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The map label for this gene is kefA [C]

Identifier: 99081279

GI number: 99081279

Start: 1534364

End: 1536886

Strand: Reverse

Name: kefA [C]

Synonym: TM1040_1438

Alternate gene names: 99081279

Gene position: 1536886-1534364 (Counterclockwise)

Preceding gene: 99081280

Following gene: 99081278

Centisome position: 48.01

GC content: 60.29

Gene sequence:

>2523_bases
ATGACTTATTTGAGAACGCTTTTTCAGGTCGGCCTTTTGGGGCTGACCTGTTTGCTTTTGCCTATTGTTGCTTTGGCGCA
GGAAACCGGCGGGCAGACCCGGCTGCACGAGGCCCGCGATCTGGTGCAGACCGCCGCTTCTGGCAAATTGGGCGAGTATT
ATGAGGCCTGGCAGCGCACTGCAGATCGTGCAGAGCGGGTGCTGGATGCCGGACGCGCGTCCAATGGCGCGCTGGAGGCC
CTGCGGCTGGAGCTGACGGAGTATCGTCAGCAATTTCTCGACGCAGGCAGCCAGAATGCGGATCGCCTGAGAACTCTTCG
CGCACAGATCGAGGCACTCGGGCCACCGCCCGAAGAAAATGGCAGCGAGCCTGAAAACATTGCCAAACTGCGGCAGGAGT
TGCAGAGCCAGCTTGCCAACCTCAGCGCGCCGCAGGTGCTGGCGGATCTCGGCTATAGCCGGGCGCAGGGTCTGATTACC
GAAATCGACAAGCTGATCCGAGAACGCGCAACCCGCACGCTCTTGGAGCGCGCGCCAATGCCGGTCAATCCCGAGCACTG
GCCGACCGCCTTCTCTGACACGGCAAAGGTGCTGGGCGCGTTCTGGTACGAGACCGCAACCCTTCTGTCGAGCGCGGCCA
CGCTTGAAACCATCCGGGGCCGTTTGCCGGTCCTGCTGCTGTTTACTGCCGGGGCGCTGCTGCTCTTGGGGCGCGGACGC
TACTGGGCGCGTCGTGCGGGCACCCATCTGCGCGAACTCGGCTGGCGCGGCACAGGCGTCTGGCGGTTCATCGTCTCGCT
TCTGCAGGTGCTGTTGCCCTATGTGGGGACGGTTCTTCTGGTGCGTGCGATCGATATGTCCGGTGTGCTGGGCGTCAGGG
GGACATTGCTGTTGGATGATGTGCCGCTGTGGTCCTTTATCCTGTTTGCTTCGCATTGGCTGGGCGAGCGGCTTTATGTG
TATCAGATCACCAATGAGCTCATACCCTTTGAGCCGCGCTTTCAGCGTCAGGTGCGTGTGATCCTTGTGTCGCTGGCACT
TGTGCTGGTGATGCAGATGATGCTGGATCGTCTCGACACCATCGAGAATTTCACCGATGCGACCTATGCGGTATTGAGCT
GGCCTCTGGTCATTCTCGCTTCGCTGTTCCTGGTACGTCTGAACCGTATCCGGGTGCGCCAGCGCCGCCTCGAGATCGAA
GAAAACGGCGGCAGCGAGGATGAGGGCGCCATTGCGGCCGGGGCCAATCGCGCCATGTCGCTTCTGCGGCGGGCGGTGTT
CATCATGGCCCTTGTTGCGCCGATCCTTGGGGCTCTGGGCTATATTAACGCAGCAATCGCAATTGTTTACCCGGTGATCC
TGACGCTTGGTCTGGTCACCACCCTGATGATCCTGCAGCGGTTCCTGTCGGATATATACGGATATGTCACGCGCAACGGT
CAAGGCGCGGAGGACTCGCTCTTTGCAGTGATCACCGGCTTTGTACTGGCCCTGCTGTCGCTGCCCTTCTTTGCGCTGAT
CTGGGGCGCGCGGACCTCGGATTTGACCGAACTCTGGTCGCGCTTCCTCGAAGGCTACAGCATCGGCGACACCAAGATCT
CTCCGACGATCTTCCTGACGTTTGTGGTGATTTTTGTCTGCGGTTATGTGCTGACCCGGCTCTTGCAGGGGAGTCTGCGC
TCGTCACTGCTGCCAAAGACCAAGATTGACCCCGGTGGGCAGAACGCGATTGTGTCCGGTGTCGGCTATATCGGCATCTT
CCTGTCGGCGCTCATCGCGGTTTCTACCGCCGGGCTCGACCTGTCGTCGCTCGCGATTGTTGCCGGTGCGCTCTCGGTCG
GGATCGGCTTTGGCCTGCAGACGATCGTGTCGAACTTCGTGTCGGGCATCATCCTGTTGATCGAACGACCGGTCTCCAAG
GGAGATTGGATCGAGGTAGGCGGGCTCATGGGCTATGTGCGTGACATCTCTGTGCGCTCCACCCGGATCGAGACGTTTGA
CCGTACCGATGTGATTGTCCCGAACTCGGATCTGATCTCCGGCACCGTCACGAACTATACCCGAGGCAATACTGTTGGAC
GGGTGATCGTTCCGGTTGGCGTGGCCTATGGTACCGATCCGCGCAAGGTCGAGGAGATCCTCATGGAGGTTGCGCAATCG
CATCCTATGGTCCTGATGAACCCGGCACCTTCGGTCGTGTTCCAGGGCTTTGGGGCGGATAGTCTCGACTTTGAGATCCG
CGCCATCCTGCGCGATGTGAACTGGGTTCTGTCGGTGAAGTCCGACATGAACTACGAGATCGCCAAGAGATTTACGGAAG
AAGGGATCGAGATTCCCTTTGCCCAGCGTGACATCTGGATCCGCAATCCCGAGGCGATCCAGACCAGCAAAGAGTCACCT
CAGGCCGAGACCTCGACTTCAGCAGGCGCATCAACGCATGCGGCTCGGCTCAAACCCGATCTCGACGATCTTGCGCAGGA
TCAGGACGCCGACGCAGAGGGCGATGGCGACGGCGACCGCTAG

Upstream 100 bases:

>100_bases
TTAATCCGGAATTCCTCAAAGACAAGGGCTTGCCGGTCCCCGACTGGATGACCCATACTCCTGCTTCAATTCCCGGAGTA
TTCGAGGACATCTGAATCAG

Downstream 100 bases:

>100_bases
GTCTGAGCTCGCCTGCAAACTGATGCCACCAGAGTGCGCCGCTGCCGGGAGATCCCGCAGCGGCGCTTTCTTTTGTGGCG
CCGAGGGCAGGGGGGCGGAC

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 840; Mature: 839

Protein sequence:

>840_residues
MTYLRTLFQVGLLGLTCLLLPIVALAQETGGQTRLHEARDLVQTAASGKLGEYYEAWQRTADRAERVLDAGRASNGALEA
LRLELTEYRQQFLDAGSQNADRLRTLRAQIEALGPPPEENGSEPENIAKLRQELQSQLANLSAPQVLADLGYSRAQGLIT
EIDKLIRERATRTLLERAPMPVNPEHWPTAFSDTAKVLGAFWYETATLLSSAATLETIRGRLPVLLLFTAGALLLLGRGR
YWARRAGTHLRELGWRGTGVWRFIVSLLQVLLPYVGTVLLVRAIDMSGVLGVRGTLLLDDVPLWSFILFASHWLGERLYV
YQITNELIPFEPRFQRQVRVILVSLALVLVMQMMLDRLDTIENFTDATYAVLSWPLVILASLFLVRLNRIRVRQRRLEIE
ENGGSEDEGAIAAGANRAMSLLRRAVFIMALVAPILGALGYINAAIAIVYPVILTLGLVTTLMILQRFLSDIYGYVTRNG
QGAEDSLFAVITGFVLALLSLPFFALIWGARTSDLTELWSRFLEGYSIGDTKISPTIFLTFVVIFVCGYVLTRLLQGSLR
SSLLPKTKIDPGGQNAIVSGVGYIGIFLSALIAVSTAGLDLSSLAIVAGALSVGIGFGLQTIVSNFVSGIILLIERPVSK
GDWIEVGGLMGYVRDISVRSTRIETFDRTDVIVPNSDLISGTVTNYTRGNTVGRVIVPVGVAYGTDPRKVEEILMEVAQS
HPMVLMNPAPSVVFQGFGADSLDFEIRAILRDVNWVLSVKSDMNYEIAKRFTEEGIEIPFAQRDIWIRNPEAIQTSKESP
QAETSTSAGASTHAARLKPDLDDLAQDQDADAEGDGDGDR

Sequences:

>Translated_840_residues
MTYLRTLFQVGLLGLTCLLLPIVALAQETGGQTRLHEARDLVQTAASGKLGEYYEAWQRTADRAERVLDAGRASNGALEA
LRLELTEYRQQFLDAGSQNADRLRTLRAQIEALGPPPEENGSEPENIAKLRQELQSQLANLSAPQVLADLGYSRAQGLIT
EIDKLIRERATRTLLERAPMPVNPEHWPTAFSDTAKVLGAFWYETATLLSSAATLETIRGRLPVLLLFTAGALLLLGRGR
YWARRAGTHLRELGWRGTGVWRFIVSLLQVLLPYVGTVLLVRAIDMSGVLGVRGTLLLDDVPLWSFILFASHWLGERLYV
YQITNELIPFEPRFQRQVRVILVSLALVLVMQMMLDRLDTIENFTDATYAVLSWPLVILASLFLVRLNRIRVRQRRLEIE
ENGGSEDEGAIAAGANRAMSLLRRAVFIMALVAPILGALGYINAAIAIVYPVILTLGLVTTLMILQRFLSDIYGYVTRNG
QGAEDSLFAVITGFVLALLSLPFFALIWGARTSDLTELWSRFLEGYSIGDTKISPTIFLTFVVIFVCGYVLTRLLQGSLR
SSLLPKTKIDPGGQNAIVSGVGYIGIFLSALIAVSTAGLDLSSLAIVAGALSVGIGFGLQTIVSNFVSGIILLIERPVSK
GDWIEVGGLMGYVRDISVRSTRIETFDRTDVIVPNSDLISGTVTNYTRGNTVGRVIVPVGVAYGTDPRKVEEILMEVAQS
HPMVLMNPAPSVVFQGFGADSLDFEIRAILRDVNWVLSVKSDMNYEIAKRFTEEGIEIPFAQRDIWIRNPEAIQTSKESP
QAETSTSAGASTHAARLKPDLDDLAQDQDADAEGDGDGDR
>Mature_839_residues
TYLRTLFQVGLLGLTCLLLPIVALAQETGGQTRLHEARDLVQTAASGKLGEYYEAWQRTADRAERVLDAGRASNGALEAL
RLELTEYRQQFLDAGSQNADRLRTLRAQIEALGPPPEENGSEPENIAKLRQELQSQLANLSAPQVLADLGYSRAQGLITE
IDKLIRERATRTLLERAPMPVNPEHWPTAFSDTAKVLGAFWYETATLLSSAATLETIRGRLPVLLLFTAGALLLLGRGRY
WARRAGTHLRELGWRGTGVWRFIVSLLQVLLPYVGTVLLVRAIDMSGVLGVRGTLLLDDVPLWSFILFASHWLGERLYVY
QITNELIPFEPRFQRQVRVILVSLALVLVMQMMLDRLDTIENFTDATYAVLSWPLVILASLFLVRLNRIRVRQRRLEIEE
NGGSEDEGAIAAGANRAMSLLRRAVFIMALVAPILGALGYINAAIAIVYPVILTLGLVTTLMILQRFLSDIYGYVTRNGQ
GAEDSLFAVITGFVLALLSLPFFALIWGARTSDLTELWSRFLEGYSIGDTKISPTIFLTFVVIFVCGYVLTRLLQGSLRS
SLLPKTKIDPGGQNAIVSGVGYIGIFLSALIAVSTAGLDLSSLAIVAGALSVGIGFGLQTIVSNFVSGIILLIERPVSKG
DWIEVGGLMGYVRDISVRSTRIETFDRTDVIVPNSDLISGTVTNYTRGNTVGRVIVPVGVAYGTDPRKVEEILMEVAQSH
PMVLMNPAPSVVFQGFGADSLDFEIRAILRDVNWVLSVKSDMNYEIAKRFTEEGIEIPFAQRDIWIRNPEAIQTSKESPQ
AETSTSAGASTHAARLKPDLDDLAQDQDADAEGDGDGDR

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=250, Percent_Identity=36, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI2367355, Length=317, Percent_Identity=29.3375394321767, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI1789291, Length=247, Percent_Identity=28.3400809716599, Blast_Score=109, Evalue=8e-25,
Organism=Escherichia coli, GI1787591, Length=199, Percent_Identity=30.6532663316583, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 92207; Mature: 92076

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLRTLFQVGLLGLTCLLLPIVALAQETGGQTRLHEARDLVQTAASGKLGEYYEAWQRT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ADRAERVLDAGRASNGALEALRLELTEYRQQFLDAGSQNADRLRTLRAQIEALGPPPEEN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
GSEPENIAKLRQELQSQLANLSAPQVLADLGYSRAQGLITEIDKLIRERATRTLLERAPM
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVNPEHWPTAFSDTAKVLGAFWYETATLLSSAATLETIRGRLPVLLLFTAGALLLLGRGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
YWARRAGTHLRELGWRGTGVWRFIVSLLQVLLPYVGTVLLVRAIDMSGVLGVRGTLLLDD
HHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
VPLWSFILFASHWLGERLYVYQITNELIPFEPRFQRQVRVILVSLALVLVMQMMLDRLDT
CHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IENFTDATYAVLSWPLVILASLFLVRLNRIRVRQRRLEIEENGGSEDEGAIAAGANRAMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCHHHHHH
LLRRAVFIMALVAPILGALGYINAAIAIVYPVILTLGLVTTLMILQRFLSDIYGYVTRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGAEDSLFAVITGFVLALLSLPFFALIWGARTSDLTELWSRFLEGYSIGDTKISPTIFLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
FVVIFVCGYVLTRLLQGSLRSSLLPKTKIDPGGQNAIVSGVGYIGIFLSALIAVSTAGLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LSSLAIVAGALSVGIGFGLQTIVSNFVSGIILLIERPVSKGDWIEVGGLMGYVRDISVRS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHH
TRIETFDRTDVIVPNSDLISGTVTNYTRGNTVGRVIVPVGVAYGTDPRKVEEILMEVAQS
HHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCHHHHHHHHHHHHCC
HPMVLMNPAPSVVFQGFGADSLDFEIRAILRDVNWVLSVKSDMNYEIAKRFTEEGIEIPF
CCEEEECCCCHHHEECCCCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCCCC
AQRDIWIRNPEAIQTSKESPQAETSTSAGASTHAARLKPDLDDLAQDQDADAEGDGDGDR
CCCCCEECCCHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TYLRTLFQVGLLGLTCLLLPIVALAQETGGQTRLHEARDLVQTAASGKLGEYYEAWQRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ADRAERVLDAGRASNGALEALRLELTEYRQQFLDAGSQNADRLRTLRAQIEALGPPPEEN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
GSEPENIAKLRQELQSQLANLSAPQVLADLGYSRAQGLITEIDKLIRERATRTLLERAPM
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVNPEHWPTAFSDTAKVLGAFWYETATLLSSAATLETIRGRLPVLLLFTAGALLLLGRGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
YWARRAGTHLRELGWRGTGVWRFIVSLLQVLLPYVGTVLLVRAIDMSGVLGVRGTLLLDD
HHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
VPLWSFILFASHWLGERLYVYQITNELIPFEPRFQRQVRVILVSLALVLVMQMMLDRLDT
CHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IENFTDATYAVLSWPLVILASLFLVRLNRIRVRQRRLEIEENGGSEDEGAIAAGANRAMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCHHHHHH
LLRRAVFIMALVAPILGALGYINAAIAIVYPVILTLGLVTTLMILQRFLSDIYGYVTRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGAEDSLFAVITGFVLALLSLPFFALIWGARTSDLTELWSRFLEGYSIGDTKISPTIFLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
FVVIFVCGYVLTRLLQGSLRSSLLPKTKIDPGGQNAIVSGVGYIGIFLSALIAVSTAGLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LSSLAIVAGALSVGIGFGLQTIVSNFVSGIILLIERPVSKGDWIEVGGLMGYVRDISVRS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHH
TRIETFDRTDVIVPNSDLISGTVTNYTRGNTVGRVIVPVGVAYGTDPRKVEEILMEVAQS
HHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCHHHHHHHHHHHHCC
HPMVLMNPAPSVVFQGFGADSLDFEIRAILRDVNWVLSVKSDMNYEIAKRFTEEGIEIPF
CCEEEECCCCHHHEECCCCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCCCC
AQRDIWIRNPEAIQTSKESPQAETSTSAGASTHAARLKPDLDDLAQDQDADAEGDGDGDR
CCCCCEECCCHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]