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The map label for this gene is hipO [H]

Identifier: 99080560

GI number: 99080560

Start: 776435

End: 777598

Strand: Direct

Name: hipO [H]

Synonym: TM1040_0719

Alternate gene names: 99080560

Gene position: 776435-777598 (Clockwise)

Preceding gene: 99080559

Following gene: 99080566

Centisome position: 24.26

GC content: 59.97

Gene sequence:

>1164_bases
ATGCCCGTCAAGAACCGCTTTGCTGAGTTGCTGCCCGAAATTACTGCGTGGCGACGTGACCTGCACGAAAACCCCGAAAT
CCTGTTCGAAACCCATCGCACCAGCGCGCTGGTGGAGGAGAAACTCAAGGCGTTCGGCTGCGACGAAGTGGTCACGGGCA
TTGGCCGCACCGGCGTTGTGGGCGTCATCAAGGGTAAATCCTCCGCATCAGGCAAGGTCATCGGTCTGCGGGCCGATATG
GACGCGCTGCCGATCCATGAAGAAACCGGGCTGGAGTATGCCTCCAAGACCGCAAACGCCATGCACGCCTGTGGTCATGA
CGGTCATACCGCCATGCTTTTGGGCGCGGCGAAATATCTCTCCGAGACGCGGAACTTCGACGGCACCGTTGTGGTGATCT
TTCAGCCTGCCGAAGAAGGCGGCGGCGGCGGCAAGGAAATGTGCGATGATGGCATGATGGAGCGCTGGGGCATCCAGGAA
GTCTATGGCATGCACAATTGGCCGGGTCGCCCGGTTGGAAGCTTTGCAATCCGTTCGGGTGCCTTCTTTGCGGCGACCGA
TCAGTTCGACATCACCTTTACCGGCAAAGGCGGCCATGCCGCTAAGCCGCAGGAAACCATCGATTCGACCGTGATGGCAT
CGCAGGCGGTGCTTGCCCTGCAAACCATCGCTGCCCGCAACGCCGATCCCGTGCATCAGATCGTGGTCTCTGTGACCTCT
TTTGAGACCTCCTCCAAGGCGTTCAACGTGATTCCTGAGCGCGTTCAGATCAAAGGCACCGTGCGCACCATGGCGCCCGA
GATGCGGGATCTTGCTGAAAAACGTATCAAGGAAATCTGCGCGGGCATCGCAGCGACCTTTGGCGGTGAAGCCGATGTGA
CTTACCACCGTGGCTATCCGGTGATGGTGAACCATGACGAGCAGACCGAGTTTGCCGCCAAAGTGGCGCGTGACATTTCC
GGGCAGTGCGATGAGGCGCCGCTGGTGATGGGGGGCGAAGACTTTGCCTTCATGCTCGAAGAGCGTCCCGGTGCCTATAT
TCTCGTCGGCAATGGGGACACCGCCGCCGTGCATCACCCCAAGTATAACTTCACCGATGATGCGATTCCCGCAGGCTGCA
GCTGGTGGGCGGAGATCGTCGAGCAGCGCATGCCCGCAGCCTGA

Upstream 100 bases:

>100_bases
TGCCCGCCAGCTCATCATAAGGGCGCTGCGTTGCAGCCCCTGTGCACCCGGCCGCTCCCCGTGCCGGGTGTGACCATCCG
AAAGACAAGGAGAAAGACCT

Downstream 100 bases:

>100_bases
TCCGTATCATCAAAGTGAAGAAGGGGGCCACTTCGGCCCCCTTTTTTGTGCAACCGCTTGGGCTTTTGTCTCACCTTCCC
AGCTGCACTCTCGTAAGGAT

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPVKNRFAELLPEITAWRRDLHENPEILFETHRTSALVEEKLKAFGCDEVVTGIGRTGVVGVIKGKSSASGKVIGLRADM
DALPIHEETGLEYASKTANAMHACGHDGHTAMLLGAAKYLSETRNFDGTVVVIFQPAEEGGGGGKEMCDDGMMERWGIQE
VYGMHNWPGRPVGSFAIRSGAFFAATDQFDITFTGKGGHAAKPQETIDSTVMASQAVLALQTIAARNADPVHQIVVSVTS
FETSSKAFNVIPERVQIKGTVRTMAPEMRDLAEKRIKEICAGIAATFGGEADVTYHRGYPVMVNHDEQTEFAAKVARDIS
GQCDEAPLVMGGEDFAFMLEERPGAYILVGNGDTAAVHHPKYNFTDDAIPAGCSWWAEIVEQRMPAA

Sequences:

>Translated_387_residues
MPVKNRFAELLPEITAWRRDLHENPEILFETHRTSALVEEKLKAFGCDEVVTGIGRTGVVGVIKGKSSASGKVIGLRADM
DALPIHEETGLEYASKTANAMHACGHDGHTAMLLGAAKYLSETRNFDGTVVVIFQPAEEGGGGGKEMCDDGMMERWGIQE
VYGMHNWPGRPVGSFAIRSGAFFAATDQFDITFTGKGGHAAKPQETIDSTVMASQAVLALQTIAARNADPVHQIVVSVTS
FETSSKAFNVIPERVQIKGTVRTMAPEMRDLAEKRIKEICAGIAATFGGEADVTYHRGYPVMVNHDEQTEFAAKVARDIS
GQCDEAPLVMGGEDFAFMLEERPGAYILVGNGDTAAVHHPKYNFTDDAIPAGCSWWAEIVEQRMPAA
>Mature_386_residues
PVKNRFAELLPEITAWRRDLHENPEILFETHRTSALVEEKLKAFGCDEVVTGIGRTGVVGVIKGKSSASGKVIGLRADMD
ALPIHEETGLEYASKTANAMHACGHDGHTAMLLGAAKYLSETRNFDGTVVVIFQPAEEGGGGGKEMCDDGMMERWGIQEV
YGMHNWPGRPVGSFAIRSGAFFAATDQFDITFTGKGGHAAKPQETIDSTVMASQAVLALQTIAARNADPVHQIVVSVTSF
ETSSKAFNVIPERVQIKGTVRTMAPEMRDLAEKRIKEICAGIAATFGGEADVTYHRGYPVMVNHDEQTEFAAKVARDISG
QCDEAPLVMGGEDFAFMLEERPGAYILVGNGDTAAVHHPKYNFTDDAIPAGCSWWAEIVEQRMPAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=425, Percent_Identity=27.5294117647059, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41847; Mature: 41716

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKNRFAELLPEITAWRRDLHENPEILFETHRTSALVEEKLKAFGCDEVVTGIGRTGVV
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GVIKGKSSASGKVIGLRADMDALPIHEETGLEYASKTANAMHACGHDGHTAMLLGAAKYL
EEEECCCCCCCCEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SETRNFDGTVVVIFQPAEEGGGGGKEMCDDGMMERWGIQEVYGMHNWPGRPVGSFAIRSG
HHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEECC
AFFAATDQFDITFTGKGGHAAKPQETIDSTVMASQAVLALQTIAARNADPVHQIVVSVTS
EEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FETSSKAFNVIPERVQIKGTVRTMAPEMRDLAEKRIKEICAGIAATFGGEADVTYHRGYP
CCCCCHHHHCCCCCEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VMVNHDEQTEFAAKVARDISGQCDEAPLVMGGEDFAFMLEERPGAYILVGNGDTAAVHHP
EEECCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEECCCCEEEEEECCCEEEEECC
KYNFTDDAIPAGCSWWAEIVEQRMPAA
CCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVKNRFAELLPEITAWRRDLHENPEILFETHRTSALVEEKLKAFGCDEVVTGIGRTGVV
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GVIKGKSSASGKVIGLRADMDALPIHEETGLEYASKTANAMHACGHDGHTAMLLGAAKYL
EEEECCCCCCCCEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SETRNFDGTVVVIFQPAEEGGGGGKEMCDDGMMERWGIQEVYGMHNWPGRPVGSFAIRSG
HHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEECC
AFFAATDQFDITFTGKGGHAAKPQETIDSTVMASQAVLALQTIAARNADPVHQIVVSVTS
EEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FETSSKAFNVIPERVQIKGTVRTMAPEMRDLAEKRIKEICAGIAATFGGEADVTYHRGYP
CCCCCHHHHCCCCCEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VMVNHDEQTEFAAKVARDISGQCDEAPLVMGGEDFAFMLEERPGAYILVGNGDTAAVHHP
EEECCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEECCCCEEEEEECCCEEEEECC
KYNFTDDAIPAGCSWWAEIVEQRMPAA
CCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]