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The map label for this gene is pcm

Identifier: 99081633

GI number: 99081633

Start: 1885491

End: 1886138

Strand: Direct

Name: pcm

Synonym: TM1040_1792

Alternate gene names: 99081633

Gene position: 1885491-1886138 (Clockwise)

Preceding gene: 99081632

Following gene: 99081634

Centisome position: 58.9

GC content: 62.96

Gene sequence:

>648_bases
ATGAGCGGGCCTGACGCGCAAACCAAGATGCAGTTTCTCTTTGCCCTGCGCTCGCGCGGGGTCACCGAAGCTGCCGTTCT
GGAAGCCATGGAGGCCGTGGATCGCGGCCTGTTCCTGCGCGGCATTTTTTCGGAACGCGCCTATGAGGATGTGCCGCTGC
CGATTGCCTGTGGTCAGACGATTTCGCAGCCCTCCGTTGTGGGCCTGATGACTCAGGCGCTGCAGGTCAATCCCCGCGAC
ACCGTGCTCGAGGTGGGGACCGGCTCGGGCTATCAGGCGGCGATCCTCGCCAAGCTGGCGCGCCGAGTCTATACTGTCGA
CCGCCACGGACGCCTTGTGCGCGAGGCTCGGCAGGTGTTCCAGCAAATGAGCCTGTCCAATATCACATCCCTGGTGGGCG
ATGGCAGCCATGGCATGCCGGATCAGGCGCCGTTTGATCGAATCATCGTCACCGCCGCTGCCGAAGATCCCCCCGGCCCG
CTCTTGGCGCAGTTGAAGGTCGGCGGCATCATGGTGGTCCCGGTGGGCCAATCGGATGCGGTCCAGACCCTGATCCGCGT
CCGAAAGACCGAAAATGGGCTGGAATATGACGAATTGCGCCCCGTGCGCTTTGTTCCTCTGCTGGAAGGGCTTGGAAAAG
ACACCTGA

Upstream 100 bases:

>100_bases
AAACGGACGCGGCGGCAAACCTCGACGGGTTCATCTCTGTCACTCCCATGCGCGCGGATCTCACTGCGCATGACGCTGTG
GAGGCCTTGAAGGCGATCGA

Downstream 100 bases:

>100_bases
GCTGCCTTATTTCGGAGAGAGCCCCGTCGCGCGGCGCAGAGACCCGGTTCAGGCCCGGGCCGCCAGATACGCCGCCGCCG
TCAAAGTCTCCGCCAGAACG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSGPDAQTKMQFLFALRSRGVTEAAVLEAMEAVDRGLFLRGIFSERAYEDVPLPIACGQTISQPSVVGLMTQALQVNPRD
TVLEVGTGSGYQAAILAKLARRVYTVDRHGRLVREARQVFQQMSLSNITSLVGDGSHGMPDQAPFDRIIVTAAAEDPPGP
LLAQLKVGGIMVVPVGQSDAVQTLIRVRKTENGLEYDELRPVRFVPLLEGLGKDT

Sequences:

>Translated_215_residues
MSGPDAQTKMQFLFALRSRGVTEAAVLEAMEAVDRGLFLRGIFSERAYEDVPLPIACGQTISQPSVVGLMTQALQVNPRD
TVLEVGTGSGYQAAILAKLARRVYTVDRHGRLVREARQVFQQMSLSNITSLVGDGSHGMPDQAPFDRIIVTAAAEDPPGP
LLAQLKVGGIMVVPVGQSDAVQTLIRVRKTENGLEYDELRPVRFVPLLEGLGKDT
>Mature_214_residues
SGPDAQTKMQFLFALRSRGVTEAAVLEAMEAVDRGLFLRGIFSERAYEDVPLPIACGQTISQPSVVGLMTQALQVNPRDT
VLEVGTGSGYQAAILAKLARRVYTVDRHGRLVREARQVFQQMSLSNITSLVGDGSHGMPDQAPFDRIIVTAAAEDPPGPL
LAQLKVGGIMVVPVGQSDAVQTLIRVRKTENGLEYDELRPVRFVPLLEGLGKDT

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=211, Percent_Identity=30.3317535545024, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1789100, Length=199, Percent_Identity=44.7236180904523, Blast_Score=162, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71983477, Length=212, Percent_Identity=30.6603773584906, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=210, Percent_Identity=29.5238095238095, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17981723, Length=211, Percent_Identity=32.2274881516588, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_SILST (Q1GFP1)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613787.1
- ProteinModelPortal:   Q1GFP1
- SMR:   Q1GFP1
- STRING:   Q1GFP1
- GeneID:   4076821
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1792
- NMPDR:   fig|292414.1.peg.233
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- PhylomeDB:   Q1GFP1
- ProtClustDB:   CLSK933941
- BioCyc:   SSP292414:TM1040_1792-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23257; Mature: 23126

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPDAQTKMQFLFALRSRGVTEAAVLEAMEAVDRGLFLRGIFSERAYEDVPLPIACGQT
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
ISQPSVVGLMTQALQVNPRDTVLEVGTGSGYQAAILAKLARRVYTVDRHGRLVREARQVF
CCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQMSLSNITSLVGDGSHGMPDQAPFDRIIVTAAAEDPPGPLLAQLKVGGIMVVPVGQSDA
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCEEEEECCCCHH
VQTLIRVRKTENGLEYDELRPVRFVPLLEGLGKDT
HHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SGPDAQTKMQFLFALRSRGVTEAAVLEAMEAVDRGLFLRGIFSERAYEDVPLPIACGQT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
ISQPSVVGLMTQALQVNPRDTVLEVGTGSGYQAAILAKLARRVYTVDRHGRLVREARQVF
CCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQMSLSNITSLVGDGSHGMPDQAPFDRIIVTAAAEDPPGPLLAQLKVGGIMVVPVGQSDA
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCEEEEECCCCHH
VQTLIRVRKTENGLEYDELRPVRFVPLLEGLGKDT
HHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA