAACTGCTGGGCGCGGATGGCAAACCCGATGCCGATG
GCGAGCGTCTGGGCACGCGCGGCCATGAGTTTGGCACCACAACCGGCCGTCAGCGCCGCTGCGGCTGGTTTGACGCCTGC
CTCGTACGCCAGACCTGCGCGACCTCCGGCATCAACGGTATTTCGCTGACCAAGCTCGACGTTCTCGACGGGTTTGAAAC
CCTGAAAATCTGCGTCGGCTACGAGCTCGACGGTGAACGTCTCGATTACCTGCCCACGGCCGCAGACCAGCAGGCGCGGT
GCAAGCCCATCTATGAAGAGATGGAAGGCTGGAGCGAATCCACCGAGGGCGCGCGCAGCTGGGCAGAGCTGCCTGCCAAT
GCGATCAAATATGTGCGCCGCGTCGAGGAGCTGATCCAATGCCCGGTTGCCCTTCTGTCCACCAGCCCGGAGCGGGACGA
CACCATTCTGGTGACGGACCCGTTTGCTGACTGA

Upstream 100 bases:

>100_bases
GGCAGATTGTAACCGTCTTGCGACGAGATTCCGGTTTCCTCGCCCGCGCCGCCTCGCTATACGGGGCGTCGGTTTCAACC
CAGCATGCAAAGGACCTGAG

Downstream 100 bases:

>100_bases
TGGCCAAGGAAGGTCTGAGCTACCGCGCCCGCCGCATCTGGGCCCTGGTCATCCTCCTGGTCGGGATGCCGCTCTATATC
GTGCTGGCTGTGACCATCCT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGHNAGHTLVIDKKVYKLHALPSGVVRGGKLSVIGNGVVLDPWHLM
KEIETVRAQGVEITPETLMIAENTPLIMPFHGELDRAREEAASKGTKIGTTGRGIGPAYEDKVGRRAIRVADLADDATLE
ARVDRALQHHDPLRKGLGVEPIDRDALVAQLKEIASEILPFAAPVWKVMNEKRKAGKRILFEGAQGALLDIDFGTYPFVT
SSNVIAGQAATGVGIGPNSIDYVLGIVKAYTTRVGEGPFPTELLGADGKPDADGERLGTRGHEFGTTTGRQRRCGWFDAC
LVRQTCATSGINGISLTKLDVLDGFETLKICVGYELDGERLDYLPTAADQQARCKPIYEEMEGWSESTEGARSWAELPAN
AIKYVRRVEELIQCPVALLSTSPERDDTILVTDPFAD

Sequences:

>Translated_437_residues
MANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGHNAGHTLVIDKKVYKLHALPSGVVRGGKLSVIGNGVVLDPWHLM
KEIETVRAQGVEITPETLMIAENTPLIMPFHGELDRAREEAASKGTKIGTTGRGIGPAYEDKVGRRAIRVADLADDATLE
ARVDRALQHHDPLRKGLGVEPIDRDALVAQLKEIASEILPFAAPVWKVMNEKRKAGKRILFEGAQGALLDIDFGTYPFVT
SSNVIAGQAATGVGIGPNSIDYVLGIVKAYTTRVGEGPFPTELLGADGKPDADGERLGTRGHEFGTTTGRQRRCGWFDAC
LVRQTCATSGINGISLTKLDVLDGFETLKICVGYELDGERLDYLPTAADQQARCKPIYEEMEGWSESTEGARSWAELPAN
AIKYVRRVEELIQCPVALLSTSPERDDTILVTDPFAD
>Mature_436_residues
ANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGHNAGHTLVIDKKVYKLHALPSGVVRGGKLSVIGNGVVLDPWHLMK
EIETVRAQGVEITPETLMIAENTPLIMPFHGELDRAREEAASKGTKIGTTGRGIGPAYEDKVGRRAIRVADLADDATLEA
RVDRALQHHDPLRKGLGVEPIDRDALVAQLKEIASEILPFAAPVWKVMNEKRKAGKRILFEGAQGALLDIDFGTYPFVTS
SNVIAGQAATGVGIGPNSIDYVLGIVKAYTTRVGEGPFPTELLGADGKPDADGERLGTRGHEFGTTTGRQRRCGWFDACL
VRQTCATSGINGISLTKLDVLDGFETLKICVGYELDGERLDYLPTAADQQARCKPIYEEMEGWSESTEGARSWAELPANA
IKYVRRVEELIQCPVALLSTSPERDDTILVTDPFAD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=42.6573426573427, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=40.8352668213457, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI40316944, Length=400, Percent_Identity=40.5, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1790620, Length=436, Percent_Identity=49.7706422018349, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=43.5185185185185, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=43.5185185185185, Blast_Score=349, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=435, Percent_Identity=40.9195402298851, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI21356033, Length=429, Percent_Identity=42.6573426573427, Blast_Score=325, Evalue=4e-89,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_SILST (Q1GF65)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613963.1
- ProteinModelPortal:   Q1GF65
- SMR:   Q1GF65
- STRING:   Q1GF65
- GeneID:   4077153
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1969
- NMPDR:   fig|292414.1.peg.353
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q1GF65
- ProtClustDB:   PRK01117
- BioCyc:   SSP292414:TM1040_1969-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47336; Mature: 47205

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 131-131 BINDING 145-145 BINDING 225-225 BINDING 240-240 BINDING 310-310 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGHNAGHTLVIDKKVYKLHALPSGVV
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHEEECCCHHHC
RGGKLSVIGNGVVLDPWHLMKEIETVRAQGVEITPETLMIAENTPLIMPFHGELDRAREE
CCCEEEEEECCEEECHHHHHHHHHHHHHCCCEECCCEEEEECCCCEEEECCCCHHHHHHH
AASKGTKIGTTGRGIGPAYEDKVGRRAIRVADLADDATLEARVDRALQHHDPLRKGLGVE
HHHCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCHHHHCCCCC
PIDRDALVAQLKEIASEILPFAAPVWKVMNEKRKAGKRILFEGAQGALLDIDFGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCCEE
SSNVIAGQAATGVGIGPNSIDYVLGIVKAYTTRVGEGPFPTELLGADGKPDADGERLGTR
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHCCC
GHEFGTTTGRQRRCGWFDACLVRQTCATSGINGISLTKLDVLDGFETLKICVGYELDGER
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHEEEEEEEECCCCCC
LDYLPTAADQQARCKPIYEEMEGWSESTEGARSWAELPANAIKYVRRVEELIQCPVALLS
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHC
TSPERDDTILVTDPFAD
CCCCCCCEEEEECCCCC
>Mature Secondary Structure 
ANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGHNAGHTLVIDKKVYKLHALPSGVV
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHEEECCCHHHC
RGGKLSVIGNGVVLDPWHLMKEIETVRAQGVEITPETLMIAENTPLIMPFHGELDRAREE
CCCEEEEEECCEEECHHHHHHHHHHHHHCCCEECCCEEEEECCCCEEEECCCCHHHHHHH
AASKGTKIGTTGRGIGPAYEDKVGRRAIRVADLADDATLEARVDRALQHHDPLRKGLGVE
HHHCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCHHHHCCCCC
PIDRDALVAQLKEIASEILPFAAPVWKVMNEKRKAGKRILFEGAQGALLDIDFGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCCEE
SSNVIAGQAATGVGIGPNSIDYVLGIVKAYTTRVGEGPFPTELLGADGKPDADGERLGTR
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHCCC
GHEFGTTTGRQRRCGWFDACLVRQTCATSGINGISLTKLDVLDGFETLKICVGYELDGER
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHEEEEEEEECCCCCC
LDYLPTAADQQARCKPIYEEMEGWSESTEGARSWAELPANAIKYVRRVEELIQCPVALLS
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHC
TSPERDDTILVTDPFAD
CCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA