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The map label for this gene is prpE [C]

Identifier: 99081415

GI number: 99081415

Start: 1683467

End: 1685359

Strand: Direct

Name: prpE [C]

Synonym: TM1040_1574

Alternate gene names: 99081415

Gene position: 1683467-1685359 (Clockwise)

Preceding gene: 99081414

Following gene: 99081418

Centisome position: 52.59

GC content: 61.91

Gene sequence:

>1893_bases
ATGACCTATTCTGAGGTATATGCGCAGTGGAAAGCCGATCCAGAGGGGTTCTGGATGGAGGCGGCCGGCGCCATCAGCTG
GGACAAAGCCCCGTCAAAGGCGCTGAGCGATCTGGGCGGTGGGCTCTATGAGTGGTTTGCCGACAGCAAGGTCAACACCT
GCTACAACGCGGTGGACCGTCATGTTGAGGCCGGGCGCGGCGAGCAGACCGCGATCATCTACGACAGCCCCGTCACCCAC
ACAAAACGCGAGATTTCCTTTGTCGAGCTGCGCAACCGCGTGGCCACCCTCGCCGGTGCGTTGCGCGCCAAGGGGGTCGA
AAAAGGCGATCGCGTCATCATCTACATGCCGATGATCCCCGAAGCGCTGGAAGCCATGCTCGCCTGTGCGCGGATCGGCG
CGGTGCATTCGGTGGTCTTTGGCGGCTTTGCCTCCTCGGAGCTTGCGGTGCGGATTGACGACGCCACCCCCAAGGCGATC
ATCGCCGCCTCCTGCGGGATCGAGCCGGGACGTATCGTCCATTACAAGCCGCTTCTGGATGGAGCCATCGACATGGCCAG
CCACAAGCCGGACTTCTGCGTGATCCTCCAGCGCGAGCAGGAAGTTGCGGAGCTTGTCGAGGGGCGCGACGTGAACTGGC
ACGGGTTCCAATATGGCGTCGAGCCTGCAGACTGTGTGCCGGTCGAGGGCAATCATCCCTCCTATATCCTCTATACCTCC
GGGACCACCGGACAGCCCAAGGGGGTGATCCGCCACACGGCCGGGCAGCTTGTGGCGCTCAATTGGTCTATGAAGAACAT
CTATAACGTCGATCCGGGCGATGTGTTCTGGGCCGCGTCCGACGTGGGCTGGGTCGTGGGACACAGCTATATCTGCTATG
GACCGCTCATCCATGGCAACACCACCGTTGTCTTTGAGGGCAAGCCCGTGGGCACGCCTGATGCGGGCACCTTCTGGCGG
GTGATCTCGGAACATAAGGTCAAAAGCTTCTTCACCGCGCCCACCGCATTCCGCGCCGTCAAACGCGAAGATCCCAAAGG
CGAGTTTGTTGGGAAATACGATCTTTCCTGTCTGGAGCAGGTCTATCTCGCGGGCGAGCGCGCAGATCCCGACACGATCA
TCTGGGCGCAGGACCAACTGGGCGTGCCAGTCATCGATCACTGGTGGCAGACAGAAACCGGCTGGTCGATTGCTGCCAAT
CCGCTTGGTATTGAAACGCTCCCGGTGAAGCTCGGCTCCCCCGCCGTGCCAATGCCCGGCTATGACGTGCAGATCCTTGA
CGAGGGGGGCCATCCTGTCGCCCCCGGAGAGCTGGGTGCGATTGCGGTCAAACTCCCCCTGCCCCCAGGCACGCTGCCAA
CGCTCTGGAACGCCGAAGAGCGGTTCAAGAAAAGCTACCTCACCACCTTTCCTGGCTACTACGAGACGGGCGATGCAGGG
ATGATGGATGAGGATGGGTATCTCTACATCATGGCGCGCACCGATGACGTCATCAACGTGGCCGGACATCGCCTGTCTAC
AGGCGCGATGGAAGAGGTGCTTGCGGGCCACCCGGATGTTGCAGAATGCGCCGTGATCGGCGTGGCAGACCAACTGAAGG
GGCAGATGCCCCTGGGCTTCCTCTGCCTCAACTCCGGCTGCAATCGTCCTCATTCCGAGATCACCGCCGAGGTGGTCAAA
CTGGTGCGCGAAAAGATCGGCCCCGTGGCGGCGTTCAAACTCGCCTGCGTGGTGGATCGCCTCCCCAAGACGCGCTCGGG
CAAGATCCTGCGGGGCACCATGGTCTCCATCGCGGACAACACCGAGTGGAAGATGCCCGCGACCATCGATGATCCGGCCA
TCCTGGACGAGATCAAAACCGCCCTCCAAAGCCTCGGCTACGCCCAAGGGTAG

Upstream 100 bases:

>100_bases
AACAGGTTCTAATTCGTCGCAGCAGCCGTCGCCGCGCTTGCGAGAGCTTTGCTCTTGCGCCTAGGCTCTGTCCTCAGAAC
AGACAGGGAGGAGGACCCCG

Downstream 100 bases:

>100_bases
GACAGAACAAAGAAAAGCGGCCCGCCTTGGGGCCGCTTCCTCGTCCACGCTGCGATGAGCGCGGTGCTCGTATCAGATCT
TTTCCTTCAGGCTCTCTGCA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MTYSEVYAQWKADPEGFWMEAAGAISWDKAPSKALSDLGGGLYEWFADSKVNTCYNAVDRHVEAGRGEQTAIIYDSPVTH
TKREISFVELRNRVATLAGALRAKGVEKGDRVIIYMPMIPEALEAMLACARIGAVHSVVFGGFASSELAVRIDDATPKAI
IAASCGIEPGRIVHYKPLLDGAIDMASHKPDFCVILQREQEVAELVEGRDVNWHGFQYGVEPADCVPVEGNHPSYILYTS
GTTGQPKGVIRHTAGQLVALNWSMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGNTTVVFEGKPVGTPDAGTFWR
VISEHKVKSFFTAPTAFRAVKREDPKGEFVGKYDLSCLEQVYLAGERADPDTIIWAQDQLGVPVIDHWWQTETGWSIAAN
PLGIETLPVKLGSPAVPMPGYDVQILDEGGHPVAPGELGAIAVKLPLPPGTLPTLWNAEERFKKSYLTTFPGYYETGDAG
MMDEDGYLYIMARTDDVINVAGHRLSTGAMEEVLAGHPDVAECAVIGVADQLKGQMPLGFLCLNSGCNRPHSEITAEVVK
LVREKIGPVAAFKLACVVDRLPKTRSGKILRGTMVSIADNTEWKMPATIDDPAILDEIKTALQSLGYAQG

Sequences:

>Translated_630_residues
MTYSEVYAQWKADPEGFWMEAAGAISWDKAPSKALSDLGGGLYEWFADSKVNTCYNAVDRHVEAGRGEQTAIIYDSPVTH
TKREISFVELRNRVATLAGALRAKGVEKGDRVIIYMPMIPEALEAMLACARIGAVHSVVFGGFASSELAVRIDDATPKAI
IAASCGIEPGRIVHYKPLLDGAIDMASHKPDFCVILQREQEVAELVEGRDVNWHGFQYGVEPADCVPVEGNHPSYILYTS
GTTGQPKGVIRHTAGQLVALNWSMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGNTTVVFEGKPVGTPDAGTFWR
VISEHKVKSFFTAPTAFRAVKREDPKGEFVGKYDLSCLEQVYLAGERADPDTIIWAQDQLGVPVIDHWWQTETGWSIAAN
PLGIETLPVKLGSPAVPMPGYDVQILDEGGHPVAPGELGAIAVKLPLPPGTLPTLWNAEERFKKSYLTTFPGYYETGDAG
MMDEDGYLYIMARTDDVINVAGHRLSTGAMEEVLAGHPDVAECAVIGVADQLKGQMPLGFLCLNSGCNRPHSEITAEVVK
LVREKIGPVAAFKLACVVDRLPKTRSGKILRGTMVSIADNTEWKMPATIDDPAILDEIKTALQSLGYAQG
>Mature_629_residues
TYSEVYAQWKADPEGFWMEAAGAISWDKAPSKALSDLGGGLYEWFADSKVNTCYNAVDRHVEAGRGEQTAIIYDSPVTHT
KREISFVELRNRVATLAGALRAKGVEKGDRVIIYMPMIPEALEAMLACARIGAVHSVVFGGFASSELAVRIDDATPKAII
AASCGIEPGRIVHYKPLLDGAIDMASHKPDFCVILQREQEVAELVEGRDVNWHGFQYGVEPADCVPVEGNHPSYILYTSG
TTGQPKGVIRHTAGQLVALNWSMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGNTTVVFEGKPVGTPDAGTFWRV
ISEHKVKSFFTAPTAFRAVKREDPKGEFVGKYDLSCLEQVYLAGERADPDTIIWAQDQLGVPVIDHWWQTETGWSIAANP
LGIETLPVKLGSPAVPMPGYDVQILDEGGHPVAPGELGAIAVKLPLPPGTLPTLWNAEERFKKSYLTTFPGYYETGDAGM
MDEDGYLYIMARTDDVINVAGHRLSTGAMEEVLAGHPDVAECAVIGVADQLKGQMPLGFLCLNSGCNRPHSEITAEVVKL
VREKIGPVAAFKLACVVDRLPKTRSGKILRGTMVSIADNTEWKMPATIDDPAILDEIKTALQSLGYAQG

Specific function: Catalyzes The Synthesis Of Propionyl-CoA From Propionate And CoA. Also Converts Acetate To Acetyl-CoA But With A Lower Specific Activity (By Similarity). [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=626, Percent_Identity=51.9169329073482, Blast_Score=665, Evalue=0.0,
Organism=Homo sapiens, GI8923896, Length=655, Percent_Identity=36.030534351145, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI253970489, Length=668, Percent_Identity=35.4790419161677, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI28416953, Length=637, Percent_Identity=35.7927786499215, Blast_Score=349, Evalue=6e-96,
Organism=Homo sapiens, GI38505220, Length=520, Percent_Identity=29.2307692307692, Blast_Score=184, Evalue=3e-46,
Organism=Homo sapiens, GI42544132, Length=522, Percent_Identity=27.7777777777778, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI122937307, Length=524, Percent_Identity=27.8625954198473, Blast_Score=171, Evalue=3e-42,
Organism=Homo sapiens, GI58082049, Length=528, Percent_Identity=27.0833333333333, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI157311624, Length=528, Percent_Identity=26.8939393939394, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI157311622, Length=528, Percent_Identity=26.8939393939394, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=25.8188824662813, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI31982927, Length=601, Percent_Identity=23.4608985024958, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI195947378, Length=411, Percent_Identity=26.0340632603406, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI42544134, Length=342, Percent_Identity=25.7309941520468, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI187761345, Length=407, Percent_Identity=26.7813267813268, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI187761343, Length=407, Percent_Identity=26.7813267813268, Blast_Score=93, Evalue=8e-19,
Organism=Escherichia coli, GI1786529, Length=633, Percent_Identity=45.9715639810426, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1790505, Length=633, Percent_Identity=37.914691943128, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1786801, Length=519, Percent_Identity=25.2408477842004, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI145693145, Length=510, Percent_Identity=22.5490196078431, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI221142682, Length=535, Percent_Identity=21.1214953271028, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788107, Length=559, Percent_Identity=23.2558139534884, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786810, Length=519, Percent_Identity=21.5799614643545, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=624, Percent_Identity=34.9358974358974, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI71983001, Length=624, Percent_Identity=34.9358974358974, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17559526, Length=556, Percent_Identity=22.1223021582734, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=555, Percent_Identity=24.3243243243243, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=555, Percent_Identity=23.4234234234234, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=630, Percent_Identity=22.2222222222222, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=538, Percent_Identity=21.5613382899628, Blast_Score=83, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=130, Percent_Identity=32.3076923076923, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71996755, Length=195, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323182, Length=656, Percent_Identity=36.7378048780488, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6319264, Length=638, Percent_Identity=35.5799373040752, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319699, Length=418, Percent_Identity=25.3588516746411, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355225, Length=634, Percent_Identity=46.3722397476341, Blast_Score=610, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24667955, Length=628, Percent_Identity=37.4203821656051, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI62472339, Length=539, Percent_Identity=39.3320964749536, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24667959, Length=506, Percent_Identity=39.3280632411067, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24648260, Length=378, Percent_Identity=26.4550264550265, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355181, Length=533, Percent_Identity=23.6397748592871, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356441, Length=375, Percent_Identity=26.6666666666667, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI18859661, Length=558, Percent_Identity=23.1182795698925, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648253, Length=523, Percent_Identity=24.4741873804971, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281365686, Length=370, Percent_Identity=22.4324324324324, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356947, Length=525, Percent_Identity=20.7619047619048, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653035, Length=371, Percent_Identity=22.911051212938, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.17 [C]

Molecular weight: Translated: 68418; Mature: 68286

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSEVYAQWKADPEGFWMEAAGAISWDKAPSKALSDLGGGLYEWFADSKVNTCYNAVDR
CCHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHH
HVEAGRGEQTAIIYDSPVTHTKREISFVELRNRVATLAGALRAKGVEKGDRVIIYMPMIP
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCH
EALEAMLACARIGAVHSVVFGGFASSELAVRIDDATPKAIIAASCGIEPGRIVHYKPLLD
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEECCHHC
GAIDMASHKPDFCVILQREQEVAELVEGRDVNWHGFQYGVEPADCVPVEGNHPSYILYTS
CHHHHHCCCCCEEEEEECHHHHHHHHCCCCCCCCCEEECCCCCCEEECCCCCCCEEEEEC
GTTGQPKGVIRHTAGQLVALNWSMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGN
CCCCCCCCHHEECCCCEEEEEECHHHCCCCCCCCEEEEECCCCHHHCCCEEEECCEEECC
TTVVFEGKPVGTPDAGTFWRVISEHKVKSFFTAPTAFRAVKREDPKGEFVGKYDLSCLEQ
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEECCCHHHHHH
VYLAGERADPDTIIWAQDQLGVPVIDHWWQTETGWSIAANPLGIETLPVKLGSPAVPMPG
HHHCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCC
YDVQILDEGGHPVAPGELGAIAVKLPLPPGTLPTLWNAEERFKKSYLTTFPGYYETGDAG
CCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCC
MMDEDGYLYIMARTDDVINVAGHRLSTGAMEEVLAGHPDVAECAVIGVADQLKGQMPLGF
CCCCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHCCCCCHHHEEEEEEHHHHCCCCCEEE
LCLNSGCNRPHSEITAEVVKLVREKIGPVAAFKLACVVDRLPKTRSGKILRGTMVSIADN
EEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECC
TEWKMPATIDDPAILDEIKTALQSLGYAQG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TYSEVYAQWKADPEGFWMEAAGAISWDKAPSKALSDLGGGLYEWFADSKVNTCYNAVDR
CHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHH
HVEAGRGEQTAIIYDSPVTHTKREISFVELRNRVATLAGALRAKGVEKGDRVIIYMPMIP
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCH
EALEAMLACARIGAVHSVVFGGFASSELAVRIDDATPKAIIAASCGIEPGRIVHYKPLLD
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEECCHHC
GAIDMASHKPDFCVILQREQEVAELVEGRDVNWHGFQYGVEPADCVPVEGNHPSYILYTS
CHHHHHCCCCCEEEEEECHHHHHHHHCCCCCCCCCEEECCCCCCEEECCCCCCCEEEEEC
GTTGQPKGVIRHTAGQLVALNWSMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGN
CCCCCCCCHHEECCCCEEEEEECHHHCCCCCCCCEEEEECCCCHHHCCCEEEECCEEECC
TTVVFEGKPVGTPDAGTFWRVISEHKVKSFFTAPTAFRAVKREDPKGEFVGKYDLSCLEQ
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEECCCHHHHHH
VYLAGERADPDTIIWAQDQLGVPVIDHWWQTETGWSIAANPLGIETLPVKLGSPAVPMPG
HHHCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCC
YDVQILDEGGHPVAPGELGAIAVKLPLPPGTLPTLWNAEERFKKSYLTTFPGYYETGDAG
CCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCC
MMDEDGYLYIMARTDDVINVAGHRLSTGAMEEVLAGHPDVAECAVIGVADQLKGQMPLGF
CCCCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHCCCCCHHHEEEEEEHHHHCCCCCEEE
LCLNSGCNRPHSEITAEVVKLVREKIGPVAAFKLACVVDRLPKTRSGKILRGTMVSIADN
EEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECC
TEWKMPATIDDPAILDEIKTALQSLGYAQG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 1339433 [H]