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The map label for this gene is cbs [H]

Identifier: 99081280

GI number: 99081280

Start: 1536892

End: 1538076

Strand: Reverse

Name: cbs [H]

Synonym: TM1040_1439

Alternate gene names: 99081280

Gene position: 1538076-1536892 (Counterclockwise)

Preceding gene: 99081281

Following gene: 99081279

Centisome position: 48.05

GC content: 59.92

Gene sequence:

>1185_bases
ATGGTTTTGCGGGCAAGTCAGACGCAGTGGAACCACGGTCGCGCCGAAATCGTCCCCGACAGGCGAAACGCCAGCCTGCG
CGCGCTCTGGACCCTTGTGATCGCGCCGGTGCCCAGCTACATCAGAGGGGAACGAGCGAAAGGGAATTTCATGCGCATTG
CACGCGATCTGGCCGATGCGGTCGGCCATACCCCTCTGATCCGACTGAACAAAGTCAGCGAAGAAACCGGTTGCGAAATT
CTTGGCAAGGCGGAGTTTCTGAACCCCGGCCAATCCGTGAAGGACCGCGCCGCGCTCTACATCATCAAGGACGCTATCGC
CCGCGGGGAACTCAAACCCGGCGGCACGATTGTAGAAGGTACAGCGGGCAATACCGGCATTGGGCTTGCGCTGGTCGGCG
CCTCCATGGGCTTCAAGACCGTGATCGTGATCCCTGAAACCCAGTCCGAAGAAAAGAAGGACATGCTGCGTCTGGCGGGG
GCACAGTTGGTTCAGGTGCCTGCAGCGCCTTACAAGAACCCCAACAACTATGTGCGCTACTCCGAGCGCCTGGCAAACGA
GCTGGCCAAGACAGAGCCGAATGGCGCCATCTGGGCCAATCAGTTTGACAATGTGGCCAACCGTCAGGCCCATGTGGAAA
CCACCGGTCCCGAGATCTGGGAGCAGACGGGCGGCAAGGTGGATGGTTTCATCTGTGCTGTTGGCTCCGGCGGGACGCTT
GCCGGGATTGCCGAGGCGCTGCAGCCCAAGGGCGTGAAGATCGGCCTTGCCGATCCCAATGGCGCCGCGCTTTATTCCTA
CTACACCACTGGCGAGCTGAAGTCCGAGGGCTCCTCGATCACAGAAGGGATCGGTCAGGGCCGGATCACCAAGAACCTCG
AGGGGCTCACCCCAGATTTCAACTACCAGATTCCGGACGCAGAGGCGCTGCCTTATGTGTTTGACCTTCTGCACGAAGAG
GGGCTGGTGCTTGGCGGATCTTCCGGCGTGAACATTGCCGGCGCAGTGCGTCTGGCCAAGGAACTGGGGCCGGGGCACAC
CATCGTTACCATCCTGTGCGACTATGGAACGCGCTATCAGTCCAAGCTCTTTAATCCGGAATTCCTCAAAGACAAGGGCT
TGCCGGTCCCCGACTGGATGACCCATACTCCTGCTTCAATTCCCGGAGTATTCGAGGACATCTGA

Upstream 100 bases:

>100_bases
ATGGATCATGCTGCGCCCGCCCAAGAGCGATTAAAGTCAGCACGTTCCGCAAAACCCCACACAGCATGGACAGTGTTGTG
ATGATGCCCGCCGCGGCGCG

Downstream 100 bases:

>100_bases
ATCAGATGACTTATTTGAGAACGCTTTTTCAGGTCGGCCTTTTGGGGCTGACCTGTTTGCTTTTGCCTATTGTTGCTTTG
GCGCAGGAAACCGGCGGGCA

Product: cysteine synthase A

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MVLRASQTQWNHGRAEIVPDRRNASLRALWTLVIAPVPSYIRGERAKGNFMRIARDLADAVGHTPLIRLNKVSEETGCEI
LGKAEFLNPGQSVKDRAALYIIKDAIARGELKPGGTIVEGTAGNTGIGLALVGASMGFKTVIVIPETQSEEKKDMLRLAG
AQLVQVPAAPYKNPNNYVRYSERLANELAKTEPNGAIWANQFDNVANRQAHVETTGPEIWEQTGGKVDGFICAVGSGGTL
AGIAEALQPKGVKIGLADPNGAALYSYYTTGELKSEGSSITEGIGQGRITKNLEGLTPDFNYQIPDAEALPYVFDLLHEE
GLVLGGSSGVNIAGAVRLAKELGPGHTIVTILCDYGTRYQSKLFNPEFLKDKGLPVPDWMTHTPASIPGVFEDI

Sequences:

>Translated_394_residues
MVLRASQTQWNHGRAEIVPDRRNASLRALWTLVIAPVPSYIRGERAKGNFMRIARDLADAVGHTPLIRLNKVSEETGCEI
LGKAEFLNPGQSVKDRAALYIIKDAIARGELKPGGTIVEGTAGNTGIGLALVGASMGFKTVIVIPETQSEEKKDMLRLAG
AQLVQVPAAPYKNPNNYVRYSERLANELAKTEPNGAIWANQFDNVANRQAHVETTGPEIWEQTGGKVDGFICAVGSGGTL
AGIAEALQPKGVKIGLADPNGAALYSYYTTGELKSEGSSITEGIGQGRITKNLEGLTPDFNYQIPDAEALPYVFDLLHEE
GLVLGGSSGVNIAGAVRLAKELGPGHTIVTILCDYGTRYQSKLFNPEFLKDKGLPVPDWMTHTPASIPGVFEDI
>Mature_394_residues
MVLRASQTQWNHGRAEIVPDRRNASLRALWTLVIAPVPSYIRGERAKGNFMRIARDLADAVGHTPLIRLNKVSEETGCEI
LGKAEFLNPGQSVKDRAALYIIKDAIARGELKPGGTIVEGTAGNTGIGLALVGASMGFKTVIVIPETQSEEKKDMLRLAG
AQLVQVPAAPYKNPNNYVRYSERLANELAKTEPNGAIWANQFDNVANRQAHVETTGPEIWEQTGGKVDGFICAVGSGGTL
AGIAEALQPKGVKIGLADPNGAALYSYYTTGELKSEGSSITEGIGQGRITKNLEGLTPDFNYQIPDAEALPYVFDLLHEE
GLVLGGSSGVNIAGAVRLAKELGPGHTIVTILCDYGTRYQSKLFNPEFLKDKGLPVPDWMTHTPASIPGVFEDI

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=359, Percent_Identity=38.7186629526462, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI295821200, Length=359, Percent_Identity=38.7186629526462, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI4557415, Length=359, Percent_Identity=38.7186629526462, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1788754, Length=328, Percent_Identity=37.8048780487805, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI2367138, Length=312, Percent_Identity=35.2564102564103, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17535051, Length=314, Percent_Identity=35.3503184713376, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17562970, Length=323, Percent_Identity=35.9133126934984, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32566674, Length=311, Percent_Identity=36.3344051446945, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17561720, Length=329, Percent_Identity=34.9544072948328, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI115535073, Length=314, Percent_Identity=35.031847133758, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17534315, Length=355, Percent_Identity=33.5211267605634, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25147552, Length=325, Percent_Identity=31.3846153846154, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566672, Length=231, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71996324, Length=330, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321449, Length=324, Percent_Identity=38.2716049382716, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321594, Length=346, Percent_Identity=32.9479768786127, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24643623, Length=356, Percent_Identity=33.9887640449438, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI20129101, Length=356, Percent_Identity=33.9887640449438, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 42247; Mature: 42247

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRASQTQWNHGRAEIVPDRRNASLRALWTLVIAPVPSYIRGERAKGNFMRIARDLADA
CEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHH
VGHTPLIRLNKVSEETGCEILGKAEFLNPGQSVKDRAALYIIKDAIARGELKPGGTIVEG
HCCCCEEEEECCCCCCCCEEEECHHHCCCCCCCHHHHHEEEEEHHHHCCCCCCCCEEEEC
TAGNTGIGLALVGASMGFKTVIVIPETQSEEKKDMLRLAGAQLVQVPAAPYKNPNNYVRY
CCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
SERLANELAKTEPNGAIWANQFDNVANRQAHVETTGPEIWEQTGGKVDGFICAVGSGGTL
HHHHHHHHHHCCCCCCEECCHHHHHHCCCEEEECCCHHHHHHCCCCCCEEEEEECCCCCH
AGIAEALQPKGVKIGLADPNGAALYSYYTTGELKSEGSSITEGIGQGRITKNLEGLTPDF
HHHHHHHCCCCEEEEECCCCCCEEEEEEECCCHHCCCCHHHHHCCCCCEECCCCCCCCCC
NYQIPDAEALPYVFDLLHEEGLVLGGSSGVNIAGAVRLAKELGPGHTIVTILCDYGTRYQ
CEECCCCCHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
SKLFNPEFLKDKGLPVPDWMTHTPASIPGVFEDI
HHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MVLRASQTQWNHGRAEIVPDRRNASLRALWTLVIAPVPSYIRGERAKGNFMRIARDLADA
CEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHH
VGHTPLIRLNKVSEETGCEILGKAEFLNPGQSVKDRAALYIIKDAIARGELKPGGTIVEG
HCCCCEEEEECCCCCCCCEEEECHHHCCCCCCCHHHHHEEEEEHHHHCCCCCCCCEEEEC
TAGNTGIGLALVGASMGFKTVIVIPETQSEEKKDMLRLAGAQLVQVPAAPYKNPNNYVRY
CCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
SERLANELAKTEPNGAIWANQFDNVANRQAHVETTGPEIWEQTGGKVDGFICAVGSGGTL
HHHHHHHHHHCCCCCCEECCHHHHHHCCCEEEECCCHHHHHHCCCCCCEEEEEECCCCCH
AGIAEALQPKGVKIGLADPNGAALYSYYTTGELKSEGSSITEGIGQGRITKNLEGLTPDF
HHHHHHHCCCCEEEEECCCCCCEEEEEEECCCHHCCCCHHHHHCCCCCEECCCCCCCCCC
NYQIPDAEALPYVFDLLHEEGLVLGGSSGVNIAGAVRLAKELGPGHTIVTILCDYGTRYQ
CEECCCCCHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
SKLFNPEFLKDKGLPVPDWMTHTPASIPGVFEDI
HHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]