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The map label for this gene is garR [C]

Identifier: 99081194

GI number: 99081194

Start: 1443294

End: 1444166

Strand: Reverse

Name: garR [C]

Synonym: TM1040_1353

Alternate gene names: 99081194

Gene position: 1444166-1443294 (Counterclockwise)

Preceding gene: 99081196

Following gene: 99081191

Centisome position: 45.12

GC content: 60.6

Gene sequence:

>873_bases
ATGGCAAAAATTGCGTTCCTTGGGCTCGGGGTCATGGGGTATCCGATGGCCGGGCATCTGCAAAAGGCCGGGCATGAGGT
CACGGTTTACAACCGCACAACTGCAAAGGCCGAAGGCTGGGCGGCAGCGACGGGCGGTCGGTCTGCGCCAACGCCCAGAG
AGGCCGCACAAAACGCGGATTTTGTCATGGCCTGTGTGGGCAATGACGATGATTTGCGCTCTGTTTGCCTAGGTGAGGAC
GGTGCCTTTGCCGGAATGAAACCGGGTGCGATCTTTGTCGATCACACCACGGTATCAGCAGAGGTCACGGCAGAGCTCTA
CGCCCGCGCCAAAGACGCGCAGCTTGGGTTTGTCGACGCTCCGATCTCGGGCGGTCAGGCGGGGGCCGAGAATGGGGTTC
TTTCGGTGATGTGCGGCGGGGATGCTGCGGATTACGACAAAGCTGCGCCAGTGATGGAAGCCTATGCACGGATCTGCCGG
CGCATCGGTGAAAGTGGCGCAGGCCAGATGACCAAAATGTGCAACCAGATCGCCATTGCCGGCCTTGTGCAAGGTTTGAG
CGAAGCGCTTCATTTTGCGGAGAAAGCCGGGCTCGACGGGCGCGCGGTGGTCGAGGTGATCAGTCAGGGGGCAGCGGGCA
GCTGGCAGATGGCAAATCGCTATGAGACCATGCTCGACGATGAGTTCGAGCACGGGTTTGCCGTGGACTGGATGCGCAAG
GATCTCGGAATCTGCCTTGATACCGCCGACGACATTGGCGCAAGCCTGCCTGTCACGGCCTTGGTCGATCAGTTCTACAA
GGACGTTCAAAAACTGGGCGGCGGGCGTTGGGACACCTCATCCCTGATCAAACGGCTGCGGGCCTTGGGCTGA

Upstream 100 bases:

>100_bases
CTTGGTATTTCTGTGTTTGTTGCCAAGCCGCATTGCGCTTTGGGCGGGTGCTGCTAGGGTTGGCGCCAACATATGCGAAG
ACAGAACAGGGGGAAAGGAC

Downstream 100 bases:

>100_bases
AAAAAAGCCTCCCCTCGAATGAGGGGAGGTCTTCATCACCTTGATATAAAACAAAATCAGGGAATGATACGTGTGTATTT
GGAGCCCTCGATGGTGGCTC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MAKIAFLGLGVMGYPMAGHLQKAGHEVTVYNRTTAKAEGWAAATGGRSAPTPREAAQNADFVMACVGNDDDLRSVCLGED
GAFAGMKPGAIFVDHTTVSAEVTAELYARAKDAQLGFVDAPISGGQAGAENGVLSVMCGGDAADYDKAAPVMEAYARICR
RIGESGAGQMTKMCNQIAIAGLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMANRYETMLDDEFEHGFAVDWMRK
DLGICLDTADDIGASLPVTALVDQFYKDVQKLGGGRWDTSSLIKRLRALG

Sequences:

>Translated_290_residues
MAKIAFLGLGVMGYPMAGHLQKAGHEVTVYNRTTAKAEGWAAATGGRSAPTPREAAQNADFVMACVGNDDDLRSVCLGED
GAFAGMKPGAIFVDHTTVSAEVTAELYARAKDAQLGFVDAPISGGQAGAENGVLSVMCGGDAADYDKAAPVMEAYARICR
RIGESGAGQMTKMCNQIAIAGLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMANRYETMLDDEFEHGFAVDWMRK
DLGICLDTADDIGASLPVTALVDQFYKDVQKLGGGRWDTSSLIKRLRALG
>Mature_289_residues
AKIAFLGLGVMGYPMAGHLQKAGHEVTVYNRTTAKAEGWAAATGGRSAPTPREAAQNADFVMACVGNDDDLRSVCLGEDG
AFAGMKPGAIFVDHTTVSAEVTAELYARAKDAQLGFVDAPISGGQAGAENGVLSVMCGGDAADYDKAAPVMEAYARICRR
IGESGAGQMTKMCNQIAIAGLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMANRYETMLDDEFEHGFAVDWMRKD
LGICLDTADDIGASLPVTALVDQFYKDVQKLGGGRWDTSSLIKRLRALG

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=287, Percent_Identity=29.2682926829268, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=26.4604810996564, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=36.7857142857143, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=32.8719723183391, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1790315, Length=288, Percent_Identity=30.5555555555556, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1789092, Length=294, Percent_Identity=28.2312925170068, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=26.1818181818182, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=29.4520547945205, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=29.4520547945205, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28574115, Length=284, Percent_Identity=24.2957746478873, Blast_Score=107, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30462; Mature: 30331

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIAFLGLGVMGYPMAGHLQKAGHEVTVYNRTTAKAEGWAAATGGRSAPTPREAAQNAD
CCEEEEECCCHHCCCCHHHHHHCCCEEEEEECCCCCCCCCEECCCCCCCCCHHHHHCCCC
FVMACVGNDDDLRSVCLGEDGAFAGMKPGAIFVDHTTVSAEVTAELYARAKDAQLGFVDA
EEEEEECCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEC
PISGGQAGAENGVLSVMCGGDAADYDKAAPVMEAYARICRRIGESGAGQMTKMCNQIAIA
CCCCCCCCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMANRYETMLDDEFEHGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
DLGICLDTADDIGASLPVTALVDQFYKDVQKLGGGRWDTSSLIKRLRALG
HHCEEEECHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKIAFLGLGVMGYPMAGHLQKAGHEVTVYNRTTAKAEGWAAATGGRSAPTPREAAQNAD
CEEEEECCCHHCCCCHHHHHHCCCEEEEEECCCCCCCCCEECCCCCCCCCHHHHHCCCC
FVMACVGNDDDLRSVCLGEDGAFAGMKPGAIFVDHTTVSAEVTAELYARAKDAQLGFVDA
EEEEEECCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEC
PISGGQAGAENGVLSVMCGGDAADYDKAAPVMEAYARICRRIGESGAGQMTKMCNQIAIA
CCCCCCCCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMANRYETMLDDEFEHGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
DLGICLDTADDIGASLPVTALVDQFYKDVQKLGGGRWDTSSLIKRLRALG
HHCEEEECHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA