Click here to switch to the map view.

The map label for this gene is lip2 [H]

Identifier: 99081667

GI number: 99081667

Start: 1920735

End: 1921682

Strand: Reverse

Name: lip2 [H]

Synonym: TM1040_1826

Alternate gene names: 99081667

Gene position: 1921682-1920735 (Counterclockwise)

Preceding gene: 99081668

Following gene: 99081656

Centisome position: 60.03

GC content: 63.19

Gene sequence:

>948_bases
ATGTCAGAGACGGTGAATGTGATGGACTATGAAAAACTGATCGACGAAGAGACGTGGAATTTCATCCGGGAGACGGGGCG
CCACTACCCCGACGACACGGTGGATTTGACGATCGACGATCAGCGCGCGGTCTATAACAAGATGGCCGCTGCCTTTCGCA
AACCCCGCGCCGAGGTGGTGGATGTCACCGAGCGCAGCGTCGGGCGCGTTTTGGTGCGGATCTATGAGGCGGGCGATCCG
ACCCGCACGGTGATGTATTGCCACGGGGGCGGCTTTGTGGTGGGCGATCTCAACAGCCATGATGACGTCTGTTCCGAGAT
TTGCGAGCAGACCGGCTATCGCGTGGTGTCGGTGGATTACCGGCTGGCGCCCGAGCACAAGCATCCCGCCCAGTTCGAGG
ACGCCTGGACGGTGCTGGACTGGATCTGCGAGACCTATCCGGGGGGCGTGGTGCTGGCGGGAGACAGCGCGGGGGCGATG
CTCTCTGCCTGTGTGGCGCATCACGCGCGGGGACGTCACGAGGGCGTGCTGGGGCAGGTGCTGATCTATCCGGGGCTCGG
GGGCGACCACGCCTTGCCCTCTTATCGCGAACATGCGCAGGCGCCGATGCTGACGCTTGAGGATGTGAAATACTACGCCG
ATGTGCGCGCGGATGGCACGCCGGTCGAGAATGATCCGACCTATGCGCCGCTTCAGGACGATGATTTTGCAGGGCTGCCG
CCCACGGTGATGTTCTCTGCCGATTGCGATCCGCTGCGGGACGATTGCCTCGTTTATCGCGATGCGATCCACGCGGCTGG
CGGTCTGGCGGAGTGGATCAATGAGGCGGGGCTGGTGCATGGCTATCTGCGCGCCCGTCACAGCGTCGGCCGGGCGCGCG
ACGGTTTTGAACGTATCGTGCTGGCAATCGAAGGTCTCGGACAGGAGATCTGGCCCTACGACGACTGA

Upstream 100 bases:

>100_bases
ACAGAAAAGCACGCCGCGTCTGGCGTGCTTTTTTCATTTTGCCGTTTTCAACGGGGCTGCGCGGTCCTAGGCTCGAAGGA
GAGAGACGACGCCTGCGAAG

Downstream 100 bases:

>100_bases
GGCGCCTGCAGGAAGGGGTGCGCGTGACGAAGCGCTGTCAGCGCCCTGTTCACCCGGCGAACTGGGCGCACCGCTGCGGA
TAGGCATCAACGCGATACCA

Product: Alpha/beta hydrolase fold-3

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSETVNVMDYEKLIDEETWNFIRETGRHYPDDTVDLTIDDQRAVYNKMAAAFRKPRAEVVDVTERSVGRVLVRIYEAGDP
TRTVMYCHGGGFVVGDLNSHDDVCSEICEQTGYRVVSVDYRLAPEHKHPAQFEDAWTVLDWICETYPGGVVLAGDSAGAM
LSACVAHHARGRHEGVLGQVLIYPGLGGDHALPSYREHAQAPMLTLEDVKYYADVRADGTPVENDPTYAPLQDDDFAGLP
PTVMFSADCDPLRDDCLVYRDAIHAAGGLAEWINEAGLVHGYLRARHSVGRARDGFERIVLAIEGLGQEIWPYDD

Sequences:

>Translated_315_residues
MSETVNVMDYEKLIDEETWNFIRETGRHYPDDTVDLTIDDQRAVYNKMAAAFRKPRAEVVDVTERSVGRVLVRIYEAGDP
TRTVMYCHGGGFVVGDLNSHDDVCSEICEQTGYRVVSVDYRLAPEHKHPAQFEDAWTVLDWICETYPGGVVLAGDSAGAM
LSACVAHHARGRHEGVLGQVLIYPGLGGDHALPSYREHAQAPMLTLEDVKYYADVRADGTPVENDPTYAPLQDDDFAGLP
PTVMFSADCDPLRDDCLVYRDAIHAAGGLAEWINEAGLVHGYLRARHSVGRARDGFERIVLAIEGLGQEIWPYDD
>Mature_314_residues
SETVNVMDYEKLIDEETWNFIRETGRHYPDDTVDLTIDDQRAVYNKMAAAFRKPRAEVVDVTERSVGRVLVRIYEAGDPT
RTVMYCHGGGFVVGDLNSHDDVCSEICEQTGYRVVSVDYRLAPEHKHPAQFEDAWTVLDWICETYPGGVVLAGDSAGAML
SACVAHHARGRHEGVLGQVLIYPGLGGDHALPSYREHAQAPMLTLEDVKYYADVRADGTPVENDPTYAPLQDDDFAGLPP
TVMFSADCDPLRDDCLVYRDAIHAAGGLAEWINEAGLVHGYLRARHSVGRARDGFERIVLAIEGLGQEIWPYDD

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=284, Percent_Identity=28.169014084507, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI157041239, Length=296, Percent_Identity=25.6756756756757, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI206597554, Length=166, Percent_Identity=35.5421686746988, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI61966717, Length=290, Percent_Identity=24.4827586206897, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68051721, Length=177, Percent_Identity=31.638418079096, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI226423947, Length=185, Percent_Identity=30.2702702702703, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1786682, Length=276, Percent_Identity=27.1739130434783, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=138, Percent_Identity=34.7826086956522, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=191, Percent_Identity=31.413612565445, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34971; Mature: 34840

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETVNVMDYEKLIDEETWNFIRETGRHYPDDTVDLTIDDQRAVYNKMAAAFRKPRAEVV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCHHHH
DVTERSVGRVLVRIYEAGDPTRTVMYCHGGGFVVGDLNSHDDVCSEICEQTGYRVVSVDY
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHCCCEEEEEEE
RLAPEHKHPAQFEDAWTVLDWICETYPGGVVLAGDSAGAMLSACVAHHARGRHEGVLGQV
EECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEEE
LIYPGLGGDHALPSYREHAQAPMLTLEDVKYYADVRADGTPVENDPTYAPLQDDDFAGLP
EEECCCCCCCCCCCHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PTVMFSADCDPLRDDCLVYRDAIHAAGGLAEWINEAGLVHGYLRARHSVGRARDGFERIV
CCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAIEGLGQEIWPYDD
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
SETVNVMDYEKLIDEETWNFIRETGRHYPDDTVDLTIDDQRAVYNKMAAAFRKPRAEVV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCHHHH
DVTERSVGRVLVRIYEAGDPTRTVMYCHGGGFVVGDLNSHDDVCSEICEQTGYRVVSVDY
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHCCCEEEEEEE
RLAPEHKHPAQFEDAWTVLDWICETYPGGVVLAGDSAGAMLSACVAHHARGRHEGVLGQV
EECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEEE
LIYPGLGGDHALPSYREHAQAPMLTLEDVKYYADVRADGTPVENDPTYAPLQDDDFAGLP
EEECCCCCCCCCCCHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PTVMFSADCDPLRDDCLVYRDAIHAAGGLAEWINEAGLVHGYLRARHSVGRARDGFERIV
CCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAIEGLGQEIWPYDD
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]