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The map label for this gene is pyrC

Identifier: 99081617

GI number: 99081617

Start: 1869523

End: 1870563

Strand: Direct

Name: pyrC

Synonym: TM1040_1776

Alternate gene names: 99081617

Gene position: 1869523-1870563 (Clockwise)

Preceding gene: 99081616

Following gene: 99081618

Centisome position: 58.41

GC content: 62.34

Gene sequence:

>1041_bases
ATGACCCAGAGCCTGACGATCACCCGCCCCGACGACTGGCATCTGCATCTGCGCGACGGCGACATGCTGCGCGCGGTGCT
GCCGGAAACAGCCCGCCATTTTGGCCGCGCCATCATCATGCCCAATCTCGTGCCCCCGGTTGTCACCGGCGCCGAGGCCA
GCGCCTATCGCGACCGCATTCTTGCCGCACTCCCTGAGGGCATGACGTTCGAGCCCCTGATGACGCTCTATCTCACCGAG
GACACGGACCCTGCAGATGTCGCAGCCGCCCATGCCTCGGGTCTGGTCAAAGCCGTCAAGCTCTACCCCGCTGGCGCCAC
CACCAACTCCTCGTCCGGTGTGCGCGATTTCGACAAGGTCCGCCCCGTGCTTGAGAAAATGGCCGAAATCGGCCTGCCGC
TCTGCACCCATGGCGAGGTCACCGACCACGACATCGACATCTTTGACCGCGAGGCCGTCTTTATCGATCGCGTGCTGGAC
CCGATCCGCCAATCCACACCGGGCCTGCGTGTGGTGATGGAGCATATCACCACCAAGGACGCGGCGGATTATGTGCGATC
GCAGGACAAGGATCTTGGCGCGACAATCACCACGCACCACCTGATCATCAATCGCAACCACATCCTCGTGGGCGGGATCA
AGCCACACTATTACTGCCTGCCTGTCGCCAAGCGCGAAGAGCACCGCCTCGCCCTGCGCCAAGCTGCGACCTCCGGTGAT
GCGCGGTTCTTCCTTGGTACCGACTCAGCGCCCCACACCGATGCCAACAAGCTCCAGACCTGTGGCTGCGCGGGTTGTTT
CACGGCGACCAACACCATGGCTCTGCTGGCCCATGTATTTGAGGAAGAAGGCGCGCTCGACAAGCTCGAAGGGTTTGCCT
CCAAGAACGGCCCCGCCTTTTATCGCTTACCCGAAAACGATGGTCAGATCACACTGGTGAAACAGGACGCGCCCGTCGCT
TTCCCGGAACAGATCGACACGCCGGACGGCCCCGTGACCGTCTTTGATCCAAGCTTTGCGGTGCATTGGACCGTCACCTG
A

Upstream 100 bases:

>100_bases
CATGCCGCCGGGTCCCGCTCTGCCCCCTGATCCGCAACACGGCGACGACGGCCTCTAAGCTGCCTTCCCAAGTTCATGGG
GCTGTGCTAACGCCTGCGCC

Downstream 100 bases:

>100_bases
CTTCATCTTCTCGATAAATATCCCCGCCGGAGGCTCCCGCCCGCGCCTCTGGCACTCTGCTCTCGAAGGATACGCCATGA
TCCCCTCCTCCTACCCCTCC

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTQSLTITRPDDWHLHLRDGDMLRAVLPETARHFGRAIIMPNLVPPVVTGAEASAYRDRILAALPEGMTFEPLMTLYLTE
DTDPADVAAAHASGLVKAVKLYPAGATTNSSSGVRDFDKVRPVLEKMAEIGLPLCTHGEVTDHDIDIFDREAVFIDRVLD
PIRQSTPGLRVVMEHITTKDAADYVRSQDKDLGATITTHHLIINRNHILVGGIKPHYYCLPVAKREEHRLALRQAATSGD
ARFFLGTDSAPHTDANKLQTCGCAGCFTATNTMALLAHVFEEEGALDKLEGFASKNGPAFYRLPENDGQITLVKQDAPVA
FPEQIDTPDGPVTVFDPSFAVHWTVT

Sequences:

>Translated_346_residues
MTQSLTITRPDDWHLHLRDGDMLRAVLPETARHFGRAIIMPNLVPPVVTGAEASAYRDRILAALPEGMTFEPLMTLYLTE
DTDPADVAAAHASGLVKAVKLYPAGATTNSSSGVRDFDKVRPVLEKMAEIGLPLCTHGEVTDHDIDIFDREAVFIDRVLD
PIRQSTPGLRVVMEHITTKDAADYVRSQDKDLGATITTHHLIINRNHILVGGIKPHYYCLPVAKREEHRLALRQAATSGD
ARFFLGTDSAPHTDANKLQTCGCAGCFTATNTMALLAHVFEEEGALDKLEGFASKNGPAFYRLPENDGQITLVKQDAPVA
FPEQIDTPDGPVTVFDPSFAVHWTVT
>Mature_345_residues
TQSLTITRPDDWHLHLRDGDMLRAVLPETARHFGRAIIMPNLVPPVVTGAEASAYRDRILAALPEGMTFEPLMTLYLTED
TDPADVAAAHASGLVKAVKLYPAGATTNSSSGVRDFDKVRPVLEKMAEIGLPLCTHGEVTDHDIDIFDREAVFIDRVLDP
IRQSTPGLRVVMEHITTKDAADYVRSQDKDLGATITTHHLIINRNHILVGGIKPHYYCLPVAKREEHRLALRQAATSGDA
RFFLGTDSAPHTDANKLQTCGCAGCFTATNTMALLAHVFEEEGALDKLEGFASKNGPAFYRLPENDGQITLVKQDAPVAF
PEQIDTPDGPVTVFDPSFAVHWTVT

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=344, Percent_Identity=56.1046511627907, Blast_Score=387, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323452, Length=369, Percent_Identity=33.6043360433604, Blast_Score=173, Evalue=4e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_SILST (Q1GFQ7)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613771.1
- ProteinModelPortal:   Q1GFQ7
- SMR:   Q1GFQ7
- STRING:   Q1GFQ7
- GeneID:   4076805
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1776
- NMPDR:   fig|292414.1.peg.249
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- PhylomeDB:   Q1GFQ7
- ProtClustDB:   PRK05451
- BioCyc:   SSP292414:TM1040_1776-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 37869; Mature: 37738

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSLTITRPDDWHLHLRDGDMLRAVLPETARHFGRAIIMPNLVPPVVTGAEASAYRDRI
CCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHH
LAALPEGMTFEPLMTLYLTEDTDPADVAAAHASGLVKAVKLYPAGATTNSSSGVRDFDKV
HHHCCCCCEECCEEEEEEECCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHH
RPVLEKMAEIGLPLCTHGEVTDHDIDIFDREAVFIDRVLDPIRQSTPGLRVVMEHITTKD
HHHHHHHHHCCCCEECCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCHH
AADYVRSQDKDLGATITTHHLIINRNHILVGGIKPHYYCLPVAKREEHRLALRQAATSGD
HHHHHHCCCCCCCCEEEEEEEEEECCEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCC
ARFFLGTDSAPHTDANKLQTCGCAGCFTATNTMALLAHVFEEEGALDKLEGFASKNGPAF
EEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEE
YRLPENDGQITLVKQDAPVAFPEQIDTPDGPVTVFDPSFAVHWTVT
EECCCCCCEEEEEECCCCCCCCHHCCCCCCCEEEECCCEEEEEEEC
>Mature Secondary Structure 
TQSLTITRPDDWHLHLRDGDMLRAVLPETARHFGRAIIMPNLVPPVVTGAEASAYRDRI
CCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHH
LAALPEGMTFEPLMTLYLTEDTDPADVAAAHASGLVKAVKLYPAGATTNSSSGVRDFDKV
HHHCCCCCEECCEEEEEEECCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHH
RPVLEKMAEIGLPLCTHGEVTDHDIDIFDREAVFIDRVLDPIRQSTPGLRVVMEHITTKD
HHHHHHHHHCCCCEECCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCHH
AADYVRSQDKDLGATITTHHLIINRNHILVGGIKPHYYCLPVAKREEHRLALRQAATSGD
HHHHHHCCCCCCCCEEEEEEEEEECCEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCC
ARFFLGTDSAPHTDANKLQTCGCAGCFTATNTMALLAHVFEEEGALDKLEGFASKNGPAF
EEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEE
YRLPENDGQITLVKQDAPVAFPEQIDTPDGPVTVFDPSFAVHWTVT
EECCCCCCEEEEEECCCCCCCCHHCCCCCCCEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA