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The map label for this gene is fadA [H]

Identifier: 99081402

GI number: 99081402

Start: 1666096

End: 1667307

Strand: Direct

Name: fadA [H]

Synonym: TM1040_1561

Alternate gene names: 99081402

Gene position: 1666096-1667307 (Clockwise)

Preceding gene: 99081401

Following gene: 99081403

Centisome position: 52.05

GC content: 60.4

Gene sequence:

>1212_bases
ATGACCGAAGCTTATATCTATGATGCCGTTCGCACCCCGCGCGGCAAGGGCCGCAAGGACGGCGCCCTCCACGAGGTGAC
CTCTGTGCGCCTCTCTGCCCTGACCCTGAACGCGCTGAAAGAACGCAACAACCTGGAAGGCCATGCGGTCGAGGATGTGA
TCTGGGGCAATGTCACGCAGGTGATGGAACAGGGTGGCTGTCTGGCGCGCTCTGCCGTGCTGGCCTCCGATCTGGATCAA
TCCATCCCGGGTCTTGCGATCAACCGCTTCTGCGCCTCTGGCATGGAAGCCGTGAACCTCGCTGCAAACCAAGTCAAAGG
TGGCGCAGGCGATGGCTATATCGCGGGCGGCGTCGAGATGATGGGCCGTGTGGCAATGGGATCGGACGGGGCGGCGATTG
CCGTGGACCCGAGCCTTGCGATGGACACGTATTTTGTGCCGCAGGGGATCTCGGCCGATATCATCGCAACCGAGTATGGG
TTTACCCGCGATCAGGCCGACGCACTGGCGGTGGAATCCCAGCGCCGTGCCAAGGCCGCGTGGGATGACAATCGCTTTGC
CAAGTCGATCATCACCGTACGCGACCAGAACGGTTTGGCGATCCTTGATCATGACGAATACATGCGCCCCGGCACCGATA
TGCAGGCGCTTGGATCGCTCAATGCATCGTTTCAGATGATGGGCGAAGTAATGCCTGGCTTTGATGCCGTGGCAAAACTG
AAGTACCCGCATCTGGAAAAGGTCAACCACATCCACCACGCCGGGAACTCCTCAGGGATCGTGGATGGCTCTGCCGCGCT
GTTGATCGGCAACAAGGAATTTGGCGAACGCCATGGTCTGAAGCCGCGCGCCCGTATCAAGGCAACCGCCAAGATCGGCA
CCGACCCGACCATCATGCTGACCGGTCCTGTGCCTGTGACCGAGAAGATCCTCAAAGACACCGGCATGACAATATCCGAT
CTGGATCTATTTGAGGTCAACGAGGCCTTTGCCTCCGTGGTGTTGCGCTTCCAGCAAGCCTTTGACGTCGATCCGGCCCT
GGTAAACGTGAACGGCGGCTCCATCGCCATGGGGCACCCTTTGGGGGCAACCGGTGCGATCATCATCGGCACGCTGCTGG
ACGAGCTGGAGCGGCAGGACAAGGAATGCGGCCTTGCCACGCTCTGCATTGCATCCGGCATGGGTGCGGCCACCATCATC
GAGCGCGTCTAA

Upstream 100 bases:

>100_bases
ATCGACGCCTGGCTGTCGCGCCTCAGCGCCCTGCCCGGCTGGAAACACCCCTATGACCTGATGCCCGGCAACCCGTCGGA
TCGAGCTTGAGGAGACCAAT

Downstream 100 bases:

>100_bases
GCGACGCCTTCTCACCTGATAGGGTTGGAGACAAAGACTATGACCGATTTCAAATACGACGTAGACGCCGATGGCATCGC
CATCATCACATGGGACGCGG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTEAYIYDAVRTPRGKGRKDGALHEVTSVRLSALTLNALKERNNLEGHAVEDVIWGNVTQVMEQGGCLARSAVLASDLDQ
SIPGLAINRFCASGMEAVNLAANQVKGGAGDGYIAGGVEMMGRVAMGSDGAAIAVDPSLAMDTYFVPQGISADIIATEYG
FTRDQADALAVESQRRAKAAWDDNRFAKSIITVRDQNGLAILDHDEYMRPGTDMQALGSLNASFQMMGEVMPGFDAVAKL
KYPHLEKVNHIHHAGNSSGIVDGSAALLIGNKEFGERHGLKPRARIKATAKIGTDPTIMLTGPVPVTEKILKDTGMTISD
LDLFEVNEAFASVVLRFQQAFDVDPALVNVNGGSIAMGHPLGATGAIIIGTLLDELERQDKECGLATLCIASGMGAATII
ERV

Sequences:

>Translated_403_residues
MTEAYIYDAVRTPRGKGRKDGALHEVTSVRLSALTLNALKERNNLEGHAVEDVIWGNVTQVMEQGGCLARSAVLASDLDQ
SIPGLAINRFCASGMEAVNLAANQVKGGAGDGYIAGGVEMMGRVAMGSDGAAIAVDPSLAMDTYFVPQGISADIIATEYG
FTRDQADALAVESQRRAKAAWDDNRFAKSIITVRDQNGLAILDHDEYMRPGTDMQALGSLNASFQMMGEVMPGFDAVAKL
KYPHLEKVNHIHHAGNSSGIVDGSAALLIGNKEFGERHGLKPRARIKATAKIGTDPTIMLTGPVPVTEKILKDTGMTISD
LDLFEVNEAFASVVLRFQQAFDVDPALVNVNGGSIAMGHPLGATGAIIIGTLLDELERQDKECGLATLCIASGMGAATII
ERV
>Mature_402_residues
TEAYIYDAVRTPRGKGRKDGALHEVTSVRLSALTLNALKERNNLEGHAVEDVIWGNVTQVMEQGGCLARSAVLASDLDQS
IPGLAINRFCASGMEAVNLAANQVKGGAGDGYIAGGVEMMGRVAMGSDGAAIAVDPSLAMDTYFVPQGISADIIATEYGF
TRDQADALAVESQRRAKAAWDDNRFAKSIITVRDQNGLAILDHDEYMRPGTDMQALGSLNASFQMMGEVMPGFDAVAKLK
YPHLEKVNHIHHAGNSSGIVDGSAALLIGNKEFGERHGLKPRARIKATAKIGTDPTIMLTGPVPVTEKILKDTGMTISDL
DLFEVNEAFASVVLRFQQAFDVDPALVNVNGGSIAMGHPLGATGAIIIGTLLDELERQDKECGLATLCIASGMGAATIIE
RV

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=411, Percent_Identity=32.1167883211679, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI4501853, Length=391, Percent_Identity=32.9923273657289, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI148539872, Length=404, Percent_Identity=31.9306930693069, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI4557237, Length=418, Percent_Identity=31.3397129186603, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=27.4157303370787, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI194353979, Length=121, Percent_Identity=41.3223140495868, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI48994986, Length=412, Percent_Identity=38.8349514563107, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1787663, Length=427, Percent_Identity=36.768149882904, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788554, Length=418, Percent_Identity=36.6028708133971, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI87082165, Length=418, Percent_Identity=35.8851674641148, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788683, Length=428, Percent_Identity=28.0373831775701, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI133906874, Length=413, Percent_Identity=33.6561743341404, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI25147385, Length=413, Percent_Identity=29.7820823244552, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17535921, Length=418, Percent_Identity=27.2727272727273, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=27.970297029703, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17551802, Length=450, Percent_Identity=26.2222222222222, Blast_Score=118, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=31.7617866004963, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=418, Percent_Identity=30.3827751196172, Blast_Score=153, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24655093, Length=418, Percent_Identity=33.7320574162679, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17648125, Length=414, Percent_Identity=31.8840579710145, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24640423, Length=417, Percent_Identity=30.4556354916067, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI17137578, Length=446, Percent_Identity=26.2331838565022, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42681; Mature: 42550

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAYIYDAVRTPRGKGRKDGALHEVTSVRLSALTLNALKERNNLEGHAVEDVIWGNVTQ
CCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHH
VMEQGGCLARSAVLASDLDQSIPGLAINRFCASGMEAVNLAANQVKGGAGDGYIAGGVEM
HHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCHHH
MGRVAMGSDGAAIAVDPSLAMDTYFVPQGISADIIATEYGFTRDQADALAVESQRRAKAA
HHHHEECCCCCEEEECCCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHC
WDDNRFAKSIITVRDQNGLAILDHDEYMRPGTDMQALGSLNASFQMMGEVMPGFDAVAKL
CCCHHHHHHEEEEECCCCEEEEECHHHCCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHC
KYPHLEKVNHIHHAGNSSGIVDGSAALLIGNKEFGERHGLKPRARIKATAKIGTDPTIML
CCCCHHHHHHHHHCCCCCCEECCCEEEEECCHHHHHHCCCCCCHHEEEEEECCCCCEEEE
TGPVPVTEKILKDTGMTISDLDLFEVNEAFASVVLRFQQAFDVDPALVNVNGGSIAMGHP
ECCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCC
LGATGAIIIGTLLDELERQDKECGLATLCIASGMGAATIIERV
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TEAYIYDAVRTPRGKGRKDGALHEVTSVRLSALTLNALKERNNLEGHAVEDVIWGNVTQ
CCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHH
VMEQGGCLARSAVLASDLDQSIPGLAINRFCASGMEAVNLAANQVKGGAGDGYIAGGVEM
HHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCHHH
MGRVAMGSDGAAIAVDPSLAMDTYFVPQGISADIIATEYGFTRDQADALAVESQRRAKAA
HHHHEECCCCCEEEECCCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHC
WDDNRFAKSIITVRDQNGLAILDHDEYMRPGTDMQALGSLNASFQMMGEVMPGFDAVAKL
CCCHHHHHHEEEEECCCCEEEEECHHHCCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHC
KYPHLEKVNHIHHAGNSSGIVDGSAALLIGNKEFGERHGLKPRARIKATAKIGTDPTIML
CCCCHHHHHHHHHCCCCCCEECCCEEEEECCHHHHHHCCCCCCHHEEEEEECCCCCEEEE
TGPVPVTEKILKDTGMTISDLDLFEVNEAFASVVLRFQQAFDVDPALVNVNGGSIAMGHP
ECCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCC
LGATGAIIIGTLLDELERQDKECGLATLCIASGMGAATIIERV
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]