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The map label for this gene is speE

Identifier: 99081230

GI number: 99081230

Start: 1482669

End: 1483523

Strand: Direct

Name: speE

Synonym: TM1040_1389

Alternate gene names: 99081230

Gene position: 1482669-1483523 (Clockwise)

Preceding gene: 99081229

Following gene: 99081232

Centisome position: 46.32

GC content: 57.43

Gene sequence:

>855_bases
ATGACGAAAGAGGTCTGGAACACAGAACGCCTGCACGATCATTTCGCCCAATCCCTGCGCGTGAGCGAGATGTATTATGA
CAGCAAGACCGAGCACCAGCGGCTCAAGGTCTTTCAGAATGGCACCTTCGGGCGCGTTCTGACGCTGGACGATGTGGTAC
AGACCACCGAAGGCGACAACTTCATCTATCATGAAATGCTGACCCATGTGCCGATCCTGGCACATGGGGCCGCCAAACGT
GTGCTCATCATCGGTGGCGGCGACGGTGGCATTGCGCGCGAGGTGCTGAAACACGCCTCCGTCGAGCATGTCACCATGGT
GGAAATCGACGCTGGCGTTGTCGATTTTTCCAAGGAATACCTGCCGATGCTGAGCCAGGGCGCATTTGACGATGCGCGTC
TCAACCTGGTGATCAATGACGGTGCGGTCTTTATGAAAGAGACCGAAGATAAATTCGACGTCATCATTGTCGACTCGACC
GACCCCATCGGCCCCGGCGAAGTGCTGTTTACCGATACATTCTATGGCCATGCAGCGCGTGCGCTAACCGAGGATGGCAT
CATCGTCACCCAGAACGGCGTGCCGTTCATGCAGGGCGATGAGCTGACCAACACGATGCGGGCCTTTCAAGCGCTGTTTC
AGGATGCGACCTGCTATCTCGCGACGATTCCGACCTACGTCGGCGGCCCGATGGCATTGGGCTGGGGTAGCCACTCCACA
AAGGCGCGTTCGGTGGATCTGACAACGCTTGAAGCTCGGTTTGCAGCGGCAGGCCTTTCGCCGGACTACTACACCCCAGA
GGTGCACAAGGCGGCATTCGCGCTGCCCGGATACGTCAAGAAACTGTTTCCATAA

Upstream 100 bases:

>100_bases
GGAAAGCCGTCGATGTGCTAAAAGCCGCCTTTGAGACCGATGTGGTAGAAGTGCGCGAATTGCTGCGCGGCGAGGAAATC
GTCGCCAAGGAGATCGCAGC

Downstream 100 bases:

>100_bases
GGTAGCTTACTTAGCGACCTTAAAGCAAACGCGTCCCGGTTCCTACCTCGGGCGCGTTTAGCGTTTCGGCGGGATGGAAT
AGGTCATGGTTGAGCGCGCG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTKEVWNTERLHDHFAQSLRVSEMYYDSKTEHQRLKVFQNGTFGRVLTLDDVVQTTEGDNFIYHEMLTHVPILAHGAAKR
VLIIGGGDGGIAREVLKHASVEHVTMVEIDAGVVDFSKEYLPMLSQGAFDDARLNLVINDGAVFMKETEDKFDVIIVDST
DPIGPGEVLFTDTFYGHAARALTEDGIIVTQNGVPFMQGDELTNTMRAFQALFQDATCYLATIPTYVGGPMALGWGSHST
KARSVDLTTLEARFAAAGLSPDYYTPEVHKAAFALPGYVKKLFP

Sequences:

>Translated_284_residues
MTKEVWNTERLHDHFAQSLRVSEMYYDSKTEHQRLKVFQNGTFGRVLTLDDVVQTTEGDNFIYHEMLTHVPILAHGAAKR
VLIIGGGDGGIAREVLKHASVEHVTMVEIDAGVVDFSKEYLPMLSQGAFDDARLNLVINDGAVFMKETEDKFDVIIVDST
DPIGPGEVLFTDTFYGHAARALTEDGIIVTQNGVPFMQGDELTNTMRAFQALFQDATCYLATIPTYVGGPMALGWGSHST
KARSVDLTTLEARFAAAGLSPDYYTPEVHKAAFALPGYVKKLFP
>Mature_283_residues
TKEVWNTERLHDHFAQSLRVSEMYYDSKTEHQRLKVFQNGTFGRVLTLDDVVQTTEGDNFIYHEMLTHVPILAHGAAKRV
LIIGGGDGGIAREVLKHASVEHVTMVEIDAGVVDFSKEYLPMLSQGAFDDARLNLVINDGAVFMKETEDKFDVIIVDSTD
PIGPGEVLFTDTFYGHAARALTEDGIIVTQNGVPFMQGDELTNTMRAFQALFQDATCYLATIPTYVGGPMALGWGSHSTK
ARSVDLTTLEARFAAAGLSPDYYTPEVHKAAFALPGYVKKLFP

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=282, Percent_Identity=39.3617021276596, Blast_Score=207, Evalue=9e-54,
Organism=Escherichia coli, GI1786312, Length=278, Percent_Identity=53.2374100719424, Blast_Score=306, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI25154106, Length=266, Percent_Identity=43.609022556391, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI212645616, Length=317, Percent_Identity=38.801261829653, Blast_Score=178, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323175, Length=277, Percent_Identity=38.2671480144404, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6325326, Length=266, Percent_Identity=36.4661654135338, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24645443, Length=267, Percent_Identity=40.4494382022472, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21355941, Length=219, Percent_Identity=41.0958904109589, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21358269, Length=185, Percent_Identity=28.1081081081081, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24663238, Length=185, Percent_Identity=28.1081081081081, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_SILST (Q1GGU4)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613384.1
- ProteinModelPortal:   Q1GGU4
- SMR:   Q1GGU4
- STRING:   Q1GGU4
- GeneID:   4075882
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1389
- NMPDR:   fig|292414.1.peg.3505
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   FAWASNN
- PhylomeDB:   Q1GGU4
- ProtClustDB:   PRK00811
- BioCyc:   SSP292414:TM1040_1389-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 31325; Mature: 31194

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 33-33 BINDING 88-88 BINDING 108-108 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEVWNTERLHDHFAQSLRVSEMYYDSKTEHQRLKVFQNGTFGRVLTLDDVVQTTEGDN
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEHHHHHHHCCCCC
FIYHEMLTHVPILAHGAAKRVLIIGGGDGGIAREVLKHASVEHVTMVEIDAGVVDFSKEY
CHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCEECCCHHH
LPMLSQGAFDDARLNLVINDGAVFMKETEDKFDVIIVDSTDPIGPGEVLFTDTFYGHAAR
HHHHHCCCCCCEEEEEEEECCEEEEEECCCCEEEEEEECCCCCCCCCEEEEECHHHHHHH
ALTEDGIIVTQNGVPFMQGDELTNTMRAFQALFQDATCYLATIPTYVGGPMALGWGSHST
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHCCCCEEECCCCCCC
KARSVDLTTLEARFAAAGLSPDYYTPEVHKAAFALPGYVKKLFP
CCEEEEEEEHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TKEVWNTERLHDHFAQSLRVSEMYYDSKTEHQRLKVFQNGTFGRVLTLDDVVQTTEGDN
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEHHHHHHHCCCCC
FIYHEMLTHVPILAHGAAKRVLIIGGGDGGIAREVLKHASVEHVTMVEIDAGVVDFSKEY
CHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCEECCCHHH
LPMLSQGAFDDARLNLVINDGAVFMKETEDKFDVIIVDSTDPIGPGEVLFTDTFYGHAAR
HHHHHCCCCCCEEEEEEEECCEEEEEECCCCEEEEEEECCCCCCCCCEEEEECHHHHHHH
ALTEDGIIVTQNGVPFMQGDELTNTMRAFQALFQDATCYLATIPTYVGGPMALGWGSHST
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHCCCCEEECCCCCCC
KARSVDLTTLEARFAAAGLSPDYYTPEVHKAAFALPGYVKKLFP
CCEEEEEEEHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA