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The map label for this gene is ilvB [H]

Identifier: 99081773

GI number: 99081773

Start: 2031373

End: 2033085

Strand: Reverse

Name: ilvB [H]

Synonym: TM1040_1932

Alternate gene names: 99081773

Gene position: 2033085-2031373 (Counterclockwise)

Preceding gene: 99081774

Following gene: 99081772

Centisome position: 63.52

GC content: 60.89

Gene sequence:

>1713_bases
ATGTCCAGCGACACAGCACAGCCCGCATCCGCCTCGCGTCGTAGCGGTGGCAGAGTTCTTGCAGATCAGTTGCAGATTCT
AGGGGCAGACACGATTTATTGCGTCCCCGGTGAGAGTTATCTGGAGTTGCTGGACGGGCTTCACGCCCACCAGGAGAGCA
TCAACGTGGTGACCTGTCGCCACGAATCCGGTGCCTCAAACATGGCCGATGCCTATGGTAAGCTGACCGGAAAGCCCGGC
ATCTGTGCTGTGACACGCGGGCCGGGAGCGACCAATGCCTCTAATGGGGTCCACACCGCGTTTCAGGACTCGACGCCTAT
GATCCTTCTGATCGGGCAGGTGGGGCGCGAGATGGTGGATCGCGAAGCGTTCCAAGAGATTGATTACCGGCGCATGTTTG
GCCAGATGGCCAAATGGGTAGCGCAGATCGATGATGCAGCCCGCATCCCAGAATATATGGCGCGCGCTTGGAAAACCGCG
CTTTCTGGTCGTCCCGGGCCGGTGGTTTTGGCGCTGCCAGAGGACATGCTGACCGATGAGGTCACGGTCGCAGATCTGCG
CGTGACGCCGCCCGCGCGTCCGGCCCCTGCCGCGCATGACATCGAGGCACTCTCGGCTATGATCGCCCGTGCCAAAAAGC
CGATGCTGATGGTAGGCGGGCCAAGCTGGAGCGAGGCCTGTGCAGACAAAGCGCTGGCCTTTGCCGAGCGTTTGGGCCTG
CCGGTCACGACATCCTTTCGCAGTCAGGACTATGTAAACAACGGTCACGACAATTATGTCGGCCCGGTTGGCATCGCCCC
AATTCCCAATCTTCGTAAACGGTTGCGTGAAGAGGTTGATCTTCTGATAACGGTTGGCCCGCGCCTTGGCGAGATGACGA
CCCAAGGCTACGAGCTCATCGATATTCCGCATCCGCAGATGACCTTTGTGCATGTGCATCCGGGTGCTGAGGAGTTGGGA
CAAGTCTATCAGCCCGATCTTGCGATCCAGTCCGACCCTGAGCTGTTCTTTGACGCCGCCGAGGCGATTACTCTGCCGAG
CCAGCCCGAAGGCTGGGCCGACTGGGTGCAGCAGCAGCGAGCGGATTACCTCGCCTTTCAGACCCCAACCGAGGTGCCAG
GGGATGTGAACATGGGCCATGTGGTGCGCCACATGAGCGAAACGCTGCCCGAGGACACGATCTACACCAACGGGGCGGGG
AACTATACAGTCTGGCTGCACCGGTTCCATCAGCACCGCGCCTATCGCACGCAACTGGCGCCCACCAGCGGCTCCATGGG
GTATGGTGTACCGGCGGCGGTTGCGGCCAAGCTCGCGCATCCCGACCGCACTGTGGTTGCGATGGCAGGCGATGGGTGTT
TCATGATGACCTCGCAAGAGCTGGCCACCGCGGCGCAGCATGGTGCTGATGTCATCTATGTTGTCGTAAACAATGGCATG
TACGGCACCATCCGCATGCATCAGGAACGCCATCACCCCGGCAAGGTCATGGCCACAAAGTTGGTGAACCCCGATTTTGT
CGCCTATGCCGCGTCCTTTGGGATCGCGGGGGAACGGGTGCTCCGCACCGAGGATTTCCCGGCGGCGTTGGAGCGCGCCC
GCACAACCAAGGGCGGCTACATGATCGAGATTGTGGTTGACCCCGAAGCCTTGACCCCAAACCAGTCGCTTTCGGCTGTA
CGCGCCGAAGGACAGGGCAAGGCAAACGCCTGA

Upstream 100 bases:

>100_bases
GACGAACTTTCCGTAAATTTGGCGAAACCACCTTCGGCGGGGCTAGGATTGAGCCCGCCAATGGATATGATCGCGCCGTT
CAGGAGAGGAAGACGAACAG

Downstream 100 bases:

>100_bases
GGCACGTTCTGCCTCTGGGTGCGCCAAACAATTCGTGACGGTGACTTGATTGACGCGGCCCCTCGGGTCGCGTCACTCTG
CGCTGCAGAAACGACTCGCG

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MSSDTAQPASASRRSGGRVLADQLQILGADTIYCVPGESYLELLDGLHAHQESINVVTCRHESGASNMADAYGKLTGKPG
ICAVTRGPGATNASNGVHTAFQDSTPMILLIGQVGREMVDREAFQEIDYRRMFGQMAKWVAQIDDAARIPEYMARAWKTA
LSGRPGPVVLALPEDMLTDEVTVADLRVTPPARPAPAAHDIEALSAMIARAKKPMLMVGGPSWSEACADKALAFAERLGL
PVTTSFRSQDYVNNGHDNYVGPVGIAPIPNLRKRLREEVDLLITVGPRLGEMTTQGYELIDIPHPQMTFVHVHPGAEELG
QVYQPDLAIQSDPELFFDAAEAITLPSQPEGWADWVQQQRADYLAFQTPTEVPGDVNMGHVVRHMSETLPEDTIYTNGAG
NYTVWLHRFHQHRAYRTQLAPTSGSMGYGVPAAVAAKLAHPDRTVVAMAGDGCFMMTSQELATAAQHGADVIYVVVNNGM
YGTIRMHQERHHPGKVMATKLVNPDFVAYAASFGIAGERVLRTEDFPAALERARTTKGGYMIEIVVDPEALTPNQSLSAV
RAEGQGKANA

Sequences:

>Translated_570_residues
MSSDTAQPASASRRSGGRVLADQLQILGADTIYCVPGESYLELLDGLHAHQESINVVTCRHESGASNMADAYGKLTGKPG
ICAVTRGPGATNASNGVHTAFQDSTPMILLIGQVGREMVDREAFQEIDYRRMFGQMAKWVAQIDDAARIPEYMARAWKTA
LSGRPGPVVLALPEDMLTDEVTVADLRVTPPARPAPAAHDIEALSAMIARAKKPMLMVGGPSWSEACADKALAFAERLGL
PVTTSFRSQDYVNNGHDNYVGPVGIAPIPNLRKRLREEVDLLITVGPRLGEMTTQGYELIDIPHPQMTFVHVHPGAEELG
QVYQPDLAIQSDPELFFDAAEAITLPSQPEGWADWVQQQRADYLAFQTPTEVPGDVNMGHVVRHMSETLPEDTIYTNGAG
NYTVWLHRFHQHRAYRTQLAPTSGSMGYGVPAAVAAKLAHPDRTVVAMAGDGCFMMTSQELATAAQHGADVIYVVVNNGM
YGTIRMHQERHHPGKVMATKLVNPDFVAYAASFGIAGERVLRTEDFPAALERARTTKGGYMIEIVVDPEALTPNQSLSAV
RAEGQGKANA
>Mature_569_residues
SSDTAQPASASRRSGGRVLADQLQILGADTIYCVPGESYLELLDGLHAHQESINVVTCRHESGASNMADAYGKLTGKPGI
CAVTRGPGATNASNGVHTAFQDSTPMILLIGQVGREMVDREAFQEIDYRRMFGQMAKWVAQIDDAARIPEYMARAWKTAL
SGRPGPVVLALPEDMLTDEVTVADLRVTPPARPAPAAHDIEALSAMIARAKKPMLMVGGPSWSEACADKALAFAERLGLP
VTTSFRSQDYVNNGHDNYVGPVGIAPIPNLRKRLREEVDLLITVGPRLGEMTTQGYELIDIPHPQMTFVHVHPGAEELGQ
VYQPDLAIQSDPELFFDAAEAITLPSQPEGWADWVQQQRADYLAFQTPTEVPGDVNMGHVVRHMSETLPEDTIYTNGAGN
YTVWLHRFHQHRAYRTQLAPTSGSMGYGVPAAVAAKLAHPDRTVVAMAGDGCFMMTSQELATAAQHGADVIYVVVNNGMY
GTIRMHQERHHPGKVMATKLVNPDFVAYAASFGIAGERVLRTEDFPAALERARTTKGGYMIEIVVDPEALTPNQSLSAVR
AEGQGKANA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=578, Percent_Identity=24.5674740484429, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI21361361, Length=568, Percent_Identity=26.7605633802817, Blast_Score=129, Evalue=9e-30,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=30.0546448087432, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=27.9646017699115, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786717, Length=549, Percent_Identity=27.5045537340619, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=24.3727598566308, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=24.6869409660107, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17542570, Length=570, Percent_Identity=24.2105263157895, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=573, Percent_Identity=24.78184991274, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=573, Percent_Identity=24.78184991274, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=28.6219081272085, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=559, Percent_Identity=22.0035778175313, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=489, Percent_Identity=22.2903885480573, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=507, Percent_Identity=22.2879684418146, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922626, Length=492, Percent_Identity=26.6260162601626, Blast_Score=132, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61745; Mature: 61613

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDTAQPASASRRSGGRVLADQLQILGADTIYCVPGESYLELLDGLHAHQESINVVTCR
CCCCCCCCCCHHHCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEE
HESGASNMADAYGKLTGKPGICAVTRGPGATNASNGVHTAFQDSTPMILLIGQVGREMVD
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEHHHHHHHH
REAFQEIDYRRMFGQMAKWVAQIDDAARIPEYMARAWKTALSGRPGPVVLALPEDMLTDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHCCCC
VTVADLRVTPPARPAPAAHDIEALSAMIARAKKPMLMVGGPSWSEACADKALAFAERLGL
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
PVTTSFRSQDYVNNGHDNYVGPVGIAPIPNLRKRLREEVDLLITVGPRLGEMTTQGYELI
CEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEE
DIPHPQMTFVHVHPGAEELGQVYQPDLAIQSDPELFFDAAEAITLPSQPEGWADWVQQQR
ECCCCCEEEEEECCCHHHHHHHHCCCCEECCCCHHHHCCHHHEECCCCCCHHHHHHHHHH
ADYLAFQTPTEVPGDVNMGHVVRHMSETLPEDTIYTNGAGNYTVWLHRFHQHRAYRTQLA
CCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHHHHCCC
PTSGSMGYGVPAAVAAKLAHPDRTVVAMAGDGCFMMTSQELATAAQHGADVIYVVVNNGM
CCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEEHHHHHHHHHCCCCEEEEEECCCE
YGTIRMHQERHHPGKVMATKLVNPDFVAYAASFGIAGERVLRTEDFPAALERARTTKGGY
EEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCEEECCCCHHHHHHHHCCCCCE
MIEIVVDPEALTPNQSLSAVRAEGQGKANA
EEEEEECCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSDTAQPASASRRSGGRVLADQLQILGADTIYCVPGESYLELLDGLHAHQESINVVTCR
CCCCCCCCCHHHCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEE
HESGASNMADAYGKLTGKPGICAVTRGPGATNASNGVHTAFQDSTPMILLIGQVGREMVD
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEHHHHHHHH
REAFQEIDYRRMFGQMAKWVAQIDDAARIPEYMARAWKTALSGRPGPVVLALPEDMLTDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHCCCC
VTVADLRVTPPARPAPAAHDIEALSAMIARAKKPMLMVGGPSWSEACADKALAFAERLGL
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
PVTTSFRSQDYVNNGHDNYVGPVGIAPIPNLRKRLREEVDLLITVGPRLGEMTTQGYELI
CEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEE
DIPHPQMTFVHVHPGAEELGQVYQPDLAIQSDPELFFDAAEAITLPSQPEGWADWVQQQR
ECCCCCEEEEEECCCHHHHHHHHCCCCEECCCCHHHHCCHHHEECCCCCCHHHHHHHHHH
ADYLAFQTPTEVPGDVNMGHVVRHMSETLPEDTIYTNGAGNYTVWLHRFHQHRAYRTQLA
CCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHHHHCCC
PTSGSMGYGVPAAVAAKLAHPDRTVVAMAGDGCFMMTSQELATAAQHGADVIYVVVNNGM
CCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEEHHHHHHHHHCCCCEEEEEECCCE
YGTIRMHQERHHPGKVMATKLVNPDFVAYAASFGIAGERVLRTEDFPAALERARTTKGGY
EEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCEEECCCCHHHHHHHHCCCCCE
MIEIVVDPEALTPNQSLSAVRAEGQGKANA
EEEEEECCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]