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The map label for this gene is bam [H]

Identifier: 99081418

GI number: 99081418

Start: 1686460

End: 1687896

Strand: Direct

Name: bam [H]

Synonym: TM1040_1577

Alternate gene names: 99081418

Gene position: 1686460-1687896 (Clockwise)

Preceding gene: 99081415

Following gene: 99081420

Centisome position: 52.69

GC content: 63.67

Gene sequence:

>1437_bases
ATGTCAAATCAAGAGCTTTGGCGCCTGAGCGCAGAAGAATTGTCCACCCAGACCCGCAACGGCGACATCAGTCCAACAGA
GGCGGTGACCTCTGTGCTTGCGCGGATGGATCAGGTGAACCCGGCGCTCAATGCGGTGGTCGAGGATCTCCGGGACGAGG
CTCTGGCGCGGGCCGAAGTTCTCGACAGCACGCCAAAGGCGCTGCGCGGCCCCTTGCACGGCGTCCCTGTCACCATCAAG
ATCAACATCGATCAAACCGGGCATCCGACATCAAACGGCATTCCTGCGCTCAAGGATCTGATCGCCCCCGATGACGCGCC
GGTGGTGCAAAACCTAGAACGCGCCGGAGCTGTGGTGATCGGGCGCAGCAACACGCCAGAGTTTTCCTTCCGCGCCGATA
CCGACAATCCCCTTTATGGGCGCACCCATAATCCCTGGGGGCAGCATATCTCGCCCGGCGGCTCTTCGGGCGGTGGCGGC
GCTGCAGTGATGGCGGGGATTGGTCCGCTGGCACATGGCAATGACATTGGCGGCTCCCTGCGCTTTCCCGCAGCGGCCAA
TGGCGCGGTCACCGTGAAGCCGGGCTTTGGGCGCGTGCCCGCCTGGAACCCCAGCCAAAAAGCCGAACGCGGCCTATTGG
CCCAATTGATGAGCGTTCAAGGCCTGATGACCCGCTCCGCGCGCGATTTGCACCTCTCGATGCCCGCGATGATCGCGCCC
GACATTCGCGACCCCTTCCACGCCCCGGTGCCGTGGCGCGGGGCGGCGCCGGACGGCCCGGTCAAGGTCGCCTTCACCCG
CAACACCCATGGCTATGACCTCCACCCCGAGGTGGACGCGGCGCTCTTGGCGGCGCGCGACGCGCTGATGGATGCCGGGT
ATATCGTCGAGGAGATCGAGCCCCCCAATGTCTTTGAGGCCGGGCGCACAGGCTATCGCGCGCTCATGGGCGAAATTCAT
GCGTTGATGAAACCCGACCTCGACGCCATGGGCTCCGAGATCATCCGCACGATGTTTGACACCTATTTTCAGGAGTTCCC
GCCCTTCCACGGTGATGAGCTGCTCAAGATGTACGCCAAACGCAGCCATTACGCCCGCCAATGGGCCATGTTCATGGCCG
ATTACCCGCTGGTGCTGACGCCCTTCCTGCCGCATCCGTTCTTTGCGCCTGATCGCGATCGCGAAGGTGTCGCGGGCGCC
CATGATGTGCTGGGCTGTGCGGTCTACTCCTACGCCATGAATTACATGGGCCTACCAGCCGCCTGCGTGCCTGCGCGCCT
CGCACAGCTTCCAAAGGGCCTACAACCCATCAACGTGCAGATCGTGGCGCCGCGCTGGCGCGAAGACATGGCCGTGGATG
CCGCTATCGCCATTGAGGATCGCATCGGACAGATGGCTGCCCCGCTCTGGGATCACATGCAGTCCATGACTCGCTGA

Upstream 100 bases:

>100_bases
CACAGGCAGGATCAAGCACAATCCCCAAATAACAAGTTAAATTCCCCCCTTCCCAGCCCCTCAAACGGACACTAGTCTCC
GCCTCAAGGAGATTGTTAAC

Downstream 100 bases:

>100_bases
GCGACGACCTGTGCGCGCCGATGCCTTCTTCTAACCAGAAATATCCCCGCCGGAGGCACGGCCTTGGACCCATGGGTCCA
AGGCTCAACCTGTCGCGCGC

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSNQELWRLSAEELSTQTRNGDISPTEAVTSVLARMDQVNPALNAVVEDLRDEALARAEVLDSTPKALRGPLHGVPVTIK
INIDQTGHPTSNGIPALKDLIAPDDAPVVQNLERAGAVVIGRSNTPEFSFRADTDNPLYGRTHNPWGQHISPGGSSGGGG
AAVMAGIGPLAHGNDIGGSLRFPAAANGAVTVKPGFGRVPAWNPSQKAERGLLAQLMSVQGLMTRSARDLHLSMPAMIAP
DIRDPFHAPVPWRGAAPDGPVKVAFTRNTHGYDLHPEVDAALLAARDALMDAGYIVEEIEPPNVFEAGRTGYRALMGEIH
ALMKPDLDAMGSEIIRTMFDTYFQEFPPFHGDELLKMYAKRSHYARQWAMFMADYPLVLTPFLPHPFFAPDRDREGVAGA
HDVLGCAVYSYAMNYMGLPAACVPARLAQLPKGLQPINVQIVAPRWREDMAVDAAIAIEDRIGQMAAPLWDHMQSMTR

Sequences:

>Translated_478_residues
MSNQELWRLSAEELSTQTRNGDISPTEAVTSVLARMDQVNPALNAVVEDLRDEALARAEVLDSTPKALRGPLHGVPVTIK
INIDQTGHPTSNGIPALKDLIAPDDAPVVQNLERAGAVVIGRSNTPEFSFRADTDNPLYGRTHNPWGQHISPGGSSGGGG
AAVMAGIGPLAHGNDIGGSLRFPAAANGAVTVKPGFGRVPAWNPSQKAERGLLAQLMSVQGLMTRSARDLHLSMPAMIAP
DIRDPFHAPVPWRGAAPDGPVKVAFTRNTHGYDLHPEVDAALLAARDALMDAGYIVEEIEPPNVFEAGRTGYRALMGEIH
ALMKPDLDAMGSEIIRTMFDTYFQEFPPFHGDELLKMYAKRSHYARQWAMFMADYPLVLTPFLPHPFFAPDRDREGVAGA
HDVLGCAVYSYAMNYMGLPAACVPARLAQLPKGLQPINVQIVAPRWREDMAVDAAIAIEDRIGQMAAPLWDHMQSMTR
>Mature_477_residues
SNQELWRLSAEELSTQTRNGDISPTEAVTSVLARMDQVNPALNAVVEDLRDEALARAEVLDSTPKALRGPLHGVPVTIKI
NIDQTGHPTSNGIPALKDLIAPDDAPVVQNLERAGAVVIGRSNTPEFSFRADTDNPLYGRTHNPWGQHISPGGSSGGGGA
AVMAGIGPLAHGNDIGGSLRFPAAANGAVTVKPGFGRVPAWNPSQKAERGLLAQLMSVQGLMTRSARDLHLSMPAMIAPD
IRDPFHAPVPWRGAAPDGPVKVAFTRNTHGYDLHPEVDAALLAARDALMDAGYIVEEIEPPNVFEAGRTGYRALMGEIHA
LMKPDLDAMGSEIIRTMFDTYFQEFPPFHGDELLKMYAKRSHYARQWAMFMADYPLVLTPFLPHPFFAPDRDREGVAGAH
DVLGCAVYSYAMNYMGLPAACVPARLAQLPKGLQPINVQIVAPRWREDMAVDAAIAIEDRIGQMAAPLWDHMQSMTR

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=248, Percent_Identity=34.2741935483871, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI222831590, Length=527, Percent_Identity=24.2884250474383, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI166795287, Length=501, Percent_Identity=25.1497005988024, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=256, Percent_Identity=31.25, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=322, Percent_Identity=26.3975155279503, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=172, Percent_Identity=32.5581395348837, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=512, Percent_Identity=25.1953125, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=197, Percent_Identity=32.994923857868, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=197, Percent_Identity=32.994923857868, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=427, Percent_Identity=25.2927400468384, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=137, Percent_Identity=33.5766423357664, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=235, Percent_Identity=30.2127659574468, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=160, Percent_Identity=35, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=201, Percent_Identity=29.3532338308458, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644968, Length=287, Percent_Identity=33.1010452961672, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI45550774, Length=244, Percent_Identity=33.6065573770492, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648435, Length=244, Percent_Identity=33.6065573770492, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24648437, Length=244, Percent_Identity=33.6065573770492, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21356731, Length=242, Percent_Identity=33.8842975206612, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24652985, Length=507, Percent_Identity=25.0493096646943, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922090, Length=507, Percent_Identity=25.0493096646943, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652981, Length=507, Percent_Identity=25.0493096646943, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652983, Length=507, Percent_Identity=25.0493096646943, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648113, Length=512, Percent_Identity=26.171875, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078093, Length=502, Percent_Identity=25.0996015936255, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=35.8823529411765, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=35.8823529411765, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 51759; Mature: 51627

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQELWRLSAEELSTQTRNGDISPTEAVTSVLARMDQVNPALNAVVEDLRDEALARAEV
CCCCHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LDSTPKALRGPLHGVPVTIKINIDQTGHPTSNGIPALKDLIAPDDAPVVQNLERAGAVVI
HCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCEEEE
GRSNTPEFSFRADTDNPLYGRTHNPWGQHISPGGSSGGGGAAVMAGIGPLAHGNDIGGSL
ECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCE
RFPAAANGAVTVKPGFGRVPAWNPSQKAERGLLAQLMSVQGLMTRSARDLHLSMPAMIAP
ECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCC
DIRDPFHAPVPWRGAAPDGPVKVAFTRNTHGYDLHPEVDAALLAARDALMDAGYIVEEIE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCC
PPNVFEAGRTGYRALMGEIHALMKPDLDAMGSEIIRTMFDTYFQEFPPFHGDELLKMYAK
CCCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
RSHYARQWAMFMADYPLVLTPFLPHPFFAPDRDREGVAGAHDVLGCAVYSYAMNYMGLPA
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
ACVPARLAQLPKGLQPINVQIVAPRWREDMAVDAAIAIEDRIGQMAAPLWDHMQSMTR
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNQELWRLSAEELSTQTRNGDISPTEAVTSVLARMDQVNPALNAVVEDLRDEALARAEV
CCCHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LDSTPKALRGPLHGVPVTIKINIDQTGHPTSNGIPALKDLIAPDDAPVVQNLERAGAVVI
HCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCEEEE
GRSNTPEFSFRADTDNPLYGRTHNPWGQHISPGGSSGGGGAAVMAGIGPLAHGNDIGGSL
ECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCE
RFPAAANGAVTVKPGFGRVPAWNPSQKAERGLLAQLMSVQGLMTRSARDLHLSMPAMIAP
ECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCC
DIRDPFHAPVPWRGAAPDGPVKVAFTRNTHGYDLHPEVDAALLAARDALMDAGYIVEEIE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCC
PPNVFEAGRTGYRALMGEIHALMKPDLDAMGSEIIRTMFDTYFQEFPPFHGDELLKMYAK
CCCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
RSHYARQWAMFMADYPLVLTPFLPHPFFAPDRDREGVAGAHDVLGCAVYSYAMNYMGLPA
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
ACVPARLAQLPKGLQPINVQIVAPRWREDMAVDAAIAIEDRIGQMAAPLWDHMQSMTR
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]