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The map label for this gene is pleD [H]

Identifier: 99081010

GI number: 99081010

Start: 1257343

End: 1259133

Strand: Reverse

Name: pleD [H]

Synonym: TM1040_1169

Alternate gene names: 99081010

Gene position: 1259133-1257343 (Counterclockwise)

Preceding gene: 99081011

Following gene: 99081009

Centisome position: 39.34

GC content: 60.13

Gene sequence:

>1791_bases
ATGAAAATCAATACGATCACGAATTGGGCCTACGGATTGACTGTTCTTCTGACCGCGTTGTCGGGCGCCGCGTTCATCGC
GTCGATCAACAGCTCGGCCGAAGAACGCATGGCGGTTGAAACACATCTAATCCTGAACGAGTTGGGCGAAGAGCTTGCGA
TCGGAGCGGAGCTGCGCACCGACGAGGCACGGCTTTATGTGATGCGCGGCGACCCGGATCATCTTGAGGCTTTTGAGCGG
ACCGATCAGGCCGAAATGGCGCTGGAAGAGTCCGCCCGCGACGGCACGCGTCTTGGCACCACCCCGGAAGAGACCGCATT
TCTGAATCGGATCATCGCGGATATAGATGCGCTGCAAGAGATGGAGCGTGCGGCAATCAAAGCTTATCAAGCGGGCAACA
TTGACCGCGCGCGGGGGCTCTTGTTCGGGGGCGCGCATTACCAGGCCCACCTCAAACTCATTGACGATGTAGTCCAGTTC
CGCCGGATGGTGCAAAGCCGGACAGACCGTGAGCTCGACACCGCACATCAACGCAGCGACTGGTTCGAATTCGTTGCGCA
GATCTCCCTCGCGCTCACCGCCACCGTTTTCCTCGGCGTACTCTATTTTGTCCTGCGTCGCCGGGTGGCGGTGCCCCTGG
CACGCATGGCCGGCATCGTAAAGCGCCTCGCCAGCCAGGACTACGAGGTCGAGGTGCCATTGGATCGCAGACGGGACGAG
ATTGGTGAGCTCAATCGCGCCGTGCATGTGTTCCGCGAAAACGGGCTCGAGCGGGAACGTCTCGACGCCGAGCGCCGCCG
TGACATCAAGATCAAGGATCTGATCCTGAAGCTCATGCACCGGGTTCAGGCCTGCCAGTCTCTCGACGAACTGGGGGATG
TCCTTGCGCGGTATGCGCCGCAGATCTTTCCCGACCTCTCCGGCACGCTCTACCTGCGCGCTGACGGTCACGAGACCTTG
AACTGTGTGTCCCGATGGCAGACGTCATTCGACGACCCGCTCGAGATCATCTGCGAGGCCTGCTGGGCCCTGCGCCGCGG
ACGCGCTCATTATAGCGCCTTGGAATCAAAAGAAGATGTGATTTGCAACCATCTCGCTGACCCGAACGTTTCGACCCTGT
GCATTCCGCTCGCTGCTCAGGGCGAGACCATCGGCCTTCTGAGTTTCTCTGGCGCCGAGACCTCGACAGAGGTTGCGCGC
GAAGATCGGGTCTATCTGGAGCTTATTGCTGAAAACGTCGCGCTTGCGGCCGTGAACCTCAATCTGCGCACCCGGCTCTC
GCAGCTGGTCGAACATGATCCTCTGACCGGGCTTTTGAACCGGCGCTCGCTGGATGTGGCCTTTGGCGATTTTGTGCAAA
ACCCGCCAACCCTTCCGACCGCCTGCCTGATGATCGACATTGATCACTTCAAGCGGATCAATGATGAGTTTGGCCATGAG
GCCGGGGATACGGTGATGCAAAGCTTTGCCGGGTTACTCCGCGAGGTCGTGGGCGACAGCGGCTCCTGTTACCGCTTTGG
CGGCGAGGAATTTGTGGTGATCCTGCCGGATCACGACGTCGATGCCGCGCATGAGATCGCCGAAGGCATTCGCACCCGCA
CCGCAAAGATGGCCATTGCCCATCGCGGGCAATCTCTTGGGAAGGTCACGATTTCCATCGGTCTTGCGGTTGCAGCATCC
GCAGGCACCCTCGACACCTTGTTGAGCCGCGCGGATTCAGCGCTGCTGCAGGCCAAGGTGACGGGTCGCAACATCACGTT
GCACGAGACACTTGAGCTCGCCCCTTCCTGA

Upstream 100 bases:

>100_bases
GGCGAATTTCCTTTCGCGGGACTTCTTCAAAAACTCTTATATTTAAATTATCCGTAAATTCGATTCCCTGCCGGGGTCTT
GTCAATTTTGGAACTTTCGA

Downstream 100 bases:

>100_bases
GGAACAGCGACGACCCGAGACGCCCTCGGATGTCTCTACAGACCAGGACACCCCCCAGGAACTGAAAAACCGCCGATCTT
ACGACCGGCGGTTTTCTCAT

Product: diguanylate cyclase with GAF sensor

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKINTITNWAYGLTVLLTALSGAAFIASINSSAEERMAVETHLILNELGEELAIGAELRTDEARLYVMRGDPDHLEAFER
TDQAEMALEESARDGTRLGTTPEETAFLNRIIADIDALQEMERAAIKAYQAGNIDRARGLLFGGAHYQAHLKLIDDVVQF
RRMVQSRTDRELDTAHQRSDWFEFVAQISLALTATVFLGVLYFVLRRRVAVPLARMAGIVKRLASQDYEVEVPLDRRRDE
IGELNRAVHVFRENGLERERLDAERRRDIKIKDLILKLMHRVQACQSLDELGDVLARYAPQIFPDLSGTLYLRADGHETL
NCVSRWQTSFDDPLEIICEACWALRRGRAHYSALESKEDVICNHLADPNVSTLCIPLAAQGETIGLLSFSGAETSTEVAR
EDRVYLELIAENVALAAVNLNLRTRLSQLVEHDPLTGLLNRRSLDVAFGDFVQNPPTLPTACLMIDIDHFKRINDEFGHE
AGDTVMQSFAGLLREVVGDSGSCYRFGGEEFVVILPDHDVDAAHEIAEGIRTRTAKMAIAHRGQSLGKVTISIGLAVAAS
AGTLDTLLSRADSALLQAKVTGRNITLHETLELAPS

Sequences:

>Translated_596_residues
MKINTITNWAYGLTVLLTALSGAAFIASINSSAEERMAVETHLILNELGEELAIGAELRTDEARLYVMRGDPDHLEAFER
TDQAEMALEESARDGTRLGTTPEETAFLNRIIADIDALQEMERAAIKAYQAGNIDRARGLLFGGAHYQAHLKLIDDVVQF
RRMVQSRTDRELDTAHQRSDWFEFVAQISLALTATVFLGVLYFVLRRRVAVPLARMAGIVKRLASQDYEVEVPLDRRRDE
IGELNRAVHVFRENGLERERLDAERRRDIKIKDLILKLMHRVQACQSLDELGDVLARYAPQIFPDLSGTLYLRADGHETL
NCVSRWQTSFDDPLEIICEACWALRRGRAHYSALESKEDVICNHLADPNVSTLCIPLAAQGETIGLLSFSGAETSTEVAR
EDRVYLELIAENVALAAVNLNLRTRLSQLVEHDPLTGLLNRRSLDVAFGDFVQNPPTLPTACLMIDIDHFKRINDEFGHE
AGDTVMQSFAGLLREVVGDSGSCYRFGGEEFVVILPDHDVDAAHEIAEGIRTRTAKMAIAHRGQSLGKVTISIGLAVAAS
AGTLDTLLSRADSALLQAKVTGRNITLHETLELAPS
>Mature_596_residues
MKINTITNWAYGLTVLLTALSGAAFIASINSSAEERMAVETHLILNELGEELAIGAELRTDEARLYVMRGDPDHLEAFER
TDQAEMALEESARDGTRLGTTPEETAFLNRIIADIDALQEMERAAIKAYQAGNIDRARGLLFGGAHYQAHLKLIDDVVQF
RRMVQSRTDRELDTAHQRSDWFEFVAQISLALTATVFLGVLYFVLRRRVAVPLARMAGIVKRLASQDYEVEVPLDRRRDE
IGELNRAVHVFRENGLERERLDAERRRDIKIKDLILKLMHRVQACQSLDELGDVLARYAPQIFPDLSGTLYLRADGHETL
NCVSRWQTSFDDPLEIICEACWALRRGRAHYSALESKEDVICNHLADPNVSTLCIPLAAQGETIGLLSFSGAETSTEVAR
EDRVYLELIAENVALAAVNLNLRTRLSQLVEHDPLTGLLNRRSLDVAFGDFVQNPPTLPTACLMIDIDHFKRINDEFGHE
AGDTVMQSFAGLLREVVGDSGSCYRFGGEEFVVILPDHDVDAAHEIAEGIRTRTAKMAIAHRGQSLGKVTISIGLAVAAS
AGTLDTLLSRADSALLQAKVTGRNITLHETLELAPS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2770

COG function: function code T; FOG: HAMP domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=38.0952380952381, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787816, Length=200, Percent_Identity=32.5, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI145693134, Length=155, Percent_Identity=35.4838709677419, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787262, Length=216, Percent_Identity=33.7962962962963, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=35.2601156069364, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787802, Length=186, Percent_Identity=33.8709677419355, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=34.1317365269461, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=31.9526627218935, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788085, Length=161, Percent_Identity=29.8136645962733, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=31.1111111111111, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787056, Length=152, Percent_Identity=32.8947368421053, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081921, Length=208, Percent_Identity=27.8846153846154, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 66133; Mature: 66133

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINTITNWAYGLTVLLTALSGAAFIASINSSAEERMAVETHLILNELGEELAIGAELRT
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
DEARLYVMRGDPDHLEAFERTDQAEMALEESARDGTRLGTTPEETAFLNRIIADIDALQE
CCEEEEEEECCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MERAAIKAYQAGNIDRARGLLFGGAHYQAHLKLIDDVVQFRRMVQSRTDRELDTAHQRSD
HHHHHHHHHHCCCCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WFEFVAQISLALTATVFLGVLYFVLRRRVAVPLARMAGIVKRLASQDYEVEVPLDRRRDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHH
IGELNRAVHVFRENGLERERLDAERRRDIKIKDLILKLMHRVQACQSLDELGDVLARYAP
HHHHHHHHHHHHHCCCCHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
QIFPDLSGTLYLRADGHETLNCVSRWQTSFDDPLEIICEACWALRRGRAHYSALESKEDV
HHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHH
ICNHLADPNVSTLCIPLAAQGETIGLLSFSGAETSTEVAREDRVYLELIAENVALAAVNL
HHHHCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEC
NLRTRLSQLVEHDPLTGLLNRRSLDVAFGDFVQNPPTLPTACLMIDIDHFKRINDEFGHE
HHHHHHHHHHHCCCCHHHHCCCCCCEEHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHCCC
AGDTVMQSFAGLLREVVGDSGSCYRFGGEEFVVILPDHDVDAAHEIAEGIRTRTAKMAIA
HHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
HRGQSLGKVTISIGLAVAASAGTLDTLLSRADSALLQAKVTGRNITLHETLELAPS
HCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHEEECCCEEEEEHHHCCCCC
>Mature Secondary Structure
MKINTITNWAYGLTVLLTALSGAAFIASINSSAEERMAVETHLILNELGEELAIGAELRT
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
DEARLYVMRGDPDHLEAFERTDQAEMALEESARDGTRLGTTPEETAFLNRIIADIDALQE
CCEEEEEEECCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MERAAIKAYQAGNIDRARGLLFGGAHYQAHLKLIDDVVQFRRMVQSRTDRELDTAHQRSD
HHHHHHHHHHCCCCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WFEFVAQISLALTATVFLGVLYFVLRRRVAVPLARMAGIVKRLASQDYEVEVPLDRRRDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHH
IGELNRAVHVFRENGLERERLDAERRRDIKIKDLILKLMHRVQACQSLDELGDVLARYAP
HHHHHHHHHHHHHCCCCHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
QIFPDLSGTLYLRADGHETLNCVSRWQTSFDDPLEIICEACWALRRGRAHYSALESKEDV
HHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHH
ICNHLADPNVSTLCIPLAAQGETIGLLSFSGAETSTEVAREDRVYLELIAENVALAAVNL
HHHHCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEC
NLRTRLSQLVEHDPLTGLLNRRSLDVAFGDFVQNPPTLPTACLMIDIDHFKRINDEFGHE
HHHHHHHHHHHCCCCHHHHCCCCCCEEHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHCCC
AGDTVMQSFAGLLREVVGDSGSCYRFGGEEFVVILPDHDVDAAHEIAEGIRTRTAKMAIA
HHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
HRGQSLGKVTISIGLAVAASAGTLDTLLSRADSALLQAKVTGRNITLHETLELAPS
HCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHEEECCCEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA