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The map label for this gene is gyrB [H]

Identifier: 99081581

GI number: 99081581

Start: 1834669

End: 1836678

Strand: Direct

Name: gyrB [H]

Synonym: TM1040_1740

Alternate gene names: 99081581

Gene position: 1834669-1836678 (Clockwise)

Preceding gene: 99081576

Following gene: 99081588

Centisome position: 57.32

GC content: 60.15

Gene sequence:

>2010_bases
ATGCGCCATAATGAGATCAAACCACATCAAAGAATGAGCAGCGATCCGCACATGGCAGATGACCTTCTGAGCCCCTCTTC
GTCTGACACCTATGACGCCTCCTCGATCGAGGTTCTCGAGGGCCTCGAACCGGTGCGTCAGCGCCCCGGCATGTATATCG
GTGGCACGGATGAGCGCGCGTTGCATCACATGGTGGCCGAGATCCTCGACAACTCCATGGACGAGGCGGTCGCGGGTCAT
GCCAACCGCATCGAGGTGGAGCTGCACGCGGATTATTCGCTGACCGTGCGCGACAACGGGCGCGGCATTCCAATTGATCC
GCACCCGAAGTTTCCCGACAAATCCGCGCTGGAGGTGATCCTCTGTACGCTGCACGCAGGCGGCAAGTTCTCGGGCAAGG
CCTACCAGACATCGGGCGGCCTGCACGGGGTTGGCTCTTCGGTTGTAAACGCGCTCTCGGATTCGCTGGTGGTTCAGGTG
GCCAAGAACAAGGAATTGTTCGAACAGCGGTTTTCGCGCGGCAAGCCCTTGGGCGGTGTGGAAAAAATCGGCGCTGCCCC
CAATCGGCGCGGCACCACGGTGACATTTCACGCTGATGAGCAGATCTTTGGCTCGCACCGGTTCAAACCGGCGCGCCTGT
TCAAGCTCGTGCGCTCCAAGGCCTATCTTTTTTCGGGCGTCGAGATCCGTTGGAAATCCGCCATTGATGACGGAGAGACC
CCACAGGAAGCGACATTCCATTTCCCTGGTGGTCTGCGCGATTACCTCACCGAAGTGCTGGGCAAATCCTCGGTTTATGC
CGATGCCCCCTTTGCAGGCAAAGTCGAGTTTCGCGAGAAGTTCGGCGAGCCGGGTTATGTGGAATGGGCGATCAACTGGA
CGCCCTCGCGCGACGGGTTCACCCAGTCTTATTGTAACACCGTCCCCACCCCGGAAGGCGGCACCCATGTCGCGGGGTTC
TGGTCCGCGATCCTCAAGGGGATCAAGGCCTATGGCGAGCTGGTCGGCAACAAGAAGGCAGGCCAGATCACCCGCGACGA
CCTGATGGCGGGTGGCTGTGCGCTGGTCTCTTGTTTTATCGCTGACCCGGCGTTTGTGGGCCAGACCAAGGACCGCCTGT
CGACCGAAGCCGCGGCCAAGATGGTTGAAAACTCGGTGCGCGACCATTTCGACAACTGGCTTGCGGCCGATACAAAATCC
GCGGGCGCCATCCTTGATTTCCTGATCCTGCGCGCCGAGGAACGTCTGCGCCGCAGACAGGAAAAGGAAACCGCCCGTAA
ATCCGCCACCAAGAAACTGCGCCTGCCGGGCAAGCTCACGGATTGTACATCCAAGGATCGCTCGGGCACCGAACTGTTCA
TCGTCGAGGGGGACTCGGCCGGTGGCTCCGGCAAAGGCGCGCGCAACCGCGAGTATCAGGCGCTCCTGCCGCTCAAGGGC
AAGATCCTGAACGTGCTCGGTGCGGCCTCGGGCAAGCTTACCTCGAACGCCGAGATCCGAGATCTCTGCGAAGCGCTGGG
GGTGGGGCTAGGAACCAAGTTCAACCTTGATGACCTGCGCTATGACAAGATCATCATCATGACCGATGCGGATGTGGACG
GTGCACATATCGCGTCGCTTCTGATGACCTTTTTCTTTACCCAGATGCGGCCGCTGATTGACGGTGGCCATCTCTATCTC
GCCTGCCCGCCCCTGTTCCGCCTCACCCAGGGGGCCAAGCGCGTCTACTGCCTCGACGAGGCCGAGCGCGACATGTGGAT
GGAAAAAGGTCTGGGCGGCAAAGGCAAGATCGACGTGTCGCGCTTCAAGGGTCTTGGTGAAATGGATGCCAAGGACCTGA
AGGAAACCACGATGGATCCCAAGACCCGCAAGCTGATCCGCGTCACCATCGACGAGGACGAGCCCGGCGAGACTAGCGAC
CTTGTGGAACGCCTGATGGGCAAAAAGCCCGAGCTGCGATTCCAGTACATCCAGGAGAACGCCAAGTTCGTCGAGGAACT
GGACGTTTAA

Upstream 100 bases:

>100_bases
GCGCGCTGGATTTGATCACATTTCGTCAGACCTGTGTTCCACGCCCCCGCAGAGCTCCATTTGCGTTGACCCGCGCAGAC
CTCTTTCCCCGCCCGATGAC

Downstream 100 bases:

>100_bases
GACCCCGTTGGGCAGTGCCTCAATCGCGCTGCCCTATGCCCGCTTTATGCGCATATCTGTGAAAGCACTGTGGGCGCCTG
CGCCGGGGAGCAGTCTGCGC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MRHNEIKPHQRMSSDPHMADDLLSPSSSDTYDASSIEVLEGLEPVRQRPGMYIGGTDERALHHMVAEILDNSMDEAVAGH
ANRIEVELHADYSLTVRDNGRGIPIDPHPKFPDKSALEVILCTLHAGGKFSGKAYQTSGGLHGVGSSVVNALSDSLVVQV
AKNKELFEQRFSRGKPLGGVEKIGAAPNRRGTTVTFHADEQIFGSHRFKPARLFKLVRSKAYLFSGVEIRWKSAIDDGET
PQEATFHFPGGLRDYLTEVLGKSSVYADAPFAGKVEFREKFGEPGYVEWAINWTPSRDGFTQSYCNTVPTPEGGTHVAGF
WSAILKGIKAYGELVGNKKAGQITRDDLMAGGCALVSCFIADPAFVGQTKDRLSTEAAAKMVENSVRDHFDNWLAADTKS
AGAILDFLILRAEERLRRRQEKETARKSATKKLRLPGKLTDCTSKDRSGTELFIVEGDSAGGSGKGARNREYQALLPLKG
KILNVLGAASGKLTSNAEIRDLCEALGVGLGTKFNLDDLRYDKIIIMTDADVDGAHIASLLMTFFFTQMRPLIDGGHLYL
ACPPLFRLTQGAKRVYCLDEAERDMWMEKGLGGKGKIDVSRFKGLGEMDAKDLKETTMDPKTRKLIRVTIDEDEPGETSD
LVERLMGKKPELRFQYIQENAKFVEELDV

Sequences:

>Translated_669_residues
MRHNEIKPHQRMSSDPHMADDLLSPSSSDTYDASSIEVLEGLEPVRQRPGMYIGGTDERALHHMVAEILDNSMDEAVAGH
ANRIEVELHADYSLTVRDNGRGIPIDPHPKFPDKSALEVILCTLHAGGKFSGKAYQTSGGLHGVGSSVVNALSDSLVVQV
AKNKELFEQRFSRGKPLGGVEKIGAAPNRRGTTVTFHADEQIFGSHRFKPARLFKLVRSKAYLFSGVEIRWKSAIDDGET
PQEATFHFPGGLRDYLTEVLGKSSVYADAPFAGKVEFREKFGEPGYVEWAINWTPSRDGFTQSYCNTVPTPEGGTHVAGF
WSAILKGIKAYGELVGNKKAGQITRDDLMAGGCALVSCFIADPAFVGQTKDRLSTEAAAKMVENSVRDHFDNWLAADTKS
AGAILDFLILRAEERLRRRQEKETARKSATKKLRLPGKLTDCTSKDRSGTELFIVEGDSAGGSGKGARNREYQALLPLKG
KILNVLGAASGKLTSNAEIRDLCEALGVGLGTKFNLDDLRYDKIIIMTDADVDGAHIASLLMTFFFTQMRPLIDGGHLYL
ACPPLFRLTQGAKRVYCLDEAERDMWMEKGLGGKGKIDVSRFKGLGEMDAKDLKETTMDPKTRKLIRVTIDEDEPGETSD
LVERLMGKKPELRFQYIQENAKFVEELDV
>Mature_669_residues
MRHNEIKPHQRMSSDPHMADDLLSPSSSDTYDASSIEVLEGLEPVRQRPGMYIGGTDERALHHMVAEILDNSMDEAVAGH
ANRIEVELHADYSLTVRDNGRGIPIDPHPKFPDKSALEVILCTLHAGGKFSGKAYQTSGGLHGVGSSVVNALSDSLVVQV
AKNKELFEQRFSRGKPLGGVEKIGAAPNRRGTTVTFHADEQIFGSHRFKPARLFKLVRSKAYLFSGVEIRWKSAIDDGET
PQEATFHFPGGLRDYLTEVLGKSSVYADAPFAGKVEFREKFGEPGYVEWAINWTPSRDGFTQSYCNTVPTPEGGTHVAGF
WSAILKGIKAYGELVGNKKAGQITRDDLMAGGCALVSCFIADPAFVGQTKDRLSTEAAAKMVENSVRDHFDNWLAADTKS
AGAILDFLILRAEERLRRRQEKETARKSATKKLRLPGKLTDCTSKDRSGTELFIVEGDSAGGSGKGARNREYQALLPLKG
KILNVLGAASGKLTSNAEIRDLCEALGVGLGTKFNLDDLRYDKIIIMTDADVDGAHIASLLMTFFFTQMRPLIDGGHLYL
ACPPLFRLTQGAKRVYCLDEAERDMWMEKGLGGKGKIDVSRFKGLGEMDAKDLKETTMDPKTRKLIRVTIDEDEPGETSD
LVERLMGKKPELRFQYIQENAKFVEELDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=614, Percent_Identity=24.1042345276873, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI19913406, Length=628, Percent_Identity=25.796178343949, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1789408, Length=642, Percent_Identity=42.9906542056075, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI48994957, Length=568, Percent_Identity=41.9014084507042, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17535065, Length=598, Percent_Identity=23.9130434782609, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=607, Percent_Identity=24.0527182866557, Blast_Score=115, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=203, Percent_Identity=27.0935960591133, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=636, Percent_Identity=25.314465408805, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17136538, Length=576, Percent_Identity=25.5208333333333, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73652; Mature: 73652

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHNEIKPHQRMSSDPHMADDLLSPSSSDTYDASSIEVLEGLEPVRQRPGMYIGGTDERA
CCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHH
LHHMVAEILDNSMDEAVAGHANRIEVELHADYSLTVRDNGRGIPIDPHPKFPDKSALEVI
HHHHHHHHHHCCHHHHHCCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCHHHHEEE
LCTLHAGGKFSGKAYQTSGGLHGVGSSVVNALSDSLVVQVAKNKELFEQRFSRGKPLGGV
EEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCH
EKIGAAPNRRGTTVTFHADEQIFGSHRFKPARLFKLVRSKAYLFSGVEIRWKSAIDDGET
HHHCCCCCCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PQEATFHFPGGLRDYLTEVLGKSSVYADAPFAGKVEFREKFGEPGYVEWAINWTPSRDGF
CCCCEEECCCCHHHHHHHHHCCCCCEECCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCC
TQSYCNTVPTPEGGTHVAGFWSAILKGIKAYGELVGNKKAGQITRDDLMAGGCALVSCFI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHHHHHHH
ADPAFVGQTKDRLSTEAAAKMVENSVRDHFDNWLAADTKSAGAILDFLILRAEERLRRRQ
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EKETARKSATKKLRLPGKLTDCTSKDRSGTELFIVEGDSAGGSGKGARNREYQALLPLKG
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCHH
KILNVLGAASGKLTSNAEIRDLCEALGVGLGTKFNLDDLRYDKIIIMTDADVDGAHIASL
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
LMTFFFTQMRPLIDGGHLYLACPPLFRLTQGAKRVYCLDEAERDMWMEKGLGGKGKIDVS
HHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCCCEEHH
RFKGLGEMDAKDLKETTMDPKTRKLIRVTIDEDEPGETSDLVERLMGKKPELRFQYIQEN
HHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
AKFVEELDV
HHHHHHHCC
>Mature Secondary Structure
MRHNEIKPHQRMSSDPHMADDLLSPSSSDTYDASSIEVLEGLEPVRQRPGMYIGGTDERA
CCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHH
LHHMVAEILDNSMDEAVAGHANRIEVELHADYSLTVRDNGRGIPIDPHPKFPDKSALEVI
HHHHHHHHHHCCHHHHHCCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCHHHHEEE
LCTLHAGGKFSGKAYQTSGGLHGVGSSVVNALSDSLVVQVAKNKELFEQRFSRGKPLGGV
EEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCH
EKIGAAPNRRGTTVTFHADEQIFGSHRFKPARLFKLVRSKAYLFSGVEIRWKSAIDDGET
HHHCCCCCCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PQEATFHFPGGLRDYLTEVLGKSSVYADAPFAGKVEFREKFGEPGYVEWAINWTPSRDGF
CCCCEEECCCCHHHHHHHHHCCCCCEECCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCC
TQSYCNTVPTPEGGTHVAGFWSAILKGIKAYGELVGNKKAGQITRDDLMAGGCALVSCFI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHHHHHHH
ADPAFVGQTKDRLSTEAAAKMVENSVRDHFDNWLAADTKSAGAILDFLILRAEERLRRRQ
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EKETARKSATKKLRLPGKLTDCTSKDRSGTELFIVEGDSAGGSGKGARNREYQALLPLKG
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCHH
KILNVLGAASGKLTSNAEIRDLCEALGVGLGTKFNLDDLRYDKIIIMTDADVDGAHIASL
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
LMTFFFTQMRPLIDGGHLYLACPPLFRLTQGAKRVYCLDEAERDMWMEKGLGGKGKIDVS
HHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCCCEEHH
RFKGLGEMDAKDLKETTMDPKTRKLIRVTIDEDEPGETSDLVERLMGKKPELRFQYIQEN
HHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
AKFVEELDV
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]