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The map label for this gene is lipA [H]

Identifier: 99081212

GI number: 99081212

Start: 1463741

End: 1464733

Strand: Reverse

Name: lipA [H]

Synonym: TM1040_1371

Alternate gene names: 99081212

Gene position: 1464733-1463741 (Counterclockwise)

Preceding gene: 99081213

Following gene: 99081211

Centisome position: 45.76

GC content: 58.21

Gene sequence:

>993_bases
ATGTTGCGCCCAAATACCTGTCGCAGTCACAAGGAAGACCCTGTGAGAGATCTGAAAATCCCGGAACAGCGCCACCCGGA
AAAGGCGCACCGTCCCGATAATGCTCAGCCCAAAAAGCCAAGCTGGATTCGCGTCAAGGCGCCAGGTGGCAAGGGCTATG
CCGAGACGCATAAAATTATGAGGGAAAACAACCTCGTAACCGTGTGCGAAGAGGCGGGCTGCCCCAATGTGGGCGAATGC
TGGAGCCAGGGCCACGCAACCATGATGATCATGGGTGAAATCTGTACTCGTGGCTGCACCTTCTGCAATATTGCAACCGG
TCGCCCCGACACTCTGGATGCGTTTGAGCCTGGGCGCGTGGCGCATGCGGTGCAAAAACTCGGCCTCAACCATGTTGTGA
TCACATCCGTGGACCGCGACGATCTAGAGGATGGCGGTGCCGATCACTTTGCGCAAACGATCCGCGCGGTGCGTCATCGC
TCGCCGCAGACCACGATCGAGATCCTCACCCCGGACTTTCTGAAATGTGCCCCTGAGGTGCTGGAAACGGTTGTCGAGGC
CAAGCCAGACGTGTTCAACCACAACCTCGAAACCGTTCCCGGGCTCTATCCCGAGGTGCGGCCAGGTGCGCGTTATTTCC
ACTCGCTGCGGTTGTTGCAGCGCGTCAAAGAGCTCGATCCGAGCATCTTTACCAAATCGGGCATCATGGTGGGGCTGGGA
GAGCAGGCGCCTCAGGTGAAACAGGTGATGGATGACATGCGTGCGGCGGATGTTGATTTCCTGACCATCGGCCAATATCT
GCAGCCGACGCCCAAGCACCATGCGGTGGATCGGTTCGTGACACCGGAAGAGTTTGAGTCATACGAAAAAGCAGCCTATG
GCAAAGGCTTTCTGATGGTGTCGGCAACACCGCTGACCCGGTCGTCCTATCACGCGGGCGATGATTTCGCCAAGCTGCGC
GCGGCGCGGAATGCCAAGCTTGGGCTTGCATGA

Upstream 100 bases:

>100_bases
GGGCAAGTGTCCGCGCGCCTGAGTTCCAAGTCCGTTGCAGAACCAAGAGCGACACATCGTTATTGCGAAGCGGCACTGGT
GGAGCAGGGCAGGGACTGTT

Downstream 100 bases:

>100_bases
CGCCTGACGAACTGGATCGGTTGATCGCACTGCGCCATGCACTTCATTCAGCGCCGGAACTCTCTGGCGCTGAACAGGAA
ACGGCTGCCCGCATCCTGTC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MLRPNTCRSHKEDPVRDLKIPEQRHPEKAHRPDNAQPKKPSWIRVKAPGGKGYAETHKIMRENNLVTVCEEAGCPNVGEC
WSQGHATMMIMGEICTRGCTFCNIATGRPDTLDAFEPGRVAHAVQKLGLNHVVITSVDRDDLEDGGADHFAQTIRAVRHR
SPQTTIEILTPDFLKCAPEVLETVVEAKPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPSIFTKSGIMVGLG
EQAPQVKQVMDDMRAADVDFLTIGQYLQPTPKHHAVDRFVTPEEFESYEKAAYGKGFLMVSATPLTRSSYHAGDDFAKLR
AARNAKLGLA

Sequences:

>Translated_330_residues
MLRPNTCRSHKEDPVRDLKIPEQRHPEKAHRPDNAQPKKPSWIRVKAPGGKGYAETHKIMRENNLVTVCEEAGCPNVGEC
WSQGHATMMIMGEICTRGCTFCNIATGRPDTLDAFEPGRVAHAVQKLGLNHVVITSVDRDDLEDGGADHFAQTIRAVRHR
SPQTTIEILTPDFLKCAPEVLETVVEAKPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPSIFTKSGIMVGLG
EQAPQVKQVMDDMRAADVDFLTIGQYLQPTPKHHAVDRFVTPEEFESYEKAAYGKGFLMVSATPLTRSSYHAGDDFAKLR
AARNAKLGLA
>Mature_330_residues
MLRPNTCRSHKEDPVRDLKIPEQRHPEKAHRPDNAQPKKPSWIRVKAPGGKGYAETHKIMRENNLVTVCEEAGCPNVGEC
WSQGHATMMIMGEICTRGCTFCNIATGRPDTLDAFEPGRVAHAVQKLGLNHVVITSVDRDDLEDGGADHFAQTIRAVRHR
SPQTTIEILTPDFLKCAPEVLETVVEAKPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPSIFTKSGIMVGLG
EQAPQVKQVMDDMRAADVDFLTIGQYLQPTPKHHAVDRFVTPEEFESYEKAAYGKGFLMVSATPLTRSSYHAGDDFAKLR
AARNAKLGLA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=295, Percent_Identity=47.1186440677966, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=47.7551020408163, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=51.0869565217391, Blast_Score=288, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=267, Percent_Identity=46.8164794007491, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=43.4343434343434, Blast_Score=262, Evalue=4e-71,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=46.1267605633803, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36740; Mature: 36740

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPNTCRSHKEDPVRDLKIPEQRHPEKAHRPDNAQPKKPSWIRVKAPGGKGYAETHKIM
CCCCCCCCCCCCCCHHHCCCCHHCCCHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
RENNLVTVCEEAGCPNVGECWSQGHATMMIMGEICTRGCTFCNIATGRPDTLDAFEPGRV
HCCCEEEEECCCCCCCHHHHHHCCCEEEEEHHHHHHCCCCEEEECCCCCCCCCCCCCHHH
AHAVQKLGLNHVVITSVDRDDLEDGGADHFAQTIRAVRHRSPQTTIEILTPDFLKCAPEV
HHHHHHCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
LETVVEAKPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPSIFTKSGIMVGLG
HHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHEECCCEEEECC
EQAPQVKQVMDDMRAADVDFLTIGQYLQPTPKHHAVDRFVTPEEFESYEKAAYGKGFLMV
CCCHHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEE
SATPLTRSSYHAGDDFAKLRAARNAKLGLA
ECCCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLRPNTCRSHKEDPVRDLKIPEQRHPEKAHRPDNAQPKKPSWIRVKAPGGKGYAETHKIM
CCCCCCCCCCCCCCHHHCCCCHHCCCHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
RENNLVTVCEEAGCPNVGECWSQGHATMMIMGEICTRGCTFCNIATGRPDTLDAFEPGRV
HCCCEEEEECCCCCCCHHHHHHCCCEEEEEHHHHHHCCCCEEEECCCCCCCCCCCCCHHH
AHAVQKLGLNHVVITSVDRDDLEDGGADHFAQTIRAVRHRSPQTTIEILTPDFLKCAPEV
HHHHHHCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
LETVVEAKPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPSIFTKSGIMVGLG
HHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHEECCCEEEECC
EQAPQVKQVMDDMRAADVDFLTIGQYLQPTPKHHAVDRFVTPEEFESYEKAAYGKGFLMV
CCCHHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEE
SATPLTRSSYHAGDDFAKLRAARNAKLGLA
ECCCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA