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The map label for this gene is ycgF [H]

Identifier: 99081588

GI number: 99081588

Start: 1840384

End: 1841259

Strand: Direct

Name: ycgF [H]

Synonym: TM1040_1747

Alternate gene names: 99081588

Gene position: 1840384-1841259 (Clockwise)

Preceding gene: 99081581

Following gene: 99081590

Centisome position: 57.5

GC content: 54.68

Gene sequence:

>876_bases
ATGCTGCGGGACAAGCGAATAGCGCTCTGGACAAGGGTTTCTTCACCTTCTTGCCATAAAGAAGAGCTTAGAGATGGAAG
GGAGATGACCTTGACGGATACAGAAAAGTGCAAAGCATGTCGCAACGGCAAAGGCTTCGAAGTCCCCATTTCCATGGCTT
TTCATCCGATTGTCTCCGTCGGCTCCGGCAAAGTATTTGCCTATGAGGCCCTGGTGCGCGGACAGGATGGGTCTGGTGCT
GCGCAGGTCTTTGAGCAGGTCAATGACGACAACCTCTATGCCTTCGACCAGAAATGCCGCAAGACCGCGATCGAACACGC
CAGTCGCCTTGATCTCGGCGACACCGGGTCGCTCTTGTCGATCAACTTCTTGCCCAATGCGGTTTATGATCCCAAAGCCT
GCATTCGCCTGACCTTGGAGACCGCAAAAAAGACCGGGTTTCCCGTGTCCCAGATCATGTTTGAGTTCACCGAGGTCGAA
CGGATCTCTGCGCCGCATCTCCTCGACATCCTCAGCACCTACCGTCGCCTTGGTTTTTTGACCTCGATCGACGACTTCGG
CGCTGGATACGCCGGGTTAGATCTATTGTCCCGGTTTCAGCCGGATGTCGTGAAACTCGACGCCGAACTCACCCGAAATA
TTGATCAGTCAAGCGTCAAACAGACCATCGTGCGGCACGTCATCTCCATGCTGGAGCAGCTTGGCATCATCATCGTTGCA
GAAGGTATTGAAACGCAGGGGGAATTCTCCTGCCTCTATGACCTAGGAGTTGACCTGATGCAGGGCTGGCTGTTTGCAAA
ACCAGGGTTTGAGACCCTTCCGGATGTCCGCTGGCCCGACGCGCTTCGGAGTGTCGAGGAAAGCAAAACCGCCTGA

Upstream 100 bases:

>100_bases
GGTTGAATATGCAACCTTTTGCAGCGCGCCCGCGCGTCTTTTTCTGCAGGTTTCGCGCCAGTGTGAGAGGCCCGTTCCAG
ACAGAACCGCCCGGCTCAGA

Downstream 100 bases:

>100_bases
TGCCCTCGCAGGCGCGTCGTCCGCATCCGGCGTGCGAGCCGAACATGGGTATATAGCCGGCTCGAGGGGCCCCCGAAGTG
CGGAACTCTGCGCCAAACGA

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLRDKRIALWTRVSSPSCHKEELRDGREMTLTDTEKCKACRNGKGFEVPISMAFHPIVSVGSGKVFAYEALVRGQDGSGA
AQVFEQVNDDNLYAFDQKCRKTAIEHASRLDLGDTGSLLSINFLPNAVYDPKACIRLTLETAKKTGFPVSQIMFEFTEVE
RISAPHLLDILSTYRRLGFLTSIDDFGAGYAGLDLLSRFQPDVVKLDAELTRNIDQSSVKQTIVRHVISMLEQLGIIIVA
EGIETQGEFSCLYDLGVDLMQGWLFAKPGFETLPDVRWPDALRSVEESKTA

Sequences:

>Translated_291_residues
MLRDKRIALWTRVSSPSCHKEELRDGREMTLTDTEKCKACRNGKGFEVPISMAFHPIVSVGSGKVFAYEALVRGQDGSGA
AQVFEQVNDDNLYAFDQKCRKTAIEHASRLDLGDTGSLLSINFLPNAVYDPKACIRLTLETAKKTGFPVSQIMFEFTEVE
RISAPHLLDILSTYRRLGFLTSIDDFGAGYAGLDLLSRFQPDVVKLDAELTRNIDQSSVKQTIVRHVISMLEQLGIIIVA
EGIETQGEFSCLYDLGVDLMQGWLFAKPGFETLPDVRWPDALRSVEESKTA
>Mature_291_residues
MLRDKRIALWTRVSSPSCHKEELRDGREMTLTDTEKCKACRNGKGFEVPISMAFHPIVSVGSGKVFAYEALVRGQDGSGA
AQVFEQVNDDNLYAFDQKCRKTAIEHASRLDLGDTGSLLSINFLPNAVYDPKACIRLTLETAKKTGFPVSQIMFEFTEVE
RISAPHLLDILSTYRRLGFLTSIDDFGAGYAGLDLLSRFQPDVVKLDAELTRNIDQSSVKQTIVRHVISMLEQLGIIIVA
EGIETQGEFSCLYDLGVDLMQGWLFAKPGFETLPDVRWPDALRSVEESKTA

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=230, Percent_Identity=36.0869565217391, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1790496, Length=236, Percent_Identity=27.5423728813559, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787541, Length=143, Percent_Identity=34.2657342657343, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87082096, Length=228, Percent_Identity=24.5614035087719, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI226510982, Length=230, Percent_Identity=27.8260869565217, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87081743, Length=232, Percent_Identity=29.7413793103448, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87081980, Length=128, Percent_Identity=34.375, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787055, Length=247, Percent_Identity=28.7449392712551, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788502, Length=230, Percent_Identity=25.2173913043478, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788849, Length=151, Percent_Identity=29.8013245033113, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786507, Length=138, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081845, Length=119, Percent_Identity=31.0924369747899, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081921, Length=252, Percent_Identity=22.2222222222222, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 32403; Mature: 32403

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRDKRIALWTRVSSPSCHKEELRDGREMTLTDTEKCKACRNGKGFEVPISMAFHPIVSV
CCCCCEEEEEEECCCCCCCHHHHCCCCCEEECCHHHHHHHHCCCCCEEEHHHHHHHHHCC
GSGKVFAYEALVRGQDGSGAAQVFEQVNDDNLYAFDQKCRKTAIEHASRLDLGDTGSLLS
CCCCEEEEEHHHCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCEEE
INFLPNAVYDPKACIRLTLETAKKTGFPVSQIMFEFTEVERISAPHLLDILSTYRRLGFL
EEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TSIDDFGAGYAGLDLLSRFQPDVVKLDAELTRNIDQSSVKQTIVRHVISMLEQLGIIIVA
HCHHHHCCCHHHHHHHHHCCCCCEEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEE
EGIETQGEFSCLYDLGVDLMQGWLFAKPGFETLPDVRWPDALRSVEESKTA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLRDKRIALWTRVSSPSCHKEELRDGREMTLTDTEKCKACRNGKGFEVPISMAFHPIVSV
CCCCCEEEEEEECCCCCCCHHHHCCCCCEEECCHHHHHHHHCCCCCEEEHHHHHHHHHCC
GSGKVFAYEALVRGQDGSGAAQVFEQVNDDNLYAFDQKCRKTAIEHASRLDLGDTGSLLS
CCCCEEEEEHHHCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCEEE
INFLPNAVYDPKACIRLTLETAKKTGFPVSQIMFEFTEVERISAPHLLDILSTYRRLGFL
EEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TSIDDFGAGYAGLDLLSRFQPDVVKLDAELTRNIDQSSVKQTIVRHVISMLEQLGIIIVA
HCHHHHCCCHHHHHHHHHCCCCCEEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEE
EGIETQGEFSCLYDLGVDLMQGWLFAKPGFETLPDVRWPDALRSVEESKTA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]