Click here to switch to the map view.

The map label for this gene is metH [H]

Identifier: 99081309

GI number: 99081309

Start: 1569216

End: 1570238

Strand: Direct

Name: metH [H]

Synonym: TM1040_1468

Alternate gene names: 99081309

Gene position: 1569216-1570238 (Clockwise)

Preceding gene: 99081308

Following gene: 99081311

Centisome position: 49.02

GC content: 62.95

Gene sequence:

>1023_bases
ATGAGCAACAGTTTCCAAGAGCTTCTGGACAGCCGTGATGTGCTTCTGGCGGATGGCGCGACAGGCACCAACCTGTTCAA
CATGGGGCTGCAGTCCGGTGATGCGCCGGAACTGTGGAACACCGACGCGCCAGACAAGATCAAGGCACTCTATCAAGGCT
CGGTCGATGCCGGCAGCGATCTGTTTTTGACCAATAGCTTTGGTGGCACCGCCGCACGTCTGAAGCTGCATGACGCACAA
GGCCGTGTCCGCGAGCTGAACCGCATCGCCGCAGAACTTGGACGCGAAGTTGCGGACAAGGCTGAACGCAAGATTGCCGT
TGCCGGCTCTGTCGGACCCACCGGCGAAATCATGCAACCGGTTGGGGAACTCTCGCACGCGCTCGCGGTGGAAATGTTCC
ACGAACAGGCCGACGCGCTCAAGGAAGGCGGCGTAGACGTGCTCTGGCTCGAAACGATTTCCGCTCCCGAGGAATTTGCC
GCCGCCGCTGAGGCCTTCAAACTGGTGGATATGCCCTGGTGCGGCACCATGAGCTTTGACACCGCCGGACGTACCATGAT
GGGCGTGACCTCTGCCGATCTGGCCAAACTCGTCGAGGAGTTCGACACCCCGCCGCTCGCTTTTGGCGCCAACTGCGGCA
CCGGGGCATCGGATATCCTGCGCACCGTGCTCGGATTCGCCGCGCAGGGCACCGAACGTCCGATCATCTCCAAGGGCAAC
GCGGGTATTCCCAAATATGTCGATGGCCATATCCACTATGACGGCACCCCGGACTTGATGGGCGAATACGCCGTCCTGGC
GCGCGACTCCGGAGCCAAGATCATCGGCGGCTGCTGCGGCACCATGCCGGACCATCTGCGCAAGATGCGTGAAGCCCTCG
ACAGCCGCCCGCGCGGCGAACGCCCGACGCTGGAACATATCGTAGAGCTGCTTGGCCCGTTCAGCTCGGCCAACGACGGC
ACCGAAGAAGGCAGCGATGCCAGCGCTGAGCGCCGAGGCCGTCGCGGGCGCCGTCGCAGCTGA

Upstream 100 bases:

>100_bases
CTGTCGAAAATGCAGTTGCGGCAAAACGGACCGCGTGACAAAGAAAACGCCTTGCGGAGCCAAAGCGCTCTGTGTGCGAA
TTGGGTAAGTGGACAAGAAC

Downstream 100 bases:

>100_bases
TCCGATCCTCAGCATGCGTCCTTGAAAGAGCCGGTCATTGAACAGGCTCTTTATTTATGCGCTGTCAGAATTAAGCGGTG
TCAGGAAAACAGGCTGAGCT

Product: methionine synthase I

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSNSFQELLDSRDVLLADGATGTNLFNMGLQSGDAPELWNTDAPDKIKALYQGSVDAGSDLFLTNSFGGTAARLKLHDAQ
GRVRELNRIAAELGREVADKAERKIAVAGSVGPTGEIMQPVGELSHALAVEMFHEQADALKEGGVDVLWLETISAPEEFA
AAAEAFKLVDMPWCGTMSFDTAGRTMMGVTSADLAKLVEEFDTPPLAFGANCGTGASDILRTVLGFAAQGTERPIISKGN
AGIPKYVDGHIHYDGTPDLMGEYAVLARDSGAKIIGGCCGTMPDHLRKMREALDSRPRGERPTLEHIVELLGPFSSANDG
TEEGSDASAERRGRRGRRRS

Sequences:

>Translated_340_residues
MSNSFQELLDSRDVLLADGATGTNLFNMGLQSGDAPELWNTDAPDKIKALYQGSVDAGSDLFLTNSFGGTAARLKLHDAQ
GRVRELNRIAAELGREVADKAERKIAVAGSVGPTGEIMQPVGELSHALAVEMFHEQADALKEGGVDVLWLETISAPEEFA
AAAEAFKLVDMPWCGTMSFDTAGRTMMGVTSADLAKLVEEFDTPPLAFGANCGTGASDILRTVLGFAAQGTERPIISKGN
AGIPKYVDGHIHYDGTPDLMGEYAVLARDSGAKIIGGCCGTMPDHLRKMREALDSRPRGERPTLEHIVELLGPFSSANDG
TEEGSDASAERRGRRGRRRS
>Mature_339_residues
SNSFQELLDSRDVLLADGATGTNLFNMGLQSGDAPELWNTDAPDKIKALYQGSVDAGSDLFLTNSFGGTAARLKLHDAQG
RVRELNRIAAELGREVADKAERKIAVAGSVGPTGEIMQPVGELSHALAVEMFHEQADALKEGGVDVLWLETISAPEEFAA
AAEAFKLVDMPWCGTMSFDTAGRTMMGVTSADLAKLVEEFDTPPLAFGANCGTGASDILRTVLGFAAQGTERPIISKGNA
GIPKYVDGHIHYDGTPDLMGEYAVLARDSGAKIIGGCCGTMPDHLRKMREALDSRPRGERPTLEHIVELLGPFSSANDGT
EEGSDASAERRGRRGRRRS

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=283, Percent_Identity=28.9752650176678, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI13162290, Length=308, Percent_Identity=26.9480519480519, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790450, Length=334, Percent_Identity=27.8443113772455, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786456, Length=318, Percent_Identity=27.0440251572327, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535405, Length=331, Percent_Identity=31.4199395770393, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 36223; Mature: 36092

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSFQELLDSRDVLLADGATGTNLFNMGLQSGDAPELWNTDAPDKIKALYQGSVDAGSD
CCCHHHHHHCCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
LFLTNSFGGTAARLKLHDAQGRVRELNRIAAELGREVADKAERKIAVAGSVGPTGEIMQP
EEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHH
VGELSHALAVEMFHEQADALKEGGVDVLWLETISAPEEFAAAAEAFKLVDMPWCGTMSFD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TAGRTMMGVTSADLAKLVEEFDTPPLAFGANCGTGASDILRTVLGFAAQGTERPIISKGN
CCCCEEECCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
AGIPKYVDGHIHYDGTPDLMGEYAVLARDSGAKIIGGCCGTMPDHLRKMREALDSRPRGE
CCCCCCCCCEEEECCCHHHHHCCCEEEECCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCC
RPTLEHIVELLGPFSSANDGTEEGSDASAERRGRRGRRRS
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SNSFQELLDSRDVLLADGATGTNLFNMGLQSGDAPELWNTDAPDKIKALYQGSVDAGSD
CCHHHHHHCCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
LFLTNSFGGTAARLKLHDAQGRVRELNRIAAELGREVADKAERKIAVAGSVGPTGEIMQP
EEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHH
VGELSHALAVEMFHEQADALKEGGVDVLWLETISAPEEFAAAAEAFKLVDMPWCGTMSFD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TAGRTMMGVTSADLAKLVEEFDTPPLAFGANCGTGASDILRTVLGFAAQGTERPIISKGN
CCCCEEECCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
AGIPKYVDGHIHYDGTPDLMGEYAVLARDSGAKIIGGCCGTMPDHLRKMREALDSRPRGE
CCCCCCCCCEEEECCCHHHHHCCCEEEECCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCC
RPTLEHIVELLGPFSSANDGTEEGSDASAERRGRRGRRRS
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]