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The map label for this gene is trmJ [H]

Identifier: 99081177

GI number: 99081177

Start: 1424076

End: 1424825

Strand: Reverse

Name: trmJ [H]

Synonym: TM1040_1336

Alternate gene names: 99081177

Gene position: 1424825-1424076 (Counterclockwise)

Preceding gene: 99081182

Following gene: 99081176

Centisome position: 44.51

GC content: 60.8

Gene sequence:

>750_bases
ATGCCCTCAGACCGTCCTCAACCTGACTTTGTGCTCGTGCGCCCTCAGATGGGTGAAAACATCGGTGGGGCCGCGCGGGC
CATGTGGAACTTTGGCCTCGACCGCATGCGTATCGTTGCGCCGCGCGATGGCTGGCCAAATCCCAAGGCCGTGGCCATGG
CCTCCGGAGCGGGGCGCCTCCTCGACGAAGCGCAACTGGCACAGGACCTGCCCGAAGCGCTGGCGGATTGCACCTATGTC
TTTGCCACCACGGCGCGCCCGCGCGAGCTGACCAAACCGGTGTTCAGCCCCGAAGGGGCAATGAAGAAGGCCGCAGAACT
GATGGTGCAGGGCGAGAAGGTTGCCGTTCTCTTTGGTCCGGAACGCGCCGGATTGGAAAACGATGACATTGCCAAGGCAA
ACGCCATCATCACCGTGCCGGTGAACCCGGAGTTTGCCTCGCTCAACCTTGCGCAATGTGTCTTGCTGACCGCCTATGAA
TGGCGCCGCCAGCAAGAGCAGGTCGCGCATGAGACCTTTGAAATCGGGAAATCCGAATGGGCAACCAATCAAGAGGTGGA
AGCCCTTGTGAACCACTATGAGGAGCGCCTGACGGAAGCGGGCTATTTCTTCCCGCCCGAAAAGGCCCCCGGCATGAAGA
CAGTGTTCCGCAACCTCTGGAGCCGGATGCAACTGACGCGCGCTGATGTGCAAATGCTACATGGGGTGATGCGACAGATG
GTCCGTTGGAAGCAGCGCGAAGAGGACTAA

Upstream 100 bases:

>100_bases
CAAGGATTTGGGCACGGATCGCGTGGGTTTTCCCACCCCGAAGCCTTGCCCCAACCCGGCGCAGACAGTAAGGCAGAGCC
GATTTTTGCGGAGTTCCAAG

Downstream 100 bases:

>100_bases
CTCTCGCTGGACCTTTCCACCCTCAGACCCTAGCGTCCTGCAAGATTAAGATGTGAGGCCAAGATGAGCAATCGCACGAT
TTTTGAGGAAGTCTCCTCCG

Product: RNA methyltransferase TrmH, group 1

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPSDRPQPDFVLVRPQMGENIGGAARAMWNFGLDRMRIVAPRDGWPNPKAVAMASGAGRLLDEAQLAQDLPEALADCTYV
FATTARPRELTKPVFSPEGAMKKAAELMVQGEKVAVLFGPERAGLENDDIAKANAIITVPVNPEFASLNLAQCVLLTAYE
WRRQQEQVAHETFEIGKSEWATNQEVEALVNHYEERLTEAGYFFPPEKAPGMKTVFRNLWSRMQLTRADVQMLHGVMRQM
VRWKQREED

Sequences:

>Translated_249_residues
MPSDRPQPDFVLVRPQMGENIGGAARAMWNFGLDRMRIVAPRDGWPNPKAVAMASGAGRLLDEAQLAQDLPEALADCTYV
FATTARPRELTKPVFSPEGAMKKAAELMVQGEKVAVLFGPERAGLENDDIAKANAIITVPVNPEFASLNLAQCVLLTAYE
WRRQQEQVAHETFEIGKSEWATNQEVEALVNHYEERLTEAGYFFPPEKAPGMKTVFRNLWSRMQLTRADVQMLHGVMRQM
VRWKQREED
>Mature_248_residues
PSDRPQPDFVLVRPQMGENIGGAARAMWNFGLDRMRIVAPRDGWPNPKAVAMASGAGRLLDEAQLAQDLPEALADCTYVF
ATTARPRELTKPVFSPEGAMKKAAELMVQGEKVAVLFGPERAGLENDDIAKANAIITVPVNPEFASLNLAQCVLLTAYEW
RRQQEQVAHETFEIGKSEWATNQEVEALVNHYEERLTEAGYFFPPEKAPGMKTVFRNLWSRMQLTRADVQMLHGVMRQMV
RWKQREED

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=237, Percent_Identity=33.7552742616034, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790865, Length=151, Percent_Identity=33.7748344370861, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28042; Mature: 27911

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSDRPQPDFVLVRPQMGENIGGAARAMWNFGLDRMRIVAPRDGWPNPKAVAMASGAGRL
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCHHH
LDEAQLAQDLPEALADCTYVFATTARPRELTKPVFSPEGAMKKAAELMVQGEKVAVLFGP
HHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEECC
ERAGLENDDIAKANAIITVPVNPEFASLNLAQCVLLTAYEWRRQQEQVAHETFEIGKSEW
CCCCCCCCCCCCCCEEEEEECCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHC
ATNQEVEALVNHYEERLTEAGYFFPPEKAPGMKTVFRNLWSRMQLTRADVQMLHGVMRQM
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRWKQREED
HHHHHHCCC
>Mature Secondary Structure 
PSDRPQPDFVLVRPQMGENIGGAARAMWNFGLDRMRIVAPRDGWPNPKAVAMASGAGRL
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCHHH
LDEAQLAQDLPEALADCTYVFATTARPRELTKPVFSPEGAMKKAAELMVQGEKVAVLFGP
HHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEECC
ERAGLENDDIAKANAIITVPVNPEFASLNLAQCVLLTAYEWRRQQEQVAHETFEIGKSEW
CCCCCCCCCCCCCCEEEEEECCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHC
ATNQEVEALVNHYEERLTEAGYFFPPEKAPGMKTVFRNLWSRMQLTRADVQMLHGVMRQM
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRWKQREED
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]