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The map label for this gene is trmFO [H]

Identifier: 99081232

GI number: 99081232

Start: 1484388

End: 1485740

Strand: Direct

Name: trmFO [H]

Synonym: TM1040_1391

Alternate gene names: 99081232

Gene position: 1484388-1485740 (Clockwise)

Preceding gene: 99081230

Following gene: 99081233

Centisome position: 46.37

GC content: 60.75

Gene sequence:

>1353_bases
ATGTGGCCTTTCATGACACAAGAGCTTCATATCGTCGGCGGCGGAATGGCCGGATCGGAAGCCGCCTGGCAGGCGGCGCA
CATGGGCGTTGACGTGGTGATCCACGAGATGCGGCCCAAGGTTGAAACCTTTGCCCATCAGACCGGCAACCTCGCCGAGA
TGGTCTGCTCCAACTCGTTCCGCTCGGATGATGACGAGCAGAATGCAGTGGGGCTTTTGCATTGGGAAATGCGCGCGGCC
AACAGTCTCATTATGACCACCGCAGGTGAACACCGGCTTCCGGCTGGGGGTGCGTTGGCCGTGGATCGCGATCCCTTTGC
GGAATCCGTGACCGCCAAACTCAAGGCGCTGCCCAATGTGCGCGTGTCCTACGAGGAAGTCACAGACCTCCCCACCGAGG
GGCACTGGATCTTTGCCACCGGTCCCCTCACCTCGCCCGCGCTTGGCGAAGCGATCCAGCGCGAAACTGGGGCAGAACGG
TTGGCATTCTTTGATGCCATCGCGCCCATCGTCTATGCCGAGAGTATCGACATGTCCCAAGCCTGGATGCAGTCGCGCTA
TGACAAGGGCGAAACCGAGGAAGAGCGCACCGCCTACCTCAACTGCCCGATGACCAAGGACCAATACGAGGCCTTCATCG
ACGCGCTTCTGGCCGCAGACAAGACCGAGTTCCATGAGGGCGAAACCGCTGGCTATTTCGACGGCTGCCTCCCGATCGAG
GTGATGGCAGAACGCGGCCGCGAGACCCTGCGCCATGGCCCGATGAAGCCCGTTGGCCTTACCAATCCGCACAAGCCGGA
CGAAAAGGCCTGGGCCGTGGTGCAGTTGCGCCGTGACAATGCGCTCGGGACGCTTTTCAACATCGTTGGTTTTCAGACCA
AGATGAAATACGGCGCGCAAACGGATGTCTTCCGGATGATCCCCGGCCTCGAGAACGCCAAATTTGCGCGGCTCGGCGGC
ATTCATCGCAACACCTTCCTGAACTCTCCGACGCTGCTCGATCATGAGATGCGCCTGAAATCAAAGCCGAACATTCGGTT
TGCCGGACAGGTGACGGGCGTTGAGGGCTATGTGGAAAGTGCGGCGATGGGCTTGTTGGCCGGGCGTATGGCCGCCGCTG
AGATCCTCGGCAAGGACCTGCCTCAGGTGCCGCAGGACAGCGCCATGGGGGCCTTGATCCACCACATCACCGGCGGCGCC
GAAGCCAAGACTTTTCAGCCGATGAACGTCAATTTTGGCCTGTTCCGCCCGGTCGAGGGGCTGAAAGGTGGACGTCGTGG
CCGCAAGGACCGCTACAAGGCCTATACCGATCGCGCCAAGGTGGCATGGCAGGAATGGCTCACAAATTTTTGA

Upstream 100 bases:

>100_bases
CTCACCCGGATTTGACGTCCTCGAAGCTGCGGCCCAAATAATTTCCCCTCAGAGACAACGTGCGCGCACGACGATATTGC
GCCCGAAGGCCGCCTGCCCT

Downstream 100 bases:

>100_bases
TGGACGCACTCATGATCCCGGCTCTAGACTGGGATCATGAGTGATACATTCCTCGAGAAAACATATGAGTTGAACACAGT
CGAAGAGACTGTCGATCATT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MWPFMTQELHIVGGGMAGSEAAWQAAHMGVDVVIHEMRPKVETFAHQTGNLAEMVCSNSFRSDDDEQNAVGLLHWEMRAA
NSLIMTTAGEHRLPAGGALAVDRDPFAESVTAKLKALPNVRVSYEEVTDLPTEGHWIFATGPLTSPALGEAIQRETGAER
LAFFDAIAPIVYAESIDMSQAWMQSRYDKGETEEERTAYLNCPMTKDQYEAFIDALLAADKTEFHEGETAGYFDGCLPIE
VMAERGRETLRHGPMKPVGLTNPHKPDEKAWAVVQLRRDNALGTLFNIVGFQTKMKYGAQTDVFRMIPGLENAKFARLGG
IHRNTFLNSPTLLDHEMRLKSKPNIRFAGQVTGVEGYVESAAMGLLAGRMAAAEILGKDLPQVPQDSAMGALIHHITGGA
EAKTFQPMNVNFGLFRPVEGLKGGRRGRKDRYKAYTDRAKVAWQEWLTNF

Sequences:

>Translated_450_residues
MWPFMTQELHIVGGGMAGSEAAWQAAHMGVDVVIHEMRPKVETFAHQTGNLAEMVCSNSFRSDDDEQNAVGLLHWEMRAA
NSLIMTTAGEHRLPAGGALAVDRDPFAESVTAKLKALPNVRVSYEEVTDLPTEGHWIFATGPLTSPALGEAIQRETGAER
LAFFDAIAPIVYAESIDMSQAWMQSRYDKGETEEERTAYLNCPMTKDQYEAFIDALLAADKTEFHEGETAGYFDGCLPIE
VMAERGRETLRHGPMKPVGLTNPHKPDEKAWAVVQLRRDNALGTLFNIVGFQTKMKYGAQTDVFRMIPGLENAKFARLGG
IHRNTFLNSPTLLDHEMRLKSKPNIRFAGQVTGVEGYVESAAMGLLAGRMAAAEILGKDLPQVPQDSAMGALIHHITGGA
EAKTFQPMNVNFGLFRPVEGLKGGRRGRKDRYKAYTDRAKVAWQEWLTNF
>Mature_450_residues
MWPFMTQELHIVGGGMAGSEAAWQAAHMGVDVVIHEMRPKVETFAHQTGNLAEMVCSNSFRSDDDEQNAVGLLHWEMRAA
NSLIMTTAGEHRLPAGGALAVDRDPFAESVTAKLKALPNVRVSYEEVTDLPTEGHWIFATGPLTSPALGEAIQRETGAER
LAFFDAIAPIVYAESIDMSQAWMQSRYDKGETEEERTAYLNCPMTKDQYEAFIDALLAADKTEFHEGETAGYFDGCLPIE
VMAERGRETLRHGPMKPVGLTNPHKPDEKAWAVVQLRRDNALGTLFNIVGFQTKMKYGAQTDVFRMIPGLENAKFARLGG
IHRNTFLNSPTLLDHEMRLKSKPNIRFAGQVTGVEGYVESAAMGLLAGRMAAAEILGKDLPQVPQDSAMGALIHHITGGA
EAKTFQPMNVNFGLFRPVEGLKGGRRGRKDRYKAYTDRAKVAWQEWLTNF

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49728; Mature: 49728

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWPFMTQELHIVGGGMAGSEAAWQAAHMGVDVVIHEMRPKVETFAHQTGNLAEMVCSNSF
CCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCC
RSDDDEQNAVGLLHWEMRAANSLIMTTAGEHRLPAGGALAVDRDPFAESVTAKLKALPNV
CCCCCCCCCEEEEEEEHHHCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCC
RVSYEEVTDLPTEGHWIFATGPLTSPALGEAIQRETGAERLAFFDAIAPIVYAESIDMSQ
EEEHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHH
AWMQSRYDKGETEEERTAYLNCPMTKDQYEAFIDALLAADKTEFHEGETAGYFDGCLPIE
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHH
VMAERGRETLRHGPMKPVGLTNPHKPDEKAWAVVQLRRDNALGTLFNIVGFQTKMKYGAQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
TDVFRMIPGLENAKFARLGGIHRNTFLNSPTLLDHEMRLKSKPNIRFAGQVTGVEGYVES
CHHHHHCCCCCCCCHHHHCCCHHHHCCCCCCEECHHHHHCCCCCEEEEEEECCHHHHHHH
AAMGLLAGRMAAAEILGKDLPQVPQDSAMGALIHHITGGAEAKTFQPMNVNFGLFRPVEG
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHH
LKGGRRGRKDRYKAYTDRAKVAWQEWLTNF
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWPFMTQELHIVGGGMAGSEAAWQAAHMGVDVVIHEMRPKVETFAHQTGNLAEMVCSNSF
CCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCC
RSDDDEQNAVGLLHWEMRAANSLIMTTAGEHRLPAGGALAVDRDPFAESVTAKLKALPNV
CCCCCCCCCEEEEEEEHHHCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCC
RVSYEEVTDLPTEGHWIFATGPLTSPALGEAIQRETGAERLAFFDAIAPIVYAESIDMSQ
EEEHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHH
AWMQSRYDKGETEEERTAYLNCPMTKDQYEAFIDALLAADKTEFHEGETAGYFDGCLPIE
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHH
VMAERGRETLRHGPMKPVGLTNPHKPDEKAWAVVQLRRDNALGTLFNIVGFQTKMKYGAQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
TDVFRMIPGLENAKFARLGGIHRNTFLNSPTLLDHEMRLKSKPNIRFAGQVTGVEGYVES
CHHHHHCCCCCCCCHHHHCCCHHHHCCCCCCEECHHHHHCCCCCEEEEEEECCHHHHHHH
AAMGLLAGRMAAAEILGKDLPQVPQDSAMGALIHHITGGAEAKTFQPMNVNFGLFRPVEG
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHH
LKGGRRGRKDRYKAYTDRAKVAWQEWLTNF
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA