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The map label for this gene is purN [H]

Identifier: 99081316

GI number: 99081316

Start: 1575595

End: 1576149

Strand: Reverse

Name: purN [H]

Synonym: TM1040_1475

Alternate gene names: 99081316

Gene position: 1576149-1575595 (Counterclockwise)

Preceding gene: 99081317

Following gene: 99081315

Centisome position: 49.24

GC content: 64.14

Gene sequence:

>555_bases
ATGGTGTCCCTCGTCGACAGCATGTTGAACGACGCCGATCATCCGGGTTCGCCTTGCCTGGTGCTGTCCAACAACGCGGA
TGCCGGGGGCCTCTCCAAGGCCGCCGAACGCGGGGTCGCCACGGCGGTGGTCGATCACCGCCCCTTTGGCAAGGATCGCG
CAGCATTTGAGGCTGAGCTGGTGCAACCCATTCTGGAGGCTGGCGCTGATGTGGTCTGTCTTGCGGGCTTCATGCGGGTG
TTGACCGCAGGTTTCGTGCGCCAGTTCGAAGGCCGGATGCTCAATATCCATCCCTCCCTGCTCCCCAAATACAAGGGCTT
GCACACCCATGCCCGCGCTCTGGAAGCTGGCGACCTGCGCCATGGCTGCAGTGTGCATGAGGTGACTCCGCTCCTCGATG
ACGGCCCGATTCTGGGGCAGGCCGAAGTGCCCGTTCATCCCGGCGACACGCCCGATGATCTGGCCGCGCGGGTGCTCGTT
CAGGAACATCGCCTCTATCCGGCGGTGCTGGAGCGCTATCTGCGTGGCGAGCGTGCAGTCTTGCACCTGAGCTGA

Upstream 100 bases:

>100_bases
TTGGCACTGTGACGCGCGGGGAGGGTATTCGCTACACGGGCGCGCTGCTGTGAGCGACAAGAAACGCGTTGCGATCCTGA
TTTCCGGCGGGGGCTCCAAT

Downstream 100 bases:

>100_bases
GCCGAAGCCTTTAGGGGGTTTGCAACCGCCTCTTGGTCGTCGTATCAACGGGAAACCGAGCGGCAGAAACCCTTAAAAAA
GACCCAGTTGATGAAAACTC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MVSLVDSMLNDADHPGSPCLVLSNNADAGGLSKAAERGVATAVVDHRPFGKDRAAFEAELVQPILEAGADVVCLAGFMRV
LTAGFVRQFEGRMLNIHPSLLPKYKGLHTHARALEAGDLRHGCSVHEVTPLLDDGPILGQAEVPVHPGDTPDDLAARVLV
QEHRLYPAVLERYLRGERAVLHLS

Sequences:

>Translated_184_residues
MVSLVDSMLNDADHPGSPCLVLSNNADAGGLSKAAERGVATAVVDHRPFGKDRAAFEAELVQPILEAGADVVCLAGFMRV
LTAGFVRQFEGRMLNIHPSLLPKYKGLHTHARALEAGDLRHGCSVHEVTPLLDDGPILGQAEVPVHPGDTPDDLAARVLV
QEHRLYPAVLERYLRGERAVLHLS
>Mature_184_residues
MVSLVDSMLNDADHPGSPCLVLSNNADAGGLSKAAERGVATAVVDHRPFGKDRAAFEAELVQPILEAGADVVCLAGFMRV
LTAGFVRQFEGRMLNIHPSLLPKYKGLHTHARALEAGDLRHGCSVHEVTPLLDDGPILGQAEVPVHPGDTPDDLAARVLV
QEHRLYPAVLERYLRGERAVLHLS

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=41.2429378531073, Blast_Score=140, Evalue=7e-34,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=41.2429378531073, Blast_Score=140, Evalue=7e-34,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=41.2429378531073, Blast_Score=140, Evalue=7e-34,
Organism=Escherichia coli, GI1788846, Length=161, Percent_Identity=52.1739130434783, Blast_Score=160, Evalue=3e-41,
Organism=Escherichia coli, GI1787483, Length=138, Percent_Identity=32.6086956521739, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17567511, Length=169, Percent_Identity=39.0532544378698, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24582400, Length=157, Percent_Identity=45.859872611465, Blast_Score=144, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 19774; Mature: 19774

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLVDSMLNDADHPGSPCLVLSNNADAGGLSKAAERGVATAVVDHRPFGKDRAAFEAEL
CHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
VQPILEAGADVVCLAGFMRVLTAGFVRQFEGRMLNIHPSLLPKYKGLHTHARALEAGDLR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCCHHHHHHHHHHCCCC
HGCSVHEVTPLLDDGPILGQAEVPVHPGDTPDDLAARVLVQEHRLYPAVLERYLRGERAV
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
LHLS
EECC
>Mature Secondary Structure
MVSLVDSMLNDADHPGSPCLVLSNNADAGGLSKAAERGVATAVVDHRPFGKDRAAFEAEL
CHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
VQPILEAGADVVCLAGFMRVLTAGFVRQFEGRMLNIHPSLLPKYKGLHTHARALEAGDLR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCCHHHHHHHHHHCCCC
HGCSVHEVTPLLDDGPILGQAEVPVHPGDTPDDLAARVLVQEHRLYPAVLERYLRGERAV
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
LHLS
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]