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The map label for this gene is emrE [H]

Identifier: 99081077

GI number: 99081077

Start: 1332636

End: 1332965

Strand: Reverse

Name: emrE [H]

Synonym: TM1040_1236

Alternate gene names: 99081077

Gene position: 1332965-1332636 (Counterclockwise)

Preceding gene: 99081078

Following gene: 99081076

Centisome position: 41.64

GC content: 54.85

Gene sequence:

>330_bases
ATGCATTATCTTTACCTGATGGCCGCCGTTTTGGCCGAAACCATTGGGACAACCGCACTTCAGGCGAGCCAGCAATTCAC
CCGGCTGCTGCCGTCTGTGATTGTTGTCGTTGCTTACGGCCTGTCTTTCTTTCTGATGAGCCTCACGCTCAAGGTGATGC
CAGTGGGGATCGTGTATGCGATCTGGTCCGGGCTCGGGATTGTCCTGATCGCCACCATCGGCTTTCTTGTCTTTGGCCAA
CGCATTGATCTGCCTGCAATTGCGGGAATGGCGTTGATCATCACAGGCATTCTGGTGATCCACCTGTTCTCCAGCAGCTC
TGGCCACTGA

Upstream 100 bases:

>100_bases
GCCTGCATTTCCCCTCTTGCGTCGGCGCGCATGGCAGGATTTGAGGGGAGCATGCCCTGCCCGCTTGAGGTGGGACTCTC
AGGACCGATGAGGACATGCC

Downstream 100 bases:

>100_bases
TACCCGCCGCAAAGACCAGGATCACGCGGCAGTGCGGCGACAGTTGTGGCTTTGTCCCTCTTTTGCACTAGCCATGGGGG
CTTTATCGCGGTATGCGCCC

Product: small multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux-multidrug resistance protein emrE; Ethidium resistance protein; Methyl viologen resistance protein C [H]

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MHYLYLMAAVLAETIGTTALQASQQFTRLLPSVIVVVAYGLSFFLMSLTLKVMPVGIVYAIWSGLGIVLIATIGFLVFGQ
RIDLPAIAGMALIITGILVIHLFSSSSGH

Sequences:

>Translated_109_residues
MHYLYLMAAVLAETIGTTALQASQQFTRLLPSVIVVVAYGLSFFLMSLTLKVMPVGIVYAIWSGLGIVLIATIGFLVFGQ
RIDLPAIAGMALIITGILVIHLFSSSSGH
>Mature_109_residues
MHYLYLMAAVLAETIGTTALQASQQFTRLLPSVIVVVAYGLSFFLMSLTLKVMPVGIVYAIWSGLGIVLIATIGFLVFGQ
RIDLPAIAGMALIITGILVIHLFSSSSGH

Specific function: Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistanc

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=107, Percent_Identity=55.1401869158878, Blast_Score=118, Evalue=1e-28,
Organism=Escherichia coli, GI87082378, Length=102, Percent_Identity=36.2745098039216, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 11625; Mature: 11625

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYLYLMAAVLAETIGTTALQASQQFTRLLPSVIVVVAYGLSFFLMSLTLKVMPVGIVYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWSGLGIVLIATIGFLVFGQRIDLPAIAGMALIITGILVIHLFSSSSGH
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHYLYLMAAVLAETIGTTALQASQQFTRLLPSVIVVVAYGLSFFLMSLTLKVMPVGIVYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWSGLGIVLIATIGFLVFGQRIDLPAIAGMALIITGILVIHLFSSSSGH
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1936950; 1320256; 9278503; 7896833; 9050242; 9688273 [H]