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The map label for this gene is lexA

Identifier: 99080976

GI number: 99080976

Start: 1221954

End: 1222679

Strand: Reverse

Name: lexA

Synonym: TM1040_1135

Alternate gene names: 99080976

Gene position: 1222679-1221954 (Counterclockwise)

Preceding gene: 99080977

Following gene: 99080974

Centisome position: 38.2

GC content: 63.36

Gene sequence:

>726_bases
ATGCTGACGAAAAAACAGCTTCAGCTACTGGAGTTTATTCACAAGCGTCTGCAGAAGGATGGCGTCCCTCCCAGTTTTGA
TGAGATGAAAACCGCGTTGGATCTGCGGTCCAAGTCCGGCATTCACCGTCTGATTACGGCGCTGGAAGAGCGCGGCTTTA
TCCGCCGCCTTGCCCATCGCGCGCGCGCCATCGAGGTGATCCGCCTGCCCGACAGTCTGGGCGGTGGCGGCGCGCTTGGT
GCTGCGAGCGATGGTTTCCAGCCCAAAGTGATCGCCGGCGGCCGGGGTGACAGTGCCGACGGCGGCGCTGCGGCAGAATT
GAAACCCGTGGCAGATACCGGGGCGACGGAACTCACGATCATGGGCCGGATCGCAGCCGGTGTCCCGATCGAGGCGATCA
ACCAGGCCGCCGCCCATGTGGCGGTGCCCAATGCCATGCTCTCCAGCTCGGGTCAGCATTACGCCCTCGAGGTGCGCGGT
GACTCGATGATCGACGCCGGGATAAACGACGGCGATGTGGTGGTGATCCGCGAGACCGACGCGGCCGACAATGGCGACAT
TGTGGTGGCCCTCGTGGAGGGGCATGAGGCCACGCTCAAACGGTTTGAACGCAAGGGCCGCATGATCGAGCTTCACGCCG
CCAATCCGGCCTACCCCACCCGCAGCTATACCGAGGATCAAGTGAAGGTGCAGGGGCGTCTGGTGGGGCTGATCCGCACC
TACTGA

Upstream 100 bases:

>100_bases
TTAGCTGGGCTTTTTATGTTCCCCTTATGACCCGACCCGTTGACACAAAACGGGAACATGAATAGAACATAAACAACGAT
ATTGTGCAGAGGGGGCAGCC

Downstream 100 bases:

>100_bases
GCAAACACCTGCGTGCACCATTTCTCGCTCGGCCGCATATTCTGTGGCCGGGCGTTTTTGTCAGGGCGTTTATTCAGGGC
GCCGACCACAAGCGGCGCGC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MLTKKQLQLLEFIHKRLQKDGVPPSFDEMKTALDLRSKSGIHRLITALEERGFIRRLAHRARAIEVIRLPDSLGGGGALG
AASDGFQPKVIAGGRGDSADGGAAAELKPVADTGATELTIMGRIAAGVPIEAINQAAAHVAVPNAMLSSSGQHYALEVRG
DSMIDAGINDGDVVVIRETDAADNGDIVVALVEGHEATLKRFERKGRMIELHAANPAYPTRSYTEDQVKVQGRLVGLIRT
Y

Sequences:

>Translated_241_residues
MLTKKQLQLLEFIHKRLQKDGVPPSFDEMKTALDLRSKSGIHRLITALEERGFIRRLAHRARAIEVIRLPDSLGGGGALG
AASDGFQPKVIAGGRGDSADGGAAAELKPVADTGATELTIMGRIAAGVPIEAINQAAAHVAVPNAMLSSSGQHYALEVRG
DSMIDAGINDGDVVVIRETDAADNGDIVVALVEGHEATLKRFERKGRMIELHAANPAYPTRSYTEDQVKVQGRLVGLIRT
Y
>Mature_241_residues
MLTKKQLQLLEFIHKRLQKDGVPPSFDEMKTALDLRSKSGIHRLITALEERGFIRRLAHRARAIEVIRLPDSLGGGGALG
AASDGFQPKVIAGGRGDSADGGAAAELKPVADTGATELTIMGRIAAGVPIEAINQAAAHVAVPNAMLSSSGQHYALEVRG
DSMIDAGINDGDVVVIRETDAADNGDIVVALVEGHEATLKRFERKGRMIELHAANPAYPTRSYTEDQVKVQGRLVGLIRT
Y

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=241, Percent_Identity=24.0663900414938, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_SILST (Q1GHJ8)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613130.1
- ProteinModelPortal:   Q1GHJ8
- SMR:   Q1GHJ8
- STRING:   Q1GHJ8
- MEROPS:   S24.001
- GeneID:   4078431
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1135
- NMPDR:   fig|292414.1.peg.3730
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- BioCyc:   SSP292414:TM1040_1135-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25706; Mature: 25706

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: ACT_SITE 162-162 ACT_SITE 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKKQLQLLEFIHKRLQKDGVPPSFDEMKTALDLRSKSGIHRLITALEERGFIRRLAHR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHH
ARAIEVIRLPDSLGGGGALGAASDGFQPKVIAGGRGDSADGGAAAELKPVADTGATELTI
HHEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCEEEE
MGRIAAGVPIEAINQAAAHVAVPNAMLSSSGQHYALEVRGDSMIDAGINDGDVVVIRETD
EEHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCEEEEEECCCCEEECCCCCCCEEEEEECC
AADNGDIVVALVEGHEATLKRFERKGRMIELHAANPAYPTRSYTEDQVKVQGRLVGLIRT
CCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEHHEEEEEEEC
Y
C
>Mature Secondary Structure
MLTKKQLQLLEFIHKRLQKDGVPPSFDEMKTALDLRSKSGIHRLITALEERGFIRRLAHR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHH
ARAIEVIRLPDSLGGGGALGAASDGFQPKVIAGGRGDSADGGAAAELKPVADTGATELTI
HHEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCEEEE
MGRIAAGVPIEAINQAAAHVAVPNAMLSSSGQHYALEVRGDSMIDAGINDGDVVVIRETD
EEHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCEEEEEECCCCEEECCCCCCCEEEEEECC
AADNGDIVVALVEGHEATLKRFERKGRMIELHAANPAYPTRSYTEDQVKVQGRLVGLIRT
CCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEHHEEEEEEEC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA