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The map label for this gene is purF [H]

Identifier: 99081628

GI number: 99081628

Start: 1880937

End: 1882457

Strand: Direct

Name: purF [H]

Synonym: TM1040_1787

Alternate gene names: 99081628

Gene position: 1880937-1882457 (Clockwise)

Preceding gene: 99081627

Following gene: 99081629

Centisome position: 58.76

GC content: 59.43

Gene sequence:

>1521_bases
ATGTGCGGTATCTTGGGCATCGCAAGCAGAAACGAACAAGTGTTTGCGGAACTCTATGACGGGCTGCTGATGCTGCAGCA
TCGCGGTCAGGATGCGTCAGGCATCGTCACCTACGGCGATGGGTTCTTTCGCGAACGCAAGGCCAACGGTCTGGTGAAGG
ACGTCTTTGGTGCCAAAGACGCCGAGACCCTGCAAGGCCGTGTCGGGATGGGCCATGTGCGCTACCCCACCGCAGGTTCG
CTCTCGGCCGCCGAGGCGCAGCCGTTTTTTGTGAACGCACCCTATGGGATCTACCTGGTCCACAACGGCAATATCACCAA
CACCGAAGAGCAGCGCGCAAAGGTCACCGGCAAATACAGCCGCCACCTGCGCACCACCTCGGATTCCGAGATCCTGCTCA
ACGTGCTGGCGGATAAGGTGGCTGACGCGATCAAGGTCAATGGCAACGCGGATCCGATCCGCAACATCTTTGCCGGTGTG
AAAATGACCATGGAGCGCGTCCAGGGCGCCTATTCCGTGATCTGCCTCATCGCCGGCGTCGGCATGCTGGCCTTCCGCGA
CCCCCATGGCATCCGCCCGCTCTCCGTGGCCCAACGCGAAGCCGAGGGTGAGGGCAATGATTATGCCTTTGCCTCCGAGG
ATGTGGCTTTTGGCATCAACGGCTTTGAAAAACTGCGGGACCTCAAACCCGGCGAGGCCATCCTCGTCGATCTTGACGGC
AACAAGCACGAGTTCCAGGCGGTCGAAGGCAAGCTCACCCCTTGTATCTTCGAATATGTCTACCTCGCCCGCCCCGACAG
CCTCTTGGACGGGATCTCGGTATATAAAACCCAGATCCGCATGGGGCAGACACTCGCCAAGCAGATTGAGGCATCCGGCC
TCGACATCGACGCCATCATCCCGGTGCCAGACAGCGCCCGCCCCGTGGCACTTGAGGTTTCCAACATCACCGGCATTCGC
TACCGCGAGGCGCTGGTGAAAAACCGCTATGTGGGCCGAACATTCATCATGCCCGGGCAAGAAGAACGCCAGAAATCCGT
GCGCCGCAAATTGAACGCGATCCCGCGCGAGATGCAGGACCGCAACATCCTGCTCATTGATGACTCCATCGTGCGGGGCA
ATACGATCAAGAAGATCGTGCAGATGTGTCGCGAGGCCGGTGCCAAGAAGGTCTATATCGCCTCCGCTTCGCCGCCGGTG
AAATACCCCAATGTCTATGGCATCGACATGCCCACCAAACACGAACTGGTGGCCAACGGGCGCTCGATCGAGGAGATTCG
CGAGGAGTTGGGGGCGGATGAGCTGTTCTACCAGAACCTCGAGGACCTGATCTGGGCCGCCAAAGGTGGAAACCCGGACA
TCGAAGCCTTTGATTGTTCGTGTTTTGACGGCGAGTATGTCACCGGCTCGGTCAGCGAGGACTACCTCAACACGCTCGAA
ACCAGCACCCGCGTGACCAAGAAAATCAAGGACATGCCTGATGCAGGCGCATCCTGGAACGGCGCAACGCTGGCCACCTG
A

Upstream 100 bases:

>100_bases
TCCCCTGTTGCGGCCATTGTCGTTGGCCTCTGCGCCCCGACATCTCACGCCGCGGCCTTCGGGCTGTAAGCCACTCGAAC
GATTAATTTGGAGCCTCGCC

Downstream 100 bases:

>100_bases
GCCCTGAGCCCCCCATGCGCGGATCTGCGCCCGTCACGCAGCCGTCACGCGCCTGTGCGGGCGCAGTGATTGCAAGTCTG
CGCCAACGGGCGTAAGGGGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MCGILGIASRNEQVFAELYDGLLMLQHRGQDASGIVTYGDGFFRERKANGLVKDVFGAKDAETLQGRVGMGHVRYPTAGS
LSAAEAQPFFVNAPYGIYLVHNGNITNTEEQRAKVTGKYSRHLRTTSDSEILLNVLADKVADAIKVNGNADPIRNIFAGV
KMTMERVQGAYSVICLIAGVGMLAFRDPHGIRPLSVAQREAEGEGNDYAFASEDVAFGINGFEKLRDLKPGEAILVDLDG
NKHEFQAVEGKLTPCIFEYVYLARPDSLLDGISVYKTQIRMGQTLAKQIEASGLDIDAIIPVPDSARPVALEVSNITGIR
YREALVKNRYVGRTFIMPGQEERQKSVRRKLNAIPREMQDRNILLIDDSIVRGNTIKKIVQMCREAGAKKVYIASASPPV
KYPNVYGIDMPTKHELVANGRSIEEIREELGADELFYQNLEDLIWAAKGGNPDIEAFDCSCFDGEYVTGSVSEDYLNTLE
TSTRVTKKIKDMPDAGASWNGATLAT

Sequences:

>Translated_506_residues
MCGILGIASRNEQVFAELYDGLLMLQHRGQDASGIVTYGDGFFRERKANGLVKDVFGAKDAETLQGRVGMGHVRYPTAGS
LSAAEAQPFFVNAPYGIYLVHNGNITNTEEQRAKVTGKYSRHLRTTSDSEILLNVLADKVADAIKVNGNADPIRNIFAGV
KMTMERVQGAYSVICLIAGVGMLAFRDPHGIRPLSVAQREAEGEGNDYAFASEDVAFGINGFEKLRDLKPGEAILVDLDG
NKHEFQAVEGKLTPCIFEYVYLARPDSLLDGISVYKTQIRMGQTLAKQIEASGLDIDAIIPVPDSARPVALEVSNITGIR
YREALVKNRYVGRTFIMPGQEERQKSVRRKLNAIPREMQDRNILLIDDSIVRGNTIKKIVQMCREAGAKKVYIASASPPV
KYPNVYGIDMPTKHELVANGRSIEEIREELGADELFYQNLEDLIWAAKGGNPDIEAFDCSCFDGEYVTGSVSEDYLNTLE
TSTRVTKKIKDMPDAGASWNGATLAT
>Mature_506_residues
MCGILGIASRNEQVFAELYDGLLMLQHRGQDASGIVTYGDGFFRERKANGLVKDVFGAKDAETLQGRVGMGHVRYPTAGS
LSAAEAQPFFVNAPYGIYLVHNGNITNTEEQRAKVTGKYSRHLRTTSDSEILLNVLADKVADAIKVNGNADPIRNIFAGV
KMTMERVQGAYSVICLIAGVGMLAFRDPHGIRPLSVAQREAEGEGNDYAFASEDVAFGINGFEKLRDLKPGEAILVDLDG
NKHEFQAVEGKLTPCIFEYVYLARPDSLLDGISVYKTQIRMGQTLAKQIEASGLDIDAIIPVPDSARPVALEVSNITGIR
YREALVKNRYVGRTFIMPGQEERQKSVRRKLNAIPREMQDRNILLIDDSIVRGNTIKKIVQMCREAGAKKVYIASASPPV
KYPNVYGIDMPTKHELVANGRSIEEIREELGADELFYQNLEDLIWAAKGGNPDIEAFDCSCFDGEYVTGSVSEDYLNTLE
TSTRVTKKIKDMPDAGASWNGATLAT

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=479, Percent_Identity=36.7432150313152, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1788651, Length=485, Percent_Identity=53.8144329896907, Blast_Score=522, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=35.1239669421488, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6323958, Length=489, Percent_Identity=47.6482617586912, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24659598, Length=470, Percent_Identity=35.1063829787234, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24659604, Length=470, Percent_Identity=35.1063829787234, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI28573187, Length=473, Percent_Identity=34.0380549682875, Blast_Score=252, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55602; Mature: 55602

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGILGIASRNEQVFAELYDGLLMLQHRGQDASGIVTYGDGFFRERKANGLVKDVFGAKD
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHCCHHHHHHCCCC
AETLQGRVGMGHVRYPTAGSLSAAEAQPFFVNAPYGIYLVHNGNITNTEEQRAKVTGKYS
HHHHHCCCCCCEEECCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHH
RHLRTTSDSEILLNVLADKVADAIKVNGNADPIRNIFAGVKMTMERVQGAYSVICLIAGV
HHCCCCCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GMLAFRDPHGIRPLSVAQREAEGEGNDYAFASEDVAFGINGFEKLRDLKPGEAILVDLDG
CCEEECCCCCCCCHHHHHHHCCCCCCCCEEECCCEEECCCHHHHHHCCCCCCEEEEEECC
NKHEFQAVEGKLTPCIFEYVYLARPDSLLDGISVYKTQIRMGQTLAKQIEASGLDIDAII
CCCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PVPDSARPVALEVSNITGIRYREALVKNRYVGRTFIMPGQEERQKSVRRKLNAIPREMQD
ECCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCHHHCC
RNILLIDDSIVRGNTIKKIVQMCREAGAKKVYIASASPPVKYPNVYGIDMPTKHELVANG
CEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHCC
RSIEEIREELGADELFYQNLEDLIWAAKGGNPDIEAFDCSCFDGEYVTGSVSEDYLNTLE
CCHHHHHHHHCCHHHHHHCHHHHHEECCCCCCCCCEEECEECCCCEEECCCCHHHHHHHH
TSTRVTKKIKDMPDAGASWNGATLAT
HHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGILGIASRNEQVFAELYDGLLMLQHRGQDASGIVTYGDGFFRERKANGLVKDVFGAKD
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHCCHHHHHHCCCC
AETLQGRVGMGHVRYPTAGSLSAAEAQPFFVNAPYGIYLVHNGNITNTEEQRAKVTGKYS
HHHHHCCCCCCEEECCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHH
RHLRTTSDSEILLNVLADKVADAIKVNGNADPIRNIFAGVKMTMERVQGAYSVICLIAGV
HHCCCCCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GMLAFRDPHGIRPLSVAQREAEGEGNDYAFASEDVAFGINGFEKLRDLKPGEAILVDLDG
CCEEECCCCCCCCHHHHHHHCCCCCCCCEEECCCEEECCCHHHHHHCCCCCCEEEEEECC
NKHEFQAVEGKLTPCIFEYVYLARPDSLLDGISVYKTQIRMGQTLAKQIEASGLDIDAII
CCCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PVPDSARPVALEVSNITGIRYREALVKNRYVGRTFIMPGQEERQKSVRRKLNAIPREMQD
ECCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCHHHCC
RNILLIDDSIVRGNTIKKIVQMCREAGAKKVYIASASPPVKYPNVYGIDMPTKHELVANG
CEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHCC
RSIEEIREELGADELFYQNLEDLIWAAKGGNPDIEAFDCSCFDGEYVTGSVSEDYLNTLE
CCHHHHHHHHCCHHHHHHCHHHHHEECCCCCCCCCEEECEECCCCEEECCCCHHHHHHHH
TSTRVTKKIKDMPDAGASWNGATLAT
HHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]