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The map label for this gene is pyrE

Identifier: 99081618

GI number: 99081618

Start: 1870640

End: 1871320

Strand: Direct

Name: pyrE

Synonym: TM1040_1777

Alternate gene names: 99081618

Gene position: 1870640-1871320 (Clockwise)

Preceding gene: 99081617

Following gene: 99081619

Centisome position: 58.44

GC content: 60.5

Gene sequence:

>681_bases
ATGATCCCCTCCTCCTACCCCTCCGCCGAAGAAATTGCCCGCCTCTCTGCCCGCATGCTCTTGGAAATCGGCGCGGTGAA
CTTCAACACCGATGAACCCTACACGCTGGCTTCGGGCCTGCCGTCGCCGAGCTACATCGACTGCCGCAAGCTGATTTCCT
TCCCGCGCATCCGCTCCACCCTGATGGATTTCATGGCTGTCACGGTGATGCGCAACGCAGGCTTTGAGGCCTTCGACAAT
ATCGCGGGCGGCGAAACCGCAGGCATCCCCTTTGCCGCGCTGGTGGCCGAACGCCTCGCCCTGCCAATGACTTATGTGCG
TAAAAAACCCAAAGGCTATGGCCGCAATGCCCGTATTGAGGGCGCCATGTCCGAAGGCGAGCGCGTCCTGCTGGTCGAAG
ATCTGACAACCGATGGCGGTTCCAAGCTTTCTTTCGTGGACGCGATCCGCGAAACCGGTGCCACCTGCGGGCACACGGCG
GTGATCTTCTATTACGACATCTTCCCCGAGACCACAAAAACGCTCGGCGATCACGGCGTACAGCTGCATTATCTCTGCAC
CTGGTGGGATGTGCTGGCCGAGGCGCGCGCGCAGGCTGCCTTCAGCACCGAAACCCTTGACGAGGTAGAGAAATTCCTGC
GTGATCCGCGCGCCTGGCAAGAGGCCAACAAGAAGACCTGA

Upstream 100 bases:

>100_bases
GCGGTGCATTGGACCGTCACCTGACTTCATCTTCTCGATAAATATCCCCGCCGGAGGCTCCCGCCCGCGCCTCTGGCACT
CTGCTCTCGAAGGATACGCC

Downstream 100 bases:

>100_bases
GGAAAACCTGTCCTTCAGGGCATCTGTCATCTGTGGATAAGGCCGCGCCGAATCGGAGCGGCCTTTCCCATATCTCGCGG
CTGACGCGCGTTTGACACAC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MIPSSYPSAEEIARLSARMLLEIGAVNFNTDEPYTLASGLPSPSYIDCRKLISFPRIRSTLMDFMAVTVMRNAGFEAFDN
IAGGETAGIPFAALVAERLALPMTYVRKKPKGYGRNARIEGAMSEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTA
VIFYYDIFPETTKTLGDHGVQLHYLCTWWDVLAEARAQAAFSTETLDEVEKFLRDPRAWQEANKKT

Sequences:

>Translated_226_residues
MIPSSYPSAEEIARLSARMLLEIGAVNFNTDEPYTLASGLPSPSYIDCRKLISFPRIRSTLMDFMAVTVMRNAGFEAFDN
IAGGETAGIPFAALVAERLALPMTYVRKKPKGYGRNARIEGAMSEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTA
VIFYYDIFPETTKTLGDHGVQLHYLCTWWDVLAEARAQAAFSTETLDEVEKFLRDPRAWQEANKKT
>Mature_226_residues
MIPSSYPSAEEIARLSARMLLEIGAVNFNTDEPYTLASGLPSPSYIDCRKLISFPRIRSTLMDFMAVTVMRNAGFEAFDN
IAGGETAGIPFAALVAERLALPMTYVRKKPKGYGRNARIEGAMSEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTA
VIFYYDIFPETTKTLGDHGVQLHYLCTWWDVLAEARAQAAFSTETLDEVEKFLRDPRAWQEANKKT

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=182, Percent_Identity=32.967032967033, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17508631, Length=123, Percent_Identity=39.8373983739837, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=206, Percent_Identity=27.6699029126214, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17933654, Length=200, Percent_Identity=27, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_SILST (Q1GFQ6)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613772.1
- ProteinModelPortal:   Q1GFQ6
- SMR:   Q1GFQ6
- STRING:   Q1GFQ6
- GeneID:   4076806
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1777
- NMPDR:   fig|292414.1.peg.248
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   LPMTYVR
- PhylomeDB:   Q1GFQ6
- ProtClustDB:   PRK00455
- BioCyc:   SSP292414:TM1040_1777-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 25003; Mature: 25003

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 107-107 BINDING 108-108 BINDING 111-111 BINDING 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPSSYPSAEEIARLSARMLLEIGAVNFNTDEPYTLASGLPSPSYIDCRKLISFPRIRST
CCCCCCCCHHHHHHHHHHHHHHHCCEECCCCCCEEHHCCCCCCCHHHHHHHHCCHHHHHH
LMDFMAVTVMRNAGFEAFDNIAGGETAGIPFAALVAERLALPMTYVRKKPKGYGRNARIE
HHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEC
GAMSEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTAVIFYYDIFPETTKTLGDHGV
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHCCCC
QLHYLCTWWDVLAEARAQAAFSTETLDEVEKFLRDPRAWQEANKKT
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure
MIPSSYPSAEEIARLSARMLLEIGAVNFNTDEPYTLASGLPSPSYIDCRKLISFPRIRST
CCCCCCCCHHHHHHHHHHHHHHHCCEECCCCCCEEHHCCCCCCCHHHHHHHHCCHHHHHH
LMDFMAVTVMRNAGFEAFDNIAGGETAGIPFAALVAERLALPMTYVRKKPKGYGRNARIE
HHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEC
GAMSEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTAVIFYYDIFPETTKTLGDHGV
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHCCCC
QLHYLCTWWDVLAEARAQAAFSTETLDEVEKFLRDPRAWQEANKKT
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA