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The map label for this gene is ptsP [H]

Identifier: 99081774

GI number: 99081774

Start: 2033657

End: 2035897

Strand: Reverse

Name: ptsP [H]

Synonym: TM1040_1933

Alternate gene names: 99081774

Gene position: 2035897-2033657 (Counterclockwise)

Preceding gene: 99081775

Following gene: 99081773

Centisome position: 63.6

GC content: 59.3

Gene sequence:

>2241_bases
ATGGTTGAGAACACGGAATCCGAAAGCCGCAAGCTTCTGGGTCGTCTGCGCGAGGCCATGGCAGGGGATGACGCCGGACA
GGCGCGCCTCGACAAGATTACGCAGCTGATAGCCGACAGTATGGGCACCGAAGTGTGCTCTATCTATCTGTTTCGCGACG
AAGATACGCTCGAACTCTGCGCCACCGAAGGGCTCAACCGCGAGTCCGTGCACCAGACCCGCATGCGCGTGGGCGAGGGG
CTTGTGGGGCGTGTGGCGCGCACGGGCAAGGTGATCAACACCCCCGATGCGCCAAGCGCGCGGGGCTTTCGCTATATGCC
GGAAACCGGCGAGGAGCGCTTCTCCTCATTCCTGGGTATCCCCATCCAGCGCCTTGGCGAGATGATGGGCGTTCTTGTGG
TGCAATCCAAGGAAAGCCGCGAATATTCCGCGGACGCGGTCTATGCCCTTGAAGTTGTTGCAATGGTTATCGCCGAGATG
ACCGAACTTGGGGCCTTTGTGGGCGAGGGCTCTGCCATGTCGCCCCTGCACCAGCAATCTGTGCTCTTGAAGGGTATCGC
CGCGCAGGAGGGGGCCGCCGAGGGCAATATCTGGCTGCATGAGCCCCGCGTTGTGGTGACGAACCCGATTGCGGACGATC
CGCATCGGGAGCTGGAGCGCCTGCAGGAGGCCGTTGAGGAGCTGCGGGTGGGTGTCGACAAGATGCTGCAGGTCTCATCG
AACGGTGACAAGGAACAGGCACAGGTCCTTGAAACTTATCGCATGTTTGCCAACTCCAAAGGCTGGATGCGGCGCATGGA
AGAGGACATTGCCCGTGGCCTGAGCGCCGAAGCTGCAGTTGAAAAAGAACAATCCCTTGCAAGAGGCCGCATGGCGCAGG
TTCAGGACGCGTATTTGCGTGAGCGCCTGAGCGATCTGGACGATCTGTCGAACCGCCTGCTGCGTATTTTGACCGGTCAG
GGCAAGGATACCGGCGCAGAACTGCCGGAAAATCCCATCCTGGTCGCGCGCAATATCGGTCCGGCCGAATTGTTGGAATA
TGGTAGGTCTTTGAAGGGAATTATCCTTGAAGAGGGCTCTGTAGGTTCGCATGCGGCAATTGTAGCGCGGGCATTGGCGA
TCCCATTGGTGGTGCGTGTGGCCCGGATCACTACCGAAGGGCTGAATGGGGATCATGTTTTGGTGGACGGGGACAACGCT
GTTGTGCACCTGCGCCCCGATGATCCTGTCGTCAGCGCCTTTCGCGATAAGATTGCGATGCAAGCGAAGGCCCAGGAACG
GTATTCGTCGATACGTGAGAAACCTGCGATAACGGTCGATGGAAAAAGAATTAACCTGATGATGAACGCAGGGTTGATGG
CAGATCTGCCTTCGCTCGAGAATTCTGGCGCAGAGGGGGTTGGTCTGTTTCGCACTGAGCTGCAGTTCCTGGTGCGCAAT
CAGATGCCCAAACGCTCCGAGCTCAAGGGGCTCTACCAGCGTGTGCTGGAGGCTGCAGGTGGCAAACGGGTGGTGTTTCG
CACCCTCGACATCGGGTCCGACAAGGTTCTGCCCTATATGAAGCCCACCGACGAGCCGAACCCAGCCTTGGGATGGCGCG
CAATTCGGGTTGGATTGGACAAACCCGGTGTGATGCGGATGCAGCTACAGGCGCTGATCCGCGCGGCCAACGGCGGGCCT
CTTACGGTGATGTTCCCATTTGTGGCACAATTCGAGGAATACCGCCTCGCGCGCGAGGAGGTGAACAAGACCATCGAACG
CGAGCGCCGTTTGGGACATGTTCTGCCCTCCACGCTCGAGATCGGCGCCATGCTGGAGACCCCATCGCTTGGGTTTGCAC
CGCTCAAGTTCTTTGAGGAAGTCGATTTTCTGTCGATTGGCGGCAATGATCTCAAGCAGTTCTTCTTTGCGGCGGACCGT
GAAAACGAACGGGTGCGACGGCGCTATGACACGCTCAACGTGAGCTTCCTGAGCTTTATCGAGCGGATCGTGGAACGCTG
CGACATCTCCGGCACGCCGCTGAGCTTTTGTGGTGAGGATGCCGGCCGCCCGATCGAAGCCGTGTGTCTTGCAGCCATGG
GAATTCGCACCCTTTCGATGCGTCCGGCCTCGATCGGCCCGGTCAAATCTCTGCTGATGCGCGTGGATCTTGGGGAACTG
CGCAAGATCATCTCTGATGCGCGTCATCGCGGTGATCAAACGGTGCGACCGGCGGTCATGCAGTACCTCCGCGAACTTTG
A

Upstream 100 bases:

>100_bases
ATGCGCATAAGCGTCATCTGCTATTCAGAACGCCGGACCGCGAGCGGGTGAGAAACAGGGCATCCGCAGTGTCGAAGAAG
AAACCCGGGAAAGGGCCCAC

Downstream 100 bases:

>100_bases
TCCATTGGCCGCCATCCTGCCCCGGCCTGTGGACGGCGGTTATGTCTTCTTAAGGAAAATGTGGTTGTACCTTTGCCTCG
AAGAGGCGGGTATTTTGCGG

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MVENTESESRKLLGRLREAMAGDDAGQARLDKITQLIADSMGTEVCSIYLFRDEDTLELCATEGLNRESVHQTRMRVGEG
LVGRVARTGKVINTPDAPSARGFRYMPETGEERFSSFLGIPIQRLGEMMGVLVVQSKESREYSADAVYALEVVAMVIAEM
TELGAFVGEGSAMSPLHQQSVLLKGIAAQEGAAEGNIWLHEPRVVVTNPIADDPHRELERLQEAVEELRVGVDKMLQVSS
NGDKEQAQVLETYRMFANSKGWMRRMEEDIARGLSAEAAVEKEQSLARGRMAQVQDAYLRERLSDLDDLSNRLLRILTGQ
GKDTGAELPENPILVARNIGPAELLEYGRSLKGIILEEGSVGSHAAIVARALAIPLVVRVARITTEGLNGDHVLVDGDNA
VVHLRPDDPVVSAFRDKIAMQAKAQERYSSIREKPAITVDGKRINLMMNAGLMADLPSLENSGAEGVGLFRTELQFLVRN
QMPKRSELKGLYQRVLEAAGGKRVVFRTLDIGSDKVLPYMKPTDEPNPALGWRAIRVGLDKPGVMRMQLQALIRAANGGP
LTVMFPFVAQFEEYRLAREEVNKTIERERRLGHVLPSTLEIGAMLETPSLGFAPLKFFEEVDFLSIGGNDLKQFFFAADR
ENERVRRRYDTLNVSFLSFIERIVERCDISGTPLSFCGEDAGRPIEAVCLAAMGIRTLSMRPASIGPVKSLLMRVDLGEL
RKIISDARHRGDQTVRPAVMQYLREL

Sequences:

>Translated_746_residues
MVENTESESRKLLGRLREAMAGDDAGQARLDKITQLIADSMGTEVCSIYLFRDEDTLELCATEGLNRESVHQTRMRVGEG
LVGRVARTGKVINTPDAPSARGFRYMPETGEERFSSFLGIPIQRLGEMMGVLVVQSKESREYSADAVYALEVVAMVIAEM
TELGAFVGEGSAMSPLHQQSVLLKGIAAQEGAAEGNIWLHEPRVVVTNPIADDPHRELERLQEAVEELRVGVDKMLQVSS
NGDKEQAQVLETYRMFANSKGWMRRMEEDIARGLSAEAAVEKEQSLARGRMAQVQDAYLRERLSDLDDLSNRLLRILTGQ
GKDTGAELPENPILVARNIGPAELLEYGRSLKGIILEEGSVGSHAAIVARALAIPLVVRVARITTEGLNGDHVLVDGDNA
VVHLRPDDPVVSAFRDKIAMQAKAQERYSSIREKPAITVDGKRINLMMNAGLMADLPSLENSGAEGVGLFRTELQFLVRN
QMPKRSELKGLYQRVLEAAGGKRVVFRTLDIGSDKVLPYMKPTDEPNPALGWRAIRVGLDKPGVMRMQLQALIRAANGGP
LTVMFPFVAQFEEYRLAREEVNKTIERERRLGHVLPSTLEIGAMLETPSLGFAPLKFFEEVDFLSIGGNDLKQFFFAADR
ENERVRRRYDTLNVSFLSFIERIVERCDISGTPLSFCGEDAGRPIEAVCLAAMGIRTLSMRPASIGPVKSLLMRVDLGEL
RKIISDARHRGDQTVRPAVMQYLREL
>Mature_746_residues
MVENTESESRKLLGRLREAMAGDDAGQARLDKITQLIADSMGTEVCSIYLFRDEDTLELCATEGLNRESVHQTRMRVGEG
LVGRVARTGKVINTPDAPSARGFRYMPETGEERFSSFLGIPIQRLGEMMGVLVVQSKESREYSADAVYALEVVAMVIAEM
TELGAFVGEGSAMSPLHQQSVLLKGIAAQEGAAEGNIWLHEPRVVVTNPIADDPHRELERLQEAVEELRVGVDKMLQVSS
NGDKEQAQVLETYRMFANSKGWMRRMEEDIARGLSAEAAVEKEQSLARGRMAQVQDAYLRERLSDLDDLSNRLLRILTGQ
GKDTGAELPENPILVARNIGPAELLEYGRSLKGIILEEGSVGSHAAIVARALAIPLVVRVARITTEGLNGDHVLVDGDNA
VVHLRPDDPVVSAFRDKIAMQAKAQERYSSIREKPAITVDGKRINLMMNAGLMADLPSLENSGAEGVGLFRTELQFLVRN
QMPKRSELKGLYQRVLEAAGGKRVVFRTLDIGSDKVLPYMKPTDEPNPALGWRAIRVGLDKPGVMRMQLQALIRAANGGP
LTVMFPFVAQFEEYRLAREEVNKTIERERRLGHVLPSTLEIGAMLETPSLGFAPLKFFEEVDFLSIGGNDLKQFFFAADR
ENERVRRRYDTLNVSFLSFIERIVERCDISGTPLSFCGEDAGRPIEAVCLAAMGIRTLSMRPASIGPVKSLLMRVDLGEL
RKIISDARHRGDQTVRPAVMQYLREL

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=725, Percent_Identity=34.0689655172414, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1788756, Length=442, Percent_Identity=34.3891402714932, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1788726, Length=469, Percent_Identity=34.9680170575693, Blast_Score=253, Evalue=4e-68,
Organism=Escherichia coli, GI48994992, Length=489, Percent_Identity=29.8568507157464, Blast_Score=242, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 82610; Mature: 82610

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVENTESESRKLLGRLREAMAGDDAGQARLDKITQLIADSMGTEVCSIYLFRDEDTLELC
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHH
ATEGLNRESVHQTRMRVGEGLVGRVARTGKVINTPDAPSARGFRYMPETGEERFSSFLGI
HHCCCCHHHHHHHHHHHCCCHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
PIQRLGEMMGVLVVQSKESREYSADAVYALEVVAMVIAEMTELGAFVGEGSAMSPLHQQS
CHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VLLKGIAAQEGAAEGNIWLHEPRVVVTNPIADDPHRELERLQEAVEELRVGVDKMLQVSS
HHHHHHHHCCCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCC
NGDKEQAQVLETYRMFANSKGWMRRMEEDIARGLSAEAAVEKEQSLARGRMAQVQDAYLR
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERLSDLDDLSNRLLRILTGQGKDTGAELPENPILVARNIGPAELLEYGRSLKGIILEEGS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCEEEECCC
VGSHAAIVARALAIPLVVRVARITTEGLNGDHVLVDGDNAVVHLRPDDPVVSAFRDKIAM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHH
QAKAQERYSSIREKPAITVDGKRINLMMNAGLMADLPSLENSGAEGVGLFRTELQFLVRN
HHHHHHHHHHHHHCCCEEECCCEEEEEECCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHH
QMPKRSELKGLYQRVLEAAGGKRVVFRTLDIGSDKVLPYMKPTDEPNPALGWRAIRVGLD
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCC
KPGVMRMQLQALIRAANGGPLTVMFPFVAQFEEYRLAREEVNKTIERERRLGHVLPSTLE
CCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IGAMLETPSLGFAPLKFFEEVDFLSIGGNDLKQFFFAADRENERVRRRYDTLNVSFLSFI
HCEEECCCCCCCHHHHHHHHCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHH
ERIVERCDISGTPLSFCGEDAGRPIEAVCLAAMGIRTLSMRPASIGPVKSLLMRVDLGEL
HHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHH
RKIISDARHRGDQTVRPAVMQYLREL
HHHHHHHHHCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MVENTESESRKLLGRLREAMAGDDAGQARLDKITQLIADSMGTEVCSIYLFRDEDTLELC
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHH
ATEGLNRESVHQTRMRVGEGLVGRVARTGKVINTPDAPSARGFRYMPETGEERFSSFLGI
HHCCCCHHHHHHHHHHHCCCHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
PIQRLGEMMGVLVVQSKESREYSADAVYALEVVAMVIAEMTELGAFVGEGSAMSPLHQQS
CHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VLLKGIAAQEGAAEGNIWLHEPRVVVTNPIADDPHRELERLQEAVEELRVGVDKMLQVSS
HHHHHHHHCCCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCC
NGDKEQAQVLETYRMFANSKGWMRRMEEDIARGLSAEAAVEKEQSLARGRMAQVQDAYLR
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERLSDLDDLSNRLLRILTGQGKDTGAELPENPILVARNIGPAELLEYGRSLKGIILEEGS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCEEEECCC
VGSHAAIVARALAIPLVVRVARITTEGLNGDHVLVDGDNAVVHLRPDDPVVSAFRDKIAM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHH
QAKAQERYSSIREKPAITVDGKRINLMMNAGLMADLPSLENSGAEGVGLFRTELQFLVRN
HHHHHHHHHHHHHCCCEEECCCEEEEEECCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHH
QMPKRSELKGLYQRVLEAAGGKRVVFRTLDIGSDKVLPYMKPTDEPNPALGWRAIRVGLD
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCC
KPGVMRMQLQALIRAANGGPLTVMFPFVAQFEEYRLAREEVNKTIERERRLGHVLPSTLE
CCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IGAMLETPSLGFAPLKFFEEVDFLSIGGNDLKQFFFAADRENERVRRRYDTLNVSFLSFI
HCEEECCCCCCCHHHHHHHHCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHH
ERIVERCDISGTPLSFCGEDAGRPIEAVCLAAMGIRTLSMRPASIGPVKSLLMRVDLGEL
HHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHH
RKIISDARHRGDQTVRPAVMQYLREL
HHHHHHHHHCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]