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The map label for this gene is metZ [H]

Identifier: 99081779

GI number: 99081779

Start: 2041410

End: 2042597

Strand: Reverse

Name: metZ [H]

Synonym: TM1040_1938

Alternate gene names: 99081779

Gene position: 2042597-2041410 (Counterclockwise)

Preceding gene: 99081781

Following gene: 99081778

Centisome position: 63.81

GC content: 59.76

Gene sequence:

>1188_bases
ATGACAGAGAGCTGGAACAAACGCACCAAACTGGTCCATGGCGGCACCCGACGCAGCCAGTACAACGAGGTCAGCGAAGC
GATCTTTCTGACCCAGGGGTTTGTGTACGACAGTGCAGAGCAGGCGGAGGCCCGGTTCATCGAGACCGGCGCGGATGAAT
TCATCTACGCCCGCTATGGCAATCCGACCGTGGCAATGTTTGAAGAGCGCATCGCCCTTTTAGAAGGGGCCGAGGATGCC
TTTGCCACCGCATCGGGCATGGCGGCGGTGAATGGCGCGCTCACGTCGATCCTGAAAGCGGGCGACCATGTGGTCTCTGC
CAAGGCGCTCTTTGGGTCCTGTCTTTATATTCTCGAAAACATCCTGACCCGGTATGGGGTCGAGGTGACATTTGTCGACG
GCACGGATCTGGACCAGTGGCGCGCTGCCGTGCGCCCGGACACCAAGGCCGTGTTTTTTGAGAGCATGTCGAACCCAACG
CTGGAAGTCATTGATATTGAAGGCGTTGCCGAGATCGCCCATGCGGTCGGCGCGACCGTTGTGGTCGATAATGTGTTCTC
TACTCCGGTGTTTTCCAATGCCATCGCGCAAGGCGCGGATGTGGTGGTGTATTCGGCCACCAAACATATCGACGGGCAGG
GGCGTGCGCTTGGCGGCGTGGTGCTTGGCACCAAGGATTACATTCGCGGCACGCTCGAACCCTACATGAAACATACCGGT
GGCTCGCTGAGCCCGTTTCATGCCTGGATGTTCGTCAAGGGGCTTGAGACCATTGATCTGCGCGTCAATGCTCAGGCTGC
CAGCGCGCTCAAGATCGCCGAGGCCTTTGAGAGCCATCCAGTCTTGGCTCGCACCATCTATCCGGGCCTGAAAACCCACG
CGCAGAATGCGCTGGTGCAGCGCCAGCTTGGAGGCAAGGGTGGGACGGTGCTGTCGCTCGACCTCAAGGGCGGCAAGGAG
GCGGCCTTCGCCTTTCTCAATGCGCTCTCCATTCCGGTGATCTCCAATAATCTGGGCGATGCCAAATCCATTGCCACCCA
TCCGGCCACCACCACGCACCAACGTCTTTCCGAGGCGCAGCGCGCAGAACTCGGGATCACCGATGGTCTGGTGCGGTTTT
CGGTCGGGCTTGAGGATGCGGATGATCTGATCGCGGATCTGTCACAGGCGCTGCAAACGCTGGCGTGA

Upstream 100 bases:

>100_bases
AAAGAGGTCAGGACGACGGGACTCCCTTTCGCTTGGCCGAACGGGAGTTTTTTTATGCGCGGCACGGCCCCGAGGGGCGC
TGACCGGCGGAGGCAAGACG

Downstream 100 bases:

>100_bases
CCCTGGAGCTTCGTGTGGCTCGCGCCCCGCGGGGCGCCGCCACATTTGGAGTTTGACATAAAATGTCCGGACCCACTTTG
GTGCAAAAGGTCAGATCAGT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTESWNKRTKLVHGGTRRSQYNEVSEAIFLTQGFVYDSAEQAEARFIETGADEFIYARYGNPTVAMFEERIALLEGAEDA
FATASGMAAVNGALTSILKAGDHVVSAKALFGSCLYILENILTRYGVEVTFVDGTDLDQWRAAVRPDTKAVFFESMSNPT
LEVIDIEGVAEIAHAVGATVVVDNVFSTPVFSNAIAQGADVVVYSATKHIDGQGRALGGVVLGTKDYIRGTLEPYMKHTG
GSLSPFHAWMFVKGLETIDLRVNAQAASALKIAEAFESHPVLARTIYPGLKTHAQNALVQRQLGGKGGTVLSLDLKGGKE
AAFAFLNALSIPVISNNLGDAKSIATHPATTTHQRLSEAQRAELGITDGLVRFSVGLEDADDLIADLSQALQTLA

Sequences:

>Translated_395_residues
MTESWNKRTKLVHGGTRRSQYNEVSEAIFLTQGFVYDSAEQAEARFIETGADEFIYARYGNPTVAMFEERIALLEGAEDA
FATASGMAAVNGALTSILKAGDHVVSAKALFGSCLYILENILTRYGVEVTFVDGTDLDQWRAAVRPDTKAVFFESMSNPT
LEVIDIEGVAEIAHAVGATVVVDNVFSTPVFSNAIAQGADVVVYSATKHIDGQGRALGGVVLGTKDYIRGTLEPYMKHTG
GSLSPFHAWMFVKGLETIDLRVNAQAASALKIAEAFESHPVLARTIYPGLKTHAQNALVQRQLGGKGGTVLSLDLKGGKE
AAFAFLNALSIPVISNNLGDAKSIATHPATTTHQRLSEAQRAELGITDGLVRFSVGLEDADDLIADLSQALQTLA
>Mature_394_residues
TESWNKRTKLVHGGTRRSQYNEVSEAIFLTQGFVYDSAEQAEARFIETGADEFIYARYGNPTVAMFEERIALLEGAEDAF
ATASGMAAVNGALTSILKAGDHVVSAKALFGSCLYILENILTRYGVEVTFVDGTDLDQWRAAVRPDTKAVFFESMSNPTL
EVIDIEGVAEIAHAVGATVVVDNVFSTPVFSNAIAQGADVVVYSATKHIDGQGRALGGVVLGTKDYIRGTLEPYMKHTGG
SLSPFHAWMFVKGLETIDLRVNAQAASALKIAEAFESHPVLARTIYPGLKTHAQNALVQRQLGGKGGTVLSLDLKGGKEA
AFAFLNALSIPVISNNLGDAKSIATHPATTTHQRLSEAQRAELGITDGLVRFSVGLEDADDLIADLSQALQTLA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=340, Percent_Identity=37.6470588235294, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI299473759, Length=340, Percent_Identity=34.1176470588235, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI25453487, Length=339, Percent_Identity=31.2684365781711, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1790375, Length=337, Percent_Identity=33.5311572700297, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1789383, Length=389, Percent_Identity=26.9922879177378, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17560056, Length=389, Percent_Identity=37.7892030848329, Blast_Score=233, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI17538055, Length=389, Percent_Identity=36.5038560411311, Blast_Score=215, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI71980708, Length=348, Percent_Identity=33.0459770114943, Blast_Score=194, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=401, Percent_Identity=36.6583541147132, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=30.3398058252427, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=26.044226044226, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=402, Percent_Identity=28.6069651741293, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=286, Percent_Identity=25.5244755244755, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=35.8247422680412, Blast_Score=237, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42211; Mature: 42080

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESWNKRTKLVHGGTRRSQYNEVSEAIFLTQGFVYDSAEQAEARFIETGADEFIYARYG
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHCCCCCEEEEEEC
NPTVAMFEERIALLEGAEDAFATASGMAAVNGALTSILKAGDHVVSAKALFGSCLYILEN
CCHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILTRYGVEVTFVDGTDLDQWRAAVRPDTKAVFFESMSNPTLEVIDIEGVAEIAHAVGATV
HHHHHCCEEEEECCCCHHHHHHHCCCCCCEEEECCCCCCCEEEEECCHHHHHHHHHCCCE
VVDNVFSTPVFSNAIAQGADVVVYSATKHIDGQGRALGGVVLGTKDYIRGTLEPYMKHTG
EECCHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCEECEEEECCHHHHHHHHHHHHHHCC
GSLSPFHAWMFVKGLETIDLRVNAQAASALKIAEAFESHPVLARTIYPGLKTHAQNALVQ
CCCCHHHHHHHHHCCEEEEEEEECHHHHHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHH
RQLGGKGGTVLSLDLKGGKEAAFAFLNALSIPVISNNLGDAKSIATHPATTTHQRLSEAQ
HHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCHHHHHHHHHH
RAELGITDGLVRFSVGLEDADDLIADLSQALQTLA
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TESWNKRTKLVHGGTRRSQYNEVSEAIFLTQGFVYDSAEQAEARFIETGADEFIYARYG
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHCCCCCEEEEEEC
NPTVAMFEERIALLEGAEDAFATASGMAAVNGALTSILKAGDHVVSAKALFGSCLYILEN
CCHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILTRYGVEVTFVDGTDLDQWRAAVRPDTKAVFFESMSNPTLEVIDIEGVAEIAHAVGATV
HHHHHCCEEEEECCCCHHHHHHHCCCCCCEEEECCCCCCCEEEEECCHHHHHHHHHCCCE
VVDNVFSTPVFSNAIAQGADVVVYSATKHIDGQGRALGGVVLGTKDYIRGTLEPYMKHTG
EECCHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCEECEEEECCHHHHHHHHHHHHHHCC
GSLSPFHAWMFVKGLETIDLRVNAQAASALKIAEAFESHPVLARTIYPGLKTHAQNALVQ
CCCCHHHHHHHHHCCEEEEEEEECHHHHHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHH
RQLGGKGGTVLSLDLKGGKEAAFAFLNALSIPVISNNLGDAKSIATHPATTTHQRLSEAQ
HHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCHHHHHHHHHH
RAELGITDGLVRFSVGLEDADDLIADLSQALQTLA
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]