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The map label for this gene is fadJ [H]

Identifier: 99081403

GI number: 99081403

Start: 1667347

End: 1669548

Strand: Direct

Name: fadJ [H]

Synonym: TM1040_1562

Alternate gene names: 99081403

Gene position: 1667347-1669548 (Clockwise)

Preceding gene: 99081402

Following gene: 99081406

Centisome position: 52.09

GC content: 61.53

Gene sequence:

>2202_bases
ATGACCGATTTCAAATACGACGTAGACGCCGATGGCATCGCCATCATCACATGGGACGCGGAAGGCAAGAGCATGAACGT
CCTCACCCGCGAGGCCTTTGGCCTGGTGGAGGACTATATCGACCGTGCGCTTGGCGATGACGCCGTCAAAGGCGTGGTGA
TCACTTCCGGCAAGAAAGACTTTGCGGGCGGCATGGACCTGAATGTGTTGGCCACCATCCGCGAGGAATCCGGTGAAAAC
CCGGCTCAGGGCCTGTTTGATTTTACGATGAATGGCCACCGCATCCTGCGCAAGCTGGAGCGCGCAGGGATGGACGCCAA
GAACAACAAGGGCGGCAAGCCGATTGCCTGCGCCATCAATGGCACCTGTGCAGGCATCGGCACCGAGATCGCGCTGGCCT
GTCATTACCGAACCATGACCGACAACCCAAAGGCCAAGATCGGCCTGCCGGAGATCCTGCTGGGCATCTTCCCCGGTGGC
GGCGGTACCATCCGCTATTCGCGCATGGTGGGTGCCGTGAACGCCGCCCCCGTGCTGCTTGAAGGCAAGATGATGGACCC
CAAGAAGGCGAAATCCGCGAGCCTTGTGGATGAGGTTGCCGCCGATCCGCTGGAAGCGGCGCGTCAGTGGGTGCTCAACG
CCAAAGACGCCGATATCGTGAAGCCCTGGGATCAGAAGGGCTACAAAATGCCCGGTGGTGCGCCCTATCACCCGGCGGGG
TTCATGAACTTTGTCGGCGCGTCTGCCATGGTGCATGGCAAGACCCAAGGCGCCTTCCCGGCGGCCAAGGCGCTGCTCTC
GGCGGTCTATGAGGGCGCATTGGTGGATTTTGACACCGCGCTGCGCATCGAGGCGCGCTGGTTCACCCATGTTCTGATGA
ACCCGTCGTCCTCTGCGATGATCCGCTCGCTGTTCCTCAACAAGCAAGCGCTGGAGAAAGGCGCCGTGCGGCCCAAGGGC
ATTGAGGATCAGTCCGTGAAGAAGATCGGCGTGCTCGGCGCAGGCATGATGGGCGCAGGCATTGCACTGGTTTCAGCGCA
GGCGGGCATGGAGGTCGTACTGATCGACCGCGATCAGGACGCCGCCGACAAAGGCAAGGCCTACTCTGCCACCTATATGG
ACAAGGGCATCAAGCGCGGCAAAGCCACGCCGGAGAAAAAAGAGGCGCTGCTGGCGCAGATCACCGCAACAGCAGATCTC
GACGCCCTCAAAGGCTGCGATCTCATCATTGAGGCGGTGTTTGAAGACCCGGGCGTCAAGGCCGAGATGACCAAGAAGGT
CGAGGCGATCATCCCCGAGGATTGCATCTTTGCCTCCAACACCTCGACCCTGCCGATCACCAGCCTGGCCGAGGCCTCGG
TCCGGCCCGAGCAGTTCATCGGCATTCACTTCTTCTCTCCGGTTGAGAAGATGTTCCTGGTGGAGATCATCAAGGGTGAG
AAAACCGGCGATCGCGCGGTGGCCAAGGCGCTGGACTATGTACGCCAGATCCGCAAGACGCCGATCGTGGTCAATGATGC
GCGCTTCTTCTACTGCAACCGCTGCATCATCCCCTACATCAACGAGGGCGCAAGGATGATCACCGAAGGTGTCTCGCCGG
TGCTGATCGACAACGCCGCGCGCCAGCTTGGCTTCCCGGTGGGGCCGATCCAGCTGACGGATGAGACCTCGATCGATCTC
GGTGCAAAGATCGCACGCGCCACCAAGGCGGCGATGGGCGACGCCTATCCCGAAAGCCCGGCGGACGATCTGATCTTCTG
GATGGAAGATCAGGGGCGTCTGGGGCGCAAGGCCAATGCGGGCTTCTTTGACTATGACGACAAGGGCAAGCGCGTTGAGT
ACTGGAAGGGGCTTGCGGAGAAATACCCGCTGGCGGAGGTTCAGCCCGATCTGGTTGAGGTCCAGGAGCGGCTGATGTTT
GCGCAGGTCCTTGAGGCTGTGCGGGCACTGGAGGAAAACGTGCTGATGGACATCCGCGAGGGTGACGTGGGCGCGGTTCT
CGCCTGGGGCTTTGCGCCGTGGTCCGGTGGACCGCTCAGCTGGCTTGACATCATCGGCACGCCCTATGCAGCCGAGCGTT
GTGACCAACTGGCCGAGGCATACGGCGACCGTTTTGCCTGCCCGCCGCTCCTGCGGGAGATGGCCGAAAAAGGCCAGACC
TTCTATGGCCGGTTTGCGCCGGAAGAGGATCAAGCCGCCTAA

Upstream 100 bases:

>100_bases
CCTTGCCACGCTCTGCATTGCATCCGGCATGGGTGCGGCCACCATCATCGAGCGCGTCTAAGCGACGCCTTCTCACCTGA
TAGGGTTGGAGACAAAGACT

Downstream 100 bases:

>100_bases
ACCGTCCTGCCTTCATGCTGATATGGCCGCCCCTGTCTTTGGGGCGGCTATTTTTTTTGCGAGGCTCTGCCTCGCGCTCA
GAGATATTTGAGAAAAGATG

Product: 3-hydroxyacyl-CoA dehydrogenase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MTDFKYDVDADGIAIITWDAEGKSMNVLTREAFGLVEDYIDRALGDDAVKGVVITSGKKDFAGGMDLNVLATIREESGEN
PAQGLFDFTMNGHRILRKLERAGMDAKNNKGGKPIACAINGTCAGIGTEIALACHYRTMTDNPKAKIGLPEILLGIFPGG
GGTIRYSRMVGAVNAAPVLLEGKMMDPKKAKSASLVDEVAADPLEAARQWVLNAKDADIVKPWDQKGYKMPGGAPYHPAG
FMNFVGASAMVHGKTQGAFPAAKALLSAVYEGALVDFDTALRIEARWFTHVLMNPSSSAMIRSLFLNKQALEKGAVRPKG
IEDQSVKKIGVLGAGMMGAGIALVSAQAGMEVVLIDRDQDAADKGKAYSATYMDKGIKRGKATPEKKEALLAQITATADL
DALKGCDLIIEAVFEDPGVKAEMTKKVEAIIPEDCIFASNTSTLPITSLAEASVRPEQFIGIHFFSPVEKMFLVEIIKGE
KTGDRAVAKALDYVRQIRKTPIVVNDARFFYCNRCIIPYINEGARMITEGVSPVLIDNAARQLGFPVGPIQLTDETSIDL
GAKIARATKAAMGDAYPESPADDLIFWMEDQGRLGRKANAGFFDYDDKGKRVEYWKGLAEKYPLAEVQPDLVEVQERLMF
AQVLEAVRALEENVLMDIREGDVGAVLAWGFAPWSGGPLSWLDIIGTPYAAERCDQLAEAYGDRFACPPLLREMAEKGQT
FYGRFAPEEDQAA

Sequences:

>Translated_733_residues
MTDFKYDVDADGIAIITWDAEGKSMNVLTREAFGLVEDYIDRALGDDAVKGVVITSGKKDFAGGMDLNVLATIREESGEN
PAQGLFDFTMNGHRILRKLERAGMDAKNNKGGKPIACAINGTCAGIGTEIALACHYRTMTDNPKAKIGLPEILLGIFPGG
GGTIRYSRMVGAVNAAPVLLEGKMMDPKKAKSASLVDEVAADPLEAARQWVLNAKDADIVKPWDQKGYKMPGGAPYHPAG
FMNFVGASAMVHGKTQGAFPAAKALLSAVYEGALVDFDTALRIEARWFTHVLMNPSSSAMIRSLFLNKQALEKGAVRPKG
IEDQSVKKIGVLGAGMMGAGIALVSAQAGMEVVLIDRDQDAADKGKAYSATYMDKGIKRGKATPEKKEALLAQITATADL
DALKGCDLIIEAVFEDPGVKAEMTKKVEAIIPEDCIFASNTSTLPITSLAEASVRPEQFIGIHFFSPVEKMFLVEIIKGE
KTGDRAVAKALDYVRQIRKTPIVVNDARFFYCNRCIIPYINEGARMITEGVSPVLIDNAARQLGFPVGPIQLTDETSIDL
GAKIARATKAAMGDAYPESPADDLIFWMEDQGRLGRKANAGFFDYDDKGKRVEYWKGLAEKYPLAEVQPDLVEVQERLMF
AQVLEAVRALEENVLMDIREGDVGAVLAWGFAPWSGGPLSWLDIIGTPYAAERCDQLAEAYGDRFACPPLLREMAEKGQT
FYGRFAPEEDQAA
>Mature_732_residues
TDFKYDVDADGIAIITWDAEGKSMNVLTREAFGLVEDYIDRALGDDAVKGVVITSGKKDFAGGMDLNVLATIREESGENP
AQGLFDFTMNGHRILRKLERAGMDAKNNKGGKPIACAINGTCAGIGTEIALACHYRTMTDNPKAKIGLPEILLGIFPGGG
GTIRYSRMVGAVNAAPVLLEGKMMDPKKAKSASLVDEVAADPLEAARQWVLNAKDADIVKPWDQKGYKMPGGAPYHPAGF
MNFVGASAMVHGKTQGAFPAAKALLSAVYEGALVDFDTALRIEARWFTHVLMNPSSSAMIRSLFLNKQALEKGAVRPKGI
EDQSVKKIGVLGAGMMGAGIALVSAQAGMEVVLIDRDQDAADKGKAYSATYMDKGIKRGKATPEKKEALLAQITATADLD
ALKGCDLIIEAVFEDPGVKAEMTKKVEAIIPEDCIFASNTSTLPITSLAEASVRPEQFIGIHFFSPVEKMFLVEIIKGEK
TGDRAVAKALDYVRQIRKTPIVVNDARFFYCNRCIIPYINEGARMITEGVSPVLIDNAARQLGFPVGPIQLTDETSIDLG
AKIARATKAAMGDAYPESPADDLIFWMEDQGRLGRKANAGFFDYDDKGKRVEYWKGLAEKYPLAEVQPDLVEVQERLMFA
QVLEAVRALEENVLMDIREGDVGAVLAWGFAPWSGGPLSWLDIIGTPYAAERCDQLAEAYGDRFACPPLLREMAEKGQTF
YGRFAPEEDQAA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=755, Percent_Identity=32.1854304635762, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI68989263, Length=720, Percent_Identity=24.7222222222222, Blast_Score=230, Evalue=3e-60,
Organism=Homo sapiens, GI261878539, Length=619, Percent_Identity=25.5250403877221, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI296179429, Length=295, Percent_Identity=33.8983050847458, Blast_Score=156, Evalue=8e-38,
Organism=Homo sapiens, GI296179427, Length=305, Percent_Identity=31.8032786885246, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788682, Length=729, Percent_Identity=36.4883401920439, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1790281, Length=740, Percent_Identity=32.5675675675676, Blast_Score=294, Evalue=2e-80,
Organism=Escherichia coli, GI1787661, Length=367, Percent_Identity=29.1553133514986, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1787659, Length=174, Percent_Identity=32.7586206896552, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=747, Percent_Identity=32.7978580990629, Blast_Score=335, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17508951, Length=742, Percent_Identity=32.6145552560647, Blast_Score=318, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17508953, Length=750, Percent_Identity=32.5333333333333, Blast_Score=318, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI25144276, Length=656, Percent_Identity=32.3170731707317, Blast_Score=277, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71985923, Length=372, Percent_Identity=30.3763440860215, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71985930, Length=368, Percent_Identity=28.804347826087, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17549919, Length=291, Percent_Identity=29.553264604811, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17553560, Length=295, Percent_Identity=30.8474576271186, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17563036, Length=292, Percent_Identity=30.1369863013699, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=177, Percent_Identity=29.3785310734463, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24583077, Length=746, Percent_Identity=32.9758713136729, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24583079, Length=746, Percent_Identity=32.9758713136729, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI19921000, Length=746, Percent_Identity=32.9758713136729, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI20129971, Length=204, Percent_Identity=29.9019607843137, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24653477, Length=204, Percent_Identity=29.9019607843137, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45549573, Length=214, Percent_Identity=27.5700934579439, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 79337; Mature: 79206

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFKYDVDADGIAIITWDAEGKSMNVLTREAFGLVEDYIDRALGDDAVKGVVITSGKKD
CCCCCEECCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCC
FAGGMDLNVLATIREESGENPAQGLFDFTMNGHRILRKLERAGMDAKNNKGGKPIACAIN
CCCCCCEEEEEEEEHHCCCCHHHCHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
GTCAGIGTEIALACHYRTMTDNPKAKIGLPEILLGIFPGGGGTIRYSRMVGAVNAAPVLL
CEEECCCCEEEEEEEEEECCCCCCCCCCCHHHHHEEECCCCCEEEEHHHHCCCCCCCEEE
EGKMMDPKKAKSASLVDEVAADPLEAARQWVLNAKDADIVKPWDQKGYKMPGGAPYHPAG
ECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
FMNFVGASAMVHGKTQGAFPAAKALLSAVYEGALVDFDTALRIEARWFTHVLMNPSSSAM
HHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCEEECCCEEEEEHEEEEHEEECCCHHHH
IRSLFLNKQALEKGAVRPKGIEDQSVKKIGVLGAGMMGAGIALVSAQAGMEVVLIDRDQD
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCCCEEECCCCCEEEEEECCCC
AADKGKAYSATYMDKGIKRGKATPEKKEALLAQITATADLDALKGCDLIIEAVFEDPGVK
CCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCCC
AEMTKKVEAIIPEDCIFASNTSTLPITSLAEASVRPEQFIGIHFFSPVEKMFLVEIIKGE
HHHHHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCHHHEEEEEEHHHHHHHHHHHHHCCC
KTGDRAVAKALDYVRQIRKTPIVVNDARFFYCNRCIIPYINEGARMITEGVSPVLIDNAA
CCCHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCEECCCHHHHHHCCCCEEECHHH
RQLGFPVGPIQLTDETSIDLGAKIARATKAAMGDAYPESPADDLIFWMEDQGRLGRKANA
HHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
GFFDYDDKGKRVEYWKGLAEKYPLAEVQPDLVEVQERLMFAQVLEAVRALEENVLMDIRE
CCCCCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
GDVGAVLAWGFAPWSGGPLSWLDIIGTPYAAERCDQLAEAYGDRFACPPLLREMAEKGQT
CCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCE
FYGRFAPEEDQAA
EEECCCCCCCCCC
>Mature Secondary Structure 
TDFKYDVDADGIAIITWDAEGKSMNVLTREAFGLVEDYIDRALGDDAVKGVVITSGKKD
CCCCEECCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCC
FAGGMDLNVLATIREESGENPAQGLFDFTMNGHRILRKLERAGMDAKNNKGGKPIACAIN
CCCCCCEEEEEEEEHHCCCCHHHCHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
GTCAGIGTEIALACHYRTMTDNPKAKIGLPEILLGIFPGGGGTIRYSRMVGAVNAAPVLL
CEEECCCCEEEEEEEEEECCCCCCCCCCCHHHHHEEECCCCCEEEEHHHHCCCCCCCEEE
EGKMMDPKKAKSASLVDEVAADPLEAARQWVLNAKDADIVKPWDQKGYKMPGGAPYHPAG
ECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
FMNFVGASAMVHGKTQGAFPAAKALLSAVYEGALVDFDTALRIEARWFTHVLMNPSSSAM
HHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCEEECCCEEEEEHEEEEHEEECCCHHHH
IRSLFLNKQALEKGAVRPKGIEDQSVKKIGVLGAGMMGAGIALVSAQAGMEVVLIDRDQD
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCCCEEECCCCCEEEEEECCCC
AADKGKAYSATYMDKGIKRGKATPEKKEALLAQITATADLDALKGCDLIIEAVFEDPGVK
CCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCCC
AEMTKKVEAIIPEDCIFASNTSTLPITSLAEASVRPEQFIGIHFFSPVEKMFLVEIIKGE
HHHHHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCHHHEEEEEEHHHHHHHHHHHHHCCC
KTGDRAVAKALDYVRQIRKTPIVVNDARFFYCNRCIIPYINEGARMITEGVSPVLIDNAA
CCCHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCEECCCHHHHHHCCCCEEECHHH
RQLGFPVGPIQLTDETSIDLGAKIARATKAAMGDAYPESPADDLIFWMEDQGRLGRKANA
HHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
GFFDYDDKGKRVEYWKGLAEKYPLAEVQPDLVEVQERLMFAQVLEAVRALEENVLMDIRE
CCCCCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
GDVGAVLAWGFAPWSGGPLSWLDIIGTPYAAERCDQLAEAYGDRFACPPLLREMAEKGQT
CCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCE
FYGRFAPEEDQAA
EEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA