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The map label for this gene is mtbC1 [H]

Identifier: 99081311

GI number: 99081311

Start: 1571618

End: 1572316

Strand: Direct

Name: mtbC1 [H]

Synonym: TM1040_1470

Alternate gene names: 99081311

Gene position: 1571618-1572316 (Clockwise)

Preceding gene: 99081309

Following gene: 99081312

Centisome position: 49.1

GC content: 58.66

Gene sequence:

>699_bases
ATGTCGGAAGAAGAAGACATCATCCTCTCAGAACTCGATGACGAGGAACTGGTCCAACAAATGTTTGACGACCTCTATGA
CGGTCTGAAGGAAGAAATCGAGGAAGGCGTCAACATCCTGCTCGAACGCGGCTGGGCGCCCTACAAGATCCTGACCGAAG
CACTGGTCGGCGGCATGACCATCGTGGGTGCAGACTTCCGCGACGGCATTCTCTTCGTTCCCGAAGTGCTTCTGGCGGCC
AACGCCATGAAGGGTGGCATGACGATCCTGAAGCCGCTTCTGGCAGAAACCGGCGCACCGCGCATGGGCTCCATGGTGAT
CGGCACCGTGAAAGGCGACATTCACGACATCGGCAAGAACCTCGTCTCGATGATGATGGAAGGCGCCGGTTTTGAGGTGG
TGGACATCGGCATCAACAACCCGGTCGAAAACTACCTCGAGGCCCTCGAAGAGCACAAACCCGACATCCTCGGCATGTCC
GCGCTCCTGACCACCACCATGCCCTACATGAAGGTCGTAATCGACACGATGGTCGAGCAAGGCAAGCGTGACGATTACAT
CGTGCTGGTGGGCGGCGCGCCCCTCAACGAAGAATTCGGCAAGGCCATCGGCGCCGACGCCTACTGCCGCGACGCGGCTG
TCGCGGTTGAAACCGCCAAAGAATTTGTGGCGCGCAAGCACAACGCCATGAACGCCTGA

Upstream 100 bases:

>100_bases
ACGCGCCAATGTCGCAATCAAAACAGTGGAGTTCTGAACATCTGTCAAAAACACATAAAAGCGCCAATTCAGGCCCGCCC
GCCAGCTAAGGAAGACAGAT

Downstream 100 bases:

>100_bases
TATGGCCACCGCGTCCCCTCGACGCGGTCGGGCGGCCCGACTTTCAAAGCGGGCCGCTCTGTCTTTGCCACAGGGCAAAT
CTCTCGACGCACAAAGCCTG

Product: 5-methyltetrahydrofolate--homocysteine S-methyltransferase

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSEEEDIILSELDDEELVQQMFDDLYDGLKEEIEEGVNILLERGWAPYKILTEALVGGMTIVGADFRDGILFVPEVLLAA
NAMKGGMTILKPLLAETGAPRMGSMVIGTVKGDIHDIGKNLVSMMMEGAGFEVVDIGINNPVENYLEALEEHKPDILGMS
ALLTTTMPYMKVVIDTMVEQGKRDDYIVLVGGAPLNEEFGKAIGADAYCRDAAVAVETAKEFVARKHNAMNA

Sequences:

>Translated_232_residues
MSEEEDIILSELDDEELVQQMFDDLYDGLKEEIEEGVNILLERGWAPYKILTEALVGGMTIVGADFRDGILFVPEVLLAA
NAMKGGMTILKPLLAETGAPRMGSMVIGTVKGDIHDIGKNLVSMMMEGAGFEVVDIGINNPVENYLEALEEHKPDILGMS
ALLTTTMPYMKVVIDTMVEQGKRDDYIVLVGGAPLNEEFGKAIGADAYCRDAAVAVETAKEFVARKHNAMNA
>Mature_231_residues
SEEEDIILSELDDEELVQQMFDDLYDGLKEEIEEGVNILLERGWAPYKILTEALVGGMTIVGADFRDGILFVPEVLLAAN
AMKGGMTILKPLLAETGAPRMGSMVIGTVKGDIHDIGKNLVSMMMEGAGFEVVDIGINNPVENYLEALEEHKPDILGMSA
LLTTTMPYMKVVIDTMVEQGKRDDYIVLVGGAPLNEEFGKAIGADAYCRDAAVAVETAKEFVARKHNAMNA

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=160, Percent_Identity=35.625, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1790450, Length=186, Percent_Identity=32.7956989247312, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17535405, Length=160, Percent_Identity=37.5, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 25228; Mature: 25097

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEEDIILSELDDEELVQQMFDDLYDGLKEEIEEGVNILLERGWAPYKILTEALVGGMT
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEE
IVGADFRDGILFVPEVLLAANAMKGGMTILKPLLAETGAPRMGSMVIGTVKGDIHDIGKN
EECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHH
LVSMMMEGAGFEVVDIGINNPVENYLEALEEHKPDILGMSALLTTTMPYMKVVIDTMVEQ
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GKRDDYIVLVGGAPLNEEFGKAIGADAYCRDAAVAVETAKEFVARKHNAMNA
CCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEEEDIILSELDDEELVQQMFDDLYDGLKEEIEEGVNILLERGWAPYKILTEALVGGMT
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEE
IVGADFRDGILFVPEVLLAANAMKGGMTILKPLLAETGAPRMGSMVIGTVKGDIHDIGKN
EECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHH
LVSMMMEGAGFEVVDIGINNPVENYLEALEEHKPDILGMSALLTTTMPYMKVVIDTMVEQ
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GKRDDYIVLVGGAPLNEEFGKAIGADAYCRDAAVAVETAKEFVARKHNAMNA
CCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]