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The map label for this gene is nikA [H]

Identifier: 99081258

GI number: 99081258

Start: 1510334

End: 1512001

Strand: Direct

Name: nikA [H]

Synonym: TM1040_1417

Alternate gene names: 99081258

Gene position: 1510334-1512001 (Clockwise)

Preceding gene: 99081257

Following gene: 99081259

Centisome position: 47.18

GC content: 60.01

Gene sequence:

>1668_bases
ATGACCACCACCAAACTCAAGAGCTCGACGACCCATCATTGGGCGGCTGAGATGCATGCGCAGGAACTGAAGGCGGGCAA
GATCTCGCGCCGCGAGTTCCTGACCCGTGCGACAGCGCTTGGTGTCTCGGCCACCGCCGCCTATGGGATGATCGGCCTGA
GCGCCCCTGCCCGTGCGGCGGACAACATGAAAGAAGGCGGCACCCTGCGCGTGCAAATGAGCGTGCGCGGCCTCAAGGAT
CCCCGCACCTTCGACTGGTCCGAAATCGGCAACCAGGCGCGTGGCACGCTTGAATACCTGGTTGAATACAACAACGACGG
CTCCTTCACCCCGATGCTCCTGGAGAGCTGGCAGGTGAATGACAGCGCCGATGAATACACCCTGAACGTTCGCAAGGGCG
TCAAATGGAACAACGGCGACGACTTTACCGCCGAAGATGTGGCCCGCAACATCGAAGGTTGGTGCGACAAGGACATGGAA
GGCAACTCCATGGCTGGCCGCTTTGCCACGCTTGTCGATCCCGACACCGGCAAAGCCATCGAGGGCGCGATCGAGGTGGT
AGACAGCCACACCGTGAAACTGAAACTGCCTAACTCCGACATTTCCATCATTCCGGGCATGGCGGATTATCCGGCAGCGA
TCACCCATGCGAGCTTTAGCGCGGATGATGCGCAAAACGCCGTGGGCACCGGTCCCTATCTGATTTCCGAGCTCGAAGTC
GGCGTCAAATGCGTGCTCACGCGCAATGAGAACCACAGCTGGTGGGGTGAAGCGATCTACGGCAAGCCGGCGCTCGACAG
CATCGAATACATCGATTTCGGCACCGATCCGTCGTCGTGGCTGGCAGCGCTGGAATCCGAAGAAGTCGATATGCTCTATG
AATCCGTTGGCGAATTCATCGACTTGATGGACTCGCTTGGCTTCGTGAAATCCGAAGTTGTCACCATGGCAACCATCGTC
GTGCGTCCCAACCAGCTGGCCGAGGTGGACGGCATGTCCCCCTATGCCGACAAACGCGTGCGCAAGGCGCTGCAGATGGC
GGTGGACAATGATGTCTGCCTGGAGCTTGGCTATGGCAACCGCGGTGTTGCTGCGGAAAACCATCACGTCGGCCCGGCGC
ATCCTGAATATGCCGAGCTCCCTAAAGAGGCGGCTGATCCCGATGGCGCCCGCGCGCTGATGGAAGAAGCCGGCATGATG
GATTTCGAACATGAGCTCTTCTCCATCGACGATGACTGGCGCAAGAACACCACCGATGCGGTCGCTGCCCAGCTGCGTGA
CGCGGGGTTCAAGGTCAAGCGGACCGTGCTGCCGGGATCGACCTTCTGGAATGACTGGACCAAATATCCGTTCAGCTCGA
CCAACTGGAACCACCGTCCCTTGGGTGTTCAGATCTGGGCGCTGGCCTACCGTTCAGGTGAGGCCTGGAACGAATTCGGC
TGGTCCAACCCCGAGTTCGACCAGATCCTGGCCGACGCGCTTGCCATCGCCGATGCCGACGCGCGCCGCGAAGTCGTCGC
CAAAGGTCAGAAGATGATCCAGGACGAGGGTGTCACCATCCAGCCCTATTGGAAATCCCTGTTCCGTCACATGCGCGAAG
GCGTGCGCGGTGCCGATATGCATATCTCCTACGAGCACCATCACTACAAATGGGGCTGGGCTGCCTGA

Upstream 100 bases:

>100_bases
CACCTCCGTATGATGCGTGCCTTTTGGATCGACCGCAGATCGCACCGCCGCCTCATGGGAAGGTCGCCGCCCAACGACGC
TCTAGTACAGGGAGAAATAT

Downstream 100 bases:

>100_bases
TCGCGCCCCCTTTACCTAAAGGCCTGTCCCCAATCCGTTTTTCGGACCGGATTGGGGGCAGGCCCAACGCACCACGCCTG
AATTCAAAACAATAAAGGCC

Product: twin-arginine translocation pathway signal

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTTTKLKSSTTHHWAAEMHAQELKAGKISRREFLTRATALGVSATAAYGMIGLSAPARAADNMKEGGTLRVQMSVRGLKD
PRTFDWSEIGNQARGTLEYLVEYNNDGSFTPMLLESWQVNDSADEYTLNVRKGVKWNNGDDFTAEDVARNIEGWCDKDME
GNSMAGRFATLVDPDTGKAIEGAIEVVDSHTVKLKLPNSDISIIPGMADYPAAITHASFSADDAQNAVGTGPYLISELEV
GVKCVLTRNENHSWWGEAIYGKPALDSIEYIDFGTDPSSWLAALESEEVDMLYESVGEFIDLMDSLGFVKSEVVTMATIV
VRPNQLAEVDGMSPYADKRVRKALQMAVDNDVCLELGYGNRGVAAENHHVGPAHPEYAELPKEAADPDGARALMEEAGMM
DFEHELFSIDDDWRKNTTDAVAAQLRDAGFKVKRTVLPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFG
WSNPEFDQILADALAIADADARREVVAKGQKMIQDEGVTIQPYWKSLFRHMREGVRGADMHISYEHHHYKWGWAA

Sequences:

>Translated_555_residues
MTTTKLKSSTTHHWAAEMHAQELKAGKISRREFLTRATALGVSATAAYGMIGLSAPARAADNMKEGGTLRVQMSVRGLKD
PRTFDWSEIGNQARGTLEYLVEYNNDGSFTPMLLESWQVNDSADEYTLNVRKGVKWNNGDDFTAEDVARNIEGWCDKDME
GNSMAGRFATLVDPDTGKAIEGAIEVVDSHTVKLKLPNSDISIIPGMADYPAAITHASFSADDAQNAVGTGPYLISELEV
GVKCVLTRNENHSWWGEAIYGKPALDSIEYIDFGTDPSSWLAALESEEVDMLYESVGEFIDLMDSLGFVKSEVVTMATIV
VRPNQLAEVDGMSPYADKRVRKALQMAVDNDVCLELGYGNRGVAAENHHVGPAHPEYAELPKEAADPDGARALMEEAGMM
DFEHELFSIDDDWRKNTTDAVAAQLRDAGFKVKRTVLPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFG
WSNPEFDQILADALAIADADARREVVAKGQKMIQDEGVTIQPYWKSLFRHMREGVRGADMHISYEHHHYKWGWAA
>Mature_554_residues
TTTKLKSSTTHHWAAEMHAQELKAGKISRREFLTRATALGVSATAAYGMIGLSAPARAADNMKEGGTLRVQMSVRGLKDP
RTFDWSEIGNQARGTLEYLVEYNNDGSFTPMLLESWQVNDSADEYTLNVRKGVKWNNGDDFTAEDVARNIEGWCDKDMEG
NSMAGRFATLVDPDTGKAIEGAIEVVDSHTVKLKLPNSDISIIPGMADYPAAITHASFSADDAQNAVGTGPYLISELEVG
VKCVLTRNENHSWWGEAIYGKPALDSIEYIDFGTDPSSWLAALESEEVDMLYESVGEFIDLMDSLGFVKSEVVTMATIVV
RPNQLAEVDGMSPYADKRVRKALQMAVDNDVCLELGYGNRGVAAENHHVGPAHPEYAELPKEAADPDGARALMEEAGMMD
FEHELFSIDDDWRKNTTDAVAAQLRDAGFKVKRTVLPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFGW
SNPEFDQILADALAIADADARREVVAKGQKMIQDEGVTIQPYWKSLFRHMREGVRGADMHISYEHHHYKWGWAA

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=308, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787551, Length=282, Percent_Identity=25.886524822695, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61634; Mature: 61503

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTKLKSSTTHHWAAEMHAQELKAGKISRREFLTRATALGVSATAAYGMIGLSAPARAA
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHEECCCCCHHH
DNMKEGGTLRVQMSVRGLKDPRTFDWSEIGNQARGTLEYLVEYNNDGSFTPMLLESWQVN
CCCCCCCEEEEEEEECCCCCCCCCCHHHHCCHHCCEEEEEEEECCCCCCCHHHHCCCCCC
DSADEYTLNVRKGVKWNNGDDFTAEDVARNIEGWCDKDMEGNSMAGRFATLVDPDTGKAI
CCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHH
EGAIEVVDSHTVKLKLPNSDISIIPGMADYPAAITHASFSADDAQNAVGTGPYLISELEV
HHHHHHCCCCEEEEEECCCCCEEECCCCCCCHHHEECCCCCCCHHCCCCCCCHHHHHHCC
GVKCVLTRNENHSWWGEAIYGKPALDSIEYIDFGTDPSSWLAALESEEVDMLYESVGEFI
CEEEEEEECCCCCCCCCEECCCCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHH
DLMDSLGFVKSEVVTMATIVVRPNQLAEVDGMSPYADKRVRKALQMAVDNDVCLELGYGN
HHHHHHHHHHHHHHEEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
RGVAAENHHVGPAHPEYAELPKEAADPDGARALMEEAGMMDFEHELFSIDDDWRKNTTDA
CCCCCCCCCCCCCCCHHHHCCHHHCCCCHHHHHHHHCCCCCCCHHHCCCCCHHCCCCHHH
VAAQLRDAGFKVKRTVLPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFG
HHHHHHHCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHCC
WSNPEFDQILADALAIADADARREVVAKGQKMIQDEGVTIQPYWKSLFRHMREGVRGADM
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCE
HISYEHHHYKWGWAA
EEEECCCEEECCCCC
>Mature Secondary Structure 
TTTKLKSSTTHHWAAEMHAQELKAGKISRREFLTRATALGVSATAAYGMIGLSAPARAA
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHEECCCCCHHH
DNMKEGGTLRVQMSVRGLKDPRTFDWSEIGNQARGTLEYLVEYNNDGSFTPMLLESWQVN
CCCCCCCEEEEEEEECCCCCCCCCCHHHHCCHHCCEEEEEEEECCCCCCCHHHHCCCCCC
DSADEYTLNVRKGVKWNNGDDFTAEDVARNIEGWCDKDMEGNSMAGRFATLVDPDTGKAI
CCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHH
EGAIEVVDSHTVKLKLPNSDISIIPGMADYPAAITHASFSADDAQNAVGTGPYLISELEV
HHHHHHCCCCEEEEEECCCCCEEECCCCCCCHHHEECCCCCCCHHCCCCCCCHHHHHHCC
GVKCVLTRNENHSWWGEAIYGKPALDSIEYIDFGTDPSSWLAALESEEVDMLYESVGEFI
CEEEEEEECCCCCCCCCEECCCCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHH
DLMDSLGFVKSEVVTMATIVVRPNQLAEVDGMSPYADKRVRKALQMAVDNDVCLELGYGN
HHHHHHHHHHHHHHEEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
RGVAAENHHVGPAHPEYAELPKEAADPDGARALMEEAGMMDFEHELFSIDDDWRKNTTDA
CCCCCCCCCCCCCCCHHHHCCHHHCCCCHHHHHHHHCCCCCCCHHHCCCCCHHCCCCHHH
VAAQLRDAGFKVKRTVLPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFG
HHHHHHHCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHCC
WSNPEFDQILADALAIADADARREVVAKGQKMIQDEGVTIQPYWKSLFRHMREGVRGADM
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCE
HISYEHHHYKWGWAA
EEEECCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]