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The map label for this gene is rluE [H]

Identifier: 99081002

GI number: 99081002

Start: 1250925

End: 1251590

Strand: Reverse

Name: rluE [H]

Synonym: TM1040_1161

Alternate gene names: 99081002

Gene position: 1251590-1250925 (Counterclockwise)

Preceding gene: 99081004

Following gene: 99081000

Centisome position: 39.1

GC content: 63.51

Gene sequence:

>666_bases
ATGAGCCGCTTGATCCGTTTCAACAAACCCTTTGATGTGCTGCCGCAGTTCACCGATCGTGGCAGCGAGGGCAGCCCGCG
CGCAACGCTTAGCGATTATGTTGACTGTCCCGGCGTCTATCCCGCAGGGCGTCTGGACCGCGACAGCGAAGGGCTGATGC
TGCTCACAGACAATGGCCGCTTGCAGGCCTGGATCAGCGACCCAAAGCATAAGCTCGAGAAAACCTATTGGGTTCAGGTG
GAAGGCATCCCCACCGAGGACGCTTTGAAGGCCCTGCGCCAAGGCGTTGATTTGAAAGACGGAATGACACGCCCCGCAAA
GGCGGAACTGATCTCGGAACCCGCACAGCTCTGGGCGCGCACGCCGCCGATCCGAGAGCGCAAATCCGTGCCGACCGCTT
GGCTCGAACTGAAGATATCCGAAGGCAAAAACCGGCAGGTGCGGCGGATGACGGCCGCTATGGGCTATCCGACCCTGCGT
CTCATCCGCTATGCCATTGGCGCCTGGACGCTCGATGGTCTGGCGCAGGGCAAATGGGACGATCTTGACCCGCCCCGCGT
GCCCGGCCCGCCAAAGCCCGCGGCCAAACCGCATCGCAAGCATGCCGCAAAACGGCGCGCCACGGCGACACCTGCCCGCA
AGCCCCGGCAGCGCCGCGACGGCTAA

Upstream 100 bases:

>100_bases
GGGCGTGACAGTTATCCACAGCCCAAAAAACAGGCGTTCTGCGCTGCGGCACCGATGCCCGGGCTTGCACCGCGCCCCGC
CTGCCCGTAGAGCGATCCTC

Downstream 100 bases:

>100_bases
TCAGATCAGATGCTGCCCTCAGCTTCGATCACGAGGACACGCGCCGCACCCCGCGGATGCGCCACATGCTCGTCCCCGGC
CTCGGCGTGAAAGATATCCC

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSRLIRFNKPFDVLPQFTDRGSEGSPRATLSDYVDCPGVYPAGRLDRDSEGLMLLTDNGRLQAWISDPKHKLEKTYWVQV
EGIPTEDALKALRQGVDLKDGMTRPAKAELISEPAQLWARTPPIRERKSVPTAWLELKISEGKNRQVRRMTAAMGYPTLR
LIRYAIGAWTLDGLAQGKWDDLDPPRVPGPPKPAAKPHRKHAAKRRATATPARKPRQRRDG

Sequences:

>Translated_221_residues
MSRLIRFNKPFDVLPQFTDRGSEGSPRATLSDYVDCPGVYPAGRLDRDSEGLMLLTDNGRLQAWISDPKHKLEKTYWVQV
EGIPTEDALKALRQGVDLKDGMTRPAKAELISEPAQLWARTPPIRERKSVPTAWLELKISEGKNRQVRRMTAAMGYPTLR
LIRYAIGAWTLDGLAQGKWDDLDPPRVPGPPKPAAKPHRKHAAKRRATATPARKPRQRRDG
>Mature_220_residues
SRLIRFNKPFDVLPQFTDRGSEGSPRATLSDYVDCPGVYPAGRLDRDSEGLMLLTDNGRLQAWISDPKHKLEKTYWVQVE
GIPTEDALKALRQGVDLKDGMTRPAKAELISEPAQLWARTPPIRERKSVPTAWLELKISEGKNRQVRRMTAAMGYPTLRL
IRYAIGAWTLDGLAQGKWDDLDPPRVPGPPKPAAKPHRKHAAKRRATATPARKPRQRRDG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=61.878453038674, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1788510, Length=144, Percent_Identity=37.5, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790453, Length=180, Percent_Identity=30.5555555555556, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787524, Length=185, Percent_Identity=31.3513513513514, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 24904; Mature: 24773

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIRFNKPFDVLPQFTDRGSEGSPRATLSDYVDCPGVYPAGRLDRDSEGLMLLTDNGR
CCCCCCCCCCHHHCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCE
LQAWISDPKHKLEKTYWVQVEGIPTEDALKALRQGVDLKDGMTRPAKAELISEPAQLWAR
EEEEECCHHHHHCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
TPPIRERKSVPTAWLELKISEGKNRQVRRMTAAMGYPTLRLIRYAIGAWTLDGLAQGKWD
CCCCHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
DLDPPRVPGPPKPAAKPHRKHAAKRRATATPARKPRQRRDG
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
SRLIRFNKPFDVLPQFTDRGSEGSPRATLSDYVDCPGVYPAGRLDRDSEGLMLLTDNGR
CCCCCCCCCHHHCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCE
LQAWISDPKHKLEKTYWVQVEGIPTEDALKALRQGVDLKDGMTRPAKAELISEPAQLWAR
EEEEECCHHHHHCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
TPPIRERKSVPTAWLELKISEGKNRQVRRMTAAMGYPTLRLIRYAIGAWTLDGLAQGKWD
CCCCHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
DLDPPRVPGPPKPAAKPHRKHAAKRRATATPARKPRQRRDG
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]